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3. Proteins that control the geometry of microtubules at the ends of cilia

Author

Louka, Panagiota, Vasudevan, Krishna Kumar, Guha, Mayukh, Joachimiak, Ewa, Wloga, Dorota, Tomasi, Raphaël F-X, Baroud, Charles N, Dupuis-Williams, Pascale, Galati, Domenico F, Pearson, Chad G, Rice, Luke M, Moresco, James J, Yates, John R, Jiang, Yu-Yang, Lechtreck, Karl, Dentler, William, and Gaertig, Jacek

Subjects
Biochemistry and Cell Biology, Biological Sciences, Pediatric, Genetics, Abnormalities, Multiple, Armadillo Domain Proteins, Cell Cycle Proteins, Cerebellum, Cilia, Eye Abnormalities, Humans, Kidney Diseases, Cystic, Microtubules, Protozoan Proteins, Retina, Tetrahymena thermophila, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Cilia, essential motile and sensory organelles, have several compartments: the basal body, transition zone, and the middle and distal axoneme segments. The distal segment accommodates key functions, including cilium assembly and sensory activities. While the middle segment contains doublet microtubules (incomplete B-tubules fused to complete A-tubules), the distal segment contains only A-tubule extensions, and its existence requires coordination of microtubule length at the nanometer scale. We show that three conserved proteins, two of which are mutated in the ciliopathy Joubert syndrome, determine the geometry of the distal segment, by controlling the positions of specific microtubule ends. FAP256/CEP104 promotes A-tubule elongation. CHE-12/Crescerin and ARMC9 act as positive and negative regulators of B-tubule length, respectively. We show that defects in the distal segment dimensions are associated with motile and sensory deficiencies of cilia. Our observations suggest that abnormalities in distal segment organization cause a subset of Joubert syndrome cases.

Published
2018

4. Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules

Author

Stoddard, Daniel, Zhao, Ying, Bayless, Brian A, Gui, Long, Louka, Panagiota, Dave, Drashti, Suryawanshi, Swati, Tomasi, Raphaël F-X, Dupuis-Williams, Pascale, Baroud, Charles N, Gaertig, Jacek, Winey, Mark, and Nicastro, Daniela

Subjects
Biochemistry and Cell Biology, Biological Sciences, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Axoneme, Cilia, Fluorescence, Gene Knockout Techniques, Green Fluorescent Proteins, Microtubule Proteins, Microtubules, Models, Biological, Mutation, Phagocytosis, Protein Subunits, Protozoan Proteins, Tetrahymena, Video Recording, Medical and Health Sciences, Developmental Biology, Biochemistry and cell biology
Abstract

Doublet and triplet microtubules are essential and highly stable core structures of centrioles, basal bodies, cilia, and flagella. In contrast to dynamic cytoplasmic micro-tubules, their luminal surface is coated with regularly arranged microtubule inner proteins (MIPs). However, the protein composition and biological function(s) of MIPs remain poorly understood. Using genetic, biochemical, and imaging techniques, we identified Tetrahymena RIB72A and RIB72B proteins as ciliary MIPs. Fluorescence imaging of tagged RIB72A and RIB72B showed that both proteins colocalize to Tetrahymena cilia and basal bodies but assemble independently. Cryoelectron tomography of RIB72A and/or RIB72B knockout strains revealed major structural defects in the ciliary A-tubule involving MIP1, MIP4, and MIP6 structures. The defects of individual mutants were complementary in the double mutant. All mutants had reduced swimming speed and ciliary beat frequencies, and high-speed video imaging revealed abnormal highly curved cilia during power stroke. Our results show that RIB72A and RIB72B are crucial for the structural assembly of ciliary A-tubule MIPs and are important for proper ciliary motility.

Published
2018

30. Impact of Botrytis cinerea contamination on the characteristics and foamability of yeast macromolecules released during the alcoholic fermentation of a model grape juice

Author

Marchal, Richard, Salmon, Thomas, Gonzalez, Ramon, Kemp, Belinda, Vrigneau, Céline, Williams, Pascale, Doco, Thierry, Kemp, Belinda, Laboratoire d'Oenologie, Université de Reims Champagne-Ardenne (URCA), Laboratoire Vigne Biotechnologie et Environnement (LVBE), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Instituto de Ciencias de la Vid y el Vino (ICVV), Universidad de La Rioja (UR)-Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Brock University [Canada], Institut Oenologique de Champagne (IOC), Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Kemp, Belinda [0000-0002-4333-6909]

Subjects
Synthetic grape juice, [SDV]Life Sciences [q-bio], food and beverages, Proteins, Wine, Saccharomyces cerevisiae, Proteases, Foam, Article, saccharomyces cerevisiae, lcsh:QD241-441, Botrytis cinerea, lcsh:Organic chemistry, botrytis cinerea, Polysaccharides, Alcohols, Fermentation, Electrophoresis, Polyacrylamide Gel, Vitis, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Botrytis, Alcoholic fermentation
Abstract

Botrytis cinerea is a fungal pathogen responsible for the decrease in foamability of sparkling wines. The proteolysis of must proteins originating from botrytized grapes is well known, but far less information is available concerning the effect of grape juice contamination by Botrytis. The impact from Botrytis on the biochemical and physico-chemical characteristics of proteins released from Saccharomyces during alcoholic fermentation remains elusive. To address this lack of knowledge, a model grape juice was inoculated with three enological yeasts with or without the Botrytis culture supernatant. Size exclusion chromatography coupled to multi-angle light scattering (SEC-MALLS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) techniques (AgNO3 and periodic acid Schiff staining) was used in the study. When Botrytis enzymes were present, a significant degradation of the higher and medium MW molecules released by Saccharomyces was observed during alcoholic fermentation whilst the lower MW fraction increased. For the three yeast strains studied, the results clearly showed a strong decrease in the wine foamability when synthetic musts were inoculated with 5% (v/v) of Botrytis culture due to fungus proteases

Published
2020

32. Functional role of [epsilon]-tubulin in the assembly of the centriolar microtubule scaffold

Author

Dupuis-Williams, Pascale, Fleury-Aubusson, Anne, de Loubresse, Nicole Garreau, Geoffroy, Helene, Vayssie, Laurence, Galvani, Angelique, Espigat, Aude, and Rossier, Jean

Subjects
Tubulins, Microtubules -- Physiological aspects, Biological sciences
Abstract

Centrioles and basal bodies fascinate by their spectacular architecture, featuring an arrangement of nine microtubule triplets into an axial symmetry, whose biogenesis relies on yet elusive mechanisms. However, the recent discovery of new tubulins, such as [delta]-, [epsilon]-, or [eta]-tubulin, could constitute a breakthrough for deciphering the assembly steps of this unconventional microtubule scaffold. Here, we report the functional analysis in vivo of [epsilon]-tubulin, based on gene silencing in Paramecium, which demonstrates that this protein, which localizes at the basal bodies, is essential for the assembly and anchorage of the centriolar microtubules.

Published
2002

40. Foamability of bentonite treated wines : Impact of new Acacia gum fractions obtained by Ionic Exchange Chromatography (IEC)

Author

Apolinar Valiente, Rafael, Williams, Pascale, Salmon, Thomas, Nigen, Michael, Sanchez, Christian, Marchal, Richard, Doco, Thierry, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Laboratoire d'Oenologie et Chimie Appliquée, Université de Reims Champagne-Ardenne (URCA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Oenologie et de Chimie Appliquée, Unité de Recherche Vigne et Vins de Champagne Stress et Environnement - EA 4707 (URVVC), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
hydrophobic chromatography, oenologie, chromatographie hydrophobe, bentonite, [SDV]Life Sciences [q-bio], denver mud, vin, ionic chromatography exchange, sparkling wine, foamibility, wine, acacia senegal gum
Abstract

Foamability of bentonite treated wines : Impact of new Acacia gum fractions obtained by Ionic Exchange Chromatography (IEC). ). ŒNOIVAS 2019 - 11. International Symposium of Œnology, Bordeaux- 11. edition In Vino Analytica Scientia

Published
2019

41. Proteins that control the geometry of ciliary ends

Author

Louka, Panagiota, Vasudevan, Krishna Kumar, Mayukh Guha, Joachimiak, Ewa, Wloga, Dorota, Tomasi, Raphaël F.‑X., Baroud, Charles N., Dupuis‑Williams, Pascale, Galati, Domenico F., Pearson, Chad G., Rice, Luke M., Moresco, James J., Yu‑Yang Jiang, Lechtreck, Karl, Dentler, William, Gaertig, Jacek, and Yates, John R.

Subjects
Cell Biology, Molecular Biology
Abstract

We studied three conserved ciliary proteins, FAP256/CEP104, CHE12/Crescerin and ARMC9, in the ciliate Tetrahymena. Using a combination of super-resolution structural illumination and electron microscopy we show that these proteins localize near the plus ends of ciliary microtubules where they control their length and subsequently the structural organization of ciliary ends. Our results show that defects in the geometry of ciliary ends affect the function of cilia.

Published
2019
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44. The Haemophilus influenzae sxy-1 mutation is in a newly identified gene essential for competence

Author

Williams, Pascale M., Bannister, Laura A., and Redfield, Rosemary J.

Subjects
Hemophilus influenzae -- Research, Microbial mutation -- Analysis, Transposons -- Analysis, Biological sciences
Abstract

Mapping and sequencing enable the identification of the sxy-1 mutation of Haemophilus influenzae as a G-to-A transition in an open reading frame of the rec-1 gene in a small DNA fragment. Abolition of both DNA uptake and transformation by a transposon insertion in Sxy reveals that sxy encodes a function necessary for competence. Sxy functions as a positive regulator of competence and the presence of a multicopy plasmid with wild-type Sxy gene gives constitutive competence to wild-type cells.

Published
1994

45. The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence

Author

Dorocicz, Irene R., Williams, Pascale M., and Redfield, Rosemary J.

Subjects
Adenylate cyclase -- Genetic aspects, Hemophilus influenzae -- Genetic aspects, Cloning -- Analysis, Gene expression -- Analysis, Biological sciences
Abstract

Acquisition of nutrients is part of the DNA uptake process, and the cAMP-dependent catabolite regulatory protein (CRP), with cyclic AMP (cAMP), governs the use and absorption of nutrients in the strain of Haemophilus influenzae. Exogenous cAMP induces competence in the H. influenzae adenylate cyclase gene (cya), demonstrating similarity to its wild-type parent. Models where induced competence occurs require higher intracellular cAMP levels and an unknown regulatory event. Ribose or xylose do not undergo fermentation in the presence of H. influenzae strains CRP or cya.

Published
1993

49. The impact of distillation process on prebiotic activity of different oligosaccharidic fractions extracted from grape seeds

Author

Bordiga, Matteo, Meudec, Emmanuelle, Williams, Pascale, Doco, Thierry, Montella, Rosa, Coïsson, Jean Daniel, Travaglia, Fabiano, Dipartimento di Scienze del Farmaco and Drug and Food Biotechnology Center, Università degli Studi del Piemonte Orientale - Amedeo Avogadro (UPO), Sciences Pour l'Oenologie (SPO), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Proge Farm S.R.L., Università degli Studi del Piemonte Orientale-Amedeo Avogadro (ITALY), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Vegetal Biology, oenologie, education, food and beverages, pépin de raisin, oligosaccharide, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Biologie végétale, Sciences agricoles, health care economics and organizations, Agricultural sciences
Abstract

The impact of distillation process on prebiotic activity of different oligosaccharidic fractions extracted from grape seeds.. 7. International Symposium on Macromolecules and Secondary Metabolites of Grapevine and Wine, MACROWINE2018

Published
2018

50. Are polysaccharides important to modulate protein/tannin interactions ?

Author

Doco, Thierry, Brandão, Elsa, Santos Silva, Mafalda, García-Estévez, Ignacio, Williams, Pascale, Mateus, Nuno, de Freitas, Victor, Soares, Susana, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), REQUIMTE/LAQV, Faculdade de Ciências, Universidade do Porto, Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Universidade do Porto [Porto], and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Vegetal Biology, tanin, education, food and beverages, tannin, Agricultural sciences, protéine, polysaccharide, vin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, wine, protein, health care economics and organizations, Biologie végétale, Sciences agricoles
Abstract

Are polysaccharides important to modulate protein/tannin interactions ? . 29. International Conference on Polyphenols and 9th Tannin Conference

Published
2018

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