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2. Effect of brassinosteroids on ammonium uptake via regulation of ammonium transporter and N-metabolism genes in Arabidopsis

Author

B. T. Zhao, X. F. Zhu, J. H. Jung, and Y. H. Xuan

Subjects
brassinosteroid signaling transcription factor, glutamate synthase, glutamine synthetase, mutant, Biology (General), QH301-705.5, Plant ecology, QK900-989
Abstract

Several studies have been performed to elucidate the role of brassinosteroids (BRs) in plant growth and development. However, information on the role of BR signaling in nutrient uptake is limited. This study explores the relationship between BRs and ammonium transporter 1 (AMT1) expression in Arabidopsis roots. We found that BR treatment reduced the expression of AMT1 genes and that a BR receptor BRI1 mutant bri1-5 reversed its BR-repressed expression. Furthermore, the BR signaling transcription factor, BES1, regulates AMT1 expression in roots. NH4 +-mediated repression of AMT1;1, AMT1;2, and AMT1;3 was suppressed in a gain-of-function BES1 mutant (bes1-D). This mutant was more sensitive to methyl-ammonium and contained a higher ammonium content compared to wild-type plants. However, BES1 failed to bind E-box elements present in the promoter region of the AMT1 genes. Furthermore, NH4 +-mediated glutamine synthetase (GS) and glutamine oxoglutarate aminotransferase (GOGAT) gene expressions were partially inhibited, and GS activity was slightly lower in the bes1-D mutant relative to that observed in wild-type En2 roots. NH4 +-mediated AMT1 suppressions are known to be caused by N-metabolites rather than NH4 + itself, and glutamine application inhibited AMT1 expression in both En2 and bes1-D indicating that BES1 activation inhibited NH4 +-mediated GS/GOGAT induction, which might in turn inhibit AMT1 repression. In conclusion, the present study demonstrates that BR regulated nitrogen uptake and assimilation via the BR signaling pathway.

Published
2016
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3. [Clinical features and advances in diagnoses and treatment of dyskeratosis congenita]

Author

Z Q, Yin, Y, Wan, and X F, Zhu

Subjects
Humans, Dyskeratosis Congenita
Abstract

先天性角化不良（DC）是以皮肤黏膜异常、进行性骨髓衰竭和高恶性肿瘤风险为特征的短端粒综合征，发病率低、临床异质性高、起病隐匿，易被误诊、漏诊。DC的诊断主要依靠患者临床表现、家族史、端粒长度及基因检测。目前临床上DC相关骨髓衰竭的主要治疗手段为口服雄激素及造血干细胞移植。现从DC的临床特点及治疗手段研究进展进行综述。.

Published
2022

5. [Advances in bone marrow failure related ribosomopathies]

Author

J, Li, Y, Wan, and X F, Zhu

Subjects
Anemia, Aplastic, Humans, Bone Marrow Failure Disorders, Bone Marrow Diseases, Anemia, Diamond-Blackfan
Abstract

由机体核糖体合成或功能障碍所导致的一组疾病称为核糖体病，多数为遗传性罕见病。其中，以造血衰竭为主要表现的核糖体病主要包括先天性纯红细胞再生障碍性贫血（Diamond-Blackfan anemia，DBA）、舒-戴综合征（Shwachma-Diamond syndrome，SDS）以及部分先天性角化不良（congenital dyskeratosis，DC）。由于该类疾病发病率低，临床异质性大，我国医师对其认识不够充分，此类疾病常被误诊、漏诊。本文从发病机制、临床特点及诊疗手段的角度，对核糖体异常相关造血衰竭疾病进行综述。.

Published
2021

8. [Familial platelet disorder with predisposition to myeloid leukemia (FPD/AML): a case report and literature review]

Author

R R, Zhang, X J, Chen, Y Y, Ren, W Y, Yang, and X F, Zhu

Subjects
Male, 家族性血小板疾病并急性髓系白血病倾向, Gene, RUNX1, 胚系突变, Pedigree, 论著, Leukemia, Myeloid, Acute, Blood Coagulation Disorders, Inherited, Child, Preschool, Core Binding Factor Alpha 2 Subunit, Mutation, Germline mutation, Familial platelet disorder with predisposition to myeloid leukemia, Humans, Genetic Predisposition to Disease, 基因，RUNX1, Blood Platelet Disorders, Child
Abstract

目的 探讨RUNX1胚系突变导致的家族性血小板疾病并急性髓系白血病倾向（FPD/AML）患儿及其家族成员的临床特点及基因突变情况。 方法 对2019年10月中国医学科学院血液病医院儿童血液诊疗中心收治的1例FPD/AML患儿及部分家族成员的临床资料及基因突变结果进行分析。并以“RUNX1胚系突变”“家族性血小板疾病并急性髓系白血病倾向”“RUNX1 germline mutation”“FPD/AML”为检索词，检索建库至2020年9月中文数据库（中国知网数据库、万方数据库及维普数据库）及PubMed数据库进行文献复习。 结果 患儿为5岁男孩，因发现血小板减少3年入院。体格检查提示存在皮肤出血点，其他无明显异常。辅助检查：外周血常规示WBC 6.38×109/L，HGB 113 g/L，PLT 54×109/L，中性粒细胞绝对计数4.03×109/L，血小板平均体积（MPV）9.1 fl。骨髓涂片提示巨核系发育异常。涂片免疫CD42b及CD41酶标提示存在小巨核细胞。基因检测提示RUNX1（exon3: c.520delC:p.R174Efs*10, NM_001001890）的移码突变，经口腔上皮细胞验证为胚系突变。家族史中共有5名家族成员存在血液系统疾病并相继死亡。患儿母亲及外祖父先后进行了与血液肿瘤疾病相关的137个基因热点区域的基因检测，均检测到与患儿相同位点的RUNX1移码突变，但是三人的临床症状十分不同。文献检索共检索到相关英文文献37篇，报道了70多个FPD/AML家族，未检索到相关中文文献。 结论 RUNX1胚系突变是导致FPD/AML的病因，进展为髓系恶性肿瘤的风险极高，携带相同突变的家族成员可能表现出非常不同的临床症状和严重程度。

Published
2021

9. [Safety and feasibility of laparoscopic double-flap technique in digestive tract reconstruction after proximal gastrectomy for esophagogastric junction tumors larger than 5 cm]

Author

X F, Zhu, W W, Xiong, Y S, Zheng, L J, Luo, J, Li, H P, Huang, Z S, Fan, Y L, Xue, S J, Luo, Y T, Xu, J, Wan, and W, Wang

Subjects
Adult, Male, Leiomyoma, Gastrointestinal Stromal Tumors, Anastomosis, Surgical, Stomach, Middle Aged, Surgical Flaps, Esophagus, Treatment Outcome, Gastrectomy, Stomach Neoplasms, Feasibility Studies, Humans, Female, Laparoscopy, Esophagogastric Junction, Retrospective Studies
Published
2021

10. [Efficacy and prognostic factors of the chemotherapy regimen of CCLG-ALL-2008 on pediatric acute lymphoblastic leukemia with ETV6-RUNX1 rearrangement]

Author

F, Liu, X J, Chen, Y, Guo, W Y, Yang, X, Chen, X Y, Zhang, R R, Zhang, Y Y, Ren, and X F, Zhu

Subjects
Male, 儿童, Pediatric, ETV6-RUNX1 rearrangement, Oncogene Proteins, Fusion, CCLG-ALL-2008方案, Leukemia, lymphoblastic, acute, Precursor Cell Lymphoblastic Leukemia-Lymphoma, 白血病，淋巴细胞，急性, 预后, Prognosis, Disease-Free Survival, 论著, ETV6-RUNX1融合基因, CCLG-ALL-2008 protocol, Core Binding Factor Alpha 2 Subunit, Humans, Female, Child, Retrospective Studies
Abstract

目的 分析CCLG-ALL-2008方案治疗ETV6-RUNX1融合基因阳性儿童急性淋巴细胞白血病（ALL）的长期疗效及预后相关因素。 方法 对2008年4月至2015年4月期间于中国医学科学院血液病医院儿童血液病诊疗中心接受CCLG-ALL-2008方案治疗的178例初诊ETV6-RUNX1阳性ALL患儿进行回顾性分析。 结果 全部178例患儿中男100例，女78例，中位年龄4（1~13）岁，初诊中位WBC为9.46（1.25~239.83）×109/L。中位随访时间为73.5（1~124）个月，3例在诱导化疗阶段死亡，1例于诱导化疗后失访，1个疗程诱导化疗完全缓解率为97.8%（174/178）。178例患儿中24例复发，累积复发率为15.9%，中位复发时间为35.5（1~62）个月，单纯骨髓复发占83.3%（20/24），晚期复发占79.2%（19/24），早期复发5例中1例存活，晚期复发19例中7例存活。ETV6-RUNX1阳性患儿的5年总生存（OS）率、无事件生存（EFS）率分别为（89.4±2.4）%、（82.1±6.9）%，预期10年OS率、EFS率分别为（88.6±2.5）%、（77.3±4.0）%。分析预后相关因素，单因素分析结果显示初诊中枢神经系统（CNS）2状态、泼尼松预治疗反应不佳、高危组、诱导化疗第15天骨髓流式微小残留病（MRD）≥5%、第33天ETV6-RUNX1融合基因未转阴、第12周骨髓流式MRD≥1×10−4以及第12周ETV6-RUNX1融合基因未转阴为生存相关预后不良因素。Cox模型多因素分析显示，诱导化疗第15天骨髓流式MRD≥5%（P=0.033）、第12周ETV6-RUNX1融合基因未转阴（P=0.045）为影响OS的独立预后不良因素；而初诊CNS2状态（P=0.033）及第12周骨髓流式MRD≥1×10−4（P=0.010）则为影响EFS的独立预后不良因素。 结论 CCLG-ALL-2008方案治疗ETV6-RUNX1阳性ALL患儿总体预后良好，初诊CNS2状态、诱导化疗第15天骨髓流式MRD≥5%、第12周ETV6-RUNX1未转阴及MRD≥1×10−4者需要尽早强烈干预治疗。

Published
2020

12. [Research advances in aberrant DNA methylation in juvenile myelomonocytic leukemia]

Author

W R, Liang, W Y, Yang, and X F, Zhu

Subjects
Leukemia, Myelomonocytic, Juvenile, Humans, CpG Islands, DNA Methylation
Abstract

幼年型粒单核细胞白血病（JMML）是一类诊治较为困难的儿童造血系统恶性疾病，近年来研究发现基因启动子异常DNA甲基化在JMML的发病机制中占据重要地位，并与临床不良预后显著相关。全基因组甲基化水平分析证实了DNA甲基化与疾病不同临床亚型的相关性，对临床结局具有预测作用。部分临床案例和体外研究表明，去甲基化药物对JMML的治疗有一定效果。本文对异常DNA甲基化在JMML中的研究进展进行综述。.

Published
2020

13. [Effect of imatinib on the height of children with chronic myeloid leukemia in the chronic phase]

Author

F Y, Zheng, Yanli, Zhang, L Q, Zhang, B C, Liu, L, Meng, J, Jin, H L, Liu, Z M, Sun, L E, Lin, P C, Lei, X F, Zhu, H X, Ma, Z S, Lu, H, Jiang, Y H, Zhao, H, Lin, X, Zhang, G P, Yang, H L, Zhu, S N, Chen, Y, You, W M, Li, Q X, Bai, X L, Zhao, Z Y, Li, X M, Shen, L P, Zhang, and Q, Jiang

Subjects
Adult, Male, 儿童, China, 身高, Time Factors, Adolescent, Antineoplastic Agents, 慢性期, Leukemia, myeloid, chronic, Young Adult, 白血病，髓系，慢性, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Humans, 伊马替尼, Child, Children, Chronic-phase, Height, Infant, 论著, Treatment Outcome, Child, Preschool, Imatinib, Imatinib Mesylate, Female
Abstract

目的 评估伊马替尼对慢性髓性白血病慢性期（CML-CP）儿童身高的影响。 方法 2018年7月至2019年7月，在全国范围内对诊断时年龄0.05）。多因素分析显示，服药初始年龄较小（偏回归系数为0.122，B＝0.572，t＝10.733，P

Published
2020

15. [Clinical outcome of CAG regimen in 26 children with aucte myeloid leukemia]

Author

X, Chen, M, Ruan, F, Liu, T F, Liu, X J, Chen, Y, Guo, L, Zhang, W Y, Yang, L X, Chang, B B, Zhao, and X F, Zhu

Subjects
Leukemia, Myeloid, Acute, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols, Granulocyte Colony-Stimulating Factor, Remission Induction, Cytarabine, 短篇论著, Humans, Aclarubicin, Child
Published
2020

17. Diversity and transition characteristics of sticking and non-sticking periodic impact motions of periodically forced impact systems with large dissipation

Author

Y. Q. Shi, Xiaohong Lv, G. W. Luo, X. F. Zhu, and S. S. Du

Subjects
Physics, Applied Mathematics, Mechanical Engineering, Aerospace Engineering, Ocean Engineering, Mechanics, Dissipation, Collision, 01 natural sciences, 010305 fluids & plasmas, Periodic function, Vibration, Discontinuity (linguistics), Control and Systems Engineering, 0103 physical sciences, Piecewise, Electrical and Electronic Engineering, 010301 acoustics, Bifurcation, Incidence (geometry)
Abstract

A forced vibration system with dual bodies and an endstop is considered in this paper. A large energy loss is considered as one of the mass blocks of the system hits the endstop, and the impact associated with the large energy loss is first assumed to be completely plastic. The plastic impact may bring about the occurrence of the sticking phase, which is equivalent to a change in the structure of the forced vibration system at a certain stage after the impact. The incidence relation between dynamical characteristics and model parameters is studied through the multi-target and multi-parameter collaborative simulation analysis for determining the reasonable matching range of parameters. Pattern types, occurrence regions, distribution regularities and bifurcation characteristics of periodic and subharmonic impact vibrations are presented on a series of parameter planes. The key features of Poincare mapping, associated with the plastic impacts, are primarily manifested in piecewise continuity caused by sliding bifurcation and grazing discontinuity induced by grazing bifurcation. Integrative effects of these two nonstandard bifurcations can bring about some abnormal transitions to occur. The large dissipation case associated with small collision recovery coefficient is briefly analyzed, and the induced mechanism of chatter-sticking motion and the incidence relation between the sticking characteristics and the restitution coefficient R are discussed. The nonstandard dynamic characteristics associated with the plastic impacts are further demonstrated by dynamic mechanical behaviors of two practical impact machines applied in engineering.

Published
2018
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18. Carbon-assisted growth of SiOx nanowires

Author

S.-H. Li, X.-F. Zhu, and Y.-P. Zhao

Subjects
Carbon -- Thermal properties, Argon -- Thermal properties, Silicon compounds -- Thermal properties, Chemicals, plastics and rubber industries
Abstract

A systematic investigation of how the growth conditions, such as the growth temperature, the oxygen-to-Ar carrier gas ratio, and growth time, affect the formation of SiOx nanowires has been performed. Higher growth temperature and appropriate low oxygen gas flow helped to promote long nanowire growth.

Published
2004

19. [Outcome of children with T cell acute lymphoblastic leukemia treated with Chinese Children Leukemia Group acute lymphoblastic leukemia (CCLG-ALL) 2008 protocol]

Author

X M, Liu, X J, Chen, Y, Zou, S C, Wang, M, Wang, L, Zhang, Y M, Chen, W Y, Yang, Y, Guo, and X F, Zhu

Subjects
Male, Oncogene Proteins, Fusion, Antineoplastic Agents, Kaplan-Meier Estimate, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma, Prognosis, Disease-Free Survival, Survival Rate, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols, Humans, Female, Child, Retrospective Studies
Published
2019

20. [A correlation study between the minimal residual disease detection using multiparameter flow cytometry and prognosis of childhood acute B lymphoblastic leukemia]

Author

J, Feng, X J, Chen, X M, Liu, Y, Zou, Y, Guo, W Y, Yang, Y M, Chen, L, Zhang, S C, Wang, M, Ruan, F, Liu, T F, Liu, B Q, Qi, X F, Zhu, and H J, Wang

Subjects
Neoplasm, Residual, 短篇论著, Humans, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Child, Correlation of Data, Flow Cytometry, Prognosis, Immunophenotyping
Published
2019

21. [Two cases of adult-onset neuronal intranuclear inclusion disease diagnosed by skin biopsy]

Author

Q, Tong, K J, Ju, X F, Zhu, X Y, Tian, J L, Zheng, and L J, Xue

Subjects
Adult, Biopsy, Intranuclear Inclusion Bodies, Humans, Neurodegenerative Diseases, Age of Onset, Skin
Abstract

分析两例成年起病的神经元核内包涵体病（NIID）的临床、影像、皮肤病理特征。两例患者均为老年起病的NIID，但两例的起病方式不同，一例以反复发作性意识障碍为表现急性起病，另外一例以无力和认知功能障碍为表现慢性起病。两例均行相关基因检测排除脆性X相关震颤/共济失调综合征（FXTAS）。NIID临床表现多样，易被误诊，急性起病者易被误诊为脑炎、癫痫或急性缺血性脑血管病，慢性起病者易被误诊为白质脑病或脑小血管病。.

Published
2019

22. [Clinical and molecular characteristics of GATA2 related pediatric primary myelodysplastic syndrome]

Author

W B, An, C, Liu, Y, Wan, X Y, Chen, Y, Guo, X J, Chen, W Y, Yang, Y M, Chen, Y C, Zhang, and X F, Zhu

Subjects
儿童, Pediatric, Adolescent, Germline, Hematopoietic Stem Cell Transplantation, Infant, 骨髓增生异常综合征, GATA2 Transcription Factor, 论著, Leukemia, Myeloid, Acute, GATA2 mutation, Child, Preschool, Myelodysplastic Syndromes, GATA2, Humans, Child, Myelodysplastic syndrome, Germ-Line Mutation
Abstract

目的 探讨我国GATA2突变相关儿童原发性骨髓增生异常综合征（MDS）的发生情况、临床特点及分子生物学特征。 方法 回顾性分析2007年1月至2018年1月129例儿童原发性MDS患者临床资料，采用二代测序技术检测GATA2及髓系恶性肿瘤相关基因突变情况。分析基因突变谱及其与临床表现型的关系。 结果 在所有129例患者中，11例（8.5%）检出GATA2胚系突变。在50例MDS伴原始细胞增高（MDS-EB）和急性髓系白血病伴MDS相关改变（AML-MRC）患者中，GATA2胚系突变占14.0%。GATA2突变多位于第二个锌指（ZF2）区。多因素分析结果显示，SETBP1体细胞突变（P=0.041，OR=9.003，95%CI 1.098~73.787）和独立的7号染色体单体（P=0.002，OR=24.835，95%CI 3.305~186.620）与GATA2胚系突变显著相关。与GATA2野生型的患者相比，GATA2突变型患者中位发病年龄更大[8（1~16）岁对6岁（1月龄~18岁），P=0.035]，更易伴有7号染色体单体（72.7%对5.2%，P

Published
2019

23. EFFICACY OF PENICILLIUM CHRYSOGENUM STRAIN SNEF1216 AGAINST ROOT-KNOT NEMATODES (MELOIDOGYNE INCOGNITA) IN CUCUMBER (CUCUMIS SATIVUS L.) UNDER GREENHOUSE CONDITIONS

Author

H. Y. Fan, X. Y. Liu, A. Javeed, Y. H. Xuan, A. Sikandar, M. F. Iqbal, M. Ahmed, X. F. Zhu, M. Y. Zhang, Y. X. Duan, Y. Y. Wang, and L. J. Chen

Subjects
biology, business.industry, Pest control, Biological pest control, Greenhouse, Penicillium chrysogenum, biology.organism_classification, Horticulture, Germination, Meloidogyne incognita, Natural enemies, business, Agronomy and Crop Science, Cucumis, Ecology, Evolution, Behavior and Systematics
Published
2019
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24. [A long-term follow-up study of 82 children with acute myeloid leukemia]

Author

M, Ruan, B Q, Qi, F, Liu, T F, Liu, X M, Liu, X J, Chen, W Y, Yang, Y, Guo, L, Zhang, Y, Zou, Y M, Chen, and X F, Zhu

Subjects
Male, Leukemia, Myeloid, Acute, Adolescent, Child, Preschool, Antineoplastic Combined Chemotherapy Protocols, Remission Induction, Humans, Infant, Female, Child, Prognosis, Follow-Up Studies, Retrospective Studies
Published
2018

27. ['3-step' strategy of transperineal anastomotic urethroplasty for the simple pelvic fracture urethral distraction defect in male patients]

Author

J W, Wang, L B, Man, G L, Huang, H, Wang, X, Xu, X F, Zhu, W, Li, and Z H, Liu

Subjects
Adult, Male, Urethral Stricture, Treatment Outcome, Urologic Surgical Procedures, Male, Urethra, Anastomosis, Surgical, Humans, Pelvic Bones, Pelvis, Retrospective Studies
Abstract

To evaluate the clinical effect of "3-step" strategy of transperineal anastomotic urethroplasty for the simple pelvic fracture urethral distraction defect in male patients.We retrospectively reviewed the clinical data of 162 male patients with simple traumatic posterior urethral stricture or stenosis admitted from January 2014 to October 2015. All had no complex complications, such as urethroperineal fistulas or urethrorectal fistulas. Before referral to Department of Urology, Beijing Jishuitan Hospital, 64 patients had undergone previous treatments: urethroplasty in 30 patients (18.5%), early urethral realignment in 17 patients (10.5%) and 17 patients (10.5%) who had undergone internal urethrotomy. The remaining 98 patients received the suprapubic cystostomy in the acute setting. All of them had received transperineal anastomotic urethroplasty with "3-step" strategy. Step 1, the bulbar urethra was circumferentially mobilized and tension-free anastomosis could be performed after the scar was completely incised and removed. Step 2, if after step 1 a tension-free anastomosis could not be achieved, were routed the distal urethra between the separated corporal bodies. Step 3, if the anastomosis still seemed to be under tension, we could perform pubectomy, partial or total removal, to get a better exposure of the apex of the prostate-membranous urethra.The mean age of the patients included in this study was 36.3 years (rangingfrom 16-74 years). The mean time between incidents and operation was 13.5 months (ranging from 3-124 months) and the mean length of stricture was 2.7 cm (ranging from 0.5-6.5 cm).The mean time of operation was 92 (45-240) min and the mean evaluated blood lose was 120 (60-800) mL. Three patients (1.9%) received blood transfusing during or after the operations. The numbers of the patients who completed step 1, step 2 and step 3 were 50(30.9%), 74(45.7%) and 38(23.5%), respectively. There were 4 (2.5%) patients who needed the combined transpubic and transperineal approach for tension-free anastomosis after removing an entire wedge of anterior pubis. The mean follow-up was 19.5 months and 18 patients' strictures recurred with manifestation of decreased stream of dysuria. The overall success rate was 88.9%(144/162).Based on the "3-step" strategy of transperineal anastomotic urethroplasty, patients with simple PFUDD can achieve a tension-free anastomosis. The present clinical data showed a successful rate of 88.9% (144/162).

Published
2018

28. [Pediatric myeloid neoplasms associated with eosinophilia and platelet-derived growth factor receptor beta gene rearrangement: a case report and literature review]

Author

X Y, Zhang, T F, Liu, C W, Li, Q H, Li, and X F, Zhu

Subjects
Gene Rearrangement, Male, China, Myeloproliferative Disorders, Remission Induction, Infant, Translocation, Genetic, Receptor, Platelet-Derived Growth Factor beta, Karyotyping, Neoplasms, Eosinophilia, Imatinib Mesylate, Humans, Child, In Situ Hybridization, Fluorescence
Published
2018

29. SCC behaviours of austenitic stainless steel Z3CN20-09M in high temperature water

Author

Yonghao Lu, Z. R. Chen, X. F. Zhu, and Tetsuo Shoji

Subjects
Austenite, Phase boundary, Materials science, Mechanical Engineering, Metallurgy, engineering.material, Intergranular corrosion, Condensed Matter Physics, Corrosion, Mechanics of Materials, Ferrite (iron), engineering, Pitting corrosion, General Materials Science, Austenitic stainless steel, Stress corrosion cracking
Abstract

Stress corrosion cracking (SCC) behaviours of Z3CN20-09M stainless steel in high temperature water containing Cl− were studied. The results indicated that SCC sensitivity was inconsistent with test temperature. The minimum and maximum of SCC sensitivity occurred at 320 and 290°C respectively, and SCC sensitivity at 250°C fell between them. SCC crack initiated preferentially at bottom of corrosion pit or along phase boundary between austenite and ferrite, and its propagation depended on relative orientation to the phase boundary. SCC crack parallel to the phase boundary propagated along the phase boundary, while that perpendicular to the phase boundary was hindered to propagate.

Published
2014
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31. PF233 CONSISTENCY OF GENOMIC ALTERATIONS ASSESSED BY NEXT-GENERATION SEQUENCING AMONG CIRCULATING TUMOR DNA, BONE MARROW AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN PEDIATRIC AML

Author

Y. Sun, C. Liu, F. Lou, X.-F. Zhu, Y. Guo, L.-P. Liu, A.-L. Zhang, S.-C. Wang, L.-X. Chang, X.-M. Liu, B.-Q. Qi, Y. Zou, M. Ruan, Y.-M. Chen, S. Cao, L. Zhang, Y.-C. Zhang, F. Liu, X.-J. Chen, and X.-Y. Chen

Subjects
medicine.anatomical_structure, Circulating tumor DNA, Consistency (statistics), business.industry, Cancer research, medicine, Hematology, Bone marrow, business, Peripheral blood mononuclear cell, Pediatric AML, DNA sequencing
Published
2019
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32. EFFICACY OF SNEB183 (SINORHIZOBIUM FREDII) AGAINST SOYBEAN CYST NEMATODE (HETERODERA GLYCINES) UNDER FIELD AND GROWTH CHAMBER CONDITIONS.

Author

Y. Y., WANG, R. H., YUAN, X. L., LIAN, A., SIKANDAR, X. F., ZHU, X. Y., LIU, H. Y., FAN, L. J., CHEN, and Y. X., DUAN

Subjects
SOYBEAN cyst nematode, PLANT biomass, PEST control, BIOLOGICAL pest control agents, GERMINATION, GLYCINE
Abstract

Soybean cyst nematode (Heterodera glycines) has become a serious risk for soybean production globally. Chemical nematicides pose a severe threat to human health and also pollute the environment whereas, biological control is a safe and eco-friendly method used to control pathogens. The aim of this study was to discover novel biocontrol agents against H. glycines by coating soybean seeds with biocontrol agent Sneb183 (Sinorhizobium fredii) and to assess its potential to control this pest. The stability and efficiency of Sneb183 (S. fredii) to control H. glycines in field and growth chamber experiments were investigated. Seed coating with Sneb183 significantly decreased the presence of H. glycines by 35.58%, 64.09% and 50.43% in experiments J2 (second-stage juveniles), J3 (third-stage juveniles) and J4 (fourthstage juveniles), respectively as compared to control. A progressive increase in the concentrations of the Snef183 resulted in a significant reduction of H. glycines. Additionally, Sneb183 boosted seed germination and seed vigor index. Moreover, seed coating with Sneb183 increased plant biomass. These results demonstrate that Sneb183 (S. fredii) is a promising biocontrol agent against H. glycine and biomass promoter of soybean. [ABSTRACT FROM AUTHOR]

Published
2021
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33. Effect of phase boundary on nucleation and propagation of microcrack in nuclear grade Z3CN20-09M stainless steel

Author

X. F. Zhu and Yonghao Lu

Subjects
Austenite, Phase boundary, Materials science, Scanning electron microscope, Mechanical Engineering, Metallurgy, Lüders band, Nucleation, Fractography, Condensed Matter Physics, Physics::Geophysics, Condensed Matter::Materials Science, Mechanics of Materials, Ferrite (iron), General Materials Science, Deformation (engineering), Composite material
Abstract

In order to study the effect of the phase boundary between austenite and ferrite phases on the nucleation and propagation behaviours of microcracks in nuclear grade Z3CN20-09M stainless steel, an in situ scanning electron microscopy (SEM) tensile technique was used to study the deformation, nucleation and propagation of microcracks during tension. The fractography was subsequently observed by SEM. The results indicated that, during tension, the deformation first occurred in the austenitic grains followed by in the ferrite, and the microcracks were subsequently preferential formed at the phase boundary between austenite and ferrite phases. In the case of the phase boundary perpendicular to the loading direction, microcracks nucleated and subsequently propagated along the phase boundary. In the case of the phase boundary parallel to the loading direction, microcacks nucleated at the intersection of slip bands and the phase boundary and propagated perpendicularly to the phase boundary, resulting in d...

Published
2013
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35. [Analysis of clinical characteristics and prognosis of non-severe aplastic anemia children with chromosomal abnormalities]

Author

S, Zhu, W B, An, Y, Wan, Y Y, Ren, R R, Zhang, J L, Zhang, C, Liu, C C, Sun, L X, Chang, X J, Chen, W Y, Yang, Y, Guo, Y M, Chen, Y, Zou, and X F, Zhu

Subjects
Chromosome Aberrations, Male, Adolescent, Anemia, Aplastic, Chromosome Disorders, Trisomy, Prognosis, Disease-Free Survival, Leukemia, Myeloid, Acute, Karyotyping, Myelodysplastic Syndromes, Disease Progression, Humans, Blood Transfusion, Child, Chromosomes, Human, Pair 8, Retrospective Studies
Published
2016

36. [Strategies in clinical diagnosis and treatment of steroid-resistant acute rejection after orthotopic liver transplantation]

Author

X J, Lu, Y H, Chen, Y, Ma, X F, Zhu, and X S, He

Subjects
Graft Rejection, Biopsy, Incidence, Antibodies, Monoclonal, Humans, Steroids, Antilymphocyte Serum, Liver Transplantation, Muromonab-CD3, Retrospective Studies
Abstract

To explore the diagnostic evidence and treatment strategies for steroid-resistant acute rejection (SRAR) after orthotopic liver transplantation.A retrospective analysis was performed among 1038 patients undergoing orthotopic liver transplantation in our hospital from January 2004 to December 2013. A total of 169 acute rejection (AR) episodes occurred in 153 patients. Sixteen of the patients were diagnosed with SRAR because of no response to large-dose steroid pulse therapy. The diagnosis and treatment of the 16 patients were analyzed retrospectively. Comparison of data was made by χ2 test or t test, and a P value of0.05 was considered to be significant.The incidence of AR after liver transplantation was 14.74% (153/1038) in all the patients. The incidence of SRAR was 9.47% (16/169) in patients with AR. In the 16 patients with SRAR, 3 were treated with anti-CD3 monoclonal antibody (OKT3), 9 were treated with monoclonal antibody against IL-2 receptor, and 4 received antithymocyte globulin (ATG) therapy. After treatment, SRAR was reversed in 12 of the 16 patients and caused death of the other 4 patients, yielding a reversal rate of 75% and a mortality rate of 25%.SRAR after liver transplantation has a low incidence rate but poor prognosis. The diagnosis of SRAR is mainly based on the clinical manifestation, laboratory test, liver biopsy, and poor response or rejection to methyl prednisolone pulse therapy. ATG and OKT3 achieve substantial outcomes in most of the patients in the treatment of SRAR. Particularly, compared with OKT3, ATG achieves a higher reversal rate and fewer adverse reactions, which is expected to become the first-line treatment of SRAR.

Published
2016

37. [Clinical and genetic features of dyskeratosis congenital with bone marrow failure in eight patients]

Author

Y, Wan, W B, An, J Y, Zhang, J L, Zhang, R R, Zhang, S, Zhu, L X, Chang, Y C, Zhang, F, Liu, W Y, Yang, X J, Chen, Y, Zou, Y M, Chen, and X F, Zhu

Subjects
Male, 儿童, Pancytopenia, 骨髓衰竭, DNA Mutational Analysis, Telomere-Binding Proteins, Infant, Nuclear Proteins, Cell Cycle Proteins, Exons, Telomere, Dyskeratosis Congenita, Bone marrow failure, 论著, DNA突变分析, Bone Marrow, Child, Preschool, 先天性角化不良, Mutation, Humans, 端粒, Female, Dyskeratosis congenital, Child, Telomerase
Abstract

To summary clinical and genetic features of childhood dyskeratosis congenital (DC) patients with bone marrow failure.The clinical data of 8 DC patients with bone marrow failure diagnosed between September 2010 and September 2015 were collected. Whole exons with flanking regions of the 16 telomere-related genes, including DKC1, TERC, TERT, NOP10, NHP2, TINF2 and so on, were analyzed by next generation sequence.Six males and two females were included, with a median age of 42(15-60) months. The median blood cell count at onset were as follow: WBC 3.99 (1.26-5.44) × 10(9)/L, ANC 1.11 (0.38-2.15) × 10(9)/L, RBC 2.45 (0.37-3.56) × 10(12)/L, HGB 82.5(15-127) g/L, PLT 27 (2-112) ×10(9)/L. Hypoplastic or marked hypoplastic bone marrow were seen in 6 patients. DKC1 mutiaton were indentified in 3 patients: one c.961CA mutation, and two c.1058CT mutation. TINF2 mutations were identified in 4 patients: c.849delC, c.844CT, c.811CT, c.862TA combined c.871delA. One patient had TINF2 mutation c.848CA combined TERT mutation c.1138CT. DKC1 c.961CA mutation, TINF2 c.849delC mutation and TINF2 c.871delA mutaion were not reported so far. 5 of 7 patients got better after androgen administration. During follow-up, one patient died of serious infection, the other seven patients continued the treatment.TINF2 and DKC1 mutations were the main genetic phenotypes in childhood DC with marrow failure patients. Androgen is effetive in some cases.

Published
2016

38. Biological activity of Co(III) and Ni(II) complexes of pyridine-2-carbaldehyde N(4)-methylthiosemicarbazone: Synthesis, characterization, crystal structure of Co(III) complex of pyridine-2-carbaldehyde N(4)-methylthiosemicarbazone1

Author

Q. Wang, X. F. Zhu, J. Zhou, Ming-Xue Li, Juan Zhao, C. L. Chen, and Y. H. Fan

Subjects
chemistry.chemical_classification, Stereochemistry, Ligand, Hydrogen bond, General Chemical Engineering, Imine, General Chemistry, Crystal structure, Medicinal chemistry, Coordination complex, Metal, chemistry.chemical_compound, chemistry, Transition metal, visual_art, Pyridine, visual_art.visual_art_medium
Abstract

Transition metal complexes formulated as [Co(L)2]ClO4 (I) and [Ni(L)2] · H2O (II), where HL = pyridine-2-carbaldehyde N(4)-methylthiosemicarbazone, have been synthesized. Complex I was characterized by elemental analysis, IR, MS and single-crystal X-ray diffraction studies. In complex I, the ligand is N2S tridentate, coordinating to the metal center through pyridine nitrogen, imine nitrogen and sulfur atoms. Hydrogen bonds link the different components to stabilize the crystal structure. Preliminary in vitro screening indicated that the free ligand was active against various bacteria and fungi and all the tested compounds showed significant antitumor activity against K562 leukaemia cell line, and can therefore be candidates for further stages of screening in vitro and/or in vivo.

Published
2012
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40. On corrosion behaviour of metal in biomass oil

Author

Y F Xu, Qiongjie Wang, X G Hu, L L Zhou, and X F Zhu

Subjects
Materials science, General Chemical Engineering, Metallurgy, Oxide, chemistry.chemical_element, Biomass, General Chemistry, Straw, Copper, Corrosion, Metal, chemistry.chemical_compound, chemistry, visual_art, visual_art.visual_art_medium, Hydroxide, General Materials Science, Pyrolysis
Abstract

Biomass oil obtained by the fast pyrolysis of straw is an acidic fuel with pH of 3·4–3·5. It contains a large amount of organic acids, phenol and water and, hence its application in varied industrial fields may result in the corrosion of different metals. In the present study, the corrosion performance of four kinds of iron, lead, steel and copper in biomass oil from the fast pyrolysis of straw was studied at different temperatures and for different test durations using a simulation corrosion evaluation apparatus for internal combustion engine fuel. The corroded metal surfaces were observed by optical micrography and analysed by X-ray photoelectron spectroscopy respectively. The experimental results showed that iron and lead were corroded seriously by the present biomass oil, while the corrosion extents of AISI 1045 steel and copper were significantly less. Layers of oxide and/or hydroxide were formed on these metal surfaces according to surface analysis. However, these layers were not protective ...

Published
2011
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41. Antitumor activity of manganese(II) and cobalt(III) complexes of 2-acetylpyridine schiff bases derived from S-methyldithiocarbazate: Synthesis, characterization, and crystal structure of the manganese(II) complex of 2-acetylpyridine S-methyldithiocarbazate

Author

C. L. Chen, X. F. Zhu, Ming-Xue Li, Niu Jishou, and H. M. Guo

Subjects
Schiff base, Ligand, Stereochemistry, General Chemical Engineering, chemistry.chemical_element, General Chemistry, Crystal structure, Manganese, chemistry.chemical_compound, Transition metal, chemistry, Pyridine, Polymer chemistry, Octahedral molecular geometry, 2-Acetylpyridine
Abstract

Transition metal complexes [Mn(L)2] (I) and [Co(L)2] · (ClO4) · H2O (II), where HL = 2-acetylpyridine S-methyldithiocarbazate, have been synthesized. Complex I was characterized by elemental analysis, IR spectra, and single-crystal X-ray diffraction studies. The manganese(II) atom in complex I adopts a distorted octahedral geometry with the Schiff base coordinated to it as a uninegatively charged tridentate chelating agent via the pyridine and azomethine nitrogen atoms and the thiolate sulfur atom. Biological studies carried out in vitro against K562 leukemia cancer cell line have shown that the free ligand and its metal complexes exhibited significant and different antitumor activity, since they exhibit IC50 values in the μM range.

Published
2011
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42. FTIR and Mass Spectral Analyses of an Upgraded Bio-oil

Author

L. Wu, X.-F. Zhu, Z.-L. Xia, L. Zhou, Z.-M. Zong, M.-J. Ding, Y. Zong, R.-L. Xie, S.-R. Tang, W. Zhao, and X.-Y. Wei

Subjects
Carbon disulfide, Renewable Energy, Sustainability and the Environment, Dispersity, Analytical chemistry, Energy Engineering and Power Technology, Mass spectrometry, Hexane, chemistry.chemical_compound, Fuel Technology, Nuclear Energy and Engineering, chemistry, Ion trap, Char, Benzene, Pyrolysis, Nuclear chemistry
Abstract

Bio-oil prepared from flash pyrolysis of rice stalk around 500°C was upgraded by filtration and catalytic esterification. Both raw and upgraded bio-oils were observed with an optical microscope. The upgraded bio-oil was extracted with benzene, carbon disulfide, hexane, and carbon tetrachloride, respectively, and the soluble fractions were analyzed with FTIR and GC/MS. In addition, ion trap mass spectrometer was used for analysis of the benzene-soluble fraction. The results show that the upgrading substantially improved the dispersity of organic droplets in the bio-oil and completely removed char particles from the bio-oil, but heavy species are still the main components in the upgraded bio-oil; the soluble fractions contain aliphatic, benzene-ring-containing, ester, and bonded hydroxyl moieties, and methoxy-containing species are the most abundant GC/MS-detectable compounds.

Published
2009
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43. Examination of the semen quality of patients with uraemia and renal transplant recipients in comparison with a control group

Author

N.-Q. Lu, L.-G. Xu, H.-M. Xu, L.-M. Jin, B. Xu, X.-F. Zhu, and Y. Wu

Subjects
Adult, Male, Infertility, medicine.medical_specialty, Adolescent, Cell Survival, Urology, media_common.quotation_subject, Fertility, Semen analysis, urologic and male genital diseases, Group B, Semen quality, Endocrinology, Internal medicine, medicine, Humans, Testosterone, Infertility, Male, Uremia, media_common, Sperm Count, medicine.diagnostic_test, business.industry, General Medicine, Luteinizing Hormone, Middle Aged, medicine.disease, Kidney Transplantation, Spermatozoa, Prolactin, Surgery, Semen Analysis, Transplantation, Sperm Motility, Kidney Failure, Chronic, Follicle Stimulating Hormone, business, Kidney disease
Abstract

To examine the semen quality of patients with uraemia and renal transplant recipients, 40 patients with uraemia and 40 renal transplant recipients were included. According to their interval of post-transplantation, renal transplant recipients were subdivided into group A (22)or =2 years and group B (18)2 years. A total of 40 healthy men with normal fertility were included as the controls. Semen samples from all subjects were collected and analysed. The fertility index (FI) value was calculated. The FI value of the normal fertility men was 13.02 (14.26), that of the renal transplant recipient groups A and B were 5.53 (8.30) and 9.27 (22.49) respectively, while the FI of the patients with uraemia was 0.23 (0.76). Compared with the uraemia group, the FI values of renal transplant recipient group either group A or group B were significantly better (P0.01). However, compared with the normal control group, the FI values of renal transplant recipient group A were lower (P0.01), while there was no significant difference between group B and the control group (P0.05). In conclusion, the FI of renal transplant recipients was recovered close to the level of healthy men with normal fertility 2 years after transplantation.

Published
2009
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44. First-principles investigations of Cr doping effects on electronic structure and magnetic properties in Sr2FeReO6

Author

L F Chen, X F Zhu, and Q.F. Li

Subjects
Physics, Moment (mathematics), Magnetic moment, Condensed matter physics, Formula unit, Doping, General Physics and Astronomy, Electronic structure, Half-metal, Inductive coupling, Spin magnetic moment
Abstract

The electronic structures and magnetic properties of double perovskites Sr2Fe1−xCrxReO6 ( x = 0.0 , 0.25, 0.5, 0.75, 1.0) have been studied within the local spin density approximation (LSDA) and LSDA + U schemes. The calculated results reveal that with increasing Cr content the cell volume shrinks 2.61%; the Fe/Cr site magnetic moment decreases while the Re-site moment increases. The total spin magnetic moment linearly decreases with the Cr doping from 3.00 μ B for x = 0.00 down to 1.00 μ B for x = 1.00 per formula unit. The magnetic coupling constants increase with increasing x. The electronic structure calculations indicate that the electronic concentration in the Re spin-down subband slightly increases resulting from the increase of bonding–antibonding interaction between the localised and the delocalised states in spin-down band; the coupling of O- 2 p ↑ and transition-metal- 3 d ↑ is substantially enhanced with the Cr doping. We discuss the origin of the anomalously high T C of Cr-doped Sr2FeReO6 compounds in terms of band hybridization effects.

Published
2008
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45. Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

Author

Klionsky, D.J. Abdelmohsen, K. Abe, A. Abedin, M.J. Abeliovich, H. Arozena, A.A. Adachi, H. Adams, C.M. Adams, P.D. Adeli, K. Adhihetty, P.J. Adler, S.G. Agam, G. Agarwal, R. Aghi, M.K. Agnello, M. Agostinis, P. Aguilar, P.V. Aguirre-Ghiso, J. Airoldi, E.M. Ait-Si-Ali, S. Akematsu, T. Akporiaye, E.T. Al-Rubeai, M. Albaiceta, G.M. Albanese, C. Albani, D. Albert, M.L. Aldudo, J. Algül, H. Alirezaei, M. Alloza, I. Almasan, A. Almonte-Beceril, M. Alnemri, E.S. Alonso, C. Altan-Bonnet, N. Altieri, D.C. Alvarez, S. Alvarez-Erviti, L. Alves, S. Amadoro, G. Amano, A. Amantini, C. Ambrosio, S. Amelio, I. Amer, A.O. Amessou, M. Amon, A. An, Z. Anania, F.A. Andersen, S.U. Andley, U.P. Andreadi, C.K. Andrieu-Abadie, N. Anel, A. Ann, D.K. Anoopkumar-Dukie, S. Antonioli, M. Aoki, H. Apostolova, N. Aquila, S. Aquilano, K. Araki, K. Arama, E. Aranda, A. Araya, J. Arcaro, A. Arias, E. Arimoto, H. Ariosa, A.R. Armstrong, J.L. Arnould, T. Arsov, I. Asanuma, K. Askanas, V. Asselin, E. Atarashi, R. Atherton, S.S. Atkin, J.D. Attardi, L.D. Auberger, P. Auburger, G. Aurelian, L. Autelli, R. Avagliano, L. Avantaggiati, M.L. Avrahami, L. Azad, N. Awale, S. Bachetti, T. Backer, J.M. Bae, D.-H. Bae, J.-S. Bae, O.-N. Bae, S.H. Baehrecke, E.H. Baek, S.-H. Baghdiguian, S. Bagniewska-Zadworna, A. Bai, H. Bai, J. Bai, X.-Y. Bailly, Y. Balaji, K.N. Balduini, W. Ballabio, A. Balzan, R. Banerjee, R. Bánhegyi, G. Bao, H. Barbeau, B. Barrachina, M.D. Barreiro, E. Bartel, B. Bartolomé, A. Bassham, D.C. Bassi, M.T. Bast, R.C., Jr. Basu, A. Batista, M.T. Batoko, H. Battino, M. Bauckman, K. Baumgarner, B.L. Bayer, K.U. Beale, R. Beaulieu, J.-F. Beck, G.R., Jr. Becker, C. Beckham, J.D. Bédard, P.-A. Bednarski, P.J. Begley, T.J. Behl, C. Behrends, C. Behrens, G.M.N. Behrns, K.E. Bejarano, E. Belaid, A. Belleudi, F. Bénard, G. Berchem, G. Bergamaschi, D. Bergami, M. Berkhout, B. Berliocchi, L. Bernard, A. Bernard, M. Bernassola, F. Bertolotti, A. Bess, A.S. Besteiro, S. Bettuzzi, S. Bhalla, S. Bhattacharyya, S. Bhutia, S.K. Biagosch, C. Bianchi, M.W. Biard-Piechaczyk, M. Billes, V. Bincoletto, C. Bingol, B. Bird, S.W. Bitoun, M. Bjedov, I. Blackstone, C. Blanc, L. Blanco, G.A. Blomhoff, H.K. Boada-Romero, E. Böckler, S. Boes, M. Boesze-Battaglia, K. Boise, L.H. Bolino, A. Boman, A. Bonaldo, P. Bordi, M. Bosch, J. Botana, L.M. Botti, J. Bou, G. Bouché, M. Bouchecareilh, M. Boucher, M.-J. Boulton, M.E. Bouret, S.G. Boya, P. Boyer-Guittaut, M. Bozhkov, P.V. Brady, N. Braga, V.M.M. Brancolini, C. Braus, G.H. Bravo-San-Pedro, J.M. Brennan, L.A. Bresnick, E.H. Brest, P. Bridges, D. Bringer, M.-A. Brini, M. Brito, G.C. Brodin, B. Brookes, P.S. Brown, E.J. Brown, K. Broxmeyer, H.E. Bruhat, A. Brum, P.C. Brumell, J.H. Brunetti-Pierri, N. Bryson-Richardson, R.J. Buch, S. Buchan, A.M. Budak, H. Bulavin, D.V. Bultman, S.J. Bultynck, G. Bumbasirevic, V. Burelle, Y. Burke, R.E. Burmeister, M. Bütikofer, P. Caberlotto, L. Cadwell, K. Cahova, M. Cai, D. Cai, J. Cai, Q. Calatayud, S. Camougrand, N. Campanella, M. Campbell, G.R. Campbell, M. Campello, S. Candau, R. Caniggia, I. Cantoni, L. Cao, L. Caplan, A.B. Caraglia, M. Cardinali, C. Cardoso, S.M. Carew, J.S. Carleton, L.A. Carlin, C.R. Carloni, S. Carlsson, S.R. Carmona-Gutierrez, D. Carneiro, L.A.M. Carnevali, O. Carra, S. Carrier, A. Carroll, B. Casas, C. Casas, J. Cassinelli, G. Castets, P. Castro-Obregon, S. Cavallini, G. Ceccherini, I. Cecconi, F. Cederbaum, A.I. Ceña, V. Cenci, S. Cerella, C. Cervia, D. Cetrullo, S. Chaachouay, H. Chae, H.-J. Chagin, A.S. Chai, C.-Y. Chakrabarti, G. Chamilos, G. Chan, E.Y.W. Chan, M.T.V. Chandra, D. Chandra, P. Chang, C.-P. Chang, R.C.-C. Chang, T.Y. Chatham, J.C. Chatterjee, S. Chauhan, S. Che, Y. Cheetham, M.E. Cheluvappa, R. Chen, C.-J. Chen, G. Chen, G.-C. Chen, G. Chen, H. Chen, J.W. Chen, J.-K. Chen, M. Chen, M. Chen, P. Chen, Q. Chen, Q. Chen, S.-D. Chen, S. Chen, S.S.-L. Chen, W. Chen, W.-J. Chen, W.Q. Chen, W. Chen, X. Chen, Y.-H. Chen, Y.-G. Chen, Y. Chen, Y. Chen, Y. Chen, Y.-J. Chen, Y.-Q. Chen, Y. Chen, Z. Chen, Z. Cheng, A. Cheng, C.H.K. Cheng, H. Cheong, H. Cherry, S. Chesney, J. Cheung, C.H.A. Chevet, E. Chi, H.C. Chi, S.-G. Chiacchiera, F. Chiang, H.-L. Chiarelli, R. Chiariello, M. Chieppa, M. Chin, L.-S. Chiong, M. Chiu, G.N.C. Cho, D.-H. Cho, S.-G. Cho, W.C. Cho, Y.-Y. Cho, Y.-S. Choi, A.M.K. Choi, E.-J. Choi, E.-K. Choi, J. Choi, M.E. Choi, S.-I. Chou, T.-F. Chouaib, S. Choubey, D. Choubey, V. Chow, K.-C. Chowdhury, K. Chu, C.T. Chuang, T.-H. Chun, T. Chung, H. Chung, T. Chung, Y.-L. Chwae, Y.-J. Cianfanelli, V. Ciarcia, R. Ciechomska, I.A. Ciriolo, M.R. Cirone, M. Claerhout, S. Clague, M.J. Cl� ria, J. Clarke, P.G.H. Clarke, R. Clementi, E. Cleyrat, C. Cnop, M. Coccia, E.M. Cocco, T. Codogno, P. Coers, J. Cohen, E.E.W. Colecchia, D. Coletto, L. Coll, N.S. Colucci-Guyon, E. Comincini, S. Condello, M. Cook, K.L. Coombs, G.H. Cooper, C.D. Cooper, J.M. Coppens, I. Corasaniti, M.T. Corazzari, M. Corbalan, R. Corcelle-Termeau, E. Cordero, M.D. Corral-Ramos, C. Corti, O. Cossarizza, A. Costelli, P. Costes, S. Cotman, S.L. Coto-Montes, A. Cottet, S. Couve, E. Covey, L.R. Cowart, L.A. Cox, J.S. Coxon, F.P. Coyne, C.B. Cragg, M.S. Craven, R.J. Crepaldi, T. Crespo, J.L. Criollo, A. Crippa, V. Cruz, M.T. Cuervo, A.M. Cuezva, J.M. Cui, T. Cutillas, P.R. Czaja, M.J. Czyzyk-Krzeska, M.F. Dagda, R.K. Dahmen, U. Dai, C. Dai, W. Dai, Y. Dalby, K.N. Valle, L.D. Dalmasso, G. D'amelio, M. Damme, M. Darfeuille-Michaud, A. Dargemont, C. Darley-Usmar, V.M. Dasarathy, S. Dasgupta, B. Dash, S. Dass, C.R. Davey, H.M. Davids, L.M. Dávila, D. Davis, R.J. Dawson, T.M. Dawson, V.L. Daza, P. de Belleroche, J. de Figueiredo, P. de Figueiredo, R.C.B.Q. de la Fuente, J. De Martino, L. De Matteis, A. De Meyer, G.R.Y. De Milito, A. De Santi, M. de Souza, W. De Tata, V. De Zio, D. Debnath, J. Dechant, R. Decuypere, J.-P. Deegan, S. Dehay, B. Del Bello, B. Del Re, D.P. Delage-Mourroux, R. Delbridge, L.M.D. Deldicque, L. Delorme-Axford, E. 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Published
2016

46. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Author

Klionsky, D.J. Abdelmohsen, K. Abe, A. Abedin, M.J. Abeliovich, H. Arozena, A.A. Adachi, H. Adams, C.M. Adams, P.D. Adeli, K. Adhihetty, P.J. Adler, S.G. Agam, G. Agarwal, R. Aghi, M.K. Agnello, M. Agostinis, P. Aguilar, P.V. Aguirre-Ghiso, J. Airoldi, E.M. Ait-Si-Ali, S. Akematsu, T. Akporiaye, E.T. Al-Rubeai, M. Albaiceta, G.M. Albanese, C. Albani, D. Albert, M.L. Aldudo, J. Algül, H. Alirezaei, M. Alloza, I. Almasan, A. Almonte-Beceril, M. Alnemri, E.S. Alonso, C. Altan-Bonnet, N. Altieri, D.C. Alvarez, S. Alvarez-Erviti, L. Alves, S. Amadoro, G. Amano, A. Amantini, C. Ambrosio, S. Amelio, I. Amer, A.O. Amessou, M. Amon, A. An, Z. Anania, F.A. Andersen, S.U. Andley, U.P. Andreadi, C.K. Andrieu-Abadie, N. Anel, A. Ann, D.K. Anoopkumar-Dukie, S. Antonioli, M. Aoki, H. Apostolova, N. Aquila, S. Aquilano, K. Araki, K. Arama, E. Aranda, A. Araya, J. Arcaro, A. Arias, E. Arimoto, H. Ariosa, A.R. Armstrong, J.L. Arnould, T. Arsov, I. Asanuma, K. Askanas, V. Asselin, E. Atarashi, R. Atherton, S.S. Atkin, J.D. Attardi, L.D. Auberger, P. Auburger, G. Aurelian, L. Autelli, R. Avagliano, L. Avantaggiati, M.L. Avrahami, L. Azad, N. Awale, S. Bachetti, T. Backer, J.M. Bae, D.-H. Bae, J.-S. Bae, O.-N. Bae, S.H. Baehrecke, E.H. Baek, S.-H. Baghdiguian, S. Bagniewska-Zadworna, A. Bai, H. Bai, J. Bai, X.-Y. Bailly, Y. Balaji, K.N. Balduini, W. Ballabio, A. Balzan, R. Banerjee, R. Bánhegyi, G. Bao, H. Barbeau, B. Barrachina, M.D. Barreiro, E. Bartel, B. Bartolomé, A. Bassham, D.C. Bassi, M.T. Bast, R.C., Jr. Basu, A. Batista, M.T. Batoko, H. Battino, M. Bauckman, K. Baumgarner, B.L. Bayer, K.U. Beale, R. Beaulieu, J.-F. Beck, G.R., Jr. Becker, C. Beckham, J.D. Bédard, P.-A. Bednarski, P.J. Begley, T.J. Behl, C. Behrends, C. Behrens, G.M.N. Behrns, K.E. Bejarano, E. Belaid, A. Belleudi, F. Bénard, G. Berchem, G. Bergamaschi, D. Bergami, M. Berkhout, B. Berliocchi, L. Bernard, A. Bernard, M. Bernassola, F. Bertolotti, A. Bess, A.S. Besteiro, S. Bettuzzi, S. Bhalla, S. 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Zhang, J. Zhang, J.-P. Zhang, L. Zhang, L. Zhang, L. Zhang, M.-Y. Zhang, X. Zhang, X.D. Zhang, Y. Zhang, Y. Zhang, Y. Zhang, Y. Zhang, Y. Zhao, M. Zhao, W.-L. Zhao, X. Zhao, Y.G. Zhao, Y. Zhao, Y. Zhao, Y.-X. Zhao, Z. Zhao, Z.J. Zheng, D. Zheng, X.-L. Zheng, X. Zhivotovsky, B. Zhong, Q. Zhou, G.-Z. Zhou, G. Zhou, H. Zhou, S.-F. Zhou, X.-J. Zhu, H. Zhu, H. Zhu, W.-G. Zhu, W. Zhu, X.-F. Zhu, Y. Zhuang, S.-M. Zhuang, X. Ziparo, E. Zois, C.E. Zoladek, T. Zong, W.-X. Zorzano, A. Zughaier, S.M.

Published
2016

47. Carbon-Assisted Growth of SiOx Nanowires

Author

X.-F. Zhu, Yiping Zhao, and Shihong Li

Subjects
Materials science, Nanostructure, Nanowire, Zno nanowires, chemistry.chemical_element, Nanotechnology, Surfaces, Coatings and Films, Si substrate, chemistry, Nanofiber, Materials Chemistry, Physical and Theoretical Chemistry, Vapor–liquid–solid method, Carbon
Abstract

During attempts to fabricate ZnO nanowires, we accidentally observed the growth of SiOx nanowires on Au-coated Si substrate. Detailed characterizations on the resulting nanowires were carried out b...

Published
2004
Full Text
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48. Relationship between leptin and chronic inflammatory state in uremic patients

Author

D-Z, Peng, X-W, Liu, L, Huang, X-F, Zhu, Y-Q, Zheng, and L-X, Wang

Subjects
Adult, Inflammation, Leptin, Male, Neutrophils, Middle Aged, Body Mass Index, Young Adult, Phagocytosis, Humans, Urea, Female, Inflammation Mediators, Aged, Uremia
Abstract

To explore the relation between high leptin and inflammation in uremic patients.A group of 73 uremic patients in dialysis center of our Department were assigned as uremic group; a group of 30 healthy persons who were examined over the same period were regarded as control group. The level of body mass index (BMI), serum creatinine (SCr), blood urea nitrogen (BUN), leptin, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10, and the neutrophils phagocytosis function were compared in two groups.BMI and IL-10 of uremic group were lower than the control group. The levels of SCr, BUN, leptin, TNF-α, IL-6 of uremic group were higher than the control group (p0.05). The neutrophils phagocytosis function in uremic group significantly decreases, compared to control group (p0.05). Using one-way ANOVA analysis, serum leptin was positively correlated with the level of TNF-α, IL-6 (r = 0.58, 1.00 respectively, p0.05), and was negatively correlated with the level of IL-10 (r = -0.45, p0.05).The high level of leptin and correlated inflammation were involved in the initiation and development of uremia; moreover, leptin was an important mediator.

Published
2014

49. IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF NINE CYST NEMATODE POPULATIONS FROM MAIN FIELD CROPS IN CHINA.

Author

Y. X. Feng, D. Wang, Y. H. Xuan, L. J. Chen, Y. Y. Wang, X. Y. Liu, Y. Chen, X. P. Lu, X. F. Zhu, and Y. X. Duan

Subjects
NEMATODES, PHYLOGENY, CROPS, CYSTS (Pathology), POLYMERASE chain reaction
Abstract

Cyst-forming nematodes, a major category of plant parasitic nematodes, cause severe yield losses in many crop plants and are a serious threat to food production in China. However, the reports focused on identification of species abundance and distribution of cyst nematodes on main crops in China are fewer. During 2012-2014, nine populations of cyst nematodes collected from various locations in major crop cultivation areas of China were identified as four species (Heterodera glycines, H. avenae, H. filipjevi, H. elachista) based on morphology and molecular analysis. H. filipjevi and H. elachista were first discovered in Anhui and Jiangxi Provinces, respectively. In addition, a PCR-ITS-RFLP method was applied to distinguish all the studied populations, which revealed molecular characteristics of cyst nematode species on major field crops in China. The present study broadens the knowledge regarding distributions and molecular features of vital Heterodera species and populations, and will also provide useful information for early protection of cystnematodes in China. [ABSTRACT FROM AUTHOR]

Published
2018

50. Lapatinib, a dual inhibitor of epidermal growth factor receptor and human epidermal growth factor receptor 2, potentiates the antitumor effects of cisplatin on esophageal carcinoma

Author

X F, Guo, X F, Zhu, G S, Zhong, and B G, Deng

Subjects
Esophageal Neoplasms, Receptor, ErbB-2, Carcinoma, Antineoplastic Agents, Apoptosis, Drug Synergism, Lapatinib, ErbB Receptors, G2 Phase Cell Cycle Checkpoints, Cell Line, Tumor, Quinazolines, Humans, M Phase Cell Cycle Checkpoints, Cisplatin, Cell Proliferation, Signal Transduction
Abstract

Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) overexpression occurs in over 30% of esophageal carcinomas. Combination therapies of EGFR- and HER2-targeting agents with cytotoxic agents are considered a potential therapeutic strategy for esophageal cancer. The antitumor effects of lapatinib, a dual tyrosine kinase inhibitor of EGFR and HER2, cisplatin alone, and the combination of the two drugs on esophageal cancer cells were evaluated. The growth inhibition activity of lapatinib, cisplatin, and lapatinib plus cisplatin was measured by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assays, and the combination index values were calculated. Additionally, cell cycle distribution and cell apoptosis treated with lapatinib or cisplatin alone and the combination of the two drugs were detected by flow cytometry analysis. The activation of EGFR and HER2 signaling pathways was monitored by Western blot analysis. These experimental data showed that the combination of lapatinib and cisplatin synergistically inhibited cell proliferation and exhibited an enhanced pro-apoptotic effect on esophageal cancer cells. The underlying mechanisms of potentiated effects of combined treatment were associated with reduced phosphorylation of EGFR and HER2, and the downstream signaling molecules AKT and extracellular regulated protein kinases (ERK). Our findings indicated that the combination of lapatinib and cisplatin is one of the promising treatment strategies for esophageal carcinomas with EGFR and HER2 overexpression.

Published
2012

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