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Aims: High‐expressed miR‐330‐3p in gestational diabetes mellitus (GDM) patients was reported. However, the role and mechanism of miR‐330‐3p in GDM are rarely reported. In this research, we aim to investigate the effects of miR‐330‐3p on GDM. Methods: MiR‐330‐3p expression in the GDM patients' blood was determined by q‐PCR. Blood glucose of blood samples was detected using blood glucose detection kits. Glucokinase (GCK) was confirmed to be a target gene of miR‐330‐3p by bioinformatics and luciferase analysis. Correlations between miR‐330‐3p with GCK and blood glucose were analyzed by Pearson correlation analysis. After INS‐1 cells were treated with glucose and transfected with mimic, inhibitor or siGCK, GCK expression was detected by western blot, and q‐PCR, enzyme‐linked immunosorbent assays, cell counting kit‐8 and Annexin‐V/propidium iodide were conducted to examine the expression of insulin, cell viability and apoptosis. Results: MiR‐330‐3p was high‐expressed in GDM patients' blood, while GCK was low‐expressed. The miR‐330‐3p expression level positively correlated with blood glucoseand and it was highly expressed in glucose‐treated INS‐1 cells (11 and 22 mmol/L), while miR‐330‐3p expression negatively correlated with GCK expression. GCK expression was inhibited by miR‐330‐3p mimic and enhanced by the miR‐330‐3p inhibitor. MiR‐330‐3p mimic inhibited INS‐1 cells' insulin expression, cell viability and induced apoptosis. Yet miR‐330‐3p inhibitor and siGCK exhibited opposite effects which miR‐330‐3p mimic and GCK played on INS‐1 cells. In addition, siGCK reversed the effect of miR‐330‐3p inhibitor on INS‐1 cells. Conclusion: Our findings proved that miR‐330‐3p targeting GCK lead to the dysfunction of INS‐1 cells in GDM, and could become a therapeutic target for GDM treatment. [ABSTRACT FROM AUTHOR]

Microwave photonic synthetic aperture radar (MWP-SAR) offers a larger signal bandwidth than conventional SAR, and its theoretical resolution can be improved to centimeter level. To achieve same order of magnitude resolution in the azimuth direction, long synthetic aperture is always required, which results in extremely high requirements for the accuracy of imaging procedure. Compared with the conventional SAR imaging algorithms, two major problems should be considered: 1) the scattering characteristics of target might vary from the frequency of signal and the angle of incidence, which seriously affects the coherence of received echo and 2) the imaging of the MWP-SAR system is more sensitive to motion errors and therefore requires higher accuracy of motion compensation processing. To solve the above issues, a high-precision imaging method for MWP-SAR is proposed. First, based on the attribute scattering center (ASC) model, this article analyzes the target scattering characteristics with different frequencies and incident angles. Then, according to the influence of scattering phase on MWP-SAR imaging, an optimal subaperture division algorithm is proposed to guarantee the coherence of each subaperture data and better imaging results. Furthermore, to compensate for the effects of high-order motion errors, this article proposes a motion error estimation algorithm based on subimage registration, where the full-aperture high-order motion errors can be divided into multiple linear components, and the flight trajectory of platform can be accurately reconstructed. Finally, a full-aperture time-domain high-precision imaging method is presented, and the effectiveness of the proposed formation is verified by both simulation and actual airborne MWP-SAR data processing. [ABSTRACT FROM AUTHOR]

Background: Recently Cyclophilin A (CypA) was identified as a candidate target protein in gastric carcinoma. However, the role of CypA in gastric cancer (GC) has not been investigated extensively so far. Aim: The purpose of this study was to determine the expression pattern of CypA in human GC, and to explore the effects of suppressed CypA expression on cell proliferation and xenografted tumor growth of gastric cancer. Methods: In the present study, we detected the expression pattern of CypA in human GC by immunohistochemistry analysis. Further, the RNAi method was used to silence CypA, and colony formation assay, growth curves, cell cycle and mouse xenograft were analysed. Results: An elevated expression of CypA in GC tissues compared with normal gastric mucosa was observed, especially in TNM stage-I and intestinal type of tumor. CypA was overexpressed in most GC cell lines and endogenous expression of CypA correlated with cell growth phenotypes. Transient suppression of CypA reduced the proliferation of BGC-823 and SGC-7901 GC cell lines. Exogenous CypA promoted the proliferation of NCI-N87 GC cells in a concentration dependent manner. Further study revealed that stable CypA silencing inhibited the proliferation, prevented cell cycle and reduced autophagy of BGC-823 GC cells in vitro through suppressing the ERK1/2 signal pathway. Stable CypA silencing also inhibited the growth of xenografted tumor of BGC-823 GC cell in nude mice. Conclusions: These results indicate a special function mode for CypA of playing more important roles in the early stage of gastric tumorigenesis and suggest CypA as a new molecular target of diagnosis and treatment for GC patients. [ABSTRACT FROM AUTHOR]

Bistatic synthetic aperture radar (BiSAR) has received more and more attention because of its forward-looking imaging capability and configuration flexibility. For BiSAR moving target imaging (MTIm), its noncooperative motion leads to unknown range cell migration (RCM) and additional phase modulation. Consequently, MTIm in BiSAR faces two main challenges: 1) the unknown RCM correction and Doppler parameter estimation are tightly coupled and 2) the Doppler parameters of the extended moving target’s different scattering points are different, i.e., the Doppler parameters are spatially variant. To cope with these problems, an optimal polar format refocusing method for bistatic SAR MTIm is proposed. First, the main part of tight coupling and spatial variation effects caused by the BiSAR platforms is eliminated, while the moving target is 2-D defocused and shifted. Then, we analyze the characteristics of 2-D defocused and shifted of the moving target in BiSAR and give the analytical expressions. Basis this, a new bistatic polar format transformation is introduced, in which the degree of freedom of defocusing result is reduced from 2-D to only 1-D. After that, the parameter estimation and refocusing issues are transformed into a constrained optimization problem (COP), and differential evolution (DE) is applied to solve the COP and obtain the refocusing results. Finally, considering the spatial variation of the extended moving target, compensation processing is performed to relocate each scattering point. Numerical simulations verify the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

AbstractLow-dose metronomic (LDM) chemotherapy represents a new strategy to treat solid tumors by stronger antiangiogenic activity and less side-effects, especially in combination with other antiangiogenic agents. The aim of the study is to investigate the antiangiogenic effect of docetaxel alone and combined with (−)-epigallocatechin-3-gallate (EGCG) in preclinical settings of gastric cancer. BGC-823 human gastric cancer xenograft model was used, and tumor growth, side-effects of mice were closely monitored. Expression of vascular endothelial growth factor and CD31 were observed by immunohistochemistry, and microvessel density of the tumor tissues was assessed by CD31 immunohistochemical analysis. Our results indicated that LDM docetaxel inhibited angiogenesis and growth of gastric cancer with less toxicity, and the effects were further enhanced by the concurrent administration of EGCG. Our study, for the first time, rationally demonstrated that LDM docetaxel treatment used alone or combined with EGCG is effective and safe in preclinical settings of gastric cancer. Our data suggest that LDM docetaxel used alone or combined with EGCG may be an innovative and promising therapeutic strategy in the experimental treatment of human gastric cancer. [ABSTRACT FROM AUTHOR]

Objective. To explore the effect of acupoint application of Chinese medicine on children's height and bone age. Methods. Altogether, 120 children with a short stature treated in our hospital from September 2017 to September 2018 were divided into the control group (CG, n = 60) and the observation group (OG, n = 60) according to the random number table method. The children in CG were given healthy diet and exercise plans and supplemented with daily vitamin intake. The OG was treated with acupoint application of Chinese medicine on the basis of the CG. The clinical efficacy of the CG and the OG of children after treatment was observed. The height increment, growth rate, and bone age of children were compared before and after treatment. The levels of IGF-1 and 25-(OH)D in the serum of children before and after treatment were tested. According to the clinical curative effect after treatment, the children were divided into good curative effect group (markedly effective + effective) and poor curative effect group (ineffective). Logistics regression analysis was applied to analyze the risk factors. Results. Compared with the CG, the curative effect on the OG was evidently improved (P < 0.05). In addition, the height increment, the growth speed, and the bone age of the OG increased evidently (P < 0.05). Compared with the CG, the expression of IGF-1 and 25-(OH)D of the OG elevated (P < 0.05). Serum IGF-1 and 25-(OH)D concentrations were positively correlated with growth rate and bone age (P < 0.05). Risk factors analysis showed that disease course, IGF-1, 25-(OH)D expression, and heredity were the risk factors affecting the curative effect on children. Conclusion. Acupoint application of Chinese medicine has effect on the height and bone age of children with short stature, which is worthy of clinical promotion. In addition, early treatment should be carried out to improve the clinical efficacy of children. [ABSTRACT FROM AUTHOR]

A hydroponic experiment was carried out utilizing four barley genotypes with differing salt tolerances to investigate the influence of salinity (NaCl) and cadmium (Cd) on the activities of antioxidant enzymes, sodium (Na) and Cd concentrations, and accumulation in plants. The activities of the two antioxidant enzymes, superoxide dismutase (SOD) and peroxidase (POD), were significantly increased when plants were exposed to both Na and Cd stresses for all genotypes, relative to the control. The increased enzymatic activity was more predominant with a prolonged time of stress exposure. The combined stress (NaCl+Cd) led to a further increase in POD activity, but had little effect on SOD activity. Two salt-tolerant genotypes, ‘Gebeina’ and ‘Zhou 1,’ showed a more rapid increase of POD and SOD activities than the two salt-sensitive genotypes, ‘Newgoutei’ and ‘Quzhou’ in response to the combined stress treatment. Additions of NaCl to the Cd-containing medium caused a significant reduction in both Cd concentration and accumulation. The extent of the reduction in Cd concentration was also dependent on genotypes. The salt-tolerant genotypes had lower Na concentrations than sensitive genotypes, and the effect of Cd stress on Na concentration and accumulation varied with genotypes. It may be concluded that a significant interaction exists between Na and Cd in their influence on antioxidant enzyme activity and the accumulation of each element in the plant. [ABSTRACT FROM AUTHOR]