1. High Concentration of Insulin Promotes Apoptosis of Primary Cultured Rat Ovarian Granulosa Cells Via Its Increase in Extracellular HMGB1
- Author
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Xiao-Rong Ni, Rong-Hui Wang, Zhou-Jun Sun, and Guo-Hua Hu
- Subjects
endocrine system ,medicine.medical_specialty ,Time Factors ,endocrine system diseases ,medicine.medical_treatment ,Apoptosis ,Transfection ,HMGB1 ,Rats, Sprague-Dawley ,Pathogenesis ,Insulin resistance ,Internal medicine ,medicine ,Hyperinsulinemia ,Animals ,Humans ,Insulin ,Endocrine system ,HMGB1 Protein ,Cells, Cultured ,Granulosa Cells ,Dose-Response Relationship, Drug ,biology ,Polycystic ovary syndrome (PCOS) ,nutritional and metabolic diseases ,Obstetrics and Gynecology ,medicine.disease ,Polycystic ovary ,female genital diseases and pregnancy complications ,Up-Regulation ,Endocrinology ,Case-Control Studies ,biology.protein ,Female ,RNA Interference ,Insulin Resistance ,hormones, hormone substitutes, and hormone antagonists ,Polycystic Ovary Syndrome ,Signal Transduction - Abstract
Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age. Insulin resistance/hyperinsulinemia is a prevalent finding in women with PCOS, which indicates that insulin resistance/hyperinsulinemia may be an important player in the pathogenesis of the PCOS. However, the underlying mechanism of insulin resistance/hyperinsulinemia on the pathogenesis of the PCOS remains elusive. In this study, we found an increased high-mobility group box 1 (HMGB1) in the serum from women with PCOS having insulin resistance/hyperinsulinemia. Furthermore, we discovered that high concentration of insulin, which mimics insulin resistance model, promoted apoptosis in primary cultured rat ovarian granulosa cells (GCs) via its effect on the increase in extracellular HMGB1. Our data presented the first evidence that increased HMGB1 induced by insulin resistance/hyperinsulinemia promoted apoptosis of ovarian GCs, which provided new molecular basis for the PCOS pathogenesis.
- Published
- 2015