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In large-diameter shield tunnels, applying the double-layer lining structure can improve the load-bearing properties and maintain the stability of segmental lining. The secondary lining thickness is a key parameter in the design of a double lining structure, which is worth being explored. Based on an actual large-diameter shield tunnel, loading model tests are carried out to investigate the effect of the secondary lining thickness on the mechanical behaviours of the double lining structure. The test results show that within the range of secondary lining thicknesses discussed, the load-bearing limit of the double-layer lining increases with growing secondary lining thickness. As a passive support, the secondary lining acts as an auxiliary load-bearing structure by contacting the segment. And changes in secondary lining thickness have a significant effect on the contact state between the segment and secondary lining, with both the contact pressure level and the contact area between the two varying. For double-layer lining structures in large-diameter shield tunnels, it is proposed that the stiffness of the secondary lining needs to be matched to the stiffness of the segment, as this allows them to have a coordinated deformation and a good joint load-bearing effect.

Terrestrial dissolved organic matter (tDOM) holds great promise for controlling cyanobacteria blooms through watershed management. To identify tDOM that could inhibit the growth, photosynthesis and colony formation, unicellular Microcystis aeruginosa Kützing (FACHB-469) was cultivated and treated with varying concentrations of gallic acid, proline and tea polyphenols at different levels of iron. The results indicated that gallic acid and tea polyphenols could inhibit Microcystis growth by suppressing photosynthesis and colony formation by reducing extracellular polysaccharides (EPS) secretion. However, proline had no significant effect on the growth, photosynthesis, colony size and EPS content of Microcystis. Transcriptome analysis showed Microcystis may optimize the internal energy transfer mode of photosynthesis through the change of phycobilisome at different levels of iron. In addition, Microcystis adapted to different iron concentration environments by regulating the expression of genes associated with iron uptake and transport. These findings suggest that the effects of plant species on algal blooms should be considered in reforestation of watershed. This consideration necessitates finding a balance between the costs and benefits of controlling cyanobacteria blooms using tDOM.

Physical soil crust (PSC), a key component of surface soil structure, exists extensively in loess areas. PSC is considered to have a significant effect on soil detachment processes. However, the long-term effects and the corresponding mechanisms of PSC on soil detachment by overland flow are still not well understood, especially in natural environments. To investigate temporal variation in soil erosion resistance and the underlying factors during PSC formation, an 8 × 8-m soil plot was exposed to natural conditions in the Loess Plateau over a 524-day period spanning two rainy seasons and a winter between them. A flume test was conducted to determine soil detachment capacity (Dc) under six designed flow shear stress levels (5.66–22.11 Pa) using crusted (SC) and non-crusted (NSC) soil samples at different PSC development stages. Subsequently, two soil erosion resistance parameters, rill erodibility (Kr) and critical shear stress (τc), were calculated. Over time, in the SC and NSC treatments, Kr decreased from 0.516 to 0.120 s m−1 and 0.521 to 0.223 s m−1, respectively, while τc increased from 0.49 to 4.42 Pa and 0.26–2.46 Pa, respectively. Variation in soil erosion resistance was rapid in the first one to two months, and then slowed down, with slight fluctuations afterwards. In the SC treatment, Kr was 42% lower and τc was 67% greater than those in the NSC treatment. Soil properties changed greatly for both treatments. SCT increased from 0 to 7.09 mm in the SC treatment. Coh increased from 2.91 to 9.04 kPa and 3.01–4.78 kPa in SC and NSC treatments, respectively. Both soil erosion resistance parameters could be well predicted by SCT and Coh in the SC treatment (R2 ≥ 0.82), while their best predictor was Coh in the NSC treatment (R2 ≥ 0.90). The results demonstrate that PSC formation enhances soil erosion resistance in the soil detachment process in the loess region under natural conditions. Our study revealed the important role and complexity of PSC in the process of soil erosion, and provided theoretical and data support for accurate understanding and prediction of soil erosion.

Insufficient milk supply is a widespread issue faced by women globally and associated with a higher risk of health problems in infants and mothers. Hemerocallis citrina Baron, commonly known as daylily, is a perennial edible plant often used in traditional Asian cuisine to promote lactation. However, the active compound(s) and mechanism of its lactation-promoting effect remain unclear. This study aimed to confirm the traditional use of daylily in promoting lactation and investigate its potential active components and underlying molecular mechanisms. Our results showed that the aqueous extracts of H. citrina Baroni (HAE) significantly enhanced milk production, and the serum levels of lactation-related hormones, and promoted mammary gland development in lactating rats, as well as increased the levels of milk components in bovine mammary epithelial cells (BMECs) (p < 0.05). UHPLC-Q-Exactive Orbitrap-MS analysis revealed that hexamethylquercetin (HQ) is the representative flavonoid component in HAE, accounting for 42.66% of the total flavonoids. An integrated network pharmacology and molecular docking analysis suggested that HQ may be the potential active flavonoid in HAE that promotes lactation, possibly supporting lactation by binding to key target proteins such as STAT5A, PIK3CA, IGF1R, TP53, CCND1, BCL2, INS, AR, and DLD. Cell experiments further demonstrated that HQ could promote cell proliferation and the synthesis of milk proteins, lactose, and milk fat in BMECs. Transcriptomic analysis combined with a quantitative reverse transcription polymerase chain reaction (RT-qPCR) revealed that both HAE and HQ exert a lactation-promoting function mainly through regulating the expression of key genes in the PI3K-Akt signaling pathway.

Peroxymonosulfate (PMS, SO52−)-based oxidation is an efficient pathway for degrading organic pollutants, but it still suffers from slow degradation efficiency and low PMS utilization. In this work, we report the preparation of porous Fe-doped g-C3N4 catalysts by one-step thermal polymerization using urea and transition metal salts as precursors and investigate the effect of atmosphere conditions (air and nitrogen) on the catalytic performance. Systematic characterizations show that Fe-doped g-C3N4 prepared in air (FeNx-CNO) has a larger specific surface area (136.2 m2 g−1) and more oxygen vacancies than that prepared in N2 (FeNx-CNN, 74.2 m2 g−1), giving it more active sites to participate in the reaction. Meanwhile, FeNx-CNO inhibits the recombination of photogenerated carriers and improves the light utilization. The redox cycling of Fe(III) and Fe(II) species in the photocatalytic system ensures the continuous generation of SO5•− and SO4•−. Therefore, FeNx-CNO can remove CBZ up to 96% within 20 min, which is 3.4 times higher than that of CNO and 3.1 times higher than that of FeNx-CNN, and the degradation efficiency can still retain 93% after 10 cycles of reaction. This study provides an economical and efficient method for photocatalysis in the degradation of medicines in contaminated water.

Macrophytes are crucial in maintaining the equilibrium of aquatic ecosystems. However, the pattern of macrophyte-derived caffeic acid (CA) release under heavy metal stress is yet to be fully understood. More importantly, due to its functional groups, CA may be a precursor to the formation of disinfection by-products, posing threats to water ecology and even safety of human drinking water. This study analyzed the responses of CA released by Vallisneria natans (V. natans) and Pistia stratiotes (P. Stratiotes) when exposed to Cu2+ and Mn2+ stress. Additionally, the CA levels in two constructed wetland ponds were detected and the degradation kinetics of CA during chlorination were investigated. Results indicated that CA occurred in two constructed wetland ponds with the concentrations of 44.727 μg/L (planted with V. natans) and 61.607 μg/L (planted with P. Stratiotes). Notably, heavy metal stress could significantly affect CA release from V. natans and P. Stratiotes. In general, under Cu2+ stress, V. natans secreted far more CA than under Mn2+ stress, the level could reach up to 435.303 μg/L. However, compared to V. natans, P. Stratiotes was less affected by Cu2+ and Mn2+ stress, releasing a maximum CA content of 55.582 μg/L under 5 mg/L Mn2+ stress. Aquatic macrophytes secreted more CA in response to heavy metal stresses and protected macrophytes from harmful heavy metals. CA degradation followed the pseudo first-order kinetics model, and the chlorination of CA conformed to a second-order reaction. The reaction rate significantly accelerated as NaClO, pH, temperature and Br- concentration increased. A new pathway for CA degradation and a new DBP 2, 2, 3, 3-tetrachloropropanal were observed. These findings pointed at a new direction into the adverse effect of CA, potentially paving the way for new strategies to solve drinking water safety problems.

We investigated the effects of ultrasound-assisted ethylenediaminetetraacetic acid (EDTA) and citric acid (CA) washing on heavy metal (HM) removal, residual HM mobility, and sewage sludge quality. EDTA and CA washing of sewage sludge successfully reduced the total concentration of HMs after one round of washing, but the mobility of residual HMs increased significantly. The eluate had a high concentration of HMs and nutrients (nitrogen, phosphorus, potassium, and total organic carbon), although the nutritional content of the sludge remained high. The three-phase ratio of the sludge after six rounds of washing by CA was closest to the ideal three-phase ratio, and the degree of influence on the physical structure of the soil after a land application was reduced, according to the fluctuation of generalized soil structure index (GSSI) and soil three-phase structure distance (STPSD) values. The results indicate that CA as an environmental-friendly washing agent can be the superior choice for sludge HM washing; single washing of sewage sludge may increase the mobility of residual HMs, so multiple washings should be considered for land application of sludge. HIGHLIGHTS Ultrasound-assisted chemical washing is a useful and efficient solution to remove HMs from sewage sludge.; The higher concentration (≥0.4 M) of biodegradable CA eluent could effectively reduce HMs in the sewage sludge compared to the traditional non-biodegradable EDTA eluent.;

There are numerous applications of terahertz (THz) imaging in many fields. However, current THz imaging is generally based on scanning technique due to the limited intensity of the THz sources. Thus, it takes a long time to obtain a frame image of the target and cannot meet the requirement of fast THz imaging. Here, we demonstrate a single-shot direct THz imaging strategy based on a broadband intense THz source with a frequency range of 0.1~23 THz and a THz camera with a frequency response range of 1~7 THz. This THz source was generated from the laser–plasma interaction, with its central frequency at ~12 THz. The frame rate of this imaging system was 8.5 frames per second. The imaging resolution reached 146.2 μm. With this imaging system, a single-shot THz image for a target object with a size of more than 7 cm was routinely obtained, showing a potential application for fast THz imaging. Furthermore, we proposed and tested an image enhancement algorithm based on an improved dark channel prior (DCP) theory and multi-scale retinex (MSR) theory to optimize the image brightness, contrast, entropy and peak signal-to-noise ratio (PSNR).

ABSTRACT: Yak milk is rich in essential milk proteins of nutritional and therapeutic value. In this study, whey proteins of milk from 3 yak breeds (Gannan, GN; Huanhu, HH; Maiwa, MW) in China were comprehensively identified and compared using a data-independent acquisition quantitative proteomics approach. A total of 632 proteins were identified in yak milk whey samples, in which immune-related proteins were abundant. Compared with other milks, more proteins were involved in oxidation-reduction process and with ATP binding. In addition, we identified 96, 155, and 164 differentially expressed proteins (DEP) for GN versus HH, GN versus MW, and HH versus MW, respectively. “Phagosome” and “complement and coagulation cascades” were the most significant pathways for DEP of GN versus HH and GN or HH versus MW yak milk based on the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Protein-protein interaction network analysis showed that DEP for the 3 comparisons had significant biological interactions but were associated with different functions. The results provide useful information on yak milk from different breeds in China, and elucidate the biological functions of yak milk proteins.

The exploitation of highly efficient solvent-free catalytic systems for the selective aerobic oxidation of benzylic compounds to produce corresponding ketones with molecular oxygen under mild conditions remains a great challenge in the chemical industry. In this work, Au-Pd nanoparticles supported on porous carbon catalysts were fabricated by the borax-mediated hydrothermal carbonization method and the chemical reduction method. The physicochemical properties of Au-Pd bimetallic samples were examined by XRD, N2 sorption, SEM, TEM, and XPS techniques. The Au-Pd nanoparticles have successfully immobilized on the spherical carbon support with a porous structure and large surface area. A solvent-free catalytic oxidation system was constructed to selectively convert indane into indanone with Au-Pd nanocatalysts and O2. In contrast with a monometallic Au or Pd catalyst, the resulting bimetallic Au-Pd catalyst could effectively activate O2 and exhibit improved catalytic activity in the controlled oxidation of indane into indanone under 1 bar O2. A total of 78% conversion and >99% selectivity toward indanone can be achieved under optimized conditions. The synergistic effect of Au and Pd and porous carbon support contributed to the high catalytic activity for aerobic benzylic compound oxidation. This work offers a promising application prospect of efficient and recyclable Au-Pd nanocatalysts in functional benzylic ketone production.

IntroductionIn this paper, microbiota analysis was determined to analyze the structure and difference of intestinal microbiota between LBMJ (late-onset breast milk jaundice) infants and healthy individuals.MethodsWe collected fresh fecal samples from 13 infants with LBMJ and 13 healthy individuals, then determined the intestinal microbiota by 16 s rRNA sequencing. The differences of microbiota structure, diversity and functional characteristics between the two groups were analyzed, and the correlation between dominant genus and TcB (transcutaneous bilirubin) value was calculated.ResultsIn this study, there were no significant differences in maternal demographic characteristics, neonatal status and macronutrients in breast milk between the two groups (p > 0.05). There are differences in the structure of intestinal microbiota between LBMJ and the control group. At the genus level, the relative abundance of Klebsiella in the case group is high (p

With the continuous improvement of people's living standards and health awareness, more and more attention has been paid to food safety. It follows that the research and management of the types and labels of food allergens also need to be more and more strict. Therefore, the regulation of food allergens needs to be more and more stringent. This summary reviews the current status of food allergen testing methods and standards in China. Also, the labelling of food allergen types in China, the Codex Alimentarius Commission, the United States, the European Union, Canada and Japan is systematically summarized. By systematically reviewing the labeling management of food allergens in various countries and regions, it is hoped to provide reference for Chinese food allergen labeling management, and to continuously improve our country's food allergen detection methods and labeling standards.

Background: Vegetation restoration influences soil properties considerably, which in turn interact with microbial community structure and regulate ecosystem stability. However, the influence of soil physical properties on soil microbial community structure has been poorly characterized. Methods: We investigated changes in soil physicochemical properties and bacterial and fungal diversity and composition after natural restoration (grasslands (GL)) and afforestation (artificial forests (AF)) for more than 40 years in a karst faulted basin in China. Results: Compared with that in undamaged natural forests (NF), AF enhanced soil organic carbon and nutrient contents markedly, whereas GL improved soil water stable aggregate, bulk density, and pH. Alpha diversity analyses suggested that vegetation restoration had no significant effect on Chao 1 index and Shannon index of bacterial and fungal communities. However, according to the nonmetric multidimensional scaling analysis results, the dissimilarities in bacterial and fungal community composition between GL and NF were more distinct than those between AF and NF. Such diverse responses of bacterial community structure to vegetation restoration were largely explained by soil pH. Additionally, soil pH, porosity, bulk density, and field capacity were significantly correlated with fungal community structure (P < 0.05). Conclusion: Vegetation restoration in ecologically fragile areas should focus on understanding interactions between microbial community structure and soil chemical properties as well as physical properties.

The replacement of traditional fossil fuels by renewable energy sources (RESs) leads to the loss of power grid’s frequency support capability while reducing the greenhouse effect. To improve the primary frequency reserve (PFR) and the inertia response (IR) of the grid, a configuration method for an energy storage system (ESS) is proposed. The relationship between the maximum power fluctuation that the system can withstand and the proportion of RESs is deduced in this paper considering the frequency requirements. The capacity and the control parameters of the ESS under the third-order virtual synchronous generator (VSG) control strategy are configured to improve the system’s ability to promote energy consumption. Taking the unit power regulation and the inertia constant as references, the contribution of the ESS under different power fluctuations and different control parameters is analyzed. Finally, the simulation results show that the ESS with the proposed methodology has eminent control characteristics and flexible power flow, which can effectively improve the grid’s operating environment.

Hypertension is a major risk factor leading to cardiovascular disease, and is frequently treated with angiotensin I-converting enzyme (ACE) inhibitory peptides. The objective of this study was to separate and identify an ACE-inhibitory peptide from goat milk casein hydrolysates, and to evaluate its potential for improving angiotensin II (Ang II)-mediated adverse effects on vascular smooth muscle cells (VSMCs). A novel ACE-inhibitory peptide with the highest activity from the goat milk casein hydrolysates as determined by four steps of RP-HPLC was purified and identified as Phe-Pro-Gln-Tyr-Leu-Gln-Tyr-Pro-Tyr (FPQYLQYPY). The results of inhibitory kinetics studies indicated that the peptide was a non-competitive inhibitor against ACE. Gastrointestinal digest in vitro analysis showed that the hydrolysate of FPQYLQYPY was still active after digestion with gastrointestinal proteases. Moreover, we found that the peptide could significantly inhibit the proliferation and migration of Ang II-stimulated VSMCs. Further transcriptomic analysis revealed that differentially expressed genes (DEGs) were enriched in the cardiovascular disease-related pathways, and that the peptide may have the ability to regulate vascular remodeling. Our findings indicate the potential anti-hypertensive effects of FPQYLQYPY, as well-implicate its role in regulating vascular dysfunction.

Abstract Background The dedifferentiation of chondrocytes and the unstable chondrogenic differentiation status of pluripotent mesenchymal stem cells (MSCs) are immense issues in cell-based articular cartilage repair and regenerative strategies. Here, to improve the cartilage characteristics of seed cells, a double biomimetic acellular cartilage extracellular matrix (ACECM)-oriented scaffold was used to mimic the cartilage microenvironment for human umbilical cord Wharton’s jelly-derived MSCs (hWJMSCs) and primary cartilage cells (pACs) to regenerate hyaline cartilage. Methods A double biomimetic ACECM-oriented scaffold was created from the cartilage extracellular matrix of pig articular cartilage using pulverization decellularization freeze-drying procedures. hWJMSCs and pACs were co-cultured at ratios of 50:50 (co-culture group, ACCC), 0:100 (ACAC group) and 100:0 (ACWJ group) in the ACECM-oriented scaffold, and the co-culture system was implanted in a caprine model for 6 months or 9 months to repair full-thickness articular cartilage defects. The control groups, which had no cells, comprised the blank control (BC) group and the ACECM-oriented scaffold (AC) group. Gross morphology and magnetic resonance imaging (MRI) as well as histological and biomechanical evaluations were used to characterize the cartilage of the repair area. Results Relative to the control groups, both the gross morphology and histological staining results demonstrated that the neotissue of the ACCC group was more similar to native cartilage and better integrated with the surrounding tissue. Measurements of glycosaminoglycan content and Young’s modulus showed that the repair areas had more abundant cartilage-specific content and significantly higher mechanical strength in the ACCC group than in the control groups, especially at 9 months. On MRI, the T2-weighted signal of the repair area was homogeneous, and the oedema signal disappeared almost completely in the ACCC group at 9 months. HLA-ABC immunofluorescence staining demonstrated that hWJMSCs participated in the repair and regeneration of articular cartilage and escaped surveillance and clearance by the caprine immune system. Conclusion The structure and components of double biomimetic ACECM-oriented scaffolds provided a cartilage-like microenvironment for co-cultured seed cells and enhanced the biomechanics and compositions of neotissue. This co-culture system has the potential to overcome the dedifferentiation of passage chondrocytes and the unstable chondrogenic differentiation status of MSCs.

Traffic congestion detection plays an important role for road management. However, it is difficult to automatically report traffic congestion when it occurs in large-scale road network. One of key challenges for rapidly and precisely identifying early congestion is huge variations in appearance caused by illumination, weather, camera settings and other traffic conditions. To address it, we proposed a traffic-oriented model to classify congestion from large dataset of ultra-low frame rate video captured from traffic surveillance system. The proposed deeply supervised traffic congestion detector has two modules: attention proposal module and deeply supervised inception network. Specifically, within the shallow layers, the binary edge/corner density features are used in attention proposal module to generate the rang of interest (ROI) mask automatically. This strategy keeps the training process focusing on the congestion features without disturbances. Following the attention proposal module, a very deep structure based on the inception network was used together to effectively extract rich and discriminative features then detect traffic congestion. The approach was tested on a self-established dataset based on empirical data, which contains images captured from 14470 surveillance cameras for monitoring 5,215 km of freeway in Shaanxi province, China. The experimental results show that the accuracy of the proposed method could reach 95.77% considering various disturbances, conditions and other limitations, which is improved than unsupervised networks.

In order to deeply explore the transient stability mechanism of the AC/DC hybrid system, this paper analyzes the rotor angle stability of the two-area AC/DC hybrid system. The system is analyzed after subjecting it to large disturbances in the AC system and for different operating conditions, qualitatively and quantitatively. The influence of factors, such as the AC operating point of the system and the proportion of DC transmission power, have been considered for improving the rotor motion equation. Subsequently, the transient characteristics of the hybrid system are analyzed after being subjected to disturbances. The power angle stability margin index is obtained, based on which the transient characteristics after DC blocking are analyzed, and the coordinated control strategy with the least control cost is proposed. The results are verified using the two-area AC/DC parallel transmission system network model. The study provides the reference for ensuring the security and stability of the hybrid AC/DC power grid.

Monte Carlo simulations can quantify various types of DNA damage to evaluate the biological effects of ionizing radiation at the nanometer scale. This work presents a study simulating the DNA target response after proton irradiation. A chromatin fiber model and new physics constructors with the ELastic Scattering of Electrons and Positrons by neutral Atoms (ELSEPA) model were used to describe the DNA geometry and the physical stage of water radiolysis with the Geant4-DNA toolkit, respectively. Three key parameters (the energy threshold model for strand breaks, the physics model and the maximum distance to distinguish DSB clusters) of scoring DNA damage were studied to investigate the impact on the uncertainties of DNA damage. On the basis of comparison of our results with experimental data and published findings, we were able to accurately predict the yield of various types of DNA damage. Our results indicated that the difference in physics constructor can cause up to 56.4% in the DNA double-strand break (DSB) yields. The DSB yields were quite sensitive to the energy threshold for strand breaks (SB) and the maximum distance to classify the DSB clusters, which were even more than 100 times and four times than the default configurations, respectively.

This article mainly focuses on simplifying the structure of interval type-2 (IT2) fuzzy system and studying its approximation properties. First of all, we construct the interval type-2 vague partitions (IT2-VPs) on input universe by overlap functions and vague partition of each attribute, and their properties are characterized. On the basis of these, we give the model of IT2 fuzzy system based on IT2-VPs, which mainly consists of the fuzzy rule base and center-of-sets type-reduction (TR). Next, we investigate its universal approximation property and approximation accuracy by error remainder term and auxiliary function method in numerical analysis. Finally, two comparative experiments indicate that the approximation performance of the proposed IT2 fuzzy systems can achieve better than the typical type-1 (T1) and IT2 fuzzy systems. [ABSTRACT FROM AUTHOR]

BACKGROUND: 2'-Fucosyllactose, a representative oligosaccharide in human milk, is an emerging and promising food and pharmaceutical ingredient due to its powerful health benefits, such as participating in immune regulation, regulation of intestinal flora, etc. To enable economically viable production of 2'-fucosyllactose, different biosynthesis strategies using precursors and pathway enzymes have been developed. The α-1,2-fucosyltransferases are an essential part involved in these strategies, but their strict substrate selectivity and unsatisfactory substrate tolerance are one of the key roadblocks limiting biosynthesis. RESULTS: To tackle this issue, a semi-rational manipulation combining computer-aided designing and screening with biochemical experiments were adopted. The mutant had a 100-fold increase in catalytic efficiency compared to the wild-type. The highest 2'-fucosyllactose yield was up to 0.65 mol mol-1 lactose with a productivity of 2.56 g mL-1 h-1 performed by enzymatic catalysis in vitro. Further analysis revealed that the interactions between the mutant and substrates were reduced. The crucial contributions of wild-type and mutant to substrate recognition ability were closely related to their distinct phylotypes in terms of amino acid preference. CONCLUSION: It is envisioned that the engineered α-1,2-fucosyltransferase could be harnessed to relieve constraints imposed on the bioproduction of 2'-fucosyllactose and lay a theoretical foundation for elucidating the substrate recognition mechanisms of fucosyltransferases. [ABSTRACT FROM AUTHOR]