Search

Objective(s): Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is an essential stage in bone formation. Autophagy plays a pivotal role in the self-renewal potential and pluripotency of stem cells. This study aimed to explore the function of autophagy-related genes during osteogenic differentiation of BMSCs. Materials and Methods: The differentially expressed autophagy-related genes (ARGs) were obtained from the GEO and HADb databases. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using R software. The PPI and hub gene mining networks were constructed using the STRING database and Cytoscape. Finally, the RT-qPCR was conducted to validate the expression level of ARGs in BMSCs. Results: Thirty-seven differentially expressed ARGs were finally obtained, including 12 upregulated and 25 downregulated genes. GO and KEGG enrichment analysis showed that most of these genes were enriched in apoptosis and autophagy. The PPI network revealed strong interactions between differentially expressed ARGs. The expression level of differentially expressed ARGs tested by RTqPCR showed 6 upregulated ARGs, including FOXO1, MAP1LC3C, CTSB, FOXO3, CALCOCO2, FKBP1A, and 4 downregulated ARGs, including MAPK8IP1, NRG1, VEGFA, and ITGA6 were consistent with the expression of high-throughput sequencing data. Conclusion: We identified 37 ARGs during osteogenic differentiation using bioinformatics analysis. FOXO1, MAP1LC3C, CTSB, FOXO3, CALCOCO2, FKBP1A, MAPK8IP1, NRG1, VEGFA, and ITGA6 may regulate osteogenic differentiation of hBMSCs by involving autophagy pathway. This study provides new insight into the osteogenic differentiation of hBMSCs and may be available in developing therapeutic strategies for maxillofacial bone defects. [ABSTRACT FROM AUTHOR]

DC arc fault introduces major safety concerns in a wide variety of components in dc networks. However, the randomness and instability of dc arc makes it difficult to be detected. In this paper, an experimental system was designed and tested at different load current, dc source voltage, and gap length to evaluate the impact of each parameter to the dc arc. Based on the experimental results, characteristics in the electrical behavior were studied and fault detection oriented analysis was conducted. A detection algorithm utilizing both time and time-frequency domain characteristics was proposed to differentiate between dc arc fault and normal condition. The detection algorithm was then realized on a digital signal processing board and tested to verify the effectiveness. Experimental results show that the proposed algorithm can detect arc fault in a timely manner and is free of nuisance trip from normal circuit operations such as load change condition. [ABSTRACT FROM AUTHOR]

Severe acute respiratory syndrome (SARS) coronavirus (SCoV) spike (S) protein is the major surface antigen of the virus and is responsible for receptor binding and the generation of neutralizing antibody. To investigate SCoV S protein, full-length and individual domains of S protein were expressed on the surface of insect cells and were characterized for cleavability and reactivity with serum samples obtained from patients during the convalescent phase of SARS. S protein could be cleaved by exogenous trypsin but not by coexpressed furin, suggesting that the protein is not normally processed during infection. Reactivity was evident by both flow cytometry and Western blot assays, but the pattern of reactivity varied according to assay and sequence of the antigen. The antibody response to SCoV S protein involves antibodies to both linear and conformational epitopes, with linear epitopes associated with the carboxyl domain and conformational epitopes associated with the amino terminal domain. Recombinant SCoV S protein appears to be a suitable antigen for the development of an efficient and sensitive diagnostic test for SARS, but our data suggest that assay format and choice of S antigen are important considerations. [ABSTRACT FROM AUTHOR]

Three kinds of forebody model of hypersonic vehicles were studied with numerical simulation method. It shows that the two-order compressive ramp model is the best selection among the three for its good evaluative parameters value at the cowl of the inlet. This model can provide higher value of flux coefficient and total pressure recovery coefficient and lower average Mach number compared with those of the other two models. Simultaneously different compressive angles may have different effects. The configuration which the first-order of compressive angle is 4° and the second 5° is the optimum combination. Furthermore factors such as attack angle were concerned. Better result may be obtained with a range of attack angles. Based on the work above the integrated design for forebody/inlet of a hypersonic vehicle was performed. The numerical result shows that this integrated model provides good flow field quality for inlet and engine work. [ABSTRACT FROM AUTHOR]

The choice of the materials used as the dielectric media in large transformers continues to be a combination of pressboard and mineral oil. However, a number of failures in the field have been attributed to the surface discharge or creeping discharge over the oil/pressboard interface. This paper reports on an experiment to study surface discharge over oil/pressboard. The TJ (triple-junction) model was used to study the characteristic of partial discharge (PD) from inception to flashover. The max PD charge, pattern, frequency and current pulse were measured during the flashover process. Four oil/pressboard specimens with different aging degrees were used and their influence on the flashover process over oil/paper interface was studied in this experiment. The results indicate that the max PD charge and PD frequency increase with the increase of applied voltage. The oil aging has great influence while the pressboard aging has little influence on PD characteristic over oil/pressboard. The PD pattern and current pulse shape show great difference for various applied voltages. The results therefore suggest that the PD detection on the oil/pressboard can be used effectively to implement the early monitor of surface discharge thereby to avoid the formation of surface flashover. [ABSTRACT FROM AUTHOR]

Background and Objectives: Peroral supplementation with trivalent-chromium (Cr) or magnesium (Mg) has been shown to improve insulin resistance (IR). The objective of this study was to determine whether combined peroral supplementation with Cr and Mg improves IR more effectively than Cr or Mg alone. Methods and Study Design: Subjects (n=120, age range 45-59 years old) and diagnosed with IR were randomly divided into four groups and monitored for a period of 3 months: group 1 (the placebo control group), group 2 (160 μg/d Cr), group 3 (200 mg/d Mg), and group 4 (160 μg/d Cr plus 200 mg/d Mg). Fasting blood glucose (FBG), fasting insulin (FIns), erythrocyte Cr and Mg content, and glucose-transporter-4 (GLUT4) and glycogen-synthase-kinase-3β (GSK3β) mRNA levels in activated T-lymphocytes were measured, and insulin resistant index (IRI) was calculated. Results: Significant decreases between the baseline and study conclusion values of FBG (0.37 mmol/L, p<0.01), FIns (2.91 μIU/mL, p<0.01) and IRI (0.60, p<0.01) were observed in group 4, but not groups 1-3. Similarly, compared with baseline, significant changes in GLUT4 (2.9-fold increase, p<0.05) and GSK3β (2.2-fold decrease, p<0.05) mRNA levels in activated T-lymphocyte were observed at the study's conclusion in group 4, but not in groups 1-3. Conclusions: Our results indicate that combining peroral supplementation with Cr and Mg improves IR more effectively than Cr or Mg alone, and this may be attributable to increased induction and repression, respectively, of GLUT4 and GSK3β expression. [ABSTRACT FROM AUTHOR]

This paper focuses on analysis and suppression of circulating harmonic currents in a modular multilevel converter (MMC) considering the impact of dead time in medium-voltage applications. A continuous equivalent model of the MMC containing two ideal transformer models is presented. Using this model, the impact of a harmonic voltage upon the dc side is analyzed and the production mechanism of circulating harmonic currents is elucidated. At the same time, the impact of dead time and insulated gate bipolar transistor (IGBT) voltage drop (DTVD) is studied, which indicates that capacitor voltages, output harmonics, and circulating harmonic currents are influenced. Based on this analysis, an open-loop control strategy to suppressing circulating harmonic currents caused by the output current and DTVD is presented. Finally, all these conclusions are verified using a simulation platform with 14 modules per arm fed by a 14-kV dc voltage source and a downscaled experimental platform with four modules per arm fed by a 560-V dc voltage source. [ABSTRACT FROM AUTHOR]

Our aim was to investigate cancer department nurses' attitudes and practices in response to pelvic radiation patients' sexual issues in Sichuan, China. A self-made questionnaire survey was conducted with 150 registered nurses of six hospitals in China. Nurses were asked about their experiences regarding consultation about sexual issues and attitudes toward the sex-related statements of pelvic radiation patients. We analyzed the correlation factors for the attitudes about dealing with patients' sexual issues by using the ff-test. Of the survey sample, 128 nurses (85.33%) responded. Of the respondents, 46.88% had been consulted about sexual issues by patients or families. In addition, 87.5% of the nurses believed that 'reliable information on sexual in pelvic radiotherapy is lacking', and 77.34% reported having 'an inter-est in undertaking education of knowledge about pelvic radiation patients' sexual issues'; yet only 4.69% had completed professional sexual education about pelvic radiation patients. The hospital type and bed number as well as nurse age and seniority also affected the responses. This study shows that discussing sexuality is still repressed in the patient-nurse rela-tionship, and most nurses' in Chinese cancer departments lack knowledge about pelvic radiation patients' sextial problems. [ABSTRACT FROM AUTHOR]

Objective: Cancer patients and their families differed in their attitude toward truth telling. The objective is to investigate different attitudes of Chinese patients or families toward whether and how to disclose diagnosis to patients with different stages of cancer and to examine the difference between the two groups. Methods: A questionnaire was delivered to 1023 participants. Results: Three hundred and eighty-two patients and 482 families completed the questionnaire. Cancer patients were more likely than families to believe that patient should be informed of the diagnosis (early-stage, 90.8 vs 69.9%, P<0.001; terminal stage, 60.5 vs 34.4%, P<0.001), and that doctor-in-charge was the appropriate person to disclose the diagnosis. Most participants thought that patient should be disclosed immediately after the diagnosis. Nearly half of participants reported that patient should be disclosed in a quiet and undisturbed room. When the hypothetic diagnosis changed from early-stage cancer to terminal illness, the number of participants, who wanted patient to know the diagnosis, decreased significantly. Conclusion: Our findings indicated that Chinese cancer patients and their families differed in their attitude toward truth telling and the attitudes toward such a disclosure were influenced by disease stage. Physicians should realize this phenomenon and pay more attention to the skills of how to disclose the cancer diagnosis. Copyright © 2007 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]