25 results on '"Xu-Hua Wang"'
Search Results
2. Complex Network Community Detection Algorithm Based on Node Similarity and Network Embedding
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YANG Xu-hua, WANG Lei, YE Lei, ZHANG Duan, ZHOU Yan-bo, LONG Hai-xia
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parameter-free community detection ,node similarity ,preference network ,network embedding ,k-means clustering ,Computer software ,QA76.75-76.765 ,Technology (General) ,T1-995 - Abstract
The community detection algorithm is very important for analyzing the topology and hierarchical structure of complex networks and predicting the evolution trend of complex networks.Traditional community detection algorithm does not have high accuracy and ignores the importance of network embedding.Aiming at such problems,a parameter-free community detection algorithm based on node similarity and network embedding Node2Vec method is proposed.First,we use the network embedding Node2Vec method to map network nodes into data points represented by low-dimensional vectors in Euclidean space,calculate the cosine similarity between the data points represented by the low-dimensional vector,construct a preference network according to the maximum similarity between the corresponding nodes,obtain the initial community detection,and use the maximum degree node of each initial community as a candidate node.Then we find the central node among the candidate nodes according to the average degree of the network and the average shortest path.Finally,the data points and their numbers corresponding to the central node are used as the initial centroid and cluster number,and the data represented by the low-dimensional vector are calculated by K-Means algorithm.The points are clustered,and the corresponding network nodes are divided into communities.This algorithm is a method of community division without parameters,which can independently extract parameters from the network without setting different hyper-parameters according to different networks,so that it can automatically and quickly identify the community structure of complex networks.In 8 real networks and artificial networks above,by comparing with other 5 well-known community discovery algorithms,numerical simulation experiments show that the proposed algorithm has good community discovery effect.
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- 2022
- Full Text
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3. Touch sensor application of spray deposited ZnO films.
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Shrawan Jha, Xu-Hua Wang, and Hendrik Faber
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- 2017
- Full Text
- View/download PDF
4. Genome-wide identification and analysis of basic helix-loop-helix domains in dog, Canis lupus familiaris
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Xiao-Ting Liu, Keping Chen, Yang Zhou, Qin Yao, A-Ke Liu, Xu-Hua Wang, and Yong Wang
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endocrine system ,Sequence analysis ,Molecular Sequence Data ,Biology ,Genome ,Conserved sequence ,Dogs ,Phylogenetics ,Basic Helix-Loop-Helix Transcription Factors ,Genetics ,Animals ,media_common.cataloged_instance ,Amino Acid Sequence ,Molecular Biology ,Gene ,Conserved Sequence ,Phylogeny ,media_common ,Phylogenetic tree ,Helix-Loop-Helix Motifs ,Chromosome Mapping ,Molecular Sequence Annotation ,Sequence Analysis, DNA ,General Medicine ,Introns ,Protein Structure, Tertiary ,Canis lupus familiaris ,GenBank - Abstract
The basic helix-loop-helix (bHLH) domain is a highly conserved amino acid motif that defines a group of DNA-binding transcription factors. bHLH proteins play essential regulatory roles in a variety of biological processes in animal, plant, and fungus. The domestic dog, Canis lupus familiaris, is a good model organism for genetic, physiological, and behavioral studies. In this study, we identified 115 putative bHLH genes in the dog genome. Based on a phylogenetic analysis, 51, 26, 14, 4, 12, and 4 dog bHLH genes were assigned to six separate groups (A-F); four bHLH genes were categorized as ''orphans''. Within-group evolutionary relationships inferred from the phylogenetic analysis were consistent with positional conservation, other conserved domains flanking the bHLH motif, and highly conserved intron/exon patterns in other vertebrates. Our analytical results confirmed the GenBank annotations of 89 dog bHLH proteins and provided information that could be used to update the annotations of the remaining 26 dog bHLH proteins. These data will provide good references for further studies on the structures and regulatory functions of bHLH proteins in the growth and development of dogs, which may help in understanding the mechanisms that underlie the physical and behavioral differences between dogs and wolves.
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- 2014
5. A genome-wide identification and classification of basic helix-loop-helix genes in the jewel wasp,Nasonia vitripennis(Hymenoptera: Pteromalidae)
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Xu-Hua Wang, Xia-Fang Tao, Qin Yao, Yong Wang, Xiao-Ting Liu, and Keping Chen
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Genetics ,biology ,Basic helix-loop-helix ,Genome, Insect ,Helix-Loop-Helix Motifs ,Wasps ,Genes, Insect ,SUPERFAMILY ,General Medicine ,Hymenoptera ,biology.organism_classification ,Genome ,Nasonia vitripennis ,Multigene Family ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,Insect Proteins ,Pteromalidae ,Molecular Biology ,Gene ,Transcription factor ,Biotechnology - Abstract
Basic helix-loop-helix (bHLH) proteins are highly conserved DNA-binding transcription factors of a large superfamily. Animal bHLH proteins play important regulatory roles in various developmental processes such as neurogenesis, myogenesis, heart development, and hematopoiesis. The jewel wasp (Nasonia vitripennis) is a good model organism of hymenoptera insects for studies of developmental and evolutionary genetics. In this study, we identified 48 bHLH genes in the genome of N. vitripennis. According to phylogenetic analysis, based on N. vitripennis bHLH (NvbHLH) motif sequences and structural domain distribution in their full-length protein sequences, the identified NvbHLH genes were classified into 36 bHLH families with 19, 12, 9, 1, 6, and 1 member(s) in groups A, B, C, D, E, and F, respectively. Our classification to the identified NvbHLH family members confirms GenBank annotations for 21 of the 48 NvbHLH proteins and provides useful information for further characterization and annotation of the remaining 27 NvbHLH proteins. Compared to other insect species, N. vitripennis has the lowest number of bHLH family members. No NvbHLH members have been found in the families Net, MyoRa, and PTFa, while all other insect species have at least one member in each of the families. These data constitute a solid basis for further investigations into the functions of bHLH proteins in developmental regulation of N. vitripennis.
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- 2014
6. Touch sensor application of spray deposited ZnO films
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Xu-Hua Wang, Hendrik Faber, and S. K. Jha
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010302 applied physics ,Materials science ,business.industry ,chemistry.chemical_element ,Optical transmittance ,02 engineering and technology ,Zinc ,021001 nanoscience & nanotechnology ,01 natural sciences ,Indium tin oxide ,chemistry ,0103 physical sciences ,Electrode ,Optoelectronics ,0210 nano-technology ,business ,Sheet resistance - Abstract
We demonstrate touch sensor application of spray coated Zinc Oxide (ZnO) thin-films with submicron thickness (50–200 nm). The prepared ZnO films are characteristic with a wide optical transmittance window (80% ∼ 97%) and a sheet resistance over 1500 ohm/square. The touch sensor was formed using a ZnO thin-film as an electrode attached to an oscillator circuit. The prepared touch sensors produce a significant change of the oscillation frequency in response to touch by human figure to ZnO electrodes. Thus feasibility of touch sensor application of spray deposited ZnO films is demonstrated. Thus, ZnO films may have an advantage over the conventional ITO electrodes due to a low cost and large area manufacturing by spray-coating technique.
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- 2017
7. Research of Zero Correlation Zone Sequence Pairs Set Construction Method Based on Interleaving Technique
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Wei-dong Peng, Ming-yang Li, Xu-hua Wang, Feng Wang, and Peng Bai
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Discrete mathematics ,Set (abstract data type) ,Sequence ,Construction method ,Interleaving ,Zero correlation ,Electrical and Electronic Engineering ,Mathematics - Published
- 2014
8. A Precise Synchronization Method Based on Iterative Least Square Algorithm
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Wei-dong Peng, Hu Lu, Jin-fu Lin, Ming-yang Li, Xu-hua Wang, and Peng Bai
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Least mean square algorithm ,Mathematical optimization ,Computer science ,Iterative method ,Synchronization (computer science) ,Electrical and Electronic Engineering ,Algorithm - Published
- 2014
9. A TRI-BAND ELECTROMAGNETIC ABSORBER WITH INSENSITIVE PROPERTIES
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Mingbao Yan, Na Fu, Hang Zhou, Xu-Hua Wang, Xin-Hua Wang, and Ming-Yang Li
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Materials science ,Optics ,business.industry ,Rotational symmetry ,Metamaterial absorber ,Optoelectronics ,business ,Absorption (electromagnetic radiation) ,High absorption ,Electronic, Optical and Magnetic Materials - Abstract
In this paper, we present a tri-band electromagnetic absorber with insensitive properties. A rotational symmetry structure with a metallic ground is proposed for the design of the metamaterial absorber. Calculation results show that the absorber has three perfect absorption points at 4.76 GHz, 7.61 GHz and 10.84 GHz with the corresponding absorption rates of 96.7%, 97.8%, and 99.3%. An experiment is given, and the results verify our design. Such a tri-band absorber has the merits of high absorption rate, stable performance with various incident angles and different polarizations.
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- 2014
10. Phylogenetic analyses of vector mosquito basic helix-loop-helix transcription factors
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Debao Zhang, Qin Yao, Xu-Hua Wang, Yong Wang, C. W. Dang, A-Ke Liu, and Kangmin Chen
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Genetics ,biology ,Phylogenetic tree ,Anopheles gambiae ,fungi ,Intron ,Aedes aegypti ,biology.organism_classification ,Genome ,OrthoDB ,Insect Science ,parasitic diseases ,KEGG ,Molecular Biology ,Gene - Abstract
Basic helix-loop-helix (bHLH) transcription factors play critical roles in the regulation of a wide range of developmental processes in higher organisms and have been identified in more than 20 organisms. Mosquitoes are important vectors of certain human diseases. In this study, Aedes aegypti, Anopheles gambiae str. PEST and Culex quinquefasciatus genomes were found to encode 55, 55 and 57 bHLH genes, respectively. Further phylogenetic analyses and OrthoDB and Kyoto encyclopedia of genes and genomes orthology database searches led us to define orthology for all the identified mosquito bHLHs successfully. This provides useful information with which to update annotations to 40 Ae. aegypti, 55 An. gambiae and 38 C. quinquefasciatus bHLH genes in VectorBase. The mosquito lineage has more bHLH genes in the Atonal, neurogenin (Ngn) and Hes-related with YRPW motif (Hey) families than do other insect species, suggesting that mosquitoes have evolved to be more sensitive to vibration, light and chemicals. Mosquito bHLH genes generally have higher evolutionary rates than other insect species. However, no pervasive positive selection occurred in the evolution of insect bHLH genes. Only episodic positive selection was found to affect evolution of bHLH genes in 11 families. Besides, coding regions of several Ae. aegypti bHLH motifs have unusually long introns in which multiple copies of transposable elements have been identified. These data provide a solid basis for further studies on structures and functions of bHLH proteins in the regulation of mosquito development and for prevention and control of mosquito-mediated human diseases.
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- 2013
11. Anti-frequency Offset Method for Precision Synchronization on Highly Dynamic Condition Based on the Least Squares
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Huan-yu Li, Xu-hua Wang, Meng-da Li, Peng Bai, and Ming-yang Li
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Control theory ,Computer science ,Synchronization (computer science) ,Frequency offset ,Electrical and Electronic Engineering ,Least squares - Published
- 2013
12. DUAL-BAND FREQUENCY SELECTIVE SURFACE WITH MINIATURIZED ELEMENT IN LOW FREQUENCIES
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Yuan Yang, Xu-Hua Wang, and Hang Zhou
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Engineering ,business.industry ,Acoustics ,Electronic engineering ,Tunable metamaterials ,Multi-band device ,Element (category theory) ,Concentric ,business ,Passband ,Square (algebra) ,Electronic, Optical and Magnetic Materials - Abstract
In this paper, we propose a dual-band frequency selective surface (FSS) in low frequencies with miniaturized element. A dual- concentric square element with two difierent slot sizes is constructed to realize dual-band passband responses. Each passband is realized by a square slot structure. Besides, we reduce the slot sizes to make the element miniature and compact. Based on this technique, a dual- band FSS with miniaturized element in low frequencies is designed. Both the simulation and experiment results show that such a FSS owes its advantages to miniature element, stable performance with various incident angles and difierent polarizations, which is suitable for dual- band shipboard communication.
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- 2012
13. Effect of β-escin sodium on endothelial cells proliferation, migration and apoptosis
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Jing-Tao Liu, Bo Xu, Xu-Hua Wang, and Jing-Rong Cui
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Protein Kinase C-alpha ,Physiology ,Angiogenesis ,Sodium ,Blotting, Western ,Motility ,chemistry.chemical_element ,Apoptosis ,Biology ,p38 Mitogen-Activated Protein Kinases ,Umbilical vein ,Cell Line ,Thrombospondin 1 ,Cell Movement ,Humans ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase A ,Cells, Cultured ,Cell Proliferation ,Pharmacology ,Analysis of Variance ,Escin ,Dose-Response Relationship, Drug ,Cell growth ,Endothelial Cells ,Cell migration ,Saponins ,Flow Cytometry ,Molecular biology ,Biochemistry ,chemistry ,Molecular Medicine - Abstract
beta-Escin, the major active compound in extracts of the horse chestnut Aesculus hippocastanum seed, has shown clinically significant activity in chronic venous insufficiency (CVI). Our previous studies had shown that beta-escin sodium inhibited angiogenesis in chick chorioallantoic membrane (CAM) and in aortic disk assay. In this study, we explored the direct effect of beta-escin sodium on proliferation, migration and apoptosis in human umbilical vein endothelial cells (HUVECs) and ECV304 cells. Sulforhodamine B (SRB) assay showed that beta-escin sodium (10, 20, 40 microg/ml) inhibited endothelial cells (ECs) proliferation dose-dependently. beta-escin sodium also induced ECs apoptosis at 40 microg/ml. Cell migration was evaluated by an improved wound assay: barren spot assay. And the direct effect on cell motility excluding influence of cell proliferation was examined by High Content Screening (HCS, Cellomics) assay. The data indicated that beta-escin sodium suppressed ECs migration and cell motility. Western blot results suggested that beta-escin sodium acts on ECs possibly by increasing expression of thrombospondin-1 (TSP-1), and decreasing expression of PKC-alpha and activation of p44/42 mitogen-activated protein kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK). Our findings give the evidence that beta-escin sodium might have potential anti-angiogenic activity via its direct effects on ECs.
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- 2008
14. A Genome-Wide Identification of Basic Helix-Loop-Helix Motifs in Pediculus humanus corporis (Phthiraptera: Pediculidae)
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A-Ke Liu, Keping Chen, Qin Yao, Xu-Hua Wang, Debao Zhang, and Yong Wang
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Hypothetical protein ,Molecular Sequence Data ,BLAST search ,Louse ,Genome ,Phylogenetics ,biology.animal ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,Human Body Louse ,Amino Acid Sequence ,Pediculus humanus corporis ,Gene ,Phylogeny ,Whole genome sequencing ,Genetics ,biology ,Research ,phylogenetic analysis ,Helix-Loop-Helix Motifs ,Pediculus ,Chromosome Mapping ,General Medicine ,ortholog ,Insect Science ,GenBank ,basic helix-loop-helix ,Insect Proteins ,Genome-Wide Association Study - Abstract
Basic helix-loop-helix (bHLH) proteins comprise a large superfamily of transcription factors, which are involved in the regulation of various developmental processes. bHLH family members are widely distributed in various eukaryotes including yeast, fruit fly, zebrafish, mouse, and human. In this study, we identified 55 bHLH motifs encoded in genome sequence of the human body louse, Pediculus humanus corporis (Phthiraptera: Pediculidae). Phylogenetic analyses of the identified P. humanus corporis bHLH (PhcbHLH) motifs revealed that there are 23, 11, 9, 1, 10, and 1 member(s) in groups A, B, C, D, E, and F, respectively. Examination to GenBank annotations of the 55 PhcbHLH members indicated that 29 PhcbHLH proteins were annotated in consistence with our analytical result, 8 were annotated different with our analytical result, 12 were merely annotated as hypothetical protein, and the rest 6 were not deposited in GenBank. A comparison on insect bHLH gene composition revealed that human body louse possibly has more hairy and E(spl) genes than other insect species. Because hairy and E(spl) genes have been found to negatively regulate the differentiation of insect preneural cells, it is suggested that the existence of additional hairy and E(spl) genes in human body louse is probably the consequence of its long period adaptation to the relatively dark and stable environment. These data provide good references for further studies on regulatory functions of bHLH proteins in the growth and development of human body louse.
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- 2014
15. Classification and evolutionary analysis of the basic helix-loop-helix gene family in the green anole lizard, Anolis carolinensis
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Qin Yao, Keping Chen, A-Ke Liu, Yong Wang, Debao Zhang, Xu-Hua Wang, Huifang Song, and Chunwang Dang
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Amino Acid Motifs ,Biology ,Genome ,Anolis ,Conserved sequence ,Evolution, Molecular ,Mice ,Open Reading Frames ,Phylogenetics ,Genetics ,Basic Helix-Loop-Helix Transcription Factors ,Gene family ,Animals ,Position-Specific Scoring Matrices ,Molecular Biology ,Gene ,Conserved Sequence ,Phylogeny ,Phylogenetic tree ,Helix-Loop-Helix Motifs ,Intron ,Computational Biology ,Lizards ,General Medicine ,Exons ,biology.organism_classification ,Introns ,Multigene Family - Abstract
Helix-loop-helix (bHLH) proteins play essential regulatory roles in a variety of biological processes. These highly conserved proteins form a large transcription factor superfamily, and are commonly identified in large numbers within animal, plant, and fungal genomes. The bHLH domain has been well studied in many animal species, but has not yet been characterized in non-avian reptiles. In this study, we identified 102 putative bHLH genes in the genome of the green anole lizard, Anolis carolinensis. Based on phylogenetic analysis, these genes were classified into 43 families, with 43, 24, 16, 3, 10, and 3 members assigned into groups A, B, C, D, E, and F, respectively, and 3 members categorized as "orphans". Within-group evolutionary relationships inferred from the phylogenetic analysis were consistent with highly conserved patterns observed for introns and additional domains. Results from phylogenetic analysis of the H/E(spl) family suggest that genome and tandem gene duplications have contributed to this family's expansion. Our classification and evolutionary analysis has provided insights into the evolutionary diversification of animal bHLH genes, and should aid future studies on bHLH protein regulation of key growth and developmental processes.
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- 2013
16. [Dynamic change of enzyme activity of patients with acute endosulfan poisoning and clinical significance]
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Xu-Hua, Wang, Cui-Juan, Wang, and Lin, Li
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Adult ,Male ,Electrocardiography ,Young Adult ,Adolescent ,Case-Control Studies ,Humans ,Female ,Aspartate Aminotransferases ,Middle Aged ,Lactate Dehydrogenases ,Endosulfan ,Aged - Published
- 2010
17. [Determination of myocardial enzymes to assess acute CO poisoning-induced myocardial damage]
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Xu-Hua, Wang and Ling-Ling, Hu
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Adult ,Aged, 80 and over ,Male ,Carbon Monoxide Poisoning ,Young Adult ,Adolescent ,Case-Control Studies ,Myocardium ,Humans ,Female ,Middle Aged ,Aged - Published
- 2005
18. L-type calcium channel blockers enhance 5-HTP-induced antinociception in mice
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Jian-hui, Liang, Jun-xu, Li, Xu-hua, Wang, Bi, Chen, Ying, Lu, Pan, Zhang, Rong, Han, and Xiang-feng, Ye
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Analgesics ,Calcium Channels, L-Type ,Nifedipine ,Drug Synergism ,Motor Activity ,Calcium Channel Blockers ,5-Hydroxytryptophan ,Calcium Chloride ,Mice ,Pargyline ,Verapamil ,Fluoxetine ,Animals ,Female ,Nimodipine ,Egtazic Acid ,Selective Serotonin Reuptake Inhibitors ,Chelating Agents ,Injections, Intraventricular ,Pain Measurement - Abstract
To investigate the involvement of L-type Ca(2+) channels in antinociceptive action induced by the 5-HT precursor, 5-hydroxytryptophan (5-HTP).Female Kunming mice were treated with either 5-HTP (20-80 mg/kg, ip) alone, or the combination of 5-HTP and fluoxetine (2-8 mg/kg, ip), pargyline (15-60 mg/kg, ip), nimodipine (2.5-10 mg/kg, ip), nifedipine (2.5-10 mg/kg, ip), verapamil (2.5-10 mg/kg, ip), CaCl(2) (5-20 mmol/L, icv), or EGTA (0.5-3 mmol/L, icv) prior to the hot-plate test (55 degree, hind-paw licking latency). In addition, locomotor activity in mice treated with 5-HTP alone was measured using an ambulometer with five activity boxes.Ip injection of 5-HTP alone had no influence on the spontaneous locomotor activity, whereas dose-dependently increased the latency to licking hind-paw in the hot-plate test in mice. The inhibitory effects of 5-HTP on nociceptive response were significantly enhanced by fluoxetine in the mouse hot-plate test. At a sub-effective dose, pargyline could cause a leftward shift in the dose-response curve of 5-HTP-induced antinociception. Co-administration with 5-HTP and nimodipine, nifedipine, or verapamil obviously potentiated the antinociceptive effects elicited by 5-HTP. Interestingly, 5-HTP-induced antinociception was antagonized by CaCl(2) and enhanced by EGTA injected icv in the mouse hot-plate test.These findings suggest that systemic administration of 5-HTP may yield the antinociceptive effects, which are related to Ca(2+) influx from extracellular fluid through L-type Ca(2+) channels.
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- 2004
19. Buspirone-induced antinociception is mediated by L-type calcium channels and calcium/caffeine-sensitive pools in mice
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Hong-Lei Sun, Xiang-Feng Ye, R Liu, Xu-Hua Wang, Ji-Wang Zheng, and Jian-Hui Liang
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Agonist ,medicine.medical_specialty ,Calcium Channels, L-Type ,Nifedipine ,medicine.drug_class ,Calcium channel blocker ,Partial agonist ,Buspirone ,5-Hydroxytryptophan ,Calcium Chloride ,Mice ,Internal medicine ,Caffeine ,Fluoxetine ,medicine ,Reaction Time ,Animals ,L-type calcium channel ,Egtazic Acid ,Chelating Agents ,Injections, Intraventricular ,Pain Measurement ,Pharmacology ,Analgesics ,Voltage-dependent calcium channel ,Ryanodine receptor ,Chemistry ,Ryanodine ,Calcium Channel Blockers ,Serotonin Receptor Agonists ,Endocrinology ,Receptors, Serotonin ,Second messenger system ,Calcium ,Central Nervous System Stimulants ,Female ,Receptors, Serotonin, 5-HT1 ,Selective Serotonin Reuptake Inhibitors ,medicine.drug - Abstract
Previous studies have shown that buspirone, a partial 5-HT(1A) receptor agonist, produces antinociceptive effects in rats and mice; Ca(2+) plays a critical role as a second messenger in mediating nociceptive transmission. 5-HT(1A) receptors have been proven to be coupled functionally with various types of Ca(2+) channels in neurons, including N-, P/Q-, T-, or L-type. It was of interest to investigate the involvement of extracellular/intracellular Ca(2+) in buspirone-induced antinociception.To determine whether central serotonergic pathways participate in the antinociceptive processes of buspirone, and investigate the involvement of Ca(2+) mechanisms, particularly L-voltage-gated Ca(2+) channels and Ca(2+)/caffeine-sensitive pools, in buspirone-induced antinociception.Antinociception was assessed using the hot-plate test (55 degrees C, hind-paw licking latency) in mice treated with either buspirone (1.25-20 mg/kg i.p.) alone or the combination of buspirone and fluoxetine (2.5-10 mg/kg i.p.), 5-HTP (25 mg/kg i.p.), nimodipine (2.5-10 mg/kg i.p.), nifedipine (2.5-10 mg/kg i.p.), CaCl(2) (25-200 nmol per mouse i.c.v.), EGTA (5-30 nmol per mouse i.c.v.), or ryanodine (0.25-2 nmol per mouse i.c.v.).Buspirone dose dependently increased the licking latency in the hot-plate test in mice. This effect of buspirone was enhanced by fluoxetine, 5-HTP, nimodipine, and nifedipine. Interestingly, central administration of Ca(2+) reversed the antinociceptive effects of buspirone. In contrast to these, ryanodine or EGTA administered centrally potentiated buspirone-induced antinociception.Decreasing neuronal Ca(2+) levels potentiated buspirone-induced antinociception; conversely, increasing intracellular Ca(2+) abolished the antinociceptive effects of buspirone. These results suggest that Ca(2+) influx from extracellular fluid and release of Ca(2+) from Ca(2+)/caffeine-sensitive microsomal pools may be involved in buspirone-induced antinociception.
- Published
- 2002
20. The expression of c-src gene in the carcinogenesis process of human cardia adenocarcinoma
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Lin Xiao, Xu-Hua Wang, Shu-Lan Yuan, Chao-Jun Wang, and Xiu-Jia Wang
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Pathology ,medicine.medical_specialty ,medicine.drug_class ,Gastroenterology ,General Medicine ,Original Articles ,Biology ,medicine.disease ,medicine.disease_cause ,Monoclonal antibody ,digestive system ,digestive system diseases ,medicine.anatomical_structure ,medicine ,Carcinoma ,otorhinolaryngologic diseases ,Immunohistochemistry ,Adenocarcinoma ,Carcinogenesis ,Gene ,Lymph node ,Proto-oncogene tyrosine-protein kinase Src - Abstract
AIM:To investigate the activation, expression of c-src gene and its role in the carcinogenetic process of human cardia adenocarcinoma (CA).METHODS:Fifty six cases of CA, 34 cases of normal, 36 cases of protiferative epithelia adjacent to carcinoma, and 20 cases of lymph node metastases of CA were studied for PP60(c-src),the expression product of c-src gene immunohistochemically by using the specific monoclonal antibody,Mab327.RESULTS: The positive rates of PP60(c-src) in the normal epithelia,protiferative epithelia, CA and lymph node metastases were 29.4% (10/34), 94.4% (34/36), 71.4% (40/56) and 60.0%(12/20), respectively, among them, the differences of the positive rates were statistically significant (P < 0.01). The expression levels of PP60(c-src) in CA and proli ferative epithelia were significantly higher than that in the normal epithelia(P< 0.01).The PP60(c-src) positive rates in the papillary, tubular, poorly different-tiated and mucous adenocarcinoma were 75.0% (6/8), 81.8% (18/22), 50.0% (10/20) and 100.0% (6/6), respectively, whereas those of tubular and mucous adenocarcinomas were significantly higher than those of papillary and poorly differentiated adenocar-cinomas (P < 0.05), and the PP60(c-src) expression levels of tubular and mucous adenocarcinomas were also significantly higher than those of papillary and poorly differentiated adenocarcinomas (P< 0.01).CONCLUSION:The activation and expression of c-src gene are associated with the initiation and development of human CA; the protein amount of PP60(c-src)increased during the process of carcinogenesis; and PP60(c-src) expression is also related to lymph node metastases.
- Published
- 2002
21. Non-Traditional CD4+ CD25–CD69+ Regulatory T Cells Is Correlated To Leukemia Relapse After Allogeneic Hematopoietic Stem Cell Transplantation
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Xiao-Jun Huang, Lan-Ping Xu, Xiao-Su Zhao, Xu-hua Wang, Kai-Yan Liu, Ying-Jun Chang, Xiao-Hui Zhang, Xiang-Yu Zhao, and Dai-Hong Liu
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business.industry ,medicine.medical_treatment ,T cell ,Immunology ,chemical and pharmacologic phenomena ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,Immunotherapy ,medicine.disease ,Biochemistry ,Minimal residual disease ,Leukemia ,medicine.anatomical_structure ,Immune system ,Medicine ,Cytokine secretion ,Bone marrow ,business - Abstract
Introduction Leukemia relapse is the most common cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The biological mechanism of relapse is still not completely clear. Allo-HSCT can be considered as a kind of immunotherapy directed toward malignant hematologic disease. Confirmation the correlation between clinical relapse and specific subset of T cells and further elucidating the immune mechanism behind this phenomenon would be beneficial to explore new methods of adoptive immunotherapy. Accumulative evidence showed that regulatory T cells (Tregs) might be involved in immune escape mechanism and associated with the failure of host to trigger efficient immunological antitumor response. Recently, a kind of non-traditional CD4+ CD25–CD69+ T cells was found to be involved in disease progression in tumor-bearing mouse models and cancer patients. In this study, we attempted to define whether this subset of T cells was related to leukemia relapse after allo-HSCT and also demonstrate the potential immune regulatory mechanism behind this phenomenon. Methods Twenty-nine patients with malignant hematological disease treated with non-T-cell-depleted allo-HSCT at the Peking University Institute of Hematology from November 2009 to April 2011 including patients undergoing hematological relapse (n=22) and with detectable minimal residual disease (MRD, n=7) were selected as the initial subjects in this study. The other fifty-six patients who received allo-HSCT from October 2009 to July 2010 were also enrolled for prospective study. The bone marrow of the initial 29 subjects was collected at the time of hematological relapse or detecting MRD. The MRD status of those 56 patients was examined at regular time points: +30 day (d), +60d, +90d, +180d, +270d, +360d. Bone marrow samples from patients at above time points were collected for the MRD examination and counting of CD4+CD25-CD69+ cells by flow cytometry (FCM). Results The frequency of CD4+CD25-CD69+ T cells in healthy donors’ bone marrow was 2.79% (range, 2.11-4.94%). However, the frequency of this subset of T cell was significantly increased in patients with detectable MRD (7.60%, range, 4.53-9.14%, P=0.008), or undergoing hematological relapse (12.96%, range, 8.62-20.49%, P0.05). The incidence of MRD+ or relapse in high frequency of CD4+CD25-CD69+ T cells group (>7%) was distinctly higher than that of low frequency of CD4+CD25-CD69+ T cells group at +60d, +90d and +270d after transplant. However, our preliminary data indicated that CD4+CD25-CD69+ T cells might not display immune regulatory function via cytokine secretion. Conclusions This study gave the first clinical evidence of correlation between non-traditional CD4+CD25-CD69+ T cells and leukemia relapse after allo-HSCT and would be beneficial to explore new methods of adoptive immunotherapy. Further research related to regulatory mechanism behind this phenomenon would be necessary. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2013
22. A Tri-Band Electromagnetic Absorber with Insensitive Properties.
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Xu-Hua Wang, Hang Zhou, Ming-Bao Yan, Na Fu, Ming-Yang Li, and Xin-Hua Wang
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ELECTROMAGNETISM ,ELECTROMAGNETIC wave absorption ,ROTATIONAL symmetry ,METAMATERIALS ,POLARIZATION (Electricity) - Abstract
In this paper, we present a tri-band electromagnetic absorber with insensitive properties. A rotational symmetry structure with a metallic ground is proposed for the design of the metamaterial absorber. Calculation results show that the absorber has three perfect absorption points at 4.76 GHz, 7.61 GHz and 10.84 GHz with the corresponding absorption rates of 96.7%, 97.8%, and 99.3%. An experiment is given, and the results verify our design. Such a tri-band absorber has the merits of high absorption rate, stable performance with various incident angles and different polarizations. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
23. Non-traditional CD4+CD25-CD69+ regulatory T cells are correlated to leukemia relapse after allogeneic hematopoietic stem cell transplantation.
- Author
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Xiao-su Zhao, Xu-hua Wang, Xiang-yu Zhao, Ying-jun Chang, Lan-ping Xu, Xiao-hui Zhang, and Xiao-jun Huang
- Subjects
- *
T cells , *LEUKEMIA , *HEMATOPOIETIC stem cell transplantation , *DISEASE relapse , *CYTOKINES - Abstract
Background Non-traditional CD4+CD25-CD69+ T cells were found to be involved in disease progression in tumor-bearing mouse models and cancer patients recently. We attempted to define whether this subset of T cells was related to leukemia relapse after allogeneic hematopoietic cell transplantation (allo-HSCT). Methods The frequency of CD4+CD25-CD69+ T cells among the CD4+ T cell population from the bone marrow of relapsed patients, patients with positive minimal residual disease (MRD+) and healthy donors was examined by flow cytometry. The CD4+CD25-CD69+ T cells were also stained with the intracellular markers to determine the cytokine (TGF-β, IL-2 and IL-10) secretion. Results The results showed that the frequency of CD4+CD25-CD69 + T cells was markedly increased in patients in the relapsed group and the MRD + group compared to the healthy donor group. The percentage of this subset of T cells was significantly decreased after effective intervention treatment. We also analyzed the reconstitution of CD4+CD25-CD69+ T cells at various time points after allo-HSCT, and the results showed that this subset of T cells reconstituted rapidly and reached a relatively higher level at +60 d in patients compared to controls. The incidence of either MRD or relapse in patients with a high frequency of CD4+CD25-CD69+ T cells (>7%) was significantly higher than that of patients with a low frequency of CD4+CD25-CD69+ T cells at +60 d, +90 d and +270 d after transplant. However, our preliminary data indicated that CD4+CD25-CD69+ T cells may not exert immunoregulatory function via cytokine secretion. Conclusions This study provides the first clinical evidence of a correlation between non-traditional CD4+CD25-CD69+ Tregs and leukemia relapse after allo-HSCT and suggests that exploration of new methods of adoptive immunotherapy may be beneficial. Further research related to regulatory mechanism behind this phenomenon would be necessary. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
24. A Genome-Wide Identification of Basic Helix-Loop-Helix Motifs in Pediculus humanus corporis (Phthiraptera: Pediculidae).
- Author
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Xu-Hua Wang, Yong Wang, De-Bao Zhang, A-Ke Liu, Qin Yao, and Ke-Ping Chen
- Abstract
Basic helix-loop-helix (bHLH) proteins comprise a large superfamily of transcription factors, which are involved in the regulation of various developmental processes. bHLH family members are widely distributed in various eukaryotes including yeast, fruit fly, zebrafish, mouse, and human. In this study, we identified 55 bHLH motifs encoded in genome sequence of the human body louse, Pediculus humanus corporis (Phthiraptera: Pediculidae). Phylogenetic analyses of the identified P. humanus corporis bHLH (PhcbHLH) motifs revealed that there are 23, 11, 9, 1, 10, and 1 member(s) in groups A, B, C, D, E, and F, respectively. Examination to GenBank annotations of the 55 PhcbHLH members indicated that 29 PhcbHLH proteins were annotated in consistence with our analytical result, 8 were annotated different with our analytical result, 12 were merely annotated as hypothetical protein, and the rest 6 were not deposited in GenBank. A comparison on insect bHLH gene composition revealed that human body louse possibly has more hairy and E(spl) genes than other insect species. Because hairy and E(spl) genes have been found to negatively regulate the differentiation of insect preneural cells, it is suggested that the existence of additional hairy and E(spl) genes in human body louse is probably the consequence of its long period adaptation to the relatively dark and stable environment. These data provide good references for further studies on regulatory functions of bHLH proteins in the growth and development of human body louse. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
25. Non-traditional CD4+CD25−CD69+ regulatory T cells are correlated to leukemia relapse after allogeneic hematopoietic stem cell transplantation
- Author
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Xiang-Yu Zhao, Lan-Ping Xu, Xiao-Hui Zhang, Xiao-Jun Huang, Ying-Jun Chang, Xiao-Su Zhao, and Xu-hua Wang
- Subjects
Antigens, Differentiation, T-Lymphocyte ,Male ,Neoplasm, Residual ,medicine.medical_treatment ,Immunoregulatory ,Hematopoietic stem cell transplantation ,Lymphocyte Activation ,T-Lymphocytes, Regulatory ,Mice ,immune system diseases ,Bone Marrow ,Relapse ,Child ,Medicine(all) ,Leukemia ,Hematopoietic Stem Cell Transplantation ,hemic and immune systems ,General Medicine ,Regulatory T cells ,Middle Aged ,medicine.anatomical_structure ,CD4 Antigens ,Cytokines ,Female ,Adult ,Adolescent ,T cell ,Allogeneic Hematopoietic Stem Cell Transplantation ,chemical and pharmacologic phenomena ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Immunomodulation ,Antigen ,Antigens, CD ,medicine ,Animals ,Humans ,Transplantation, Homologous ,Lectins, C-Type ,Lymphocyte Count ,Biochemistry, Genetics and Molecular Biology(all) ,Research ,Interleukin-2 Receptor alpha Subunit ,medicine.disease ,Minimal residual disease ,Transplantation ,Immunology ,Cytokine secretion ,Bone marrow ,Neoplasm Recurrence, Local - Abstract
Background Non-traditional CD4+CD25–CD69+ T cells were found to be involved in disease progression in tumor-bearing mouse models and cancer patients recently. We attempted to define whether this subset of T cells were related to leukemia relapse after allogeneic hematopoietic cell transplantation (allo-HSCT). Methods The frequency of CD4+CD25–CD69+ T cells among the CD4+ T cell population from the bone marrow of relapsed patients, patients with positive minimal residual disease (MRD+) and healthy donors was examined by flow cytometry. The CD4+CD25-CD69+ T cells were also stained with the intracellular markers to determine the cytokine (TGF-β, IL-2 and IL-10) secretion. Results The results showed that the frequency of CD4+CD25–CD69 + T cells was markedly increased in patients in the relapsed group and the MRD + group compared to the healthy donor group. The percentage of this subset of T cells was significantly decreased after effective intervention treatment. We also analyzed the reconstitution of CD4+CD25–CD69+ T cells at various time points after allo-HSCT, and the results showed that this subset of T cells reconstituted rapidly and reached a relatively higher level at +60 d in patients compared to controls. The incidence of either MRD+ or relapse in patients with a high frequency of CD4+CD25-CD69+ T cells (>7%) was significantly higher than that of patients with a low frequency of CD4+CD25-CD69+ T cells at +60 d, +90 d and +270 d after transplant. However, our preliminary data indicated that CD4+CD25-CD69+ T cells may not exert immunoregulatory function via cytokine secretion. Conclusions This study provides the first clinical evidence of a correlation between non-traditional CD4+CD25-CD69+ Tregs and leukemia relapse after allo-HSCT and suggests that exploration of new methods of adoptive immunotherapy may be beneficial. Further research related to regulatory mechanism behind this phenomenon would be necessary.
- Full Text
- View/download PDF
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