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Additionalfile 2: Figure S1. Correlation analysis of shoot and root samples. HIR:High Iron Root, HIS: High Iron Shoot, LIR: Low Iron Root, LIS: Low Iron Shoot.1, 2, and 3 respectively represent different biological repetitions. Figure S2. Genomic distribution ofdifferentially expressed genes (DEGs). Genomic distribution of DEGs in the shoots (A)and roots (B). Figure S3. Molecularmodel of Fe absorption and transport related genes. A molecular model of genesinvolved in Fe absorption and transport in plant roots (A), chloroplasts,mitochondria, and vacuoles (B). FigureS4. Differential expression profiles of genes related to Fe absorption andtransport in wheat plants under low Fe stress. (A) Fe3+ chelatereductase (FRO), (B) naturalresistance-associated macrophage protein (NRAPM),(C) Yellow stripe-like (YSL), (D) methionine synthetase (SAM), (E) iron-regulated transporter (IRT), (F) Deoxyergate synthase (DMAS), (G) Nicotinamide aminotransferase(NAAT), (H) Mitochondrial m-typethioredoxin in chloroplast (ATM), (I)Nicotinamide synthase (NAS), (J)transporter of mugnetic acid (TOM),(K) multidrug and toxin efflux family (MATE),(L) Permease in chioroplasts (PIC),(M) Oligopeptide transporter (OPT),(N) mitoferrin, (O) mitoferrin-like, (P) Iron efflux transporter ferroportin (FPN), (Q) Nonintrinsic ABC protein (NAP), (R) vacuolar iron transporterginseng (VIT). For transcriptomesequencing, selected uniform wheat plants after germination, half of which weretransplanted into a nutrient solution with normal Fe concentration forcultivation, and half were transplanted into a nutrient solution with low Fefor cultivation, and samples were taken 10 days later. The heat map shows thegene expression level indicated by the TPM value. Differentially expressedgenes that show higher expression levels under the control (normal Fe: 50 μM)and treatment (low Fe: 2 μM) are indicated by asterisks. Figure S5. Differential expression profile ofphotosynthesis-related genes in wheat plants under low Fe stress. Differentialexpression profile of genes related to photosynthesis-antenna proteins (A) andcarotenoid biosynthesis (B). The expression pattern of genes involved in thetransformation of chlorophyll a and chlorophyll b (C) and the synthesis ofcarotenoids under low Fe stress (D). The expression pattern of chlorophyll a/bbinding proteins (E), Photosynthesis II reaction proteins (F), RuBisCo subunitbinding proteins (G), Mg2+ chelatase (H), Ribulose bisphosphatecarboxylase/oxygenase activase (I). For transcriptome sequencing, selecteduniform wheat plants after germination, half of which were transplanted into anutrient solution with normal Fe concentration for cultivation, and half weretransplanted into a nutrient solution with low Fe for cultivation, and sampleswere taken 10 days later. The heat map shows the gene expression levelindicated by the TPM value. Differentially expressed genes that show higherexpression levels under he control (normal Fe: 50 μM) and treatment (low Fe: 2μM) are indicated by asterisks. FigureS6. Heat map of expression of genes involved incell cycle and ROS metabolism. (A) Graph of expression abundance of cellcycle-related genes. Differential expression profile of ROS synthesis (B) andclearance related genes (C). For transcriptome sequencing, selected uniformwheat plants after germination, half of which were transplanted into a nutrientsolution with normal Fe concentration for cultivation, and half weretransplanted into a nutrient solution with low Fe for cultivation, and sampleswere taken 10 days later. The heat map shows the gene expression levelindicated by the TPM value. Differentially expressed genes that show higher expressionlevels under he control (normal Fe: 50 μM) and treatment (low Fe: 2 μM) areindicated by asterisks. Figure S7. Co-expressionnetwork analysis of Fe absorption and transport related genes under low Festress conditions. Cycle nodes represent genes, and the size of the nodesrepresents the power of the interrelation among the nodes by degree value.Edges between two nodes represent interactions between genes. Figure S8. Natural variation in wheatresistance to low Fe stress and morphological identification of resistant andsensitive genotypes. (A) Variation in low Fe stress in a panel comprising 386wheat accessions. The root length and chlorophyll value of wheat were used forpopulation screening. After germination, uniform seedlings were selected andgrown for 10 d in Hoagland nutrient solution. Then Half of the seedlings werecultivated in a nutrient solution containing sufficient Fe, and half werecultivated in a nutrient solution containing low Fe stress. Min, minimum; max,maximum; CV, coefficient of variation. (B) Growth performance of ‘zhengmai1860’and ‘zhoumai32’ under low Fe stress. For the low Fe treatment, the wheat plantswere grown hydroponically under high Fe (50 μM) and low Fe (2 μM) conditionsfor 10 d, respectively. Bar: 5cm. (C) Relative expression of TaIRT1b-4A,TaNAS2-6D, TaNAS1a-6A, TaNAS1a-6B, and TaNAAT1b-1D in the shoots and roots ofthe two genotypes grown under normal Fe and low Fe conditions. For the low Fetreatment, the wheat plants were grown hydroponically under high Fe (50 μM) andlow Fe (2 μM) conditions for 10 d, respectively. Data are means (±SD), n=3. Thesignificant difference was determined using Student’s t-test: **P

Additional file 2: Figure S2. Identification and characterization of conserved domains in BBXs in NCBI database. Figure S2–1 Identification and characterization of the conserved domains in BnaBBXs. Figure S2–2 Identification and characterization of the conserved domains in BoBBXs. Figure S2–3 Identification and characterization of the conserved domains in BrBBXs. Figure S2–4 Identification and characterization of the conserved domains in CrBBXs. Figure S2–5 Identification and characterization of the conserved domains in CsBBXs. Figure S2–6 Identification and characterization of the conserved domains in BnBBXs. Figure S2–7 Identification and characterization of the conserved domains in BjBBXs. Figure S2–8 Identification and characterization of the conserved domains in BcBBXs.

Additional file 1: Figure S1. Chromosomal location of BBX genes on Brassicaceae chromosomes. Figure S1–1 The location of BBX genes on B. napus chromosomes. Figure S1–2 The location of BBX genes on B. oleracea chromosomes. Figure S1–3 The location of BBX genes on B. rapa chromosomes and scaffold. Figure S1–4 The location of BBX genes on C. rubella scaffolds. Figure S1–5 The location of BBX genes on C. sativa chromosomes and scaffold. Figure S1–6 Chromosomal location of B. nigra BBX genes. Figure S1–7 Chromosomal location of B.juncea BBX genes. Figure S1–8 Chromosomal location of B. carinata BBX genes.

Additional file 7: Figure S7. Promoter analysis of BBX genes. Figure S7–1 Cis-element identified in promoters of BnaBBXs. Figure S7–2 Cis-element identified in promoters of BoBBXs. Figure S7–3 Cis-element identified in promoters of BrBBXs. Figure S7–4 Cis-element identified in promoters of CrBBXs. Figure S7–5 Cis-element identified in promoters of CsBBXs. Figure S7–7 Cis-element identified in promoters of BjBBXs. Figure S7–8 Cis-element identified in promoters of BcBBXs.

Additional file 6: Figure S6. Synteny of BBX genes between A. thaliana and each other Brassicaceae. Figure S6–1 Synteny of BBX genes between A. thaliana and B. napus. Figure S6–2 Synteny of BBX genes between A. thaliana and B. oleracea. Figure S6–3 Synteny of BBX genes between A. thaliana and B. rapa. Figure S6–4 Synteny of BBX genes between A. thaliana and C. rubella. Figure S6–5 Synteny of BBX genes between A. thaliana and C. sativa. Figure S6–6 Synteny of BBX genes between A. thaliana and B. nigra. Figure S6–7 Synteny of BBX genes between A. thaliana and B.juncea. S109, C668, C1065 and C306 respectively indicates Super_scaffold_109_3169392_4351964_52914_567609, Contig668_1_483987, Contig1065 and Contig306 in the B.juncea genome. Figure S6–8 Synteny of BBX genes between A. thaliana and B. carinata. J0021.1, J1585.1, J1599.1 and J1604.1 respectively indicates JAAMPC010000021.1, J AAMPC010001585.1, J AAMPC010001599.1 and J AAMPC010001604.1 in B. carinata genome.

Additional file 4: Figure S4. Structure analysis of BBX genes. Figure S4–1 Exon–intron structures of BnaBBX genes. Figure S4–2 Exon–intron structures of BoBBX genes. Figure S4–3 Exon–intron structures of BrBBX genes. Figure S4–4 Exon–intron structures of CrBBX genes. Figure S4–5 Exon–intron structures of CsBBX genes. Figure S4–6 Exon–intron structures of BnBBX genes. Figure S4–7 Exon–intron structures of BjBBX genes. Figure S4–8 Exon–intron structures of BcBBX genes.

Additional file 3: Figure S3. Identification and characterization of conserved domains in BBXs in pfam database. Figure S3–1 Identification and characterization of the conserved domains in BnaBBXs. Figure S3–2 Identification and characterization of the conserved domains in BoBBXs. Figure S3–3 Identification and characterization of the conserved domains in BrBBXs. Figure S3–4 Identification and characterization of the conserved domains in CrBBXs. Figure S3–5 Identification and characterization of the conserved domains in CsBBXs. Figure S3–6 Identification and characterization of the conserved domains in BnBBXs. Figure S3–7 Identification and characterization of the conserved domains in BjBBXs. Figure S3–8 Identification and characterization of the conserved domains in BcBBXs.

Additional file 1.