74 results on '"Yang Soo Moon"'
Search Results
2. Intestinal microbial composition changes induced by Lactobacillus plantarum GBL 16, 17 fermented feed and intestinal immune homeostasis regulation in pigs
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Da Yoon Yu, Sang-Hyon Oh, In Sung Kim, Gwang Il Kim, Jeong A Kim, Yang Soo Moon, Jae Cheol Jang, Sang Suk Lee, Jong Hyun Jung, Jun Park, and Kwang Keun Cho
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Rubus coreanus byproducts ,Probiotics ,Pig ,Intestinal microbiota ,Immune homeostasis ,Animal culture ,SF1-1100 - Abstract
In this study, Rubus coreanus (R. coreanus) byproducts with high polyphenol content were fermented with R. coreanus-derived lactic acid bacteria (Lactobacillus plantarum GBL 16 and 17). Then the effect of R. coreanus-derived lactic acid bacteria fermented feed (RC-LAB fermented feed) with probiotics (Bacillus subtills, Aspergillus oryzae, Yeast) as a feed additive for pigs on the composition of intestinal microbes and the regulation of intestinal immune homeostasis was investigated. Seventy-two finishing Berkshire pigs were randomly allotted to four different treatment groups and 18 replicates. RC-LAB fermented feed with probiotics increased the genera Lactobacillus, Streptococcus, Mitsuokella, Prevotella, Bacteroides spp., Roseburia spp., and Faecalibacterium prausnitzii, which are beneficial bacteria of the digestive tract of pigs. Also, RC-LAB fermented feed with probiotics decreased the genera Clostridium, Terrisporobacter, Romboutsia, Kandleria, Megasphaera and Escherichia, which are harmful bacteria. In particular, the relative abundance of the genera Lactobacillus and Streptococcus increased by an average of 8.51% and 4.68% in the treatment groups and the classes Clostridia and genera Escherichia decreased by an average of 27.05% and 2.85% in the treatment groups. In mesenteric lymph nodes (MLN) and spleens, the mRNA expression of transcription factors and cytokines in Th1 and Treg cells increased and the mRNA expression of Th2 and Th17 transcription factors and cytokines decreased, indicating a regulatory effect on intestinal immune homeostasis. RC-LAB fermented feed regulates gut immune homeostasis by influencing the composition of beneficial and detrimental microorganisms in the gut and regulating the balance of Th1/Th2 and Th17/Treg cells.
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- 2022
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3. Effects of byproducts on intestinal microbiota and the immune modulation
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Da Yoon Yu, Sung Hak Kim, Jeong A Kim, In Sung Kim, Yang Soo Moon, Sang Suk Lee, Hwa Chun Park, Jong Hyun Jung, Yi Hyung Chung, Dae Keun Shin, Ki Chang Nam, In Soon Choi, and Kwang Keun Cho
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Th1/Th2 Balance ,Galectin-9 ,Illumina Sequencing ,Intestinal Microbiota ,Immune Modulation ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
Objective Although the efficacy of Rubus coreanus (RC) byproducts as a feed additive has been recognized, its effects on intestinal microorganisms and the immune system are still unknown. Methods Six-week-old male rats were treated with 0.5% RC (T1), 1.0% RC (T2), and 1.5% RC (T3) for 4 weeks. Results We found that treatment with RC byproducts significantly increased the daily gain of body weight and feed intake. Treg-cell differentiation was enhanced in the mesenteric lymph nodes and spleen from the rats fed with RC byproducts. Illumina sequencing showed that bacteria in the phylum Firmicutes decreased and while those in the phylum Bacteroidetes increased in RC-treated groups. Particularly, the pathogenic microorganisms in the family Peptococcaceae decreased, and the non-pathogenic families Lachnospiraceae and S24-7 increased. Quantitative polymerase chain reaction analysis showed that the RC byproducts increased the lactic acid bacteria Bifidobacterium spp., Oscillospira spp., Leuconostoc citreum, and Weissella cibaria in a concentration-dependent manner. Conclusion RC byproducts may be effective in immunomodulation by affecting intestinal microorganisms.
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- 2018
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4. Suppression of fatty acid synthase promoter by polyunsaturated fatty acids
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Yang Soo Moon, Maria-Jesus Latasa, Michael J. Griffin, and Hei Sook Sul
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SREBP ,transgenic mice ,−150 SRE ,−65 E-box ,HepG2 ,Biochemistry ,QD415-436 - Abstract
Dietary polyunsaturated fat is known to suppress expression of fatty acid synthase (FAS), a central enzyme in de novo lipogenesis. The sterol regulatory element-binding protein (SREBP) has recently been shown to be involved in this suppression. We previously reported that the first 2.1 kb of the FAS promoter are sufficient for transcriptional induction by a high carbohydrate diet as well as suppression by polyunsaturated fat in transgenic mice. Here, we first examined the DNA sequences responsible for SREBP-mediated suppression of FAS promoter activity by polyunsaturated fatty acids (PUFA) in vivo. Feeding polyunsaturated fat prevented both the low-level activation of the −278 FAS promoter which contains the −150 sterol response element (SRE), as well as the maximal activation of the longer −444 FAS promoter. We observed that ectopic expression of the activated form of SREBP in liver prevented PUFA-mediated suppression of both the endogenous FAS and FAS promoter-reporter transgene expression. We also found that the promoter region required for PUFA suppression in vivo is located between −278 to −131, where SREBP functions. Using HepG2 cells, we further examined the specific FAS promoter elements required for PUFA suppression. We found that the −150 SRE, as well as the –65 E-Box, contribute to PUFA suppression of the FAS promoter, at least in vitro.—Moon, Y. S., M-J. Latasa, M. J. Griffin, and H. S. Sul. Suppression of fatty acid synthase promoter by polyunsaturated fatty acids. J. Lipid Res. 2002. 43: 691–698.
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- 2002
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5. The Link between Birds and Dinosaurs: Aves Evolved from Dinosaurs
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Yang Soo Moon
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- 2022
6. Effects of Coenzyme Q10 on the Antioxidant System in Blood and Liver of Laying Hens
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Yang-Soo Moon and In-Surk Jang
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- 2022
7. Effects of lactic acid bacteria (LAB) fermented feed and three types of LAB (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats
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Da Yoon Yu, Sang-Hyon Oh, In Sung Kim, Gwang Il Kim, Jeong A Kim, Yang Soo Moon, Jae Cheol Jang, Sang Suk Lee, Jong Hyun Jung, Hwa Chun Park, and Kwang Keun Cho
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General Veterinary ,Physiology ,Genetics ,Animal Science and Zoology ,Food Science - Abstract
Objective: In this study, we investigated the effects of Rubus coreanus-derived lactic acid bacteria (LAB) fermented feed (RC-LAB fermented feed) and three types of LAB (Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis) on the expression of transcription factors and cytokines in Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens of rats. In addition, the effect on intestinal microbiota composition and body weight was investigated.Methods: Five-week-old male rats were assigned to five treatments and eight replicates. The expression of transcription factors and cytokines of Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens was analyzed using real-time reverse transcriptase polymerase chain reaction assays. Intestinal tract microbiota compositions were analyzed by next-generation sequencing and quantitative polymerase chain reaction assays.Results: RC-LAB fermented feed and three types of LAB increased the expression of transcription factors and cytokines in Th1, Treg cells and Galectin-9, but decreased in Th2 and Th17 cells. In addition, the intestinal microbiota composition changed, the body weight and Firmicutes to Bacteroidetes (F/B) ratio decreased, and the relative abundance of LAB increased.Conclusion: LAB fermented feed and three types of LAB showed an immune modulation effect by inducing T cell polarization and increased LAB in the intestinal microbiota.
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- 2022
8. Heat Shock Proteins in Heat Stressed Chickens
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Yang Soo Moon
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Animal Welfare (journal) ,Chemistry ,Heat shock protein ,Cell biology ,Heat stress - Published
- 2020
9. Comparison of Physicochemical Pork Quality of Berkshires Supplemented with Rubus coreanus By-product
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Yang Soo Moon, Nam Ki Chang, Hwa Chun Park, cho kwang-keun, Ji-Young Park, Jong Hyun Jung, and Chung Yi Hyung
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biology ,media_common.quotation_subject ,By-product ,Rubus coreanus ,Quality (business) ,Food science ,biology.organism_classification ,media_common - Published
- 2020
10. Oral Administration of ¥ Glucan and Lactobacillus plantarum Alleviates Atopic Dermatitis-Like Symptoms
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Gwang Il Kim, Sung Hak Kim, Kwang Keun Cho, Jong Min Lim, In Soon Choi, Sang-Suk Lee, Jeong A Kim, Yang Soo Moon, Seung Ho Lee, Da Yoon Yu, Bishnu Adhikari, Young Min Kwon, and In Sung Kim
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biology ,business.industry ,Lachnospiraceae ,General Medicine ,Atopic dermatitis ,Gut flora ,Pharmacology ,biology.organism_classification ,medicine.disease ,Applied Microbiology and Biotechnology ,chemistry.chemical_compound ,Immune system ,chemistry ,Oral administration ,Medicine ,Roseburia ,business ,Lactobacillus plantarum ,Histamine ,Biotechnology - Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease of mainly infants and children. Currently, the development of safe and effective treatments for AD is urgently required. The present study was conducted to investigate the immunomodulatory effects of yeast-extracted β-1,3/1,6-glucan and/or Lactobacillus plantarum (L. plantarum) LM1004 against AD-like symptoms. To purpose, β-1,3/1,6-glucan and/or L. plantarum LM1004 were orally administered to AD-induced animal models of rat (histamine-induced vasodilation) and mouse (pruritus and contact dermatitis) exhibiting different symptoms of AD. We then investigated the treatment effects on AD-like symptoms, gene expression of immune-related factors, and gut microbiomes. Oral administration of β-1,3/1,6-glucan (0.01 g/kg initial body weight) and/or 2 × 1012 cells/g L. plantarum LM1004 (0.01 g/kg initial body weight) to ADinduced animal models showed significantly reduced vasodilation in the rat model, and pruritus, edema, and serum histamine in the mouse models (p < 0.05). Interestingly, β-1,3/1,6- glucan and/or L. plantarum LM1004 significantly decreased the mRNA levels of Th2 and Th17 cell transcription factors, while the transcription factors of Th1 and Treg cells, galactin-9, filaggrin increased, which are indicative of enhanced immunomodulation (p < 0.05). Moreover, in rats with no AD induction, the same treatments significantly increased the relative abundance of phylum Bacteroidetes and the genus Bacteroides. Furthermore, bacterial taxa associated with butyrate production such as, Lachnospiraceae and Ruminococcaceae at family, and Roseburia at genus level were increased in the treated groups. These findings suggest that the dietary supplementation of β-1,3/1,6-glucan and/or L. plantarum LM1004 has a great potential for treatment of AD as well as obesity in humans through mechanisms that might involve modulation of host immune systems and gut microbiota.
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- 2019
11. Thermal Stress and Muscle Development in Early Posthatch Broilers
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Yang, Soo Moon, primary
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- 2021
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12. Perinatal Nutrition, Post-Hatch Holding Time and In ovo feeding
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Yang Soo Moon
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Animal science ,business.industry ,Medicine ,In ovo ,business ,Perinatal nutrition ,Holding time - Published
- 2019
13. Galectin-9 Induced by Dietary Prebiotics Regulates Immunomodulation to Reduce Atopic Dermatitis Symptoms in 1-Chloro-2,4-Dinitrobenzene (DNCB)-Treated NC/Nga Mice
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In Soon Choi, Kwang Keun Cho, Jeong A Kim, Sung Chan Kim, Gwang Il Kim, Cheol-Heui Yun, Sung Hak Kim, Yang Soo Moon, Seung Ho Lee, In Sung Kim, Sang-Suk Lee, and Da Yoon Yu
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Male ,Dinitrobenzene ,medicine.medical_treatment ,Galectins ,T-Lymphocytes ,Inulin ,Pharmacology ,Gut flora ,Applied Microbiology and Biotechnology ,Dermatitis, Atopic ,Immunomodulation ,chemistry.chemical_compound ,Mice ,In vivo ,medicine ,Dinitrochlorobenzene ,Animals ,Humans ,Mesentery ,Galectin ,Skin ,biology ,Bacteria ,Prebiotic ,General Medicine ,Atopic dermatitis ,medicine.disease ,biology.organism_classification ,In vitro ,Gastrointestinal Microbiome ,Disease Models, Animal ,Prebiotics ,chemistry ,Toll-Like Receptor 9 ,Dietary Supplements ,Lymph Nodes ,HT29 Cells ,Biotechnology - Abstract
Atopic dermatitis (AD) is a skin disorder that causes chronic itch. We investigated the inhibitory effects of a mixture of prebiotic short-chain galacto-oligosaccharides and long-chain fructooligosaccharides (scGOS/lcFOS), inulin, or β-glucan on AD development in 1-chloro-2,4- dinitrobenzene (DNCB)-treated NC/Nga mice. Mice were randomly assigned to six groups: untreated mice, AD control, positive control (DNCB-treated NC/Nga mice fed a dietary supplement of Zyrtec), and DNCB-treated NC/Nga mice fed a dietary supplement of prebiotics such as scGOS/lcFOS (T1), inulin (T2), or β-glucan (T3). The prebiotic treatment groups (T1, T2, and T3) showed suppression of AD symptoms, Th2 cell differentiation, and AD-like skin lesions induced by DNCB. In addition, prebiotic treatment also reduced the number of microorganisms such as Firmicutes, which is associated with AD symptoms, and increased the levels of Bacteroidetes and Ruminococcaceae, which are associated with alleviation of AD symptoms. Our findings demonstrate the inhibitory effects of prebiotics on AD development by improving the Th1/Th2 cytokine balance and beneficial symbiotic microorganisms in in vitro and in vivo models.
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- 2020
14. Lipid Metabolism and Fatty Liver in Poultry
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Yang Soo Moon
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0301 basic medicine ,03 medical and health sciences ,030104 developmental biology ,Biochemistry ,Chemistry ,Fatty liver ,0402 animal and dairy science ,medicine ,Lipid metabolism ,04 agricultural and veterinary sciences ,medicine.disease ,040201 dairy & animal science - Published
- 2018
15. Effects of Lipopolysaccride-induced Stressor on the Expression of Stress-related Genes in Two Breeds of Chickens
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Sohn Sea Hwan, Yang Soo Moon, and InSurk Jang
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Stress related genes ,Genetics ,Expression (architecture) ,Microarray ,Gene expression ,Stressor ,Biology - Published
- 2017
16. Oral Administration of β-Glucan and
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In Sung, Kim, Seung Ho, Lee, Young Min, Kwon, Bishnu, Adhikari, Jeong A, Kim, Da Yoon, Yu, Gwang Il, Kim, Jong Min, Lim, Sung Hak, Kim, Sang Suk, Lee, Yang Soo, Moon, In Soon, Choi, and Kwang Keun, Cho
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Male ,beta-Glucans ,Probiotics ,T-Lymphocytes ,Administration, Oral ,Filaggrin Proteins ,Dermatitis, Atopic ,Gastrointestinal Microbiome ,Rats, Sprague-Dawley ,Disease Models, Animal ,Mice ,Treatment Outcome ,Animals ,Cytokines ,Immunologic Factors ,Lactobacillus plantarum ,Skin ,Transcription Factors - Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease of mainly infants and children. Currently, the development of safe and effective treatments for AD is urgently required. The present study was conducted to investigate the immunomodulatory effects of yeast-extracted β-1,3/1,6-glucan and/or
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- 2019
17. Effects of Stocking Density and Lipopolysaccharide on Immune Organ Weights, Blood Biochemical Profiles and the mRNA Expression of Pro-inflammatory Cytokines in Chicks
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Sea Hwan Sohn, Ha-Na Kim, In-Surk Jang, Yang Soo Moon, and Min-Hye Song
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medicine.medical_specialty ,Lipopolysaccharide ,biology ,Interleukin ,Proinflammatory cytokine ,chemistry.chemical_compound ,Immune system ,Endocrinology ,Blood chemistry ,chemistry ,Internal medicine ,Blood plasma ,Gene expression ,biology.protein ,medicine ,Interleukin 6 - Published
- 2016
18. Effects of Coenzyme Q10 on the Expression of Genes involved in Lipid Metabolism in Laying Hens
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In Surk Jang and Yang Soo Moon
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Coenzyme Q10 ,medicine.medical_specialty ,Cholesterol ,Lipid metabolism ,Reductase ,Biology ,Sterol ,Sterol regulatory element-binding protein ,chemistry.chemical_compound ,Basal (phylogenetics) ,Endocrinology ,chemistry ,Internal medicine ,medicine ,lipids (amino acids, peptides, and proteins) ,Liver X receptor - Abstract
Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju 52725, Korea ABSTRACT The aim of this study was to investigate the expression patterns of key genes involved in lipid metabolism in response to dietary Coenzyme Q10 (CoQ10) in hens. A total of 36 forty week-old Lohmann Brown were randomly allocated into 3 groups consisting of 4 replicates of 3 birds. Laying hens were subjected to one of following treatments: Control (BD, basal diet), T1 (BD+ CoQ10 100 mg/kg diet) and T2 (BD+ micellar of CoQ10 100 mg/kg diet). Birds were fed ad libitum a basal diet or the basal diet supplemented with CoQ10 for 5 weeks. Total RNA was extracted from the liver for quantitative RT-PCR. The mRNA levels of HMG-CoA reductase(HMGCR) and sterol regulatory element-binding proteins(SREBP)2 were decreased more than 3050% in the liver of birds fed a basal diet supplemented with CoQ10 (p
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- 2016
19. Effect of Supplementation of Acanthopanax senticosus on Growth Performance, Blood Biochemical Profiles and Expression of Pro-Inflammatory Cytokines in Broiler Chicks
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Sea Hwan Sohn, Yang Soo Moon, and In-Surk Jang
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medicine.medical_specialty ,Triglyceride ,Feed additive ,Broiler ,Albumin ,Biology ,Feed conversion ratio ,Proinflammatory cytokine ,chemistry.chemical_compound ,Basal (phylogenetics) ,Immune system ,Endocrinology ,chemistry ,Internal medicine ,medicine - Abstract
Dept. of Animal Science and Biotechnology, the Regional Animal Research Center (RAIC),Gyeongnam National University of Science and Technology, Jinju 52725, Korea,ABSTRACT This study was performed to examine the effects of dietary Acanthopanax senticosus (AS) on growth performance, immune organ weights, blood biochemical parameters and the expression of pro-inflammatory cytokines in broiler chicks. A total of 120 4-day-old birds were given a basal diet (CON) or a basal diet supplemented with 0.5% (AS1) or 1.0% (AS2) AS powder until the birds were 35 days of age. There was no difference in body weight, total gain, feed intake or immune organ weights among the treatment groups. However, the feed conversion ratio in the AS2 group was lower (p
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- 2015
20. Effects of Lycopene on the Expression of Lipid Metabolism, Glucose Transport and Pro-Inflammatory Related Genes in Chickens
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In Surk Jang and Yang Soo Moon
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medicine.medical_specialty ,Glucose transporter ,Lipid metabolism ,Metabolism ,Biology ,Lycopene ,Fatty acid-binding protein ,chemistry.chemical_compound ,Endocrinology ,Carnitine palmitoyltransferase 1 ,chemistry ,Biochemistry ,Internal medicine ,Gene expression ,medicine ,Carnitine ,medicine.drug - Abstract
Dept. of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju 52725, KoreaABSTRACT The present study aimed to investigate the effects of lycopene on hepatic metabolic- and immune-related gene expression in laying hens. A total of 48 25-week-old White Leghorn hens were randomly allocated into four groups consisting of four replicates of three birds: control (basal diet), T1 (basal diet + 10 mg/kg of tomato powder-containing lycopene), T2 (basal diet + 10 mg/kg of micelles of tomato powder-containing lycopene), and T3 (basal diet + 10 mg/kg of purified lycopene). Chickens were fed ad libitum for 5 weeks, and then total RNA was extracted from the livers for quantitative RT-PCR analysis. Peroxisome proliferator-activated receptor ɣ (PPARɣ) expression was decreased in the liver of chickens after lycopene supplementation (P
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- 2015
21. Overexpression of Pref-1 in pancreatic islet β-cells in mice causes hyperinsulinemia with increased islet mass and insulin secretion
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Yuhui Wang, Karim Roder, Kichoon Lee, Hei Sook Sul, Chulho Kang, Yang Soo Moon, Kee-Hong Kim, and Maryam Ahmadian
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endocrine system diseases ,medicine.medical_treatment ,Islet beta-cells ,Medical Biochemistry and Metabolomics ,Inbred C57BL ,Biochemistry ,Transgenic ,Hyperinsulinemia ,Mice ,Pancreatic tumor ,Insulin-Secreting Cells ,Insulin Secretion ,Transgenic mice ,2.1 Biological and endogenous factors ,Insulin ,Aetiology ,geography.geographical_feature_category ,Chemistry ,Diabetes ,Pref-1/Dlk1 ,Islet ,Up-Regulation ,Insulin oscillation ,medicine.anatomical_structure ,Intercellular Signaling Peptides and Proteins ,Female ,Pancreas ,Genetically modified mouse ,Biochemistry & Molecular Biology ,endocrine system ,medicine.medical_specialty ,Islet β-cells ,Biophysics ,Mice, Transgenic ,Autoimmune Disease ,Article ,Medicinal and Biomolecular Chemistry ,Hyperinsulinism ,Internal medicine ,medicine ,Animals ,Molecular Biology ,Metabolic and endocrine ,Cell Proliferation ,geography ,Pancreatic islets ,Calcium-Binding Proteins ,Cell Biology ,medicine.disease ,Mice, Inbred C57BL ,Endocrinology ,Biochemistry and Cell Biology ,Insulin Resistance ,Digestive Diseases - Abstract
Preadipocyte factor-1 (Pref-1) is made as a transmembrane protein containing EGF-repeats at the extracellular domain that can be cleaved to generate a biologically active soluble form. Pref-1 is found in islet β-cells and its level has been reported to increase in neonatal rat islets upon growth hormone treatment. We found here that Pref-1 can promote growth of pancreatic tumor derived AR42J cells. To examine Pref-1 function in pancreatic islets invivo, we generated transgenic mouse lines overexpressing the Pref-1/hFc in islet β-cells using rat insulin II promoter (RIP). These transgenic mice exhibit an increase in islet mass with higher proportion of larger islets in pancreas compared to wild-type littermates. This is in contrast to pancreas from Pref-1 null mice that show higher proportion of smaller islets. Insulin expression and insulin secretion from pancreatic islets from RIP-Pref-1/hFc transgenic mice are increased also. Thus, RIP-Pref-1/hFc transgenic mice show normal glucose levels but with higher plasma insulin levels in both fasting and fed conditions. These mice show improved glucose tolerance. Taken together, we conclude Pref-1 as a positive regulator of islet β-cells and insulin production.
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- 2015
22. Effects of High Stocking Density on the Expression of Metabolic Related Genes in Two Strains of Chickens
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Sea Hwan Sohn, In Surk Jang, Yang Soo Moon, and Young Sook An
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biology ,business.industry ,Lipid metabolism ,Carbohydrate metabolism ,Marker gene ,Biotechnology ,Hsp70 ,Korean Native ,Animal science ,Stocking ,biology.protein ,Unfolded protein response ,GLUT2 ,business - Abstract
Chickens are exposed to the external and internal stressors such as low and high temperature, high stocking density, feed restriction and disease. There have been a few studies on gene expressions through the investigation of chickens under direct exposure to the stress of high stocking density. The objective of the present study was to determine the expressions of genes associated with stress, endoplasmic reticulum (ER)-stress, lipid and glucose metabolism in two strains of chickens, Korean Native Chicken (KNC) and White Leghorn (WL), raised in high stocking density. A total of 164 chickens aged 40 weeks were randomly allotted to a 540 cm 2 /bird stocking density (control), whereas the chickens in a high density group were assigned in a 311 cm 2 /bird stocking density with feeding ad libitum for 10 weeks. Total RNA was extracted from the live for qRT-PCR. The expression levels of hsp70 and hsp90α were higher in WL subjected to stress with high stocking density compared with those genes in control (P
- Published
- 2015
23. Effects of Selenate on Adipocyte Differentiation and the Expression of Selenoproteins in 3T3-L1 Cells
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Yang Soo Moon and Seol Hui Park
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chemistry.chemical_classification ,medicine.medical_specialty ,SEPP1 ,3T3-L1 Cells ,Biology ,Molecular biology ,Selenate ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Adipogenesis ,Adipocyte ,Internal medicine ,Gene expression ,medicine ,Selenoprotein ,Intracellular - Abstract
Department of Animal Science & Biotechnology, Gyeongnam Nationa l University of Science and Technology, Jinju 660-758, KoreaReceived September 11, 2014/Revised October 8, 2014/Accepted October 14, 2014The purpose of this study was to determine the effect of selenate on adipocyte differentiation and to identify genes involved in the modulation of adipogenesis in 3T3-L1 cells. To test the effect of selenate on adipocyte differentiation, adipogenesis was induced in cells using various concentrations (0–00 μM) of selenate. Various phases of adipogenesis were induced: postconfluent (PC), early phase (EP, d0-d2), postmitotic growth arrest (PM, d2-d4), and all period (AP). The PC cells exposed to selenate for 24 h displayed dose-dependent inhibition of intracellular lipid droplet accumulation on day 6 of adipogenesis. Two days of selenate treatment at EP or AP inhibited adipogenesis, with an approx-imately 20–80% reduction in lipid accumulation compared to that of a control (p
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- 2014
24. The Effect of Stocking Density and Strain on the Performance and Physiological Adaptive Responses in Broiler Chickens
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Sea Hwan Sohn, Seo-Hyun Yun, Min-Hye Song, Jong-Sun Kim, In-Surk Jang, Yang-Soo Moon, Young-Hyun Ko, and Se-Yun Kim
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chemistry.chemical_classification ,Antioxidant ,biology ,Strain (chemistry) ,Glutathione peroxidase ,medicine.medical_treatment ,Broiler ,Anatomy ,Proinflammatory cytokine ,Superoxide dismutase ,Animal science ,Stocking ,chemistry ,Catalase ,biology.protein ,medicine - Abstract
The aim of this study was to determine the effects of stocking density and strain on the performance and physiological adaptive responses including the plasma corticosterone content and the level of mRNA expression of pro- inflammatory cytokines and antioxidant enzymes in broiler chicks. A total of 300 birds of two strains (150 Ross strain vs. 150 Cobb strain) aged 3-d old were allotted into two stocking densities (standard stocking density, 0.046 m 2 /bird vs. high stocking density, 0.023 m 2 /bird) in battery cages by 2×2 factorial designs with ten replicates until 35 d of age. There was no significant strain effect on body weight, feed intakes and feed to gain ratio and the relative organ weights. However body weight, feed intakes and relative organ weight were found to be significantly (P
- Published
- 2014
25. Comparison of Stress Response between Korean Native Chickens and Single Comb White Leghorns subjected to a High Stocking Density
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Dhan Bee Park, Eun Jung Cho, Yang Soo Moon, In Surk Jang, and Sea Hwan Sohn
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Genetics ,animal structures ,DNA damage ,In situ hybridization ,Biology ,Hsp70 ,law.invention ,Comet assay ,Andrology ,Korean Native ,Stocking ,law ,Heat shock protein ,Polymerase chain reaction - Abstract
Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju 660-758, KoreaABSTRACT With Single Comb White Leghorn (WL) and Korean Native Chicken (KNC) breeds, we compared the stress response with chicken breeds that were subjected to a high stocking density. Stress response was analyzed by the quantity of telomeric DNA, the rate of DNA damage and the expression levels of heat shock proteins (HSPs) and hydroxyl-3- methyl-glutaryl coenzyme A reductase (HMGCR) genes on tissues and blood. The telomere length and telomere shortening rates were analyzed by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes and tissues. The DNA damage rate of lymphocytes was quantified by the comet assay. The expression levels of HSP70, HSP90-α, HSP90-β and HMGCR genes were measured by quantitative real-time polymerase chain reaction in lymphocytes. There was no significant difference between KNC and WL in body weight, weight gain, telomere shortening rate and DNA damage rate. However, the growth rate significantly decreased in chickens raised under high stocking density conditions, as compared to the control group. The telomere-shortening rate, DNA damage and HSPs expression of the lymphocytes were significantly higher in the high stocking density group than the control. The stress condition and breeds had a significant effect on the expressions of HSP70, HSP90-α and HSP90-β in lymphocytes, except HMGCR. The stress response of WL was higher than that of KNC, as analyzed to the expression of HSP70 and HSP90-α. Therefore, we concluded that the chickens which were exposed to a high stocking density had increased the individual physiological stress response regardless of breeds, and White Leghorns are more susceptible to stress condition than Korean Native Chickens.(Key words : stress response, telomere, DNA damage, HSP, Korean Native Chicken, Leghorn)
- Published
- 2014
26. Effects of Vitamin C or E on the Pro-inflammatory Cytokines, Heat Shock Protein 70 and Antioxidant Status in Broiler Chicks under Summer Conditions
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Sea Hwan Sohn, Young-Hyun Ko, Yang Soo Moon, and In-Surk Jang
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medicine.medical_specialty ,Antioxidant ,Inflammatory Cytokines ,medicine.medical_treatment ,Summer ,lcsh:Animal biochemistry ,Biology ,Article ,Proinflammatory cytokine ,Lipid peroxidation ,chemistry.chemical_compound ,Basal (phylogenetics) ,Internal medicine ,medicine ,Vitamin E ,Vitamin C ,lcsh:QP501-801 ,lcsh:SF1-1100 ,Broiler ,Hsp70 ,Endocrinology ,chemistry ,Animal Science and Zoology ,lcsh:Animal culture ,Food Science - Abstract
The present study was carried out to investigate the effects of dietary antioxidants on pro-inflammatory cytokines, heat shock protein (HSP) and antioxidant status in broiler chicks under summer conditions. A total of 162, 3-d-old broiler chicks were randomly assigned to a basal diet (CON) and the basal diet supplemented with vitamin C (200 mg/kg diet, VCD) or vitamin E (100 mg/kg, VED) until 35 day of age. All birds were exposed to summer diurnal heat stress at average daily fluctuations of temperature between 32 degrees C to 34 degrees C at day to 27 degrees C to 29 degrees C at night for the entire feeding periods. There was no significant difference in body weight, feed to gain ratio and the relative organ weight except the thymus in response to dietary vitamin C or E supplementation. However, the mRNA expression of interleukin (IL)-1 beta, IL-6, interferon (IFN)-gamma, Toll like receptor (TLR)-4 and HSP70 in the liver of birds fed diet containing vitamin C significantly (p
- Published
- 2014
27. The Effects of Dietary Supplementation of Vitamin C or E on the Expressions of Endoplasmic Reticulum Stress, Lipid and Glucose Metabolism Associated Genes in Broiler Chickens
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Yang Soo Moon, Sea Hwan Sohn, Jeong Geun Park, In Surk Jang, and Young Sook An
- Subjects
medicine.medical_specialty ,Vitamin C ,Endoplasmic reticulum ,Vitamin E ,medicine.medical_treatment ,Broiler ,Biology ,Carbohydrate metabolism ,Ascorbic acid ,Hsp70 ,Endocrinology ,Internal medicine ,medicine ,Tocopherol - Abstract
This study was conducted to evaluate the effects of dietary supplementation of vitamin C or E on the expressions of endoplasmic reticulum (ER) stress, lipid and glucose metabolism associated genes in broiler chickens. A total of 216 one-day-old male broilers was randomly alloted to 4 treatments with 6 replicate pens per treatment and 9 broilers per pen for 35 days. The dietary treatments were control, vitamin C (control diet + ascorbic acid 200 mg/kg diet), vitamin E (control diet + -tocopherol 100 mg/kg diet), vitamin C + E (control diet + vitamin C 200 mg/kg + vitamin E 100 mg/kg), respectively. To evaluate gene expressions by quantitative real-time polymerase chain reaction, total RNA was extracted from the liver of the chicken at 35 days of age. Dietary supplementation of vitamins was significantly down-regulated the expression of stress marker genes including HSP70, HSP90, and HMGCR, as compared to the control (p
- Published
- 2013
28. Effects of Housing Systems on Physiological and Immunological Parameters in Laying Hens
- Author
-
Sea Hwan Sohn, Sung-Young Kang, In-Surk Jang, Yang-Soo Moon, and Young-Hyun Ko
- Subjects
medicine.medical_specialty ,Ecology ,Veterinary (miscellaneous) ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,chemistry.chemical_compound ,Endocrinology ,Animal science ,Plasma cholesterol ,chemistry ,Corticosterone ,Internal medicine ,medicine ,Animal Science and Zoology ,Bursa of Fabricius ,Cage ,Food Science - Abstract
The aim of this study was to assess the effects of housing systems on physiological and immunological responses as stress indicators in laying hens. A total of 500 White Leghorn aged 16 weeks were allotted into ten conventional cages (10 birds/cage and 810 cm 2 /bird) and four floor pens (100 birds/pen and 2,800 cm 2 /bird) for 24 weeks. The hens housed in conventional cages with higher stocking density resulted in a significantly (P
- Published
- 2013
29. The Effects of Dietary Supplementation of Vitamin C and E on the Growth Performance and the Stress Response in Broiler Chickens
- Author
-
Eun Jung Cho, Sea Hwan Sohn, In Surk Jang, and Yang Soo Moon
- Subjects
medicine.medical_specialty ,Vitamin C ,DNA damage ,Vitamin E ,medicine.medical_treatment ,Broiler ,In situ hybridization ,Biology ,Hsp70 ,Comet assay ,Endocrinology ,Internal medicine ,medicine ,medicine.symptom ,Weight gain - Abstract
Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju 660-758, KoreaABSTRACT This study was performed to investigate the investigated effects of dietary supplementation of vitamin C and E on the growth performance and stress response in broiler chickens. Stress response was analyzed by the quantity of telomeric DNA, the rate of DNA damage and the expression levels of heat shock proteins (HSPs) and hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) genes on tissues and blood. The telomere length and telomere shortening rates were analyzed by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes and tissues. The DNA damage rate of lymphocytes was quantified by the comet assay. The expression levels of HSP70, HSP90s and HMGCR genes were measured by quantitative real-time polymerase chain reaction in lymphocytes. In results, there was no significant difference among treatments in body weight, weight gain, feed intake and mortality. The telomere shortening rate of the lymphocytes was significantly lower in the vitamin E supplemented group than the control group. The DNA damage was also decreased supplemented with vitamin C and E, as compared to the control group. The vitamin E supplemented group had a significant positive effect on the expressions of HMGCR, HSP90-α and HSP90-β in lymphocytes, but had no significance on HSP70, as compared to the control group. We concluded that the dietary supplementation of vitamin E (100 mg/kg feed) had reduced the individual physiological stress response without stunt growth in broiler chickens.(Key words : vitamin C, vitamin E, growth performance, stress, broiler)
- Published
- 2013
30. Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts
- Author
-
Yang Soo Moon
- Subjects
Expression vector ,Adipogenesis ,Chemistry ,Transdifferentiation ,Cancer research ,Myocyte ,Ectopic expression ,Transfection ,Receptor ,Transcription factor ,Cell biology - Abstract
The present study was conducted to investigate whether myoblasts can be transdifferentiated into adipocytes by ectopic expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein-α (C/EBPα), sterol regulatory element binding protein-1c (SREBP1c), and Krueppel-like factor 5 (KLF5), in primary bovine satellite cells. Transcription factors were transiently transfected into primary bovine myoblasts, and the cells were cultured with adipogenic differentiation medium for 2 days and then cultured on growth medium for an additional 8 days. Ectopic expression of PPARγ or C/EBPα alone was insufficient to induce adipogenesis in myoblasts. However, overexpression of both PPARγ and C/EBPα in myoblasts was able to induce adipogenic transdifferentiation as indicated by the appearance of mature adipocytes, the induction of adipogenic gene expressions, and the suppression of myogenic gene expressions. In addition, KLF5 and PPARγ co-transfected bovine myoblasts were converted to adipocytes but not in cells transfected with only KLF5 expression vector. Overexpression of SREBP1c alone was sufficient to induce transdifferentiation from myoblasts into adipocytes. These results demonstrate that primary bovine satellite cells can be transdifferentiated into adipocytes either by single ectopic expression or combined expression of adipogenic transcription factors in a culture system.
- Published
- 2012
31. Effects of Dietary Supplementation of Coffee Meal on Growth Performance, Blood Biochemical Profiles and Antioxidant Defense System in Broiler Chickens
- Author
-
In-Surk Jang, Yang-Soo Moon, Sea Hwan Sohn, Young-Hyun Ko, and Chang-Yong Jung
- Subjects
chemistry.chemical_classification ,Meal ,Antioxidant ,medicine.medical_treatment ,Glutathione peroxidase ,Broiler ,Glutathione ,Biology ,Feed conversion ratio ,Superoxide dismutase ,Prunus ,chemistry.chemical_compound ,Horticulture ,chemistry ,medicine ,biology.protein ,Food science - Abstract
To investigate the effect of dietary supplementation of freeze-dried plum (Prunus mume Siebold and Zucc., PMS) or omija meal (Schizandra chinensis Baill.; SCB) on growth performance, organ weights, blood biochemical profiles and antioxidant defense system, a total of 96, 3-day-old male broiler chickens were assigned to three dietary groups: (1) control diet, (2) control diet supplemented with PMS at 0.2%, (3) control diet supplemented with SCB at 0.2%. In vitro antioxidant activity, plum and omija extracts showed a significantly higher radical scavenging activity (RSA). In particular, omija extract showed much higher RSA than plum extract. Dietary addition of plum or omija did not affect body weight, feed intake, feed conversion and the relative weight of digestive organ in birds. Plasma triglyceride significantly (P
- Published
- 2012
32. Effect of Dietary Supplementation of Plum or Omija on Growth Performance, Blood Biochemical Profiles and Antioxidant Defense System in Broiler Chickens
- Author
-
Young-Hyun Ko, Yang-Soo Moon, Sea-Hwan Sohn, Chang-Yong Jung, and In-Surk Jang
- Published
- 2012
33. Effects of Cyclic Heat Stress or Vitamin C Supplementation during Cyclic Heat Stress on HSP70, Inflammatory Cytokines, and the Antioxidant Defense System in Sprague Dawley Rats
- Author
-
Sea Hwan Sohn, Yang-Soo Moon, In-Surk Jang, and Seo-Hyun Yun
- Subjects
Male ,medicine.medical_specialty ,Hot Temperature ,Antioxidant ,medicine.medical_treatment ,Ascorbic Acid ,Antioxidants ,General Biochemistry, Genetics and Molecular Biology ,Immunoenzyme Techniques ,Rats, Sprague-Dawley ,Superoxide dismutase ,Random Allocation ,chemistry.chemical_compound ,Heat shock protein ,Internal medicine ,medicine ,Animals ,HSP70 Heat-Shock Proteins ,RNA, Messenger ,chemistry.chemical_classification ,General Veterinary ,Vitamin C ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Glutathione peroxidase ,General Medicine ,Glutathione ,Malondialdehyde ,Rats ,Hsp70 ,Endocrinology ,chemistry ,Organ Specificity ,Dietary Supplements ,biology.protein ,Cytokines ,Animal Science and Zoology ,Heat-Shock Response - Abstract
A total of 21 male SD rats were divided into three groups to investigate the effects of consecutive cyclic heat stress or vitamin C under heat stress on heat shock protein (HSP) 70, inflammatory cytokines, and antioxidant systems. The heat stress (HS) and vitamin C supplementation during heat stress (HS+VC) groups were exposed to cyclic heat stress (23 to 38 to 23°C) for 2 h on each of seven consecutive days. The HS+VC group had free access to water containing 0.5% vitamin C throughout the experiment. Hepatic HSP70 mRNA in the HS group was significantly (P
- Published
- 2012
34. Effects of the Combined Stress Induced by Stocking Density and Feed Restriction on Hematological and Cytokine Parameters as Stress Indicators in Laying Hens
- Author
-
Sea Hwan Sohn, Sun-Young Kang, Yang-Soo Moon, Young-Hyun Ko, and In-Surk Jang
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Stressor ,Stress induced ,Interleukin ,Spleen ,Biology ,Nitric oxide synthase ,medicine.anatomical_structure ,Cytokine ,Endocrinology ,Stocking ,Internal medicine ,medicine ,biology.protein ,Animal Science and Zoology ,Tumor necrosis factor alpha ,Food Science - Abstract
A study was conducted to investigate the effects of the combined stressor induced by high stocking density with feed restriction on immunological parameters such as leukocyte differential counts and cytokine expression in laying hens. A total of forty White Leghorn laying hens were randomly allotted into the control (12 kg of body weight/m) and the stress (44 kg of body weight/m) groups, and then birds of the stress group were given 75% of voluntary intake of the control birds for 12-d on a daily basis. There was a significant decrease in body weight without affecting the relative weights of the liver and spleen after 12-d of the combined stressor. In hematological values, no significant difference in leukocyte differential counts including heterophils (H), lymphocytes (L), monocytes and H:L ratio was observed between the two groups. In cytokines, hepatic lipopolysaccharide-induced tumor necrosis factor-α (LITNFα) and inducible nitric oxide synthase (iNOS) expression levels in the stress group were significantly (p
- Published
- 2011
35. Serum Lipids Can Convert Bovine Myogenic Satellite Cells to Adipocytes
- Author
-
Jagadish Beloor, Hye Kyeong Kang, and Yang Soo Moon
- Subjects
medicine.medical_specialty ,biology ,Transdifferentiation ,Troglitazone ,Molecular biology ,chemistry.chemical_compound ,Blood serum ,Endocrinology ,Blood chemistry ,chemistry ,Adipogenesis ,Internal medicine ,Lipid droplet ,Adipocyte ,medicine ,biology.protein ,Animal Science and Zoology ,Bovine serum albumin ,Food Science ,medicine.drug - Abstract
Serum lipid (SL) is a commercially available cholesterol-rich, proteinaceous compound extracted from bovine serum. Here we investigated the adipogenic transdifferentiation potential of SL on bovine myogenic satellite cells. Exposure of satellite cells to SL could generate lipid droplets on day 2, and further exposure to SL increased cytoplasmic lipid accumulation giving adipocyte morphology. The expression analysis of PPAR gamma and GPDH adipocyte markers along with Oil-red-O staining results confirmed the transdifferentiation potential of SL. When cells were treated at different concentrations (5, 10, 20, 40 μl/ml) of SL, the results indicated that even levels as low as 5 μl SL /ml could induce transdifferentiation, and maximum induction was obtained at 20 μl SL/ml. After treatment with SL at different concentrations the expression levels of PPAR gamma varied significantly (p
- Published
- 2010
36. Effect of Dietary Supplementation of Acanthopanax senticosus and Eucommia ulmoides on Antioxidant Defense System in Laying Hens
- Author
-
Min-Hee Lee, Young-Hyun Ko, In-Surk Jang, Sun-Young Kang, Yang-Soo Moon, and Sea Hwan Sohn
- Subjects
chemistry.chemical_classification ,medicine.medical_specialty ,Antioxidant ,biology ,ved/biology ,Glutathione peroxidase ,medicine.medical_treatment ,ved/biology.organism_classification_rank.species ,Eucommia ulmoides ,Small intestine ,Enzyme assay ,Lipid peroxidation ,Superoxide dismutase ,chemistry.chemical_compound ,Endocrinology ,medicine.anatomical_structure ,Animal science ,chemistry ,Internal medicine ,medicine ,biology.protein ,Dietary supplementation - Abstract
To investigate the effect of dietary supplementation of Acanthopanax senticosus (AS) and Eucommia ulmoides (EU) on antioxidant defense system in laying hens, a total of three hundreds sixty 20-wk old Hyline brown commercial laying hens were assigned to five dietary groups for 10-wk: (1) control diet, (2) control diet supplemented with AS at 0.5%, (3) control diet supple- mented with AS at 1.0%, (4) control diet supplemented with EU at 0.5% and (5) control diet supplemented with EU at 1.0%. Total antioxidant status (TAS) in blood and antioxidant enzymes including superoxide dismutase (SOD), gluthathione -S- transferase (GST) and glutathione peroxidase (GSH-Px), and lipid peroxidation in the small intestine and liver were measured. There were no changes in body weight for 10-wk dietary treatment. TAS in blood significantly (P
- Published
- 2010
37. The Effect of Stocking Density on Stress Related Genes and Telomeric Length in Broiler Chickens
- Author
-
Vinod Kumar Subramani, Sea Hwan Sohn, In-Surk Jang, Yeon Ju Kim, Jagadish Beloor, Hye Kyeong Kang, and Yang Soo Moon
- Subjects
Genetics ,business.industry ,Broiler ,Biology ,Poultry farming ,Body weight ,Feed conversion ratio ,Hsp70 ,Stocking ,Animal science ,medicine ,Animal Science and Zoology ,Growth rate ,medicine.symptom ,business ,Weight gain ,Food Science - Abstract
To be economically profitable, the poultry industry demands an increase in stocking density, which could adversely affect chicken welfare. The current study was performed to investigate the effect of stocking density on stress-related, heat shock protein genes (HSP70 and HSP90), 3-hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) gene and telomere length in broiler chickens. Seven-day-old broiler chickens were housed at High (0.0578 m 2 /bird), Standard (0.077 m 2 /bird) and Low (0.116 m 2 /bird) stocking densities with 8 replicates each until 35 d of age. The growth performance, such as body weight gain and average daily feed intake, was found to be significantly (p
- Published
- 2010
38. Analysis of Lipogenic and Myogenic Gene Expressions in Energy Restricted Broiler Chickens
- Author
-
Yang Soo Moon
- Subjects
Muscle tissue ,medicine.medical_specialty ,biology ,Cholesterol ,Broiler ,Sterol regulatory element-binding protein ,chemistry.chemical_compound ,Fatty acid synthase ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,Gene expression ,medicine ,biology.protein ,Gene ,Myogenin - Abstract
Department of Animal Science and Biotechnology, Jinju National University, Jinju 660-758, KoreaABSTRACT The present study was conducted to determine the effect of energy restricted (ER) diet on the expressions of lipogenic genes in liver and myogenic genes in muscle tissue of broiler chickens. Energy restriction was accomplished by providing chicks with 70% (ER70) or 85% (ER85) energy level of control diet intake. Energy restricted groups of chickens were restricted for 7 days, starting at 8 days of age. Ad libitum feeding was resumed after the restriction period, and continued through the end of the experiment. The body weight of chickens in the restricted groups gained less during the energy restriction period (P
- Published
- 2009
39. Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes
- Author
-
Jagadish Beloor, Cheol-Heui Yun, Hye Kyeong Kang, Sang Hoon Kim, and Yang Soo Moon
- Subjects
medicine.medical_specialty ,Chemistry ,Lysine ,Troglitazone ,complex mixtures ,Staining ,chemistry.chemical_compound ,Endocrinology ,Adipogenesis ,Internal medicine ,Adipocyte ,medicine ,Molecular mechanism ,bacteria ,Oil Red O ,Animal Science and Zoology ,Food Science ,medicine.drug - Abstract
The molecular mechanism of adipocyte differentiation has been well documented. However, the effect of specific nutrients such as lysine on adipocyte differentiation is poorly understood especially in ruminant animals. Therefore, the aim of the present study was to elucidate the influence of lysine on adipocyte differentiation and adipogenic genes in cultured bovine preadipocytes. The preadipocytes were treated with different concentrations of lysine (40, 160, 320 mg/L) or troglitazone (10 μM) for 2 days and then subsequently cultured in differentiation medium until day 6. Expression levels of C/EBPα were significantly higher (p
- Published
- 2009
40. Effect of Qualitative and Quantitative Feed Restriction on Growth Performance and Immune Function in Broiler Chickens
- Author
-
Sea Hwan Sohn, Sun-Young Kang, Yang-Soo Moon, I. S. Jang, and Young-Hyun Ko
- Subjects
Calorie ,Significant difference ,Broiler ,Albumin ,Biology ,Feed conversion ratio ,Basal (phylogenetics) ,Immune system ,Animal science ,Immunology ,medicine ,Animal Science and Zoology ,medicine.symptom ,Weight gain ,Food Science - Abstract
The current study was conducted to investigate the effect of early feed restriction (FR, 8 to 14 d of age) on growth performance and immune function in broiler chickens. Birds were fed corresponding diets from 3 to 35 d of age, which consisted of three phases: starter (3 to 7 d of age), FR (8 to 14 d of age) and re-alimentation (15 to 35 d of age) phases. During the FR period, each group of birds was fed the basal diet ad libitum (CON), 85% (EN85) and 70% (EN70) of lower calorie diet ad libitum (qualitative FR), and 85% (FI85) and 70% (FI70) of voluntary intake on a daily basis (quantitative FR). As a result, there was no statistical difference in weight gain, feed intake and feed conversion ratio between the CON and quantitative or qualitative FR groups during the entire (3 to 35 d) periods. In particular, the EN85 group resulted in a significant (p
- Published
- 2009
41. Transcriptional activation of Cidec by PPARγ2 in adipocyte
- Author
-
Cheol H. Yun, Yang Soo Moon, Tae Ryong Lee, Si Young Cho, Sang Hoon Kim, and Yoon-Jin Kim
- Subjects
Transcriptional Activation ,Biophysics ,Peroxisome proliferator-activated receptor ,Biology ,Biochemistry ,Mice ,chemistry.chemical_compound ,3T3-L1 Cells ,Lipid droplet ,Adipocyte ,Adipocytes ,Transcriptional regulation ,Animals ,Electrophoretic mobility shift assay ,Promoter Regions, Genetic ,Molecular Biology ,chemistry.chemical_classification ,Reporter gene ,Adipogenesis ,Binding Sites ,Base Sequence ,Proteins ,Cell Biology ,Molecular biology ,PPAR gamma ,chemistry ,Chromatin immunoprecipitation - Abstract
Cidec is a lipid droplet-associated protein, which inhibits lipolysis, leading to the accumulation of triglycerides in adipocytes. However, the transcriptional regulation of Cidec in adipocyte remains unknown. In the present study we investigated that the mouse Cidec transcript is regulated by PPARgamma2. After the differentiation of adipocyte, the expression pattern of Cidec was similar to that of PPARgamma2. In the presence of a PPARgamma agonist, the level of Cidec mRNA was highly increased. In addition, putative PPRE sites were identified in the Cidec promoter. By chromatin immunoprecipitation assay and reporter assay, we observed the binding of PPARgamma2 to the promoter of Cidec. Gel shift assay and the mutagenesis study were showed that the -219/-207 region of the Cidec promoter could function as a PPRE of the Cidec promoter. These results suggest that PPARgamma2 is required for the transcriptional activity of Cidec during adipogenesis, which could be contributed to understand the molecular mechanism of lipid droplet formation in adipocytes.
- Published
- 2008
42. Immunomodulatory effect of resistin in human dendritic cells stimulated with lipoteichoic acid from Staphylococcus aureus
- Author
-
Seung Hyun Han, Kwang-Keun Cho, Sang Hoon Kim, Cheol-Heui Yun, Sung Min Ahn, Mi Seon Jang, Young Min Son, and Yang Soo Moon
- Subjects
Lipopolysaccharides ,Staphylococcus aureus ,endocrine system diseases ,medicine.medical_treatment ,T cell ,CD14 ,Biophysics ,Adipokine ,Adipose tissue ,chemical and pharmacologic phenomena ,Biology ,Biochemistry ,Th2 Cells ,medicine ,Humans ,Resistin ,Molecular Biology ,Cells, Cultured ,Interleukin 4 ,Antigen Presentation ,nutritional and metabolic diseases ,Cell Differentiation ,Dendritic Cells ,Cell Biology ,Th1 Cells ,respiratory system ,Endocytosis ,Teichoic Acids ,Cytokine ,medicine.anatomical_structure ,Immunology ,Cytokines ,Lipoteichoic acid ,Immunosuppressive Agents ,hormones, hormone substitutes, and hormone antagonists - Abstract
Resistin is an adipokine whose physiologic role in obesity, type II diabetes mellitus, and inflammatory diseases has been a subject of debate because while it is expressed in adipocytes and adipose tissue in mouse, it is expressed in leukocytes, such as macrophages, in human. In the present study, we attempt to define the effect of resistin on human dendritic cells (DCs) derived from CD14 + monocytes. When DCs were stimulated with lipoteichoic acid (LTA) and treated with various concentrations of resistin, antigen-uptake process and the endocytic capacity of DCs were decreased. It is intriguing that resistin attenuated cytokine production in LTA-primed DCs. Consequently, T cell activity was reduced when lymphocytes were mixed with Staphylococcus aureus -primed autologous DCs treated with resistin compared to S. aureus -primed DCs without resistin. Our results suggest that resistin interferes with the efficacy of immune responses activated by Gram-positive bacterial infection in human DCs.
- Published
- 2008
43. Procaryotic Expression of Porcine Acid-Labile Subunit of the 150-kDa Insulin-like Growth Factor Complex
- Author
-
Yang-Soo Moon, Hye-Kyeong Kang, and C. Young Lee
- Subjects
Insulin-like growth factor ,ACID-LABILE SUBUNIT ,Ecology ,Veterinary (miscellaneous) ,medicine.medical_treatment ,Mutagenesis ,medicine ,Animal Science and Zoology ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Molecular biology ,Food Science - Abstract
Acid-labile subunit(ALS)는 85-kDa 크기의 당단백질로서 7.5-kDa의 insulin-like growth factor(IGF) 및 40~45-kDa IGF-binding protein-3와 결합하여 150-kDa ternary complex를 형성하는 혈장단백질이다. 선행연구에서 본 연구진은 reverse transcription-polymerase chain reaction(RT-PCR) 방법으로 돼지(porcine) ALS(pALS)의 coding sequence를 합성하여 plasmid vector에 삽입시켜 ‘expression construct’를 제작한 바 있다. 그러나 본 expression construct의 pALS coding sequence에는 PCR error로 추정되는 원인으로 말미암아 2개의 bases에서 mis-sense mutation이 일어난 것이 발견되었다. 본 연구에서는 ‘site-directed mutagenesis’ 방법으로 pALS의 올바른 coding sequence를 합성하여 ‘insert DNA’의 마지막 codon 다음에 ‘His-tag’ sequence가 위치한 pET- 28a(+) plasmid expression vector에 삽입하였다. 본 expression construct는 E. coli BL21(DE3) 세포에서 ‘induction’ 시켰고, 발현된 유전자재조합(recombinant) peptide는 Ni-affinity chromato- graphy로 정제하였다. 이렇게 affinity chro- matography로 정제된 peptide는 SDS-PAGE에서 66kDa 위치에 single band를 나타냄으로써 recombinant pALS의 예상된 질량과 일치하였다. 이상의 결과는 본 연구에서 recombinant pALS peptide가 성공적으로 발현정제되었음을 시사한다. 【Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.】
- Published
- 2008
44. Characteristics of Structure and Expression Pattern of ADSF/resistin Gene in Korean Native Cattle Korea
- Author
-
Sang Hoon Kim, Yun Jai Choi, Ji Ae Park, Kang Seok Seo, Hye Kyeong Kang, and Yang Soo Moon
- Subjects
medicine.medical_specialty ,Adipose tissue ,Biology ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Adipogenesis ,Adipocyte ,Internal medicine ,Complementary DNA ,Gene expression ,Hanwoo ,medicine ,Animal Science and Zoology ,Resistin ,Gene ,Food Science - Abstract
Adipocyte-specific secretory factor (ADSF)/resistin, a hormone, is a small cysteine-rich protein secreted from adipose tissue and has been implicated in modulating adipogenesis in humans and rodents. The objective of this study was to clone a gene encoding ADSF/resistin and to characterize its function in Korean Native Cattle (Hanwoo). The coding sequence was 330 base pairs and it encoded a protein of 109 amino acids. An NCBI BLAST-search revealed the cloned cDNA fragment shared significant homology (82%) with the cDNA encoding the human ADSF/resistin. The nucleotide sequence homology of the Hanwoo sequence was 73% and 64% for the rat and mouse, respectively. A 654 bp ADSF/resistin gene promoter was cloned and putative binding sites of transcription factors were identified. Tissue distribution of ADSF mRNA was examined in liver, skeletal muscles (tenderloin, biceps femoris), subcutaneous fat, and perirenal fat by RT-PCR. ADSF mRNAs were detected in fat tissues but not in liver and muscles, suggesting that ADSF/resistin expression may be induced during adipogenesis. Although, the physiological function of ADSF/resistin in the cow remains to be determined, these data indicate ADSF is related to the adipocyte phenotype and may have a possibly regulatory role in adipocyte function.
- Published
- 2006
45. Effect of Maternal Passive Autoimmunization against Myostatin on Growth Performance in Chickens
- Author
-
Xun Jin, Yun-Jaie Choi, Zhongshan Hong, Yang-Soo Moon, Dong Il Jin, Sung Chan Kim, S.K. You, Jae Yong Han, Y.H. Yin, Jun-Hyeon Cho, and Hyang-Sim Lee
- Subjects
medicine.medical_specialty ,Period (gene) ,Broiler ,Skeletal muscle ,Myostatin ,Biology ,Hyperplasia ,musculoskeletal system ,medicine.disease ,Negative regulator ,Muscle hypertrophy ,Endocrinology ,medicine.anatomical_structure ,Immunization ,Internal medicine ,medicine ,biology.protein ,Animal Science and Zoology ,Food Science - Abstract
Myostatin is a negative regulator of skeletal muscle growth and a loss of functional myostatin protein increases muscle hypertrophy and hyperplasia in cattle. The present study was conducted to investigate whether maternal passive immunization against myostatin would improve growth performance in chickens. A complete broiler myostatin cDNA was cloned and it was expressed into two transcripts as 1,128 bp and 985 bp by alternative splicing. A conjugated mature myostatin (350 bp) was used to induce autoimmunization and maternal passively immunized chickens was used for the experiment. It was confirmed that there was a maternal passive immunization against myostatin at zero weeks of age, but its effect was reduced by 6 weeks of age. The auto-immunized groups showed smaller body weights than those of control group during the growing period and the difference was getting bigger with time until 6 weeks of age. These results suggest that passive autoimmunization against myostatin used in this study is not potent enough to stimulate growth performance in chickens. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 7 : 1017-1021)
- Published
- 2005
46. Expression of Clostridium thermocellum Endoglucanase Gene in Lactobacillus bulgaricus and Lactobacillus plantarum and in vitro Survival Characteristics of the Transformed Lactobacilli
- Author
-
Seong Ho Kang, J.H. Woo, C.J. Yang, J.S. Cho, Yun-Jaie Choi, Hyun Jung Lee, Hong Gu Lee, and Yang-Soo Moon
- Subjects
Expression vector ,Ecology ,Veterinary (miscellaneous) ,food and beverages ,Kanamycin ,Cellulase ,Biology ,equipment and supplies ,biology.organism_classification ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Microbiology ,fluids and secretions ,Streptomycin ,Lactobacillus ,Colistin ,medicine ,biology.protein ,bacteria ,Clostridium thermocellum ,Animal Science and Zoology ,Lactobacillus plantarum ,Food Science ,medicine.drug - Abstract
Endoglucanase A from Clostridium thermocellum which is resistant to pancreatic proteinase was selected out of numbers cellulases then were expressed in lactobacilli. Recombinant lactobacilli expression vector, pSD1, harboring the endoglucanase gene from C. thermocellum under the control of its own promoter, was constructed. Both L. bulgaricus and L. plantarum were electrotransformed with pSD1. The endoglucanase activities of 0.120 and 0.144 U/ml were found in culture media of L. bulgaricus and L. plantarum containing pSD1, respectively. In vitro survival characteristics of the transformed lactobacilli were tested. Both L. bulgaricus and L. plantarum showed a similar resistance to low pH 3. Moreover, L. plantarum was bile-salt resistant in the presence of 0.3 and 1% oxgall. L. bulgaricus and L. plantarum showed a rather homogenous resistant pattern against the tested antibiotics. Both of the strains were resistant to amikacin, gentamicin, streptomycin, kanamycin, and colistin.
- Published
- 2003
47. Occupancy and Function of the −150 Sterol Regulatory Element and −65 E-Box in Nutritional Regulation of the Fatty Acid Synthase Gene in Living Animals
- Author
-
Hei Sook Sul, Yang Soo Moon, María Jesús Latasa, Chulho Kang, and Michael J. Griffin
- Subjects
Time Factors ,Transcription, Genetic ,Transgene ,Mice, Transgenic ,E-box ,Biology ,Models, Biological ,Gene Expression Regulation, Enzymologic ,Mice ,Ribonucleases ,Genes, Reporter ,Formaldehyde ,Animals ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,Transcriptional Regulation ,Regulation of gene expression ,Reporter gene ,Binding Sites ,Models, Genetic ,Cell Biology ,Precipitin Tests ,Molecular biology ,Chromatin ,Sterol regulatory element-binding protein ,DNA-Binding Proteins ,Fatty acid synthase ,Cross-Linking Reagents ,Mutation ,CCAAT-Enhancer-Binding Proteins ,biology.protein ,RNA ,Sterol Regulatory Element Binding Protein 1 ,Fatty Acid Synthases ,Gene Deletion ,Plasmids ,Protein Binding ,Transcription Factors - Abstract
12 pages, 7 figures., Upstream regulatory factor (USF) and sterol regulatory element binding protein (SREBP) play key roles in the transcriptional regulation of the fatty acid synthase (FAS) gene by feeding and insulin. Due to the dual binding specificity of SREBP, as well as the presence of multiple consensus sites for these transcription factors in the FAS promoter, their physiologically relevant functional binding sites have been controversial. Here, in order to determine the occupancy of the putative USF and SREBP binding sites, we examined their protein-DNA interactions in living animals by using formaldehyde cross-linking and immunoprecipitation of chromatin and tested the function of these elements by employing mice transgenic for a reporter gene driven by various 5' deletions as well as site-specific mutations of the FAS promoter. We show that the -332 and -65 E-boxes are bound by USF in both fasted and refed mice, while the -150 SRE is bound by SREBP-1 only in refed mice. We also found that mutation of either the -150 SRE or the -65 E-box abolishes the feeding-induced activation of the FAS promoter in transgenic mice. Furthermore, in vivo occupancy of the FAS promoter by SREBP in the fed state can be prevented by mutation not only of the -150 SRE but, unexpectedly, of the -65 E-box as well. We conclude that the FAS promoter is activated during refeeding via the induced binding of SREBP to the -150 SRE and that USF binding to the -65 E-box is also required for SREBP binding and activation of the FAS promoter., The work was supported by NIH grant DK36264 to H.S.S.
- Published
- 2003
48. Molecular Cloning of Adipose Tissue-Specific Genes by cDNA Microarray
- Author
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Kee-Hong Kim and Yang Soo Moon
- Subjects
Sequence analysis ,Adipose tissue ,3T3-L1 ,Biology ,Molecular biology ,chemistry.chemical_compound ,chemistry ,Complementary DNA ,Adipocyte ,Gene expression ,Animal Science and Zoology ,Northern blot ,Gene ,Food Science - Abstract
In an attempt to isolate novel molecules that may play a regulatory role in adipocyte differentiation, we devised an experimental strategy to identify adipose tissue-specific genes by modifying cDNA microarray technique. We used genefilter membranes containing approximately 15,000 rat non-redundant EST clones of which 4,000 EST were representative clones of known genes and 11,000 ESTs were uncharacterized clones. A series of hybridization of genefilter membranes with cDNA probes prepared from various rat tissues and nucleic acids sequence analysis allowed us to identify two adipose-tissue specific genes, adipocyte-specific secretory factor (ADSF) and H-rev107. Verification of tissue-specific expression patterns of these two genes by Northern blot analysis showed that ADSF mRNA is exclusive expressed in adipose tissue and the H-rev107 mRNA is predominantly expressed in adipose tissue. Further analysis of gene expression of ADSF and H-rev107 during 3T3-L1 adipocyte differentiation revealed that the ADSF and H-rev107 gene expression patterns are closely associated with the adipocyte differentiation program, indicating their possible role in the regulation of adipose tissue development. Overall, we demonstrated an application of modified cDNA microarray technique in molecular cloning, resulting in identification of two novel adipose tissue-specific genes. This technique will also be used as a useful tool in identifying novel genes expressed in a tissue-specific manner. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 12 : 1837-1841)
- Published
- 2003
49. Reversine increases the plasticity of lineage-committed preadipocytes to osteogenesis by inhibiting adipogenesis through induction of TGF-β pathway in vitro
- Author
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Dae-Hee Lee, Yang Soo Moon, Jeong Geun Park, and Kee-Hong Kim
- Subjects
medicine.medical_specialty ,Cell Survival ,Morpholines ,Biophysics ,Adipose tissue ,Apoptosis ,Cell morphology ,Biochemistry ,chemistry.chemical_compound ,Mice ,Osteogenesis ,Transforming Growth Factor beta ,Internal medicine ,3T3-L1 Cells ,medicine ,Adipocytes ,Animals ,Humans ,Cell Lineage ,Molecular Biology ,Adipogenesis ,Chemistry ,Regeneration (biology) ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,Cell Dedifferentiation ,In vitro ,Cell biology ,Endocrinology ,Purines ,Cell Transdifferentiation ,Reversine ,Transforming growth factor ,Signal Transduction - Abstract
Reversine has been reported to reverse differentiation of lineage-committed cells to mesenchymal stem cells (MSCs), which then enables them to be differentiated into other various lineages. Both adipocytes and osteoblasts are known to originate from common MSCs, and the balance between adipogenesis and osteogenesis in MSCs is reported to modulate the progression of various human diseases, such as obesity and osteoporosis. However, the role of reversine in modulating the adipogenic potential of lineage-committed preadipocytes and their plasticity to osteogenesis is unclear. Here we report that reversine has an anti-adipogenic function in 3T3-L1 preadipocytes in vitro and alters cell morphology and viability. The transforming growth factor-β (TGF-β) pathway appears to be required for the anti-adipogenic effect of reversine, due to reversine-induced expression of genes involved in TGF-β pathway and reversal of reversine-inhibited adipogenesis by inhibition of TGF-β pathway. We show that treatment with reversine transformed 3T3-L1 preadipocytes into MSC-like cells, as evidenced by the expression of MSCs marker genes. This, in turn, allowed differentiation of lineage-committed 3T3-L1 preadipocytes to osteoblasts under the osteogenic condition in vitro. Collectively, these findings reveal a new function of reversine in reversing lineage-committed preadipocytes to osteogenesis in vitro, and provide new insights into adipose tissue-based regeneration of osteoblasts.
- Published
- 2014
50. Nutritional regulation of the fatty acid synthase promoter in vivo : Sterol regulatory element binding protein functions through an upstream region containing a sterol regulatory element
- Author
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Maria-Jesus Latasa, Yang Soo Moon, Hei Sook Sul, and Kee-Hong Kim
- Subjects
Chloramphenicol O-Acetyltransferase ,Transcriptional Activation ,Mice, Transgenic ,Gene Expression Regulation, Enzymologic ,Upstream Stimulatory Factor ,Chloramphenicol acetyltransferase ,Mice ,Animals ,Promoter Regions, Genetic ,Transcription factor ,DNA Primers ,Multidisciplinary ,Base Sequence ,biology ,Ccaat-enhancer-binding proteins ,Nuclear Proteins ,Promoter ,3T3 Cells ,Biological Sciences ,Molecular biology ,Sterol regulatory element-binding protein ,DNA-Binding Proteins ,Fatty acid synthase ,Food ,Starvation ,CCAAT-Enhancer-Binding Proteins ,biology.protein ,Sterol Regulatory Element Binding Protein 1 ,Fatty Acid Synthases ,Transcription Factors - Abstract
The transcription of fatty acid synthase (FAS), a central enzyme in de novo lipogenesis, is dramatically induced by fasting/refeeding and insulin. We reported that upstream stimulatory factor binding to the −65 E-box is required for induction of the FAS transcription by insulin in 3T3-L1 adipocytes. On the other hand, we recently found that two upstream 5′ regions are required for induction in vivo by fasting/refeeding and insulin; one at −278 to −131 albeit at a low level, and the other at −444 to −278 with an E-box at −332 where upstream stimulatory factor functions for maximal induction. Here, we generated double transgenic mice carrying the chloramphenicol acetyltransferase reporter driven by the various 5′ deletions of the FAS promoter region and a truncated active form of the sterol regulatory element (SRE) binding protein (SREBP)-1a. We found that SREBP participates in the nutritional regulation of the FAS promoter and that the region between −278 and −131 bp is required for SREBP function. We demonstrate that SREBP binds the −150 canonical SRE present between −278 and −131, and SREBP can function through the −150 SRE in cultured cells. These in vivo and in vitro results indicate that SREBP is involved in the nutritional induction of the FAS promoter via the −278/−131 region and that the −150 SRE is the target sequence.
- Published
- 2000
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