29 results on '"Yanglin Ding"'
Search Results
2. Surviving and thriving: How plants perceive and respond to temperature stress
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Yanglin, Ding and Shuhua, Yang
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Cold Temperature ,Crops, Agricultural ,Stress, Physiological ,Temperature ,Plant Development ,Cell Biology ,Molecular Biology ,Heat-Shock Response ,General Biochemistry, Genetics and Molecular Biology ,Developmental Biology - Abstract
The dramatic temperature fluctuations spurred by climate change inhibit plant growth and threaten crop productivity. Unraveling how plants defend themselves against temperature-stress-induced cellular impairment is not only a crucial fundamental issue but is also of critical importance for agricultural sustainability and food security. Here, we review recent developments in elucidating the molecular mechanisms used by plants to sense and respond to cold and heat stress at multiple levels. We also describe the trade-off between plant growth and responses to high and low temperatures. Finally, we discuss possible strategies that could be used to engineer temperature-stress-tolerant, high-yielding crops.
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- 2022
3. Rice functional genomics: decades’ efforts and roads ahead
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Rongzhi Chen, Yiwen Deng, Yanglin Ding, Jingxin Guo, Jie Qiu, Bing Wang, Changsheng Wang, Yongyao Xie, Zhihua Zhang, Jiaxin Chen, Letian Chen, Chengcai Chu, Guangcun He, Zuhua He, Xuehui Huang, Yongzhong Xing, Shuhua Yang, Daoxin Xie, Yaoguang Liu, and Jiayang Li
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Crops, Agricultural ,Epigenomics ,Phenotype ,Food Parasitology ,Plant Growth Regulators ,Oryza ,General Agricultural and Biological Sciences ,Genome, Plant ,General Biochemistry, Genetics and Molecular Biology ,Genome-Wide Association Study ,Signal Transduction ,General Environmental Science - Abstract
Rice (Oryza sativa L.) is one of the most important crops in the world. Since the completion of rice reference genome sequences, tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks. In this review, we summarize the research progress of rice biology over past decades, including omics, genome-wide association study, phytohormone action, nutrient use, biotic and abiotic responses, photoperiodic flowering, and reproductive development (fertility and sterility). For the roads ahead, cutting-edge technologies such as new genomics methods, high-throughput phenotyping platforms, precise genome-editing tools, environmental microbiome optimization, and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice, and facilitate integrations of the knowledge for agricultural applications.
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- 2021
4. CPK28-NLP7 module integrates cold-induced Ca 2+ signal and transcriptional reprogramming in Arabidopsis
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Yanglin Ding, Hao Yang, Shifeng Wu, Diyi Fu, Minze Li, Zhizhong Gong, and Shuhua Yang
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Multidisciplinary - Abstract
Exposure to cold triggers a spike in cytosolic calcium (Ca 2+ ) that often leads to transcriptional reprogramming in plants. However, how this Ca 2+ signal is perceived and relayed to the downstream cold signaling pathway remains unknown. Here, we show that the CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) initiates a phosphorylation cascade to specify transcriptional reprogramming downstream of cold-induced Ca 2+ signal. Plasma membrane (PM)–localized CPK28 is activated rapidly upon cold shock within 10 seconds in a Ca 2+ -dependent manner. CPK28 then phosphorylates and promotes the nuclear translocation of NIN-LIKE PROTEIN 7 (NLP7), a transcription factor that specifies the transcriptional reprogramming of cold-responsive gene sets in response to Ca 2+ , thereby positively regulating plant response to cold stress. This study elucidates a previously unidentified mechanism by which the CPK28-NLP7 regulatory module integrates cold-evoked Ca 2+ signal and transcriptome and thus uncovers a key strategy for the rapid perception and transduction of cold signals from the PM to the nucleus.
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- 2022
5. Protein kinases in plant responses to drought, salt, and cold stress
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Shuhua Yang, Zhizhong Gong, Yan Guo, Yanglin Ding, Xuexue Chen, Baoshan Wang, Chun-Peng Song, and Yongqing Yang
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0106 biological sciences ,0301 basic medicine ,MAPK/ERK pathway ,Abiotic component ,Kinase ,fungi ,food and beverages ,Plant Science ,Biology ,MAPK cascade ,01 natural sciences ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Droughts ,Cell biology ,03 medical and health sciences ,Transduction (genetics) ,Focal Adhesion Kinase 2 ,030104 developmental biology ,Gene Expression Regulation, Plant ,Signal transduction ,Protein kinase A ,Cold stress ,Signal Transduction ,010606 plant biology & botany - Abstract
Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review, we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1 (SNF1)-related protein kinases (SnRKs), mitogen-activated protein kinase (MAPK) cascades, calcium-dependent protein kinases (CDPKs/CPKs), and receptor-like kinases (RLKs). We also discuss future challenges in these research fields.
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- 2021
6. RAF22, ABI1 and OST1 form a dynamic interactive network that optimizes plant growth and responses to drought stress in Arabidopsis
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Zhihui Sun, Zhenkai Feng, Yanglin Ding, Yuanpeng Qi, Shan Jiang, Zhen Li, Yu Wang, Junsheng Qi, Chunpeng Song, Shuhua Yang, and Zhizhong Gong
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Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Mutation ,Arabidopsis ,Phosphoprotein Phosphatases ,Plant Science ,Molecular Biology ,Protein Kinases ,Abscisic Acid ,Droughts - Abstract
Plants adapt to their ever-changing environment via positive and negative signals induced by environmental stimuli. Drought stress, for instance, induces accumulation of the plant hormone abscisic acid (ABA), triggering ABA signal transduction. However, the molecular mechanisms for switching between plant growth promotion and stress response remain poorly understood. Here we report that RAF (rapidly accelerated fibrosarcoma)-LIKE MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 22 (RAF22) in Arabidopsis thaliana physically interacts with ABA INSENSITIVE 1 (ABI1) and phosphorylates ABI1 at Ser416 residue to enhance its phosphatase activity. Interestingly, ABI1 can also enhance the activity of RAF22 through dephosphorylation, reciprocally inhibiting ABA signaling and promoting the maintenance of plant growth under normal conditions. Under drought stress, however, the ABA-activated OPEN STOMATA1 (OST1) phosphorylates the Ser81 residue of RAF22 and inhibits its kinase activity, restraining its enhancement of ABI1 activity. Taken together, our study reveals that RAF22, ABI1, and OST1 form a dynamic regulatory network that plays crucial roles in optimizing plant growth and environmental adaptation under drought stress.
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- 2022
7. Plant abiotic stress response and nutrient use efficiency
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Liming Xiong, Yiting Shi, Yan Guo, Jijang Li, Yu Wang, Guohua Xu, Feng Qin, Peng Yun Wang, Jian-Kang Zhu, Dai-Yin Chao, Yongqing Yang, Shuhua Yang, Luis Herrera-Estrella, Huazhong Shi, Jingrui Li, Zhizhong Gong, and Yanglin Ding
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0301 basic medicine ,Future studies ,Soil nutrients ,Plant Development ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Soil ,03 medical and health sciences ,0302 clinical medicine ,Nutrient ,Gene Expression Regulation, Plant ,Stress, Physiological ,Metals, Heavy ,Cellular ion homeostasis ,Phosphorylation ,Plant Proteins ,General Environmental Science ,Abiotic component ,Abiotic stress ,Ecology ,Stress signaling ,Plants ,030104 developmental biology ,030220 oncology & carcinogenesis ,Abiotic stress response ,Calcium Channels ,General Agricultural and Biological Sciences ,Signal Transduction ,Transcription Factors - Abstract
Abiotic stresses and soil nutrient limitations are major environmental conditions that reduce plant growth, productivity and quality. Plants have evolved mechanisms to perceive these environmental challenges, transmit the stress signals within cells as well as between cells and tissues, and make appropriate adjustments in their growth and development in order to survive and reproduce. In recent years, significant progress has been made on many fronts of the stress signaling research, particularly in understanding the downstream signaling events that culminate at the activation of stress- and nutrient limitation-responsive genes, cellular ion homeostasis, and growth adjustment. However, the revelation of the early events of stress signaling, particularly the identification of primary stress sensors, still lags behind. In this review, we summarize recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling and discuss new directions for future studies.
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- 2020
8. The direct targets of CBFs: In cold stress response and beyond
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Hao Yang, Diyi Fu, Yue Song, Shuhua Yang, Xiaoyan Zhang, Yiting Shi, Zhizhong Gong, Jian Lv, Yanglin Ding, and Minze Li
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Arabidopsis ,Plant Science ,Biochemistry ,Regulon ,General Biochemistry, Genetics and Molecular Biology ,Deep sequencing ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Cold acclimation ,Arabidopsis thaliana ,Transcription factor ,Gene ,biology ,Arabidopsis Proteins ,Cold-Shock Response ,biology.organism_classification ,Lipid Metabolism ,Cell biology ,Trans-Activators ,Carbohydrate Metabolism ,Chromatin Immunoprecipitation Sequencing ,Chromatin immunoprecipitation ,circulatory and respiratory physiology ,Transcription Factors - Abstract
Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive (COR) genes. Essential to this process is the C-repeat binding factor (CBF)-dependent pathway, involving the activity of AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) to determine the genome-wide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat (CRT)/dehydration-responsive elements (CRT/DRE; G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light, and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants.
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- 2021
9. Reciprocal regulation between the negative regulator PP2CG1 phosphatase and the positive regulator OST1 kinase confers cold response in Arabidopsis
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Zhizhong Gong, Yanglin Ding, Chun-Peng Song, Yiting Shi, Jingyan Liu, Jian Lv, Yuhang Ming, and Shuhua Yang
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0106 biological sciences ,0301 basic medicine ,Phosphatase ,Regulator ,Arabidopsis ,Plant Science ,Pyruvate dehydrogenase phosphatase ,01 natural sciences ,Biochemistry ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Dephosphorylation ,03 medical and health sciences ,Freezing ,Protein phosphorylation ,Amino Acid Sequence ,Phosphorylation ,Protein kinase A ,Chemistry ,Kinase ,Arabidopsis Proteins ,Protein Stability ,Cold-Shock Response ,Epistasis, Genetic ,Adaptation, Physiological ,Cell biology ,Cold Temperature ,Protein Phosphatase 2C ,030104 developmental biology ,Protein Kinases ,010606 plant biology & botany ,Protein Binding - Abstract
Protein phosphorylation and dephosphorylation have been reported to play important roles in plant cold responses. In addition, phospho-regulatory feedback is a conserved mechanism for biological processes and stress responses in animals and plants. However, it is less well known that a regulatory feedback loop is formed by the protein kinase and the protein phosphatase in plant responses to cold stress. Here, we report that OPEN STOMATA 1 (OST1) and PROTEIN PHOSPHATASE 2C G GROUP 1 (PP2CG1) reciprocally regulate the activity during the cold stress response. The interaction of PP2CG1 and OST1 is inhibited by cold stress, which results in the release of OST1 at the cytoplasm and nucleus from suppression by PP2CG1. Interestingly, cold-activated OST1 phosphorylates PP2CG1 to suppress its phosphatase activity, thereby amplifying cold signaling in plants. Mutations of PP2CG1 and its homolog PP2CG2 enhance freezing tolerance, whereas overexpression of PP2CG1 decreases freezing tolerance. Moreover, PP2CG1 negatively regulates protein levels of C-REPEAT BINDING FACTORs (CBFs) under cold stress. Our results uncover a phosphor/dephosphor-regulatory feedback loop mediated by PP2CG1 phosphatase and OST1 protein kinase in plant cold responses.
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- 2021
10. Studies on plant responses to environmental change in China: the past and the future
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Long Mao, Shuhua Yang, Liang Ma, ShaoJian Zheng, Jia-Shi Peng, JiMing Gong, DaiYin Chao, LiJun Luo, Yu Wang, Yiting Shi, JinSheng Lai, HongQuan Yang, Yan Guo, Yongqing Yang, ChaoFeng Huang, Zhizhong Gong, Yanglin Ding, Rongcheng Lin, XueYing Guan, Chengcai Chu, TianZhen Zhang, Caifu Jiang, Kang Chong, and Feng Qin
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Environmental change ,biology ,Abiotic stress ,Environmental protection ,Arabidopsis ,Environmental science ,Pharmacology (medical) ,Heavy metals ,China ,biology.organism_classification ,Light stress - Abstract
Chinese scientists have made remarkable achievements in plant research since the founding of the People’s Republic of China, especially in the past 40 years during the reform and opening to the outside world. In this review, we summarize the contributions of Chinese plant scientists in the field of plant abiotic stress responses, such as drought, salt, light stress, low and high temperatures, and heavy metals and aluminum poison, using Arabidopsis , rice, maize, wheat, cotton, and other crops as materials. We also discuss the major challenges in plant abiotic stress research, and put forward the possibility of designing crops that are tolerant to multiple environmental stresses.
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- 2019
11. CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis.
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Yanglin Ding, Hao Yang, Shifeng Wu, Diyi Fu, Minze Li, Zhizhong Gong, and Shuhua Yang
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CALMODULIN , *TRANSCRIPTION factors , *TRP channels , *RNA-binding proteins , *LIFE sciences , *GREEN fluorescent protein , *CALCIUM-dependent protein kinase , *ARABIDOPSIS - Abstract
The article presents a study which showed that the calcium-dependent protein kinase 28 (CKP28) initiates a phosphorylation cascade to specify transcriptional reprogramming downstream of cold-induced calcium signal. Topics discussed include important role of calcium in plant responses to cold stress and Cold-Responsive (COR) gene expression, protein expression and purification from Escherichia coli, and quantification and statistical analysis.
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- 2022
- Full Text
- View/download PDF
12. The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants
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Zhizhong Gong, Yiting Shi, Yanglin Ding, Julia M. Davies, Marc R. Knight, Chun-Peng Song, Katie A. Wilkins, Shuhua Yang, Liang Ma, Yi Wang, Qiangbo Liu, Yan Guo, Heather Knight, Wang, Yi [0000-0002-3660-5859], Wilkins, Katie A [0000-0001-6513-856X], Yang, Shuhua [0000-0003-1229-7166], and Apollo - University of Cambridge Repository
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Mutant ,Arabidopsis ,chemistry.chemical_element ,Biology ,Calcium ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Gene Expression Regulation, Plant ,calcium-permeable transporter AtANN1 ,Freezing ,Arabidopsis thaliana ,Calcium Signaling ,Molecular Biology ,030304 developmental biology ,Calcium signaling ,0303 health sciences ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Cold-Shock Response ,Cell Membrane ,calcium signal ,Transporter ,Articles ,biology.organism_classification ,freezing tolerance ,Cell biology ,Cold Temperature ,chemistry ,Phosphorylation ,OST1 kinase ,Protein Kinases ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
The transient elevation of cytosolic free calcium concentration ([Ca(2+)](cyt)) induced by cold stress is a well‐established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca(2+)‐permeable transporter ANNEXIN1 (AtANN1) mediates cold‐triggered Ca(2+) influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold‐induced [Ca(2+)](cyt) increase and upregulation of the cold‐responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca(2+) transport activity, thereby potentiating Ca(2+) signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold‐induced [Ca(2+)](cyt) elevation in the ost1‐3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1‐AtANN1 to cold‐induced Ca(2+) signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis.
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- 2020
13. Molecular Regulation of Plant Responses to Environmental Temperatures
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Yiting Shi, Yanglin Ding, and Shuhua Yang
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0106 biological sciences ,0301 basic medicine ,Plant growth ,Acclimatization ,Climate Change ,Plant Science ,Flowers ,Biology ,01 natural sciences ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Cold acclimation ,Temperate climate ,Morphogenesis ,Molecular Biology ,Plant Proteins ,fungi ,Global warming ,Temperature ,food and beverages ,Vernalization ,Plants ,Heat stress ,Horticulture ,030104 developmental biology ,Germination ,Freezing stress ,Seeds ,010606 plant biology & botany - Abstract
Temperature is a key factor governing the growth and development, distribution, and seasonal behavior of plants. The entire plant life cycle is affected by environmental temperatures. Plants grow rapidly and exhibit specific changes in morphology under mild average temperature conditions, a response termed thermomorphogenesis. When exposed to chilling or moist chilling low temperatures, flowering or seed germination is accelerated in some plant species; these processes are known as vernalization and cold stratification, respectively. Interestingly, once many temperate plants are exposed to chilling temperatures for some time, they can acquire the ability to resist freezing stress, a process termed cold acclimation. In the face of global climate change, heat stress has emerged as a frequent challenge, which adversely affects plant growth and development. In this review, we summarize and discuss recent progress in dissecting the molecular mechanisms regulating plant thermomorphogenesis, vernalization, and responses to extreme temperatures. We also discuss the remaining issues that are crucial for understanding the interactions between plants and temperature.
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- 2019
14. Molecular Regulation of CBF Signaling in Cold Acclimation
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Yanglin Ding, Shuhua Yang, and Yiting Shi
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0106 biological sciences ,0301 basic medicine ,Plant growth ,Arabidopsis Proteins ,Mechanism (biology) ,Kinase ,Arabidopsis ,food and beverages ,Plant Science ,Biology ,01 natural sciences ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Gene Expression Regulation, Plant ,Gene expression ,Cold acclimation ,Signal transduction ,Transcription factor ,Function (biology) ,Signal Transduction ,Transcription Factors ,010606 plant biology & botany - Abstract
Cold stress restricts plant growth, development, and distribution. Understanding how plants transduce and respond to cold signals has long been a topic of interest. Traditional genetic and molecular analyses have identified C-repeat/DREB binding factors (CBFs) as key transcription factors that function in cold acclimation. Recent studies revealed the involvement of pivotal protein kinases and transcription factors in CBF-dependent signaling, expanding our knowledge of cold signal transduction from perception to downstream gene expression events. In this review, we summarize recent advances in our understanding of the molecular regulation of these core components of the CBF cold signaling pathway. Knowledge of the mechanism underlying the ability of plants to survive freezing temperatures will facilitate the development of crop plants with increased freezing tolerance.
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- 2018
15. EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis
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Chun-Peng Song, Junping Gao, Yiting Shi, Jian Hua, Shuhua Yang, Zhizhong Gong, Yanglin Ding, and Jian Lv
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Acclimatization ,Phosphatase ,Arabidopsis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Fatty Acids, Monounsaturated ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Freezing ,Kinase activity ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Freezing tolerance ,030304 developmental biology ,Myristoylation ,0303 health sciences ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Cold-Shock Response ,Cell Membrane ,Articles ,biology.organism_classification ,Plants, Genetically Modified ,Cell biology ,Cold Temperature ,Enzyme Activation ,Protein Phosphatase 2C ,Binding ability ,Protein Kinases ,Protein Processing, Post-Translational ,030217 neurology & neurosurgery ,Function (biology) ,Signal Transduction - Abstract
OST1 (open stomata 1) protein kinase plays a central role in regulating freezing tolerance in Arabidopsis ; however, the mechanism underlying cold activation of OST1 remains unknown. Here, we report that a plasma membrane‐localized clade‐E growth‐regulating 2 (EGR2) phosphatase interacts with OST1 and inhibits OST1 activity under normal conditions. EGR2 is N‐myristoylated by N‐myristoyltransferase NMT1 at 22°C, which is important for its interaction with OST1. Moreover, myristoylation of EGR2 is required for its function in plant freezing tolerance. Under cold stress, the interaction of EGR2 and NMT1 is attenuated, leading to the suppression of EGR2 myristoylation in plants. Plant newly synthesized unmyristoylated EGR2 has decreased binding ability to OST1 and also interferes with the EGR2‐OST1 interaction under cold stress. Consequently, the EGR2‐mediated inhibition of OST1 activity is released. Consistently, mutations of EGRs cause plant tolerance to freezing, whereas overexpression of EGR2 exhibits decreased freezing tolerance. This study thus unravels a molecular mechanism underlying cold activation of OST1 by membrane‐localized EGR2 and suggests that a myristoyl switch on EGR2 helps plants to adapt to cold stress.
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- 2018
16. Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants
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Yanglin Ding, Yiting Shi, and Shuhua Yang
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0106 biological sciences ,0301 basic medicine ,Plant growth ,Physiology ,Cold tolerance ,Cold-Shock Response ,fungi ,food and beverages ,Plant Science ,Computational biology ,Protein degradation ,Biology ,Plants ,01 natural sciences ,Crop productivity ,Adaptation, Physiological ,Models, Biological ,Physiological responses ,03 medical and health sciences ,030104 developmental biology ,Cold stress ,Plant Physiological Phenomena ,010606 plant biology & botany ,Signal Transduction - Abstract
Contents Summary I. Introduction II. Cold stress and physiological responses in plants III. Sensing of cold signals in plants IV. Messenger molecules involved in cold signal transduction V. Cold signal transduction in plants VI. Conclusions and perspectives Acknowledgements References SUMMARY: Cold stress is a major environmental factor that seriously affects plant growth and development, and influences crop productivity. Plants have evolved a series of mechanisms that allow them to adapt to cold stress at both the physiological and molecular levels. Over the past two decades, much progress has been made in identifying crucial components involved in cold-stress tolerance and dissecting their regulatory mechanisms. In this review, we summarize recent major advances in our understanding of cold signalling and put forward open questions in the field of plant cold-stress responses. Answering these questions should help elucidate the molecular mechanisms underlying plant tolerance to cold stress.
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- 2018
17. The cbfs triple mutants reveal the essential functions of <scp>CBF</scp> s in cold acclimation and allow the definition of <scp>CBF</scp> regulons in Arabidopsis
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Shuhua Yang, Zhizhong Gong, Yanglin Ding, Yiting Shi, Yuxin Jia, and Xiaoyan Zhang
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0106 biological sciences ,0301 basic medicine ,Genetics ,Physiology ,Mutant ,Plant Science ,Biology ,biology.organism_classification ,01 natural sciences ,Fight-or-flight response ,03 medical and health sciences ,030104 developmental biology ,Regulon ,Arabidopsis ,Cold acclimation ,CRISPR ,Transcription factor ,Gene ,010606 plant biology & botany - Abstract
Summary In Arabidopsis, the C-repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild-type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs > cbf1,3 > cbf3. RNA-Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBFs. Our study reveals the essential functions of CBFs in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF-dependent cold signaling.
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- 2016
18. PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15
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Yiting Shi, Jianmin Zhou, Jian Hua, Shuhua Yang, Zhuoyang Li, Zhizhong Gong, Yanglin Ding, Jinlong Wang, and Xi Wang
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Ubiquitin-Protein Ligases ,Arabidopsis ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Ubiquitin ,Gene Expression Regulation, Plant ,Cold acclimation ,Arabidopsis thaliana ,Threonine ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,biology ,Arabidopsis Proteins ,Cell Biology ,biology.organism_classification ,Cell biology ,Cold Temperature ,biology.protein ,Phosphorylation ,Signal transduction ,030217 neurology & neurosurgery ,Homeostasis ,Signal Transduction ,Transcription Factors ,Developmental Biology - Abstract
Cold stress adversely affects plant growth, development, and crop productivity and quality. Plants employ cold acclimation strategy to protect them from cold damage. The transcription-factor-CBF-dependent cold signaling pathway plays a key role in plant cold acclimation. However, how this signaling pathway is dynamically and precisely regulated remains unknown. Here, we report that two U-box type E3 ubiquitin ligases, PUB25 and PUB26, positively regulate freezing tolerance in Arabidopsis thaliana. Both PUB25 and PUB26 poly-ubiquitinate MYB15, a transcriptional repressor of the CBF-dependent cold signaling pathway, leading to MYB15 degradation and thus enhanced CBF expression under cold stress. Furthermore, cold-activated OST1 specifically phosphorylates PUB25 and PUB26 at conserved threonine residues, enhancing their E3 activity and facilitating the cold-induced degradation of MYB15. Our results thus unravel the regulatory role of the OST1-PUB25/26 module in regulating the duration and amplitude of the cold response by controlling the homeostasis of the negative regulator MYB15.
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- 2019
19. <scp>OST</scp> 1‐mediated <scp>BTF</scp> 3L phosphorylation positively regulates <scp>CBF</scp> s during plant cold responses
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Shuhua Yang, Yiting Shi, Yuxin Jia, Xiaoyan Zhang, Chun-Peng Song, Zhizhong Gong, and Yanglin Ding
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0301 basic medicine ,Plant growth ,Arabidopsis ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Stress, Physiological ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Gene ,Transcription factor ,Freezing tolerance ,General Immunology and Microbiology ,Arabidopsis Proteins ,General Neuroscience ,Articles ,biology.organism_classification ,Cell biology ,Cold Temperature ,030104 developmental biology ,Protein Kinases ,Function (biology) ,Transcription Factors - Abstract
Cold stress is a major environmental factor that negatively affects plant growth and survival. OST1 has been identified as a key protein kinase in plant response to cold stress; however, little is known about the underlying molecular mechanism. In this study, we identified BTF3 and BTF3L (BTF3‐like), β‐subunits of a nascent polypeptide‐associated complex (NAC), as OST1 substrates that positively regulate freezing tolerance. OST1 phosphorylates BTF3 and BTF3L in vitro and in vivo , and facilitates their interaction with C‐repeat‐binding factors (CBFs) to promote CBF stability under cold stress. The phosphorylation of BTF3L at the Ser50 residue by OST1 is required for its function in regulating freezing tolerance. In addition, BTF3 and BTF3L proteins positively regulate the expression of CBF genes. These findings unravel a molecular mechanism by which OST1‐BTF3‐CBF module regulates plant response to cold stress.
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- 2018
20. EAR1 Negatively Regulates ABA Signaling by Enhancing 2C Protein Phosphatase Activity
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Zhizhong Gong, Yanglin Ding, Chun-Peng Song, Yu Wang, Xiaoji Wang, Ting Wu, Lingyao Kong, Shuhua Yang, Xiaofeng Zhou, Jigang Li, Kai Wang, Yang Zhao, Baoshan Wang, Junna He, and Jian-Kang Zhu
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0106 biological sciences ,0301 basic medicine ,Protein domain ,Phosphatase ,Arabidopsis ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Plant Growth Regulators ,Protein Domains ,Phosphoprotein Phosphatases ,Phosphorylation ,Enhancer ,Abscisic acid ,Research Articles ,Kinase ,Arabidopsis Proteins ,organic chemicals ,fungi ,Intracellular Signaling Peptides and Proteins ,food and beverages ,Cell Biology ,ABI1 ,Cell biology ,030104 developmental biology ,Phenotype ,chemistry ,Mutation ,Proteolysis ,Signal transduction ,010606 plant biology & botany ,Abscisic Acid ,Signal Transduction - Abstract
The reversible phosphorylation of proteins by kinases and phosphatases is an antagonistic process that modulates many cellular functions. Protein phosphatases are usually negatively regulated by inhibitor proteins. During abscisic acid (ABA) signaling, these inhibitor proteins comprise PYR1/PYL/RCAR ABA receptors, which inhibit the core negative regulators, the clade A type 2C protein phosphatases (PP2Cs). However, it is not known whether these PP2Cs are positively regulated by other proteins. Here, we identified an Arabidopsis thaliana ear1 (enhancer of aba co-receptor1) mutant that exhibits pleiotropic ABA-hypersensitive phenotypes. EAR1 encodes an uncharacterized protein that is conserved in both monocots and dicots. EAR1 interacts with the N-terminal inhibition domains of all six PP2Cs, ABA INSENSITIVE1 (ABI1), ABI2, HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-HYPERSENSITIVE GERMINATION1 (AHG1), and AHG3, during ABA signaling and enhances the activity of PP2Cs both in vitro and in vivo. ABA treatment caused EAR1 to accumulate in the nucleus. These results indicate that EAR1 is a negative regulator of ABA signaling that enhances the activity of PP2Cs by interacting with and releasing the N-terminal autoinhibition of these proteins.
- Published
- 2017
21. OST1 Kinase Modulates Freezing Tolerance by Enhancing ICE1 Stability in Arabidopsis
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Qi Xie, Zhizhong Gong, Yanglin Ding, Hui Li, Shuhua Yang, and Xiaoyan Zhang
- Subjects
Arabidopsis ,Biology ,Genes, Plant ,General Biochemistry, Genetics and Molecular Biology ,Gene Expression Regulation, Plant ,Freezing ,Protein kinase A ,Molecular Biology ,Transcription factor ,Regulation of gene expression ,Arabidopsis Proteins ,Kinase ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,Ubiquitin ligase ,Biochemistry ,biology.protein ,Phosphorylation ,Signal transduction ,Protein Kinases ,Signal Transduction ,Transcription Factors ,Developmental Biology - Abstract
SummaryCold stress is a major environmental factor that limits plant growth and development. The C-repeat-binding factor (CBF)-dependent cold signaling pathway is extensively studied in Arabidopsis; however, the specific protein kinases involved in this pathway remain elusive. Here we report that OST1 (OPEN STOMATA 1), a well-known Ser/Thr protein kinase in ABA signaling, acts upstream of CBFs to positively regulate freezing tolerance. The ost1 mutants show freezing hypersensitivity, whereas transgenic plants overexpressing OST1 exhibit enhanced freezing tolerance. The OST1 kinase is activated by cold stress. Moreover, OST1 interacts with both the transcription factor ICE1 and the E3 ligase HOS1 in the CBF pathway. Cold-activated OST1 phosphorylates ICE1 and enhances its stability and transcriptional activity. Meanwhile, OST1 interferes with the interaction between HOS1 and ICE1, thus suppressing HOS1-mediated ICE1 degradation under cold stress. Our results thus uncover the unexpected roles of OST1 in modulating CBF-dependent cold signaling in Arabidopsis.
- Published
- 2015
22. Natural variation in CTB4a enhances rice adaptation to cold habitats
- Author
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Hongliang Zhang, Shiquan Shen, Haifeng Guo, Shuhua Yang, Lei Zhou, Zhanying Zhang, Jianping Yu, Luyuan Dai, Liang Ma, Yanglin Ding, Weiwei Zheng, Yawen Zeng, Gangling Li, Zichao Li, Jinjie Li, Jilong Li, Hong-li Shi, Xingming Sun, Yan Guo, Shuming Yang, and Yinghua Pan
- Subjects
0106 biological sciences ,0301 basic medicine ,Science ,Plant genetics ,Quantitative Trait Loci ,General Physics and Astronomy ,Quantitative trait locus ,Genes, Plant ,01 natural sciences ,Acclimatization ,General Biochemistry, Genetics and Molecular Biology ,Japonica ,Article ,Chromosomes, Plant ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Genetic variation ,Botany ,Promoter Regions, Genetic ,Gene ,Ecosystem ,Phylogeny ,Multidisciplinary ,biology ,ATP synthase ,food and beverages ,Chromosome Mapping ,Genetic Variation ,Oryza ,General Chemistry ,biology.organism_classification ,Adaptation, Physiological ,Cold Temperature ,030104 developmental biology ,Haplotypes ,biology.protein ,Adaptation ,010606 plant biology & botany - Abstract
Low temperature is a major factor limiting rice productivity and geographical distribution. Improved cold tolerance and expanded cultivation to high-altitude or high-latitude regions would help meet growing rice demand. Here we explored a QTL for cold tolerance and cloned the gene, CTB4a (cold tolerance at booting stage), encoding a conserved leucine-rich repeat receptor-like kinase. We show that different CTB4a alleles confer distinct levels of cold tolerance and selection for variation in the CTB4a promoter region has occurred on the basis of environmental temperature. The newly generated cold-tolerant haplotype Tej-Hap-KMXBG was retained by artificial selection during temperate japonica evolution in cold habitats for low-temperature acclimation. Moreover, CTB4a interacts with AtpB, a beta subunit of ATP synthase. Upregulation of CTB4a correlates with increased ATP synthase activity, ATP content, enhanced seed setting and improved yield under cold stress conditions. These findings suggest strategies to improve cold tolerance in crop plants., Low temperature is a major factor limiting productivity in rice. Here the authors show that the CTB4a gene confers cold tolerance to japonica varieties adapted to cold habitats at the booting stage of development, and propose that CTB4a acts via an interaction with the beta subunit of ATP synthase.
- Published
- 2017
23. BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis
- Author
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Shuhua Yang, Keyi Ye, Hui Li, Chun-Peng Song, Yiting Shi, Zhizhong Gong, and Yanglin Ding
- Subjects
0106 biological sciences ,0301 basic medicine ,Arabidopsis ,Plant Science ,Biology ,Genes, Plant ,01 natural sciences ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Brassinosteroids ,Freezing ,Cold acclimation ,Arabidopsis thaliana ,Phosphorylation ,Kinase activity ,Transcription factor ,Research Articles ,Arabidopsis Proteins ,Cold-Shock Response ,Intracellular Signaling Peptides and Proteins ,Nuclear Proteins ,Cell Biology ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,Ubiquitin ligase ,Cold shock response ,030104 developmental biology ,biology.protein ,Signal transduction ,Protein Kinases ,Signal Transduction ,Transcription Factors ,010606 plant biology & botany - Abstract
Cold acclimation is a crucial strategy for plant survival at freezing temperatures. C-REPEAT BINDING FACTOR (CBF) genes are rapidly and transiently induced by low temperature and play important roles in cold acclimation. However, the mechanism underlying the attenuation of CBF expression during the later stages of the cold stress response is obscure. Here, we show that the protein kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2) interacts with and phosphorylates INDUCER OF CBF EXPRESSION1 (ICE1) in Arabidopsis (Arabidopsis thaliana) under prolonged cold stress, facilitating the interaction between ICE1 and the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 and thereby promoting ICE1 degradation. The kinase activity of BIN2 is inhibited during the early stages of the cold stress response and is subsequently restored, suggesting that BIN2 mainly downregulates ICE1 abundance when CBF expression is attenuated. A loss-of-function mutation of ICE1 partially suppresses the cold-induced expression of CBFs and compromises the enhanced freezing tolerance of bin2-3 bil1 bil2. These findings reveal an important role for BIN2 in fine-tuning CBF expression, and thus in balancing plant growth and the cold stress response.
- Published
- 2019
24. ESCRT-I Component VPS23A Affects ABA Signaling by Recognizing ABA Receptors for Endosomal Degradation
- Author
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Pedro L. Rodriguez, Feifei Yu, Qi Xie, Yanglin Ding, Yaorong Wu, Qingliang Li, Xiaoqiang Cao, Miaomiao Tian, Borja Belda-Palazón, Lijuan Lou, and Shuhua Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,ESCRTs ,Endosome ,Arabidopsis ,Receptors, Cell Surface ,Plant Science ,ABA receptors ,Bioinformatics ,01 natural sciences ,ESCRT ,03 medical and health sciences ,chemistry.chemical_compound ,Downregulation and upregulation ,Ubiquitin ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,Receptor ,Molecular Biology ,Abscisic acid ,Pyr1 ,biology ,Endosomal Sorting Complexes Required for Transport ,Arabidopsis Proteins ,fungi ,food and beverages ,biology.organism_classification ,Cell biology ,Endosomal trafficking ,030104 developmental biology ,chemistry ,biology.protein ,Plant hormone ,010606 plant biology & botany ,Abscisic Acid ,Protein Binding ,Signal Transduction - Abstract
[EN] Recent discovery of PYR/PYL/RCAR-type abscisic acid (ABA) receptors has become one of most significant advances in plant science in the past decade. In mammals, endosomal sorting acts as an important pathway to downregulate different types of receptors, but its role in plant hormone signaling is poorly understood. Here, we report that an ubiquitin E2-like protein, VPS23A, which is a key component of ESCRT-I, negatively regulates ABA signaling. VPS23A has epistatic relationship with PYR/PYL/RCAR-type ABA receptors and disruption of VPS23A enhanced the activity of key kinase OST1 in the ABA signaling pathway under ABA treatment. Moreover, VPS23A interacts with PYR1/PYLs and K63-linked diubiquitin, and PYL4 possesses K63-linked ubiquitinated modification in vivo. Further analysis revealed that VPS23A affects the subcellular localization of PYR1 and the stability of PYL4. Taken together, our results suggest that VPS23A affects PYR1/PYL4 via vacuole-mediated degradation, providing an advanced understanding of both the turnover of ABA receptors and ESCRTs in plant hormone signaling., This research was supported by grant 2016YFA0500500 from the National Basic Research Program of China and NSFC 31571441 from the National Science Foundation of China.
- Published
- 2016
25. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis
- Author
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Yuxin, Jia, Yanglin, Ding, Yiting, Shi, Xiaoyan, Zhang, Zhizhong, Gong, and Shuhua, Yang
- Subjects
Cold Temperature ,Base Sequence ,Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Sequence Analysis, RNA ,Stress, Physiological ,Acclimatization ,Freezing ,Mutation ,Arabidopsis ,Genes, Plant ,Transcriptome ,Regulon - Abstract
In Arabidopsis, the C-repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild-type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs cbf1,3 cbf3. RNA-Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBFs. Our study reveals the essential functions of CBFs in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF-dependent cold signaling.
- Published
- 2016
26. Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3
- Author
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Yuancong Wang, Yao Zhang, Shanshan Wang, Yule Liu, Yanglin Ding, Jingyan Liu, Xiaoyan Zhang, and Shuhua Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Physiology ,Mutant ,Arabidopsis ,Down-Regulation ,Autoimmunity ,Plant Science ,Plasma protein binding ,medicine.disease_cause ,Genes, Plant ,01 natural sciences ,Models, Biological ,03 medical and health sciences ,Suppression, Genetic ,Downregulation and upregulation ,Gene Expression Regulation, Plant ,Loss of Function Mutation ,medicine ,Amino Acid Sequence ,Binding site ,Cloning, Molecular ,Receptors, Immunologic ,Genes, Suppressor ,Gene ,Genetics ,Mutation ,biology ,Base Sequence ,Arabidopsis Proteins ,Temperature ,biology.organism_classification ,Phenotype ,030104 developmental biology ,Gain of Function Mutation ,010606 plant biology & botany ,Protein Binding ,Subcellular Fractions - Abstract
Toll/interleukin receptor (TIR)-nucleotide binding site (NB)-type (TN) proteins are encoded by a family of 21 genes in the Arabidopsis genome. Previous studies have shown that a mutation in the TN gene CHS1 activates the activation of defense responses at low temperatures. However, the underlying molecular mechanism remains unknown. To genetically dissect chs1-mediated signaling, we isolated genetic suppressors of chs1-2 (soc). Several independent soc mutants carried mutations in the same TIR-NB-leucine-rich repeat (LRR) (TNL)-encoding gene SOC3, which is adjacent to CHS1 on chromosome 1. Expression of SOC3 was upregulated in the chs1-2 mutant. Mutations in six soc3 alleles and downregulation of SOC3 by an artificial microRNA construct fully rescued the chilling sensitivity and defense defects of chs1-2. Biochemical studies showed that CHS1 interacted with the NB and LRR domains of SOC3; however, mutated chs1 interacted with the TIR, NB and LRR domains of SOC3 in vitro and in vivo. This study reveals that the TN protein CHS1 interacts with the TNL protein SOC3 to modulate temperature-dependent autoimmunity.
- Published
- 2016
27. Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases
- Author
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Yan Guo, Qi Xie, Shuhua Yang, Jigang Li, Yujuan Zhu, Lingyao Kong, Zhizhong Gong, Yanglin Ding, Jingjing Meng, Zhizhong Chen, and Jinkui Cheng
- Subjects
Proteasome Endopeptidase Complex ,Ubiquitin-Protein Ligases ,Mutant ,Phosphatase ,Arabidopsis ,General Physics and Astronomy ,Article ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Phosphoprotein Phosphatases ,Abscisic acid ,Multidisciplinary ,biology ,Arabidopsis Proteins ,organic chemicals ,fungi ,Wild type ,food and beverages ,General Chemistry ,biology.organism_classification ,ABI1 ,Biochemistry ,chemistry ,Mutation ,Signal transduction ,Abscisic Acid ,Signal Transduction - Abstract
Clade A protein phosphatase 2Cs (PP2Cs) are abscisic acid (ABA) co-receptors that block ABA signalling by inhibiting the downstream protein kinases. ABA signalling is activated after PP2Cs are inhibited by ABA-bound PYR/PYL/RCAR ABA receptors (PYLs) in Arabidopsis. However, whether these PP2Cs are regulated by other factors remains unknown. Here, we report that ABI1 (ABA-INSENSITIVE 1) can interact with the U-box E3 ligases PUB12 and PUB13, but is ubiquitinated only when it interacts with ABA receptors in an in vitro assay. A mutant form of ABI1-1 that is unable to interact with PYLs is more stable than the wild-type protein. Both ABI1 degradation and all tested ABA responses are reduced in pub12 pub13 mutants compared with the wild type. Introducing the abi1-3 loss-of-function mutation into pub12 pub13 mutant recovers the ABA-insensitive phenotypes of the pub12 pub13 mutant. We thus uncover an important regulatory mechanism for regulating ABI1 levels by PUB12 and PUB13., Signaling by the plant hormone abscisic acid (ABA) is regulated by the ABI1 protein phosphatase. Here Kong et al. propose that ABA signaling is fine-tuned by ubiquitination of ABI1 which promotes ABI degradation in response to ABA.
- Published
- 2015
28. MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis
- Author
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Zhizhong Gong, Yanglin Ding, Shuhua Yang, Shuqun Zhang, Yiting Shi, Hui Li, and Xiaoyan Zhang
- Subjects
0106 biological sciences ,0301 basic medicine ,Mutant ,Cell Biology ,Biology ,biology.organism_classification ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Biochemistry ,Arabidopsis ,Phosphorylation ,Inducer ,Protein kinase A ,Molecular Biology ,Gene ,Transcription factor ,Function (biology) ,010606 plant biology & botany ,Developmental Biology - Abstract
Summary Low temperatures affect plant growth, development, productivity, and ecological distribution. Expression of the C-repeat-binding factor ( CBF ) transcription factors is induced by cold stress, which in turn activates downstream cold-responsive ( COR ) genes that are required for the acquisition of freezing tolerance. Inducer of CBF expression 1 (ICE1) is a master regulator of CBF s, and ICE1 stability is crucial for its function. However, the regulation of ICE1 is not well understood. Here, we report that mitogen-activated protein kinase 3 (MPK3) and MPK6 interact with and phosphorylate ICE1, which reduces its stability and transcriptional activity. Consistently, the mpk3 and mpk6 single mutants and the mpk3 mpk6 double mutants show enhanced freezing tolerance, whereas MPK3/MPK6 activation attenuates freezing tolerance. Phosphor-inactive mutations of ICE1 complement freezing sensitivity in the ice1-2 mutant. These combined results indicate that MPK3/MPK6 phosphorylate and destabilize ICE1, which negatively regulates CBF expression and freezing tolerance in plants.
- Published
- 2017
29. Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response
- Author
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Zhizhong Gong, Yanglin Ding, Yan Guo, Yiting Shi, Ziyan Liu, Zhen Li, Yuxin Jia, and Shuhua Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Proteasome Endopeptidase Complex ,Time Factors ,Genotype ,Membrane Fluidity ,Mutant ,Active Transport, Cell Nucleus ,Arabidopsis ,Protein Serine-Threonine Kinases ,Biology ,01 natural sciences ,03 medical and health sciences ,medicine ,Thermosensing ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Cell Nucleus ,Arabidopsis Proteins ,Protein Stability ,Cold-Shock Response ,Cell Membrane ,Cell Biology ,Plants, Genetically Modified ,Plant cell ,Adaptation, Physiological ,Cell biology ,Cold Temperature ,Enzyme Activation ,Cytosol ,Phenotype ,030104 developmental biology ,medicine.anatomical_structure ,Membrane ,14-3-3 Proteins ,Mutation ,Proteolysis ,Nuclear transport ,Protein Kinases ,Nucleus ,Signal Transduction ,Transcription Factors ,010606 plant biology & botany - Abstract
In plant cells, changes in fluidity of the plasma membrane may serve as the primary sensor of cold stress; however, the precise mechanism and how the cell transduces and fine-tunes cold signals remain elusive. Here we show that the cold-activated plasma membrane protein cold-responsive protein kinase 1 (CRPK1) phosphorylates 14-3-3 proteins. The phosphorylated 14-3-3 proteins shuttle from the cytosol to the nucleus, where they interact with and destabilize the key cold-responsive C-repeat-binding factor (CBF) proteins. Consistent with this, the crpk1 and 14-3-3κλ mutants show enhanced freezing tolerance, and transgenic plants overexpressing 14-3-3λ show reduced freezing tolerance. Further study shows that CRPK1 is essential for the nuclear translocation of 14-3-3 proteins and for 14-3-3 function in freezing tolerance. Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF stability, which ensures a faithfully adjusted response to cold stress of plants.
- Published
- 2017
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