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Aroma is one of the most important factors affecting the quality of citrus fruit and its processed products. We use electronic nose (E-nose) to detect and analyze volatile components in citrus. An E-nose is an artificial intelligence system with strong independence and fast detection speed. It combines an array of gas sensors and intelligent algorithms designed to analyze gas. Moreover, it has the ability to detect and analyze volatile components. Feature extraction is the first step of sensor signal processing and plays an important role in subsequent pattern recognition. Cellular neural network (CNN) is a real-time high-speed parallel array processor and a locally connected network, which has mature applications in the field of image processing. Previous researches have shown that CNN has an outstanding impact on image feature extractio. In this paper, the traditional CNN is improved and a template for dynamic feature extraction of the E-nose response curve is proposed. In addition, we provide users with single-template and multi-template solutions which can be applied in different environments. To free up the computational power of occupancy, the effect of the single-template version of CNN is not as effective as the multi-template version, but it still has good feature extraction ability. These two solutions prove that CNN is sensitive to dynamic features. In order to make the results more representative, we choose several traditional feature extraction methods for comparison.

The abnormal expression of RNA binding proteins (RBPs) in tumors can regulate the functions of immune genes and affect immune microenvironment. The characterization of immune infiltration and its regulatory mechanism from the perspective of RBPs and RBP-regulated immune genes in high-grade gliomas were comprehensively studied by bioinformatics. Prognosis-related RBPs and associated immune genes were obtained from the cancer genome atlas database (TCGA). By clustering RBPs, the potential relationship between the different clusters of RBPs expression and immune infiltration was explored. Immune-related RBP constructs immunosuppressive microenvironment in glioma by regulating immune genes and immune-related genes. Immunosuppression was the predominant type of phenotype with poor prognosis, while immunoinflammation was the predominant type of immunoinflammatory phenotype with good prognosis. RBP can construct immunosuppressive microenvironment by regulating immunosuppressive cells and stromal activation-related factors through directly and indirectly ways. Stromal activation plays a bridging role in tumor immunosuppressive regulation. The immunophenotyp depends on different enrichment states of immune-related RBP, which can change dynamically with the change of tumor pathological grade and affect the prognosis of patients.Our study suggests glioma related RBPs can potentially establish an immunosuppressive microenvironment by regulating immune genes and immune-related genes, which can be directly affected by RBPs clusters with distinct prognosis.

Background The goal of this study is to evaluate the safety and efficacy of a novel catheter for right radial artery approach cerebral angiography. Methods Patients from the Neurology Department of The Second Affiliated Hospital of Guangxi Traditional Chinese Medical University who underwent diagnostic cerebral angiography of either the left vertebral artery dominant type or balanced type were enrolled in this study. Results A total of 167 patients were treated between February 2016 and December 2017, of whom 44 were excluded based on study exclusion criteria and 123 were enrolled in the present analysis. Bilateral subclavian artery catheterization and bilateral common carotid artery catheterization were conducted successfully in all 123 patients. The success rate of selective catheterization of the left vertebral artery was 87.8% (108/123). The success rate of selective catheterization of the right vertebral artery using the novel catheter was 89.0% (73/82). The average fluoroscopy time was 6.5 ± 3.4 min, the average operation duration was 47 ± 3.7 (range 50–90) min, and the average dosage of contrast agent was 112.3 ± 8.1 mL. One patient exhibited an absence of pulse in the punctual radial artery after the removal of the arterial compression band, but there was no evidence of ischemia of the distal hand. One patient who was undergoing dual anti-platelet drug treatment suffered from bleeding at the puncture point when deflated for 2 hr after operation; this patient was re-pressurized and re-timed. Conclusions This novel catheter improved the success rate of selective left vertebral artery catheterization, and allowed for simplification of the relevant surgical steps. The controllability of this novel catheter was satisfactory, and its associated surgical risk was found to be low.

The Hedgehog (Hh) signalling pathway is essential for cellular proliferation and differentiation during embryonic development. Gain and loss of function of Hh signalling are known to result in an array of craniofacial malformations. To determine the critical period for Hh pathway antagonist-induced frontal bone hypoplasia, we examined patterns of dysmorphology caused by Hh signalling inhibition. Pregnant mice received a single oral administration of Hh signalling inhibitor GDC-0449 at 100 mg•kg−1 or 150 mg•kg−1 body weight at preselected time points between embryonic days (E)8.5 and 12.5. The optimal teratogenic concentration of GDC-0449 was determined to be 150 mg•kg−1. Exposure between E9.5 and E10.5 induced frontal bone dysplasia, micrognathia and limb defects, with administration at E10.5 producing the most pronounced effects. This model showed decreased ossification of the frontal bone with downregulation of Hh signalling. The osteoid thickness of the frontal bone was significantly reduced. The amount of neural crest-derived frontal bone primordium was reduced after GDC-0449 exposure owing to a decreased rate of cell proliferation and increased cell death., Bone Development: Critical period for Hedgehog pathway in bone development During embryonic development, the Hedgehog signalling pathway regulates the migration, proliferation and differentiation of cranial neural crest cells in the early frontal bone. The Hedgehog signalling pathway transmits information to embryonic cells for their proper cell differentiation, and increased or reduced function of that signalling results in various craniofacial malformations. A team headed by Weihui Chen at Fujian Medical University in China investigated the patterns of abnormalities caused by inhibition of Hedgehog signalling in pregnant mice at preselected embryonic time points. The team was able to identify the critical period for sensitivity to GDC-0449, a potent Hedgehog signalling inhibitor. The authors believe that their mouse model can be effective in further investigating the mechanisms of craniofacial malformations and will have a profound impact on identifying candidate human disease genes and associated environmental factors.

As a stable environmental contaminant, nonylphenol (NP) has been shown to induce some neurological deficits in the cerebellum, although the underlying mechanism is still unknown. In the present study, we aimed to investigate the effects of perinatal exposure to NP on myelination, an important process essential for the intact cerebellar function, in the offspring cerebellum. Exposure to NP delayed the myelination in the offspring cerebellum during perinatal period. The myelination recovered in the cerebellum of offspring exposed to NP over time, and returned to normal in adulthood. In addition, perinatal exposure to NP reduced mature oligodendrocytes (myelin-forming glial cells) and increased astrocytes in the offspring cerebellum. BMP signaling is believed to negatively regulate oligodendrogliogenesis and myelination. In the present study, BMP4, p-Smad1/5, and ID4, key members of BMP signaling, were increased in the cerebellum of offspring exposed to NP. Taken together, these lines of evidence suggest that the activation of BMP signaling may underlie the decreased oligodendrogliogenesis and increased astrogliogenesis, and the consequent delay of myelination in the cerebellum of offspring perinatally exposed to NP.