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To improve the translation of preclinical cancer research data to successful clinical effect, there is an increasing focus on the use of primary patient‐derived cancer cells with limited growth in culture to reduce genetic and phenotype drift. However, these primary lines are less amenable to standardly used methods of exogenous DNA introduction. Adeno‐associated viral (AAV) vectors display tropism for a wide range of human tissues, avidly infect primary cells and have a good safety profile. In the present study, we therefore used a next‐generation sequencing (NGS) barcoded AAV screening method to assess transduction capability of a panel of 36 AAVs in primary cell lines representing high‐grade glioma (HGG) brain tumours including glioblastoma (GBM) and diffuse intrinsic pontine glioma (DIPG)/diffuse midline glioma (DMG). As proof of principle, we created a reporter construct to analyse activity of the transcriptional co‐activators yes‐associated protein (YAP) and transcriptional co‐activator with PDZ‐binding motif (TAZ). Transcriptional activation was monitored by promoter‐driven expression of the Timer fluorescent tag, a protein that fluoresces green immediately after transcription and transitions to red fluorescence over time. As expected, attempts to express the reporter in primary HGG cells from plasmid expression vectors were unsuccessful. Using the top candidate from the AAV screen, we demonstrate successful AAV‐mediated transduction of HGG cells with the YAP/TAZ dynamic activity reporter. In summary, the NGS‐screening approach facilitated screening of many potential AAVs, identifying vectors that can be used to study the biology of primary HGG cells.

Abstract Background Influenza outbreaks have occurred frequently these years, especially in the summer of 2022 when the number of influenza cases in southern provinces of China increased abnormally. However, the exact evidence of the driving factors involved in the prodrome period is unclear, posing great difficulties for early and accurate prediction in practical work. Methods In order to avoid the serious interference of strict prevention and control measures on the analysis of influenza influencing factors during the COVID-19 epidemic period, only the impact of meteorological and air quality factors on influenza A (H3N2) in Xiamen during the non coronavirus disease 2019 (COVID-19) period (2013/01/01-202/01/24) was analyzed using the distribution lag non-linear model. Phylogenetic analysis of influenza A (H3N2) during 2013–2022 was also performed. Influenza A (H3N2) was predicted through a random forest and long short-term memory (RF-LSTM) model via actual and forecasted meteorological and influenza A (H3N2) values. Results Twenty nine thousand four hundred thirty five influenza cases were reported in 2022, accounting for 58.54% of the total cases during 2013–2022. A (H3N2) dominated the 2022 summer epidemic season, accounting for 95.60%. The influenza cases in the summer of 2022 accounted for 83.72% of the year and 49.02% of all influenza reported from 2013 to 2022. Among them, the A (H3N2) cases in the summer of 2022 accounted for 83.90% of all A (H3N2) reported from 2013 to 2022. Daily precipitation(20–50 mm), relative humidity (70–78%), low (≤ 3 h) and high (≥ 7 h) sunshine duration, air temperature (≤ 21 °C) and O3 concentration (≤ 30 µg/m3, > 85 µg/m3) had significant cumulative effects on influenza A (H3N2) during the non-COVID-19 period. The daily values of PRE, RHU, SSD, and TEM in the prodrome period of the abnormal influenza A (H3N2) epidemic (19–22 weeks) in the summer of 2022 were significantly different from the average values of the same period from 2013 to 2019 (P

ObjectiveTo use bibliometric methods to analyze the prospects and development trends of artificial intelligence(AI) in oncology nursing from 1994 to 2024, providing guidance and reference for oncology nursing professionals and researchers.MethodsThe core set of the Web of Science database was searched for articles from 1994 to 2024. The R package “Bibliometrix” was used to analyze the main bibliometric features, creating a three-domain chart to display relationships among institutions, countries, and keywords. VOSviewer facilitated co-authorship analysis and its visualization was used for co- occurrence analysis. CiteSpace calculated citation bursts and keyword occurrences.ResultsA total of 517 articles were retrieved, representing 80 countries/regions. The United States had the highest number of publications, with 188 articles (36.4%), followed by China with 79 articles (15.3%). The top 10 institutions in terms of publication output were all U.S.-based universities or cancer research institutes, with Harvard University ranking first. Prominent research teams, such as those led by Repici, Aerts, and Almangush, have made significant contributions to studies on AI in tumor risk factor identification and symptom management. In recent years, the keywords with the highest burst strength were “model” and “human papillomavirus.” The most studied tumor type was breast cancer. While Cancers published the highest number of articles, journals such as CA: A Cancer Journal for Clinicians and PLOS ONE had higher impact and citation rates.ConclusionBy analyzing the volume of AI literature in oncology nursing, combined with the statistical analysis of institutions, core authors, journals, and keywords, the research hotspots and trends in the application of AI in oncology nursing over the past 30 years are revealed. AI in oncology nursing is entering a stage of rapid development, providing valuable reference for scholars and professionals in the field.

Abstract Objective At different times, public health faces various challenges and the degree of intervention measures varies. The research on the impact and prediction of meteorology factors on influenza is increasing gradually, however, there is currently no evidence on whether its research results are affected by different periods. This study aims to provide limited evidence to reveal this issue. Methods Daily data on influencing factors and influenza in Xiamen were divided into three parts: overall period (phase AB), non-COVID-19 epidemic period (phase A), and COVID-19 epidemic period (phase B). The association between influencing factors and influenza was analysed using generalized additive models (GAMs). The excess risk (ER) was used to represent the percentage change in influenza as the interquartile interval (IQR) of meteorology factors increases. The 7-day average daily influenza cases were predicted using the combination of bi-directional long short memory (Bi-LSTM) and random forest (RF) through multi-step rolling input of the daily multifactor values of the previous 7-day. Results In periods A and AB, air temperature below 22 °C was a risk factor for influenza. However, in phase B, temperature showed a U-shaped effect on it. Relative humidity had a more significant cumulative effect on influenza in phase AB than in phase A (peak: accumulate 14d, AB: ER = 281.54, 95% CI = 245.47 ~ 321.37; A: ER = 120.48, 95% CI = 100.37 ~ 142.60). Compared to other age groups, children aged 4–12 were more affected by pressure, precipitation, sunshine, and day light, while those aged ≥ 13 were more affected by the accumulation of humidity over multiple days. The accuracy of predicting influenza was highest in phase A and lowest in phase B. Conclusions The varying degrees of intervention measures adopted during different phases led to significant differences in the impact of meteorology factors on influenza and in the influenza prediction. In association studies of respiratory infectious diseases, especially influenza, and environmental factors, it is advisable to exclude periods with more external interventions to reduce interference with environmental factors and influenza related research, or to refine the model to accommodate the alterations brought about by intervention measures. In addition, the RF-Bi-LSTM model has good predictive performance for influenza.

Abstract Background Assisted reproductive technology (ART) is the most effective method to treat infertility and the pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) is a challenging filed in infertility. Microbes in the female reproductive tract are considered to be associated with gynecological and obstetric diseases. However, its effects on embryo implantation failure are unsured. Purpose This study aimed to investigate reproductive tract dysbiosis, identify different bacteria in reproductive tract as potential biomarkers of embryo implantation failure and demonstrate the pathogenesis through metabolites analysis. Methods We compared the data from 16S rRNA gene and metagenome in reproductive tracts through QIIME2 and HUMAnN2 by the times of embryo implantation failure on 239 infertile patients and 17 healthy women. Results Our study revealed a strong positive correlation between Lactobacillus abundance and embryo implantation success (IS) after IVF-ET. The microbial community composition and structure in reproductive tract showed substantially difference between the embryo implantation failure (IF) and healthy control. Moreover, we established a diagnostic model through receiver operating characteristic (ROC) with 0.913 area under curve (AUC) in IS and multiple implantation failures (MIF), verified its effectiveness with an AUC = 0.784 demonstrating microbial community alterations could efficiently discriminate MIF patients. Metagenome functional analyses of vaginal samples from another independent infertile patients after IVF-ET revealed the L-lysine synthesis pathway enriched in IF patients, along with ascended vaginal pH and decreased Lactobacillus abundance. Conclusions This study clarifies several independent relationships of bacteria in vagina and endometrial fluid on embryo implantation failure and undoubtedly broadens the understanding about female reproductive health.

Introduction: Cervical disc herniation (CDH) is a common condition, usually caused by excessive strain or trauma to the spine. Initially, it is treated conservatively; however, complex and resistant cases may require a surgical intervention. Aim: We aimed to compare the clinical effect of endoscopy-assisted anterior cervical discectomy and fusion (ACDF) with internal fixation and conventional surgery in the treatment of CDH. Materials and methods: Patients with CDH who underwent ACDF with fixation at the Zhoushan Dinghai Guanghua Hospital were enrolled. Of them, 10 individuals were treated with conventional ACDF (conventional surgery group), and the other 10 with endoscopy-assisted ACDF (endoscopy-assisted surgery group). The general characteristics, postoperative Japanese Orthopedic Association (JOA), visual analogue scale (VAS), 12-Item Short Form Survey Physical Component Summary (SF-12 PCS), and SF-12 Mental Component Summary (SF-12 MCS) scores, physiological stress response, rate of the improved JOA score (RIS), hemoglobin level, and bone graft fusion were compared between the groups. Results: Outcomes of the patients treated with endoscopy-assisted surgery were clearly superior to those observed in the conventional surgery group. The postoperative JOA, VAS, SF-12 PCS, and SF-12 MCS scores and RIS in the endoscopy-assisted surgery group were higher than in the conventional surgery group (P

The diseases of the central nervous system (CNS) often cause irreversible damage to the human body and have a poor prognosis, posing a significant threat to human health. They have brought enormous burdens to society and healthcare systems. However, due to the complexity of their causes and mechanisms, effective treatment methods are still lacking. Vagus nerve stimulation (VNS), as a physical therapy, has been utilized in the treatment of various diseases. VNS has shown promising outcomes in some CNS diseases and has been approved by the Food and Drug Administration (FDA) in the United States for epilepsy and depression. Moreover, it has demonstrated significant potential in the treatment of stroke, consciousness disorders, and Alzheimer’s disease. Nevertheless, the exact efficacy of VNS, its beneficiaries, and its mechanisms of action remain unclear. This article discusses the current clinical evidence supporting the efficacy of VNS in CNS diseases, providing updates on the progress, potential, and potential mechanisms of action of VNS in producing effects on CNS diseases.

It’s well acknowledged that noise exposure has become a major environmental risk factor of public health. The previous standpoint holds that the main harm of noise exposure is to cause hearing loss of human. However, in the past two decades a large number of studies have linked the noise exposure to various cerebral changes. In this review, we summarized that noise exposure led to cerebral changes through breaking the redox balance, inducing neuroinflammation and neuronal apoptosis and altering the neurotransmission in numerous brain areas, including cortex, thalamus, hippocampus, amygdala, striatum and cerebellum. Those cerebral changes finally result in a variety of disorders, such as tinnitus, anxiety, depression, cognitive impairment and motor dysfunction. Furthermore, we reviewed several antioxidants, such as resveratrol, vitamin C, curcumin, N-acetylcysteine and α-asarone, and highlighted their protective mechanisms against noise exposure, aiming to provide a promising strategy to prevent and treat noise exposure-induced diseases. Taken together, noise exposure induces various cerebral changes and further leads to disorders in the central nervous system, which can be ameliorated by the treatment with antioxidants.

Abstract LY86, also known as MD1, has been implicated in various pathophysiological processes including inflammation, obesity, insulin resistance, and immunoregulation. However, the role of LY86 in cholesterol metabolism remains incompletely understood. Several studies have reported significant up-regulation of LY86 mRNA in atherosclerosis; nevertheless, the regulatory mechanism by which LY86 is involved in this disease remains unclear. In this study, we aimed to investigate whether LY86 affects ox-LDL-induced lipid accumulation in macrophages. Firstly, we confirmed that LY86 is indeed involved in the process of atherosclerosis and found high expression levels of LY86 in human atherosclerotic plaque tissue. Furthermore, our findings suggest that LY86 may mediate intracellular lipid accumulation induced by ox-LDL through the SREBP2/HMGCR pathway. This mechanism could be associated with increased cholesterol synthesis resulting from enhanced endoplasmic reticulum stress response.

Abstract Currently, there is still a lack of novel and effective drug targets to improve the prognosis of hepatocellular carcinoma (HCC). Additionally, the role of CHEK2 in HCC has not been reported yet. The eQTLgen database and two HCC Genome-Wide Association Study (GWAS) datasets (ieu-b-4953, ICD10 C22.0) were used to find the drug target: CHEK2. Next, Colony, Edu, β-gal, and cell cycle analysis were facilitated to evaluate the role of CHEK2 knockout in HCC. In addition, Nultin-3 was added to evaluate the apoptosis of TP53-mutated HCC cells with CHEK2 knockout. Furthermore, MitoSox, electron microscopy, mitochondrial ATP, and NADH+/NADH levels were assessed in the CHEK2 knockout HCC cells with or without Metformin. Finally, cell-derived tumor xenograft was used to evaluate the role of CHEK2 knockout in vivo. We initially identified a potential drug target, CHEK2, through GWAS data analysis. Furthermore, we observed a significant upregulation of CHEK2 expression in HCC, which was found to be correlated with a poor prognosis. Subsequently, the results indicated that knocking out CHEK2 selectively affects the proliferation, cell cycle, senescence, and apoptosis of TP53-mutant HCC cells. Additionally, the introduction of Nultin-3 further intensified the functional impact on TP53-mutant cells. Then ClusterProfiler results showed high CHEK2 and TP53 mutation group was positively enriched in the mitochondrial ATP pathway. Then we used MitoSox, electron microscopy, mitochondrial ATP, and NADH + /NADH assay and found knockout of CHECK could induce the ATP pathway to inhibit the growth of HCC. Our research introduces a novel drug target for TP53-mutant HCC cells via mitochondrial ATP, addressing the limitation of Nultin-3 as a standalone treatment that does not induce tumor cell death.

Age-related hearing loss (ARHL) is the most common sensory disorder associated with human aging. Chronic inflammation is supposed to be an important contributor to ARHL. Yet, the underlying mechanisms of developing cochlear inflammation are still not well understood. In this study, we found that the inflammation, endoplasmic reticulum (ER) stress and necroptosis signalings are activated in the cochlea of aged C57BL/6 mice. ER stress activator tunicamycin (TM) induced necroptosis in cochlear HEI-OC1 cells and cochlear explants, while necroptosis inhibitors protected cochlear cells from ER stress-induced cell death. The antioxidants inhibited necroptosis and protected HEI-OC1 cells from TM insults. Necroptotic HEI-OC1 cells promoted the activation of the co-cultured macrophages via Myd88 signaling. Moreover, necroptosis inhibitor protected from TM-induced hearing loss, and inhibited inflammation in C57BL/6 mice. These findings suggest that ER stress-induced necroptosis promotes cochlear inflammation and hearing loss. Targeting necroptosis serves as a potential approach for the treatment of cochlear inflammation and ARHL.

The concept of minimally invasive liver surgery has been widely accepted due to the advantages of fast recovery. Robotic hepatectomy is different from laparoscopic hepatectomy due to its fixed joint instruments, operation mode and magnification. This manuscript aims to compare the safety and efficacy of robotic and laparoscopic hepatectomy in the treatment of liver diseases. In this manuscript, we retrospectively studied 246 patients (143 with robotic hepatectomy and 103 with laparoscopic hepatectomy). The clinical basic information, operation time, intraoperative bleeding, conversion to laparotomy, postoperative hospital stay, anal exhaust time, postoperative eating time, ambulation time, and postoperative hematological indicators were analyzed. Robotic hepatectomy has certain advantages in shortening the length of hospital stay (4.9 ​± ​1.3 VS 6.8 ​± ​2.2 days, P ​

Objective: This study aims to analyze a severe adverse reaction of pulmonary fibrosis induced by dronedarone hydrochloride tablets, and to provide a reference for clinical rational medication through drug precautions. Methods: A case of pulmonary fibrosis induced by dronedarone hydrochloride tablets, along with related literature was retrospectively analyzed. Results: Patients over 65 years old with a history of exposure to amiodarone may increase the incidence of pulmonary toxicity induced by dronedarone, and dronedarone should not be selected as a substitute treatment drug for patients with amiodarone-induced pulmonary toxicity. Conclusions: It is recommended that clinicians monitor the diffusion capacity of carbon monoxide and lung ventilation function of patients before and after using dronedarone for treatment. For patients with a history of amiodarone exposure, intermittent monitoring of chest X-rays and lung function is necessary. If lung function decreases, dronedarone should be immediately discontinued.

Abstract Background Hepatic ischemia-reperfusion injury (IRI) is considered as an effecting factor for hepatocellular carcinoma (HCC) recurrence. Th17/Treg cells are a pair of essential components in adaptive immune response in liver IRI, and forkhead box O1 (FOXO1) has the properties of maintaining the function and phenotype of immune cells. Herein, we illuminated the correlation and function between Th17/Treg cell balance and FOXO1 in IRI-induced HCC recurrence. Methods RNA sequencing was performed on naive CD4+ T cells from normal and IRI model mice to identify relevant transcription factors. Western blotting, qRT-PCR, immunohistochemical staining, and flow cytometry were performed in IRI models to indicate the effect of FOXO1 on the polarization of Th17/Treg cells. Then, transwell assay of HCC cell migration and invasion, clone formation, wound healing assay, and Th17 cells adoptively transfer was utilized to assess the function of Th17 cells in IRI-induced HCC recurrence in vitro and in vivo. Results Owning to the application of RNA sequencing, FOXO1 was screened and assumed to perform a significant function in hepatic IRI. The IRI model demonstrated that up-regulation of FOXO1 alleviated IR stress by attenuating inflammatory stress, maintaining microenvironment homeostasis, and reducing the polarization of Th17 cells. Mechanistically, Th17 cells accelerated IRI-induced HCC recurrence by shaping the hepatic pre-metastasis microenvironment, activating the EMT program, promoting cancer stemness and angiogenesis, while the upregulation of FOXO1 can stabilize the liver microenvironment homeostasis and alleviate the negative effects of Th17 cells. Moreover, the adoptive transfer of Th17 cells in vivo revealed its inducing function in IRI-induced HCC recurrence. Conclusions These results indicated that FOXO1-Th17/Treg axis exerts a crucial role in IRI-mediated immunologic derangement and HCC recurrence, which could be a promising target for reducing the HCC recurrence after hepatectomy. Liver IRI affects the balance of Th17/Treg cells by inhibiting the expression of FOXO1, and the increase of Th17 cells has the ability to induce HCC recurrence through EMT program, cancer stemness pathway, the formation of premetastatic microenvironment and angiogenesis.

Hepatocellular carcinoma (HCC) is a rapidly progressing cancer and the main reason for cancer-related deaths. There are numerous risk factors for HCC, of which hepatitis B virus (HBV) infection is recognized as a high risk. HBV infection is accompanied by gene integration, and the liver has undergone the process of continuous and repeated damage and repair. However, predictive factors of HBV-related HCC are still limited, and the prognostic regulatory genes have not been fully elucidated. This study aims to use bioinformatics analysis to search potential prognostic genes of HBV-related HCC. Based on the full utilization of the GEO database, we screened out prognostic-related genes by performing systematic Kaplan-Meier survival analysis. The differences of the transcriptional information and protein expression were verified in the TCGA and HPA databases respectively, and the clinical characteristics of the screened genes were described by the boxplot. Five prognostic-related genes we screened, including CDK1, MAD2L1, SPP1, TYMS, and CCNA2, are strongly linked with poor prognosis in HBV-related HCC. The five prognostic-related genes have realistic clinical significance and potential as prognostic markers, and may provide new directions for basic research and clinical diagnosis.

In this study examining the effects of temperature and dissolved oxygen (DO) on arsenic (As) release at the sediment–water interface (SWI), it was found that an increase in temperature promoted the formation of an anaerobic environment and the reduction and desorption of As fractions within the sediments. A temperature of 32 °C was the most favorable condition for As release at the SWI, and low DO conditions aggravated this process. Even under high DO conditions, the release of sediment As was significantly accelerated under high-temperature conditions, allowing dissolved As to rapidly migrate to the overlying water. In this process, the release of As from sediments was a consequence of the transformation of As fractions in the sediments.

Mapping brain connections is critical for decoding brain functions. Here, the authors present an integrated resource of awake resting-state fMRI and neuronal tracing data of marmosets to understand structural-functional relationships of brain connections.

Among various sunscreen materials, zinc oxide and titanium dioxide are excellent physical sunscreen components; however, these two materials tend to aggregate and form micrometer-sized particles that may impact their performance. This study utilizes fine grinding techniques to break up the aggregated oxide particles into small nanoparticles with a narrow size distribution averaging around 200 nm in diameter. The ground zinc oxide and titanium dioxide particles exhibit enhanced ability in absorbing and scattering ultraviolet radiation compared to their original state before grinding. Consequently, this research offers innovative concepts as well as approaches towards developing highly efficient yet low-toxic physical sunscreen components while significantly contributing to the preparation process optimization for mixed material performance.

Abstract Non‐alcoholic fatty liver disease (NAFLD) is a typical chronic liver disease connected to a high risk of developing hepatocellular carcinoma (HCC). The development of NAFLD and HCC has been associated with changes in epigenetics, such as histone modifications and micro RNA (miRNA)‐mediated processes. Recently, in the realm of epitranscriptomics, RNA alterations have become important regulators. N6‐methyladenosine (m6A) is the most common and crucial alteration for controlling mRNA stability, splicing, and translation. It is particularly important for controlling liver disease progression and hepatic function. This review aims to conclude recent research on the functions of m6A epitranscriptome in the molecular mechanisms behind NAFLD and HCC development, with special attention to the effects of m6A alteration on how HCC develops and its possible roles in the progression of NAFLD to HCC. Additionally, the review discusses the possible effects of m6A alteration on the treatment and diagnostic of NAFLD and HCC. It is crucial to remember that m6A modification is a reversible action controlled via the coordinated functions of the proteins that write and delete, enabling quick adaptability to environmental changes. The review also discusses m6A‐binding proteins' function in mRNA alternative splicing, translation, and degradation and their ability to modulate mRNA stability and processing. Understanding RNA modification regulation and its part in the emergence of HCC and NAFLD may provide new avenues for diagnosing and treating these diseases.

Abstract Background The effectiveness of laparoscopic total gastrectomy with D2 lymphadenectomy (LTGD2) remains controversial. This meta-analysis compares surgical and survival outcomes of LTGD2 and open total gastrectomy with D2 lymphadenectomy (OTGD2) for gastric cancer. Methods Controlled studies comparing LTGD2 and OTGD2 published before November 2021 were retrieved via database searches. We compared intraoperative outcomes, pathological data, postoperative outcomes, 5-year disease-free survival (DFS), and overall survival (OS). Results 17 studies were included, containing 4742 patients. Compared with OTGD2, the LTGD2 group had less blood loss (mean difference [MD] = − 122.48; 95% CI: − 187.60, − 57.37; P = 0.0002), fewer analgesic medication (MD = -2.48; 95% CI: − 2.69, − 2.27; P

Terrace detection and ridge extraction from high-resolution remote sensing imagery are crucial for soil conservation and grain production on sloping land. Traditional methods use low-to-medium resolution images, missing detailed features and lacking automation. Terrace detection and ridge extraction are closely linked, with each influencing the other’s outcomes. However, most studies address these tasks separately, overlooking their interdependence. This research introduces a cutting-edge, multi-scale, and multi-task deep learning framework, termed DTRE-Net, designed for comprehensive terrace information extraction. This framework bridges the gap between terrace detection and ridge extraction, executing them concurrently. The network incorporates residual networks, multi-scale fusion modules, and multi-scale residual correction modules to enhance the model’s robustness in feature extraction. Comprehensive evaluations against other deep learning-based semantic segmentation methods using GF-2 terraced imagery from two distinct areas were undertaken. The results revealed intersection over union (IoU) values of 85.18% and 86.09% for different terrace morphologies and 59.79% and 73.65% for ridges. Simultaneously, we have confirmed that the connectivity of results is improved when employing multi-task learning for ridge extraction compared to directly extracting ridges. These outcomes underscore DTRE-Net’s superior capability in the automation of terrace and ridge extraction relative to alternative techniques.

With the growing interest in radiant heating and cooling systems, driven by their improved efficiency and enhanced thermal comfort compared to air systems, there is an increasing need to develop a more accessible method for designers to understand the implications of radiation heat exchange between the human body and radiant panels. To address this, a novel angle factor calculation model, named the HNU Angle Factor Model, was developed, taking into account the spatial arrangement and geometric relationship between the human body and radiant panels. The angle factors obtained using the HNU Angle Factor Model exhibited good agreement with the results obtained with Fanger diagrams and the contour integral method, with average relative differences of 8.1% and 10.0% for 140 cases, respectively. Furthermore, placing a radiant panel on the floor while maintaining its fixed size can contribute to the creation of an even and efficient thermal environment for individuals in both seated and standing positions. By implementing the HNU Angle Factor Model in practical engineering applications, more effective utilization of radiant systems can be achieved, as it provides an evaluation of the heat transfer between the human body and radiant panels.

Abstract Characterized by hepatic lipid accumulation, non‐alcoholic fatty liver disease (NAFLD) is a multifactorial metabolic disorder that could promote the progression of non‐alcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). Benefiting from recent advances in omics technologies, such as high‐throughput sequencing, voluminous profiling data in HCC‐integrated molecular science into clinical medicine helped clinicians with rational guidance for treatments. In this review, we conclude the majority of publicly available omics data on the NAFLD‐related disease spectrum and bring up new insights to inspire next‐generation therapeutics against this increasingly prevalent disease spectrum in the post‐genomic era.

Introduction Identifying an underlying germline cancer predisposition (CP) in a child with cancer has potentially significant implications for both the child and biological relatives. Cohort studies indicate that 10%–15% of paediatric cancer patients carry germline pathogenic or likely pathogenic variants in cancer predisposition genes, but many of these patients do not meet current clinical criteria for genetic testing. This suggests broad tumour agnostic germline testing may benefit paediatric cancer patients. However, the utility and psychosocial impact of this approach remain unknown. We hypothesise that an approach involving trio whole-genome germline sequencing (trio WGS) will identify children and families with an underlying CP in a timely fashion, that the trio design will streamline cancer risk counselling to at-risk relatives if CP was inherited, and that trio testing will not have a negative psychosocial impact on families.Method and analysis To test this, we present the Cancer PREDisposition In Childhood by Trio sequencing study (PREDICT). This study will assess the clinical utility of trio WGS to identify CP in unselected patients with cancer 21 years or younger in New South Wales, Australia. PREDICT will perform analysis of biological parents to determine heritability and will examine the psychosocial impact of this trio sequencing approach. PREDICT also includes a broad genomics research programme to identify new candidate genes associated with childhood cancer risk.Ethics and dissemination By evaluating the feasibility, utility and psychosocial impact of trio WGS to identify CP in paediatric cancer, PREDICT will inform how such comprehensive testing can be incorporated into a standard of care at diagnosis for all childhood cancer patients.Trial registration number NCT04903782.

Reference evapotranspiration (ET0) is one of the significant parameters in agricultural irrigation, especially in Heilongjiang, a big agricultural province in China. In this research, the spatiotemporal variation characteristics of evapotranspiration (ET), relative moisture index (MI) and influencing factors of ET0 in Heilongjiang, which was divided into six ecology districts according to landforms, were analyzed with meteorological data observed over 40 years from 1980 and MOD16 products from 2000 to 2017 using Morlet wavelet analysis and partial correlation analysis. The results indicated that (1) the spatial distribution of ET and PET in Heilongjiang in humid, normal and arid years showed a distribution of being higher in the southwest and lower in the northwest, and higher in the south and lower in the north. The PET was higher than ET from 2002 to 2017, and the difference was small, indicating that the overall moisture in Heilongjiang was sufficient in these years. (2) In the last 40 years, the ET0 increased while the annual MI decreased. The annual minimum of MI in the six regions of Heilongjiang was −0.25, showing that all six regions were drought free. (3) The importance of the meteorological factors affecting ET0 was ranked as average relative humidity > average wind speed > sunshine duration. This research provides scientific guidance for the study of using remote sensing to reverse ET.

Our brains constantly generate predictions of sensory input that are compared with actual inputs, propagate the prediction-errors through a hierarchy of brain regions, and subsequently update the internal predictions of the world. However, the essential feature of predictive coding, the notion of hierarchical depth and its neural mechanisms, remains largely unexplored. Here, we investigated the hierarchical depth of predictive auditory processing by combining functional magnetic resonance imaging (fMRI) and high-density whole-brain electrocorticography (ECoG) in marmoset monkeys during an auditory local-global paradigm in which the temporal regularities of the stimuli were designed at two hierarchical levels. The prediction-errors and prediction updates were examined as neural responses to auditory mismatches and omissions. Using fMRI, we identified a hierarchical gradient along the auditory pathway: midbrain and sensory regions represented local, shorter-time-scale predictive processing followed by associative auditory regions, whereas anterior temporal and prefrontal areas represented global, longer-time-scale sequence processing. The complementary ECoG recordings confirmed the activations at cortical surface areas and further differentiated the signals of prediction-error and update, which were transmitted via putative bottom-up γ and top-down β oscillations, respectively. Furthermore, omission responses caused by absence of input, reflecting solely the two levels of prediction signals that are unique to the hierarchical predictive coding framework, demonstrated the hierarchical top-down process of predictions in the auditory, temporal, and prefrontal areas. Thus, our findings support the hierarchical predictive coding framework, and outline how neural networks and spatiotemporal dynamics are used to represent and arrange a hierarchical structure of auditory sequences in the marmoset brain.

During the past four decades, China has gone through rapid urbanization and modernization. As people adapt to dramatic sociodemographic shifts from rural communities to urban centers and as economic level rises, individualistic cultural values in China have increased. Meanwhile, parent and child behavior in early childhood has also evolved accordingly to match a more individualistic society. This mixed-method study investigated how social change in China may have impacted parenting goals and child development in middle childhood, as seen through the eyes of the current generation of mothers. Thirty mothers of fifth-grade elementary school students from Shenzhen, China were recruited and took part in semi-structured interviews. Participants answered questions and provided examples about their children’s life, their own childhood, and the perceived differences between the two generations. Participating mothers were also asked to rate which generation, themselves or their parents, cared more about the childrearing goals of academic competitiveness and socioemotional well-being. Using both qualitative and quantitative analysis, we expected and found an intergenerational increase in the perceived value mothers placed on individualistic traits: current mothers care more about their children’s academic competitiveness, personal happiness, and social adjustment, compared to their experience of their own mothers’ attitudes during their childhood a generation earlier. They also experience conflict between their children’s academic competitiveness and socioemotional well-being. As a function of both urbanization and increased economic means, children’s collectivistic family responsibilities for essential household chores have declined as the importance of schoolwork has increased.

With the increase of the renewable energy penetration (REP) level in the interconnected power grid, the proportion of the grid-connected conventional synchronous generators reduces continuously, resulting in the decrease of the system inertia. The insufficient system inertia brings challenges to the system frequency stability. Battery energy storage systems (BESSs), regarded as the high-quality frequency regulation resource, play an important role in maintaining the frequency stability of the system with the high REP level. To configure the proper power of BESSs in system frequency regulation, a BESS power configuration scheme (PCS) considering the REP constraint is proposed in this paper. In particular, the process to obtain the REP boundary of the interconnected grid on the premise of system frequency stability is included in the PCS, and the optimal power configuration of the BESS is further determined on the analysis of the BESS impact on the REP boundary. Furthermore, a simulation model of the Australian five-area interconnected power grid is built in MATLAB/Simulink, and the proposed REP-constrained PCS is verified and analyzed. At last, the promising results show that the PCS can take full advantages of the BESS in frequency regulation and meet the system requirement of the frequency stability at a particular REP level.

Objectives: Preoperative prediction of post-hepatectomy liver failure (PHLF) in patients with hepatocellular carcinoma (HCC) is significant for developing appropriate treatment strategies. We aimed to establish a radiomics-based clinical model for preoperative prediction of PHLF in HCC patients using gadolinium-ethoxybenzyl-diethylenetriamine (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI).Methods: A total of 144 HCC patients from two medical centers were included, with 111 patients as the training cohort and 33 patients as the test cohort, respectively. Radiomics features and clinical variables were selected to construct a radiomics model and a clinical model, respectively. A combined logistic regression model, the liver failure (LF) model that incorporated the developed radiomics signature and clinical risk factors was then constructed. The performance of these models was evaluated and compared by plotting the receiver operating characteristic (ROC) curve and calculating the area under the curve (AUC) with 95% confidence interval (CI).Results: The radiomics model showed a higher AUC than the clinical model in the training cohort and the test cohort for predicting PHLF in HCC patients. Moreover, the LF model had the highest AUCs in both cohorts [0.956 (95% CI: 0.955–0.962) and 0.844 (95% CI: 0.833–0.886), respectively], compared with the radiomics model and the clinical model.Conclusions: We evaluated quantitative radiomics features from MRI images and presented an externally validated radiomics-based clinical model, the LF model for the prediction of PHLF in HCC patients, which could assist clinicians in making treatment strategies before surgery.

Nifedipine is completely absorbed by the gastrointestinal tract and its pharmacokinetics and metabolism may be influenced by microorganisms. If gut microbes are involved in the metabolism of nifedipine, plateau hypoxia may regulate the bioavailability and the therapeutic effect of nifedipine by altering the metabolic activity of the gut microbiota. We herein demonstrated for the first time that gut flora is involved in the metabolism of nifedipine by in vitro experiments. In addition, based on the results of 16S rRNA analysis of feces in rats after acute plateau, we first confirmed that the plateau environment could cause changes in the number and composition of intestinal microbes. More importantly, these changes in flora could lead to a slower metabolic activity of nifedipine in the body after an acute plateau, resulting in increased bioavailability and therapeutic efficacy of nifedipine. Our research will provide basis and new ideas for changes in the fecal flora of human acutely entering the plateau, and contribute to rational drug use of nifedipine.