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150 results on '"Zhang, L.P."'

24. Characterization of metallothioneins (MT-I and MT-II) in the yak

Author

Wu, J.P., Ma, B.Y., Ren, H.W., Zhang, L.P., Xiang, Y., and Brown, M.A.

Subjects
Yaks -- Genetic aspects, Yaks -- Research, Metallothionein -- Research, Nucleotides -- Research, Zoology and wildlife conservation
Abstract

The cDNA-encoding sequences for yak metallothionein isoforms I (MT-I) and II (MT-II) were amplified and cloned by reverse-transcription PCR to characterize the nucleotide sequence and protein structure of metallothionein in the yak. The cDNA sequences of MT-I and MT-II were subjected to BLAST searching at the National Center for Biotechnology Information, and the results indicated that the nucleotide sequences of yak MT-I and MT-II, when compared among different species of mammals, are highly conserved. The yak open reading frames have a length of 183 nucleotides, which encode for yak MT-I and MT-II proteins of 61 AA, respectively. Analysis of hydrophobicity, transmembrane region, and signal peptides suggested that metallothioneins of the yak are nonsecretory proteins. There were several conserved tripeptide sequences, such as C-X-C, C-C-X-C-C, and C-X-X-C (X designates AA excluding cysteine in MT-I and MT-II), and they are highly conserved in their evolution. By homologous comparative modeling, we predicted the molecular spatial structures of yak MT-I and MT-II, which are composed of [alpha]- and [beta]-domains that are linked by the conserved tripeptide [Lys.sup.30]-[Lys.sup.30] -[Ser.sup.32] (KKS). Key words: cDNA, characterization, metallothioneins, structure, yak

Published
2007

28. Benzene induces gene-duplicating but not gene-activating mutations at the glycophorin A locus in exposed humans

Author

Rothman, N., Haas, R., Hayes, R.B., G.-L. Li, Wiemels, J., Campleman, S., Quintana, P.J.E., Xi, L.-J., Dosemeci, M., Titenko-Holland, N., Meyer, K.B., Lu, W., Zhang, L.P., Bechtold, W., Wang, Y.-Z., Kolachana, P., Yin, S.-N., Blot, W., and Smith, M.T.

Subjects
Leukemia research -- Reports, Mutation (Biology) -- Analysis, Bone marrow examination -- Reports, Science and technology
Abstract

In human bone marrow cells, benzene develops gene-duplicating mutations at the glycophorin A (GPA) locus. Enhanced exposure to benzene increases the cell frequency of the surface protein with double N expression (NN), but not of the surface protein with single N. Duplication of the N allele and loss of the GPA M allele produces the NN variant. The surface protein with single N allele results from gene inactivation.

Published
1995

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