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Tuberculosis (TB) is a serious and fatal disease caused by Mycobacterium tuberculosis (Mtb). The World Health Organization reported an estimated 1.30 million TB-related deaths in 2022. The escalating prevalence of Mtb strains classified as being multi-, extensively, extremely, or totally drug resistant, coupled with the decreasing efficacies of conventional therapies, necessitates the development of novel treatments. As viruses that infect Mycobacterium spp. mycobacteriophages may represent a strategy to combat and eradicate drug-resistant TB. More exploration is needed to provide a comprehensive understanding of mycobacteriophages and their genome structure, which could pave the way toward a definitive treatment for TB. This review focuses on the properties of mycobacteriophages, their potential in diagnosing and treating TB, the benefits and drawbacks of their application, and their use in human health. Specifically, we summarize recent research on mycobacteriophages targeted against Mtb infection and newly developed mycobacteriophage-based tools to diagnose and treat diseases caused by Mycobacterium spp. We underscore the urgent need for innovative approaches and highlight the potential of mycobacteriophages as a promising avenue for developing effective diagnosis and treatment to combat drug-resistant Mycobacterium strains. [ABSTRACT FROM AUTHOR]

Memorial facilities are one of the crucial places where citizens conduct activities facilitating disaster risk reduction (DRR). However, previous studies have primarily focused on the post-construction phase of official memorial facilities, neglecting the citizen activities collaborating with the official memorial construction process before and during the construction process. This research gap is important considering the urgency of disaster-affected regions to recover from spatial, social, and psychological voids while working towards the goal of DRR, including the efforts of citizens in the official efforts. This study addresses this gap by examining the case of the official memorial park in Ishinomaki, Tohoku region, following the Great East Japan Earthquake. Here, local citizens actively participated in activities before, during, and after park construction, engaging with official efforts. Data were gathered from various online sources to capture activity, space, and management information. Employing a mixed methods research approach, we conducted both quantitative analysis, counting labels of structural coding, and qualitative description of original texts. Our findings reveal that fostering mutual respect built on communication and collaborative tree-planting activities were crucial for maintaining the pre-existing citizen activities and collaborative construction during the official construction period. Additionally, the implementation of a collaborative regulation system was vital for integrating and managing spontaneous citizen activities to achieve the park's intended objectives post-opening. In conclusion, we highlighted a framework elucidating the mechanisms through which these processes contribute to DRR across key phases of disaster risk management: preparedness, prevention, response, and recovery (PPRR). These insights are important in guiding efforts to engage citizens in DRR initiatives through recovery and reconstruction facilitated by memorial facilities. [ABSTRACT FROM AUTHOR]

Simultaneously quantifying mitochondrial Cu+ and Cu2+ levels is crucial for evaluating the molecular mechanisms of copper accumulation-involved pathological processes. Here, a series of molecules containing various diacetylene derivatives as Raman reporters are designed and synthesized, and the alkyne-tagged SERS probe is created for determination Cu+ and Cu2+ with high selectivity and sensitivity. The developed SERS probe generates well-separated distinguishable Raman fingerprint peaks with built-in corrections in the cellular silent region, resulting in accurate quantification of Cu+ and Cu2+. The present probe demonstrates high tempo-spatial resolution for real-time imaging and simultaneously quantifying mitochondrial Cu+ and Cu2+ with long-term stability benefiting from the probe assembly with designed Au-C≡C groups. Using this powerful tool, it is found that mitochondrial Cu+ and Cu2+ increase during ischemia are associated with breakdown of proteins containing copper as well as conversion of Cu+ and Cu2+. Meanwhile, we observe that parts of Cu+ and Cu2+ are transported out of neurons by ATPase. More importantly, cuproptosis in neurons is found including the oxidative stress process caused by the conversion of Cu+ to Cu2+, which dominates at the early stage (<9 h), and subsequent proteotoxic stress. Both oxidative and proteotoxic stresses contribute to neuronal death. Simultaneously quantifying mitochondrial Cu+ and Cu2+ levels is vital for understanding the molecular mechanism of mitochondria-related biological events. Here the authors report an alkynyl-labeled SERS probe to simultaneously monitor free Cu+ and Cu2+ in mitochondria, and unveil their roles during ischemia and cuproptosis processes. [ABSTRACT FROM AUTHOR]

Diabetes mellitus (DM) has been proposed to be positively associated with breast cancer (BCa) risk due to shared risk factors, metabolic dysfunction, and the use of antidiabetic medications. We conducted a systematic review and meta‐analysis to evaluate the association between DM and BCa risk. We searched PubMed, Embase, and Web of Science for cohort and case‐control studies assessing the association between DM and BCa published before 10 December 2021. Two reviewers independently screened the studies for inclusion, abstracted article data, and rated study quality. Random effects models were used to estimate summary risk ratios (RRs) and 95% confidence intervals (CIs). From 8396 articles identified in the initial search, 70 independent studies were included in the meta‐analysis. DM was associated with an overall increased risk of BCa (RR = 1.20, 95% CI: 1.11–1.29). The 24 case‐control studies demonstrated a stronger association (RR = 1.26, 95% CI: 1.13–1.40) than the 46 cohort studies (RR = 1.15, 95% CI: 1.05–1.27). Studies reporting risk by menopausal status found that postmenopausal women had an elevated risk of developing BCa (RR = 1.12, 95% CI: 1.07–1.17). No association between DM and BCa risk was observed among premenopausal women (RR = 0.95, 95% CI: 0.85–1.05). In addition, DM was associated with significantly increased risks of oestrogen receptor (ER)+ (RR = 1.09, 95% CI: 1.00–1.20), ER‐ (RR = 1.16, 95% CI: 1.04–1.30), and triple negative BCa (RR = 1.41, 95% CI: 1.01–1.96). The association estimate for human epidermal growth factor 2‐positive BCa was also positive (RR = 1.21, 95% CI: 0.52–2.82), but the CI was wide and crossed the null. Our meta‐analysis confirms a modest positive association between DM and BCa risk. In addition, our results suggest that the association between DM and BCa may be modified by menopausal status, and that DM may be differentially associated with BCa subtypes defined by receptor status. Additional studies are warranted to investigate the mechanisms underlying these associations and any influence of DM on BCa receptor expression. [ABSTRACT FROM AUTHOR]