22 results on '"Zhengchun Wu"'
Search Results
2. Understanding Water Utilization Mechanisms in Degrading Bamboo Shoots: A Cytological and Physiological Study
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Tianyi Hu, Zhengchun Wu, Meng Deng, Haiwen Liu, Jiao Xiao, Qiang Wei, and Fen Yu
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Phyllostachys edulis ‘Pachyloen’ ,degrading shoots ,parent bamboo ,water pressure ,water potential ,programmed cell death ,Botany ,QK1-989 - Abstract
Degradation of shoots, characterized by stunted growth and signs of water deficit, is common in bamboo stands. However, the specific mechanisms underlying water utilization in degrading shoots remain unclear. This study sought to address this gap by harvesting bamboo shoots and culms of Phyllostachys edulis ‘Pachyloen’, employing cytological and physiological techniques to compare water utilization mechanisms between healthy and degrading shoots, and investigating the water supply to bamboo shoots by the parent bamboo. The water pressure in the degrading shoots was markedly lower compared to that of the healthy shoots, and it declined as the degradation progressed, resulting in reduced water content and the cessation of guttation in the degrading shoots. In conditions of water deficit, the percentage of free water in bamboo shoots decreased while the percentages of bound and semi-bound water increased, with the proportion of semi-bound water reaching as high as 88.13% in the late stages of degradation. The water potential of parent bamboo culms of different ages varied at different times of the day and during different growth stages of bamboo shoots, showing a strong association with the development of bamboo shoots. Conversely, the correlation between changes in the water potential of bamboo shoots and their degradation patterns was found to be comparatively minimal. The weakening of the connection between the bamboo shoots and the parent bamboo culms may play a significant role in the degradation of the bamboo shoots. This is evidenced by a decrease in the fluorescence intensity of the nucleus in bamboo shoots and the degradation of genetic material. This study lays the foundation for future research into the mechanisms of bamboo shoot degradation.
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- 2024
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3. Supplementing a specific synbiotic suppressed the incidence of AOM/DSS-induced colorectal cancer in mice
- Author
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Huixia Wu, Zhengchun Wu, Yilan Qiu, Fangjian Zhao, Minjing Liao, Zhihong Zhong, Jian Chen, Yiliang Zeng, and Rushi Liu
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Cancer ,Cancer systems biology ,Molecular biology ,Molecular medicine ,Science - Abstract
Summary: In this study, we evaluated the effect of a specific synbiotic on CAC (AOM/DSS-induced colitis-associated cancer). We confirmed that the synbiotic intervention was able to protect the intestinal barrier and inhibit CAC occurrence via upregulating tight junction proteins and anti-inflammatory cytokines, and downregulating pro-inflammatory cytokines. Moreover, the synbiotic significantly improved the disorder of the colonic microbiota of CAC mice, promoted the formation of SCFAs and the production of secondary bile acids, and alleviated the accumulation of primary bile acids in the CAC mice. Meanwhile, the synbiotic could significantly inhibit the abnormal activation of the intestinal Wnt/β-catenin signaling pathway significantly related to IL-23. In a word, the synbiotic can inhibit the occurrence and development of colorectal tumors and it may be a functional food to prevent inflammation-related colon tumors, and the research also provided a theoretical basis for improving the intestinal microecological environment through diet therapy.
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- 2023
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4. Fructooligosaccharides supplementation mitigated chronic stress-induced intestinal barrier impairment and neuroinflammation in mice
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Yuanfang Zhang, Ping Wang, Chuan Xia, Zhengchun Wu, Zhihong Zhong, Ye Xu, Yiliang Zeng, Hongrong Liu, Rushi Liu, and Minjing Liao
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Chronic stress ,Depression ,Gut microbiota ,Prebiotic ,Fructooligosaccharides ,Neuroinflammation ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Recently, accumulating evidence has suggested that gut microbiota is involved in modulating brain function and behavior through the gut-brain axis. Manipulation of gut microbiota by functional foods, such as prebiotics and probiotics, might improve mental health in stress-related psychiatric disorders. In present study, we found that supplementation of fructooligosaccharides could improve the depression-like behaviors and had a significant effect on the composition of gut flora in chronic stress mice. FOS treatment exhibited no effects on the elevated level of serum corticosterone in chronic stress mice, but significantly reversed the alterations of colonic tight junction proteins (Claudin1, Occludin, ZO-1) and reduced the pro-inflammatory cytokines (TNF-α, IL-6) in colon and brain. Thus, FOS might exert an anti-depressant effect via the prevention of intestinal barrier impairment and neuroinflammation induced by chronic stress.
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- 2020
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- View/download PDF
5. Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer
- Author
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Rushi Liu, Zhengchun Wu, Yuanfang Zhang, Xiongying Miao, Qiong Zou, Yuan Yuan, Daiqiang Li, and Zhulin Yang
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gallbladder cancer ,X-box-binding protein 1 ,N-acetyltransferase 1 ,prognosis ,clinicopathological significance ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: X-box-binding protein 1 (XBP1) and N-acetyltransferase 1 (NAT1) are involved in oncogenesis and progression of many human cancer types. However, the roles of XBP1 and NAT1 in gallbladder cancer (GBC) are never reported.Methods: We examined XBP1 and NAT1 expression in GBC and matched adjacent non-tumor tissues via Western blotting. Then, we assayed XBP1 and NAT1 expression in 215 GBCs, including 69 squamous cell/adenosquamous carcinomas (SC/ASCs) and 146 adenocarcinomas (ACs) with immunohistochemistry. Their prognostic and clinicopathological significance was further evaluated using the χ2 test or Fisher's exact test, Kaplan–Meier univariate survival analysis, and log-rank tests.Results: XBP1 expression was upregulated, and NAT1 expression was downregulated in GBC. Immunohistochemical results showed that XBP1 expression was negatively associated with NAT1 expression in GBC, including SC/ASC and AC. The rate of patients with an age of more than 45 years, positivity of lymph node metastasis, and invasion were significantly higher in SC/ASC than those in AC (all P < 0.05). The percentage of XBP1-positive and NAT1-negative expression was significantly higher in the cases with poor differentiation, advanced tumor, nodes, and metastases (TNM) stage, lymph node metastasis, invasion, and only receiving biopsy in GBC, SC/ASC, and AC (all P < 0.05). XBP1-positive and NAT1-negative expression was positively related to larger tumor size (>3 cm) in GBC and AC. There was a negative association between XBP1 and NAT1 expression in GBC, SC/ASC, and AC (all P < 0.05). Positive XBP1 and negative NAT1 expression was closely associated with decreased overall survival in GBC, SC/ASC, and AC patients (all P < 0.05). The multivariate Cox regression analysis showed that positive XBP1 or negative NAT1 expression was an independent factor for poor prognosis in gallbladder SC/ASC and AC patients.Conclusions: This study indicates that positive XBP1 and negative NAT1 expression are closely associated with the clinicopathological and biological behaviors and poor prognosis in GBC.
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- 2020
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6. XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer
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Zhengchun Wu, Xiongying Miao, Yuanfang Zhang, Daiqiang Li, Qiong Zou, Yuan Yuan, Rushi Liu, and Zhulin Yang
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XRCC1 ,gallbladder cancer ,prognosis ,clinicopathological significance ,chemo-resistance ,Biology (General) ,QH301-705.5 - Abstract
Gallbladder cancer is a relatively uncommon human malignant tumor with an extremely poor prognosis. Currently, no biomarkers can accurately diagnose gallbladder cancer and predict patients’ prognosis. XRCC1 is involved in tumorigenesis, progression, and chemo-resistance of several human cancers, but the role of XRCC1 in gallbladder cancer is never reported. In this study, we investigated the expression of XRCC1 and its clinicopathological and prognostic significance in gallbladder cancer, and explored the biological role of XRCC1 in gallbladder cancer cells. We found that XRCC1 was significantly up-regulated in gallbladder cancer in protein and mRNA levels. Positive XRCC1 expression was correlated with aggressive clinicopathological features and was an independent poor prognostic factor in gallbladder cancer. The ROC curves suggested that XRCC1 expression had potential clinicopathological diagnostic value in gallbladder cancer. In vitro, XRCC1 was overexpression in CD133+GBC-SD cells compared to GBC-SD cells. In functional experiment, XRCC1 knockdown had a non-significant impact on proliferation, migration, invasion, and apoptosis of CD133+GBC-SD cells. But, XRCC1 knockdown could significantly improve the sensitivity of CD133+GBC-SD cells to 5-Fluorouracil via promoting cell necrosis and apoptosis. Thus, this study indicates that XRCC1 may be a promising predictive biomarker of gallbladder cancer and a potential therapeutic target for gallbladder cancer.
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- 2020
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7. Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma
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Lile He, Xiangyu Shi, Ruiqi Chen, Zhengchun Wu, Zhulin Yang, and Zhihong Li
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DAXX ,DRD3 ,DISC1 ,immunohistochemistry ,chondrosarcoma ,osteochondroma ,Biology (General) ,QH301-705.5 - Abstract
Chondrosarcoma is the second most common malignant bone tumor. Current therapies remain ineffective, resulting in poor prognoses. Biomarkers for chondrosarcoma and predictors of its prognosis have not been established. Mental health–related proteins have been associated with the pathogenesis, progression, and prognosis of many cancers, but their association with chondrosarcoma has not been reported. In this study, the expression and clinicopathological significance of the mental health–related proteins DAXX, DRD3, and DISC1 in chondrosarcoma tissue samples were examined, over an 84-months follow-up period. In immunohistochemical analysis, the rates of positive DAXX, DRD3, and DISC1 expression were significantly higher in chondrosarcoma than in osteochondroma tissue (P < 0.01). The percentages of positive DAXX, DRD3, and DISC1 expression were significantly lower in tissues with good differentiation (P < 0.01), AJCC stage I/ II (P < 0.01), Enneking stage I (P < 0.01), and non-metastasis (P < 0.05), respectively. In Kaplan–Meier survival analysis, significantly shorter mean survival times were associated with moderate and poor differentiation (P = 0.000), AJCC stage III/IV (P = 0.000), Enneking stage II/III (P = 0.000), metastasis (P = 0.019), invasion (P = 0.013), and positive DAXX (P = 0.012), and/or DRD3 (P = 0.018) expression. In Cox regression analysis, moderate and poor differentiation (P = 0.006), AJCC stage III/IV (P = 0.013), Enneking stage II/III (P = 0.016), metastasis (P = 0.033), invasion (P = 0.011), and positive DAXX (P = 0.033), and/or DRD3 (P = 0.025) staining correlated negatively with the postoperative survival rate and positively with mortality. In competing-risks regression analysis, differentiation (P = 0.005), metastasis (P = 0.014), invasion (P = 0.028), AJCC stage (P = 0.003), Enneking stage (P = 0.036), and DAXX (P = 0.039), and DRD3(P = 0.019) expression were independent predictors of death from chondrosarcoma. The areas under receiver operating characteristic curves for DAXX, DRD3, and DISC1 expression were 0.673 (95% CI, 0.557–0.788; P = 0.010), 0.670 (95% CI, 0.556–0.784; P = 0.011), and 0.688 (95% CI, 0.573–0.802; P = 0.005), respectively. These results suggest that DAXX, DRD3, and DISC1 could serve as biomarkers of chondrosarcoma progression and predictors of its prognosis.
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- 2019
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8. Expression of UGP2 and CFL1 expression levels in benign and malignant pancreatic lesions and their clinicopathological significance
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Lingxiang Wang, Li Xiong, Zhengchun Wu, Xiongying Miao, Ziru Liu, Daiqiang Li, Qiong Zou, and Zhulin Yang
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Pancreatic ductal adenocarcinoma ,Chronic pancreatitis ,Pancreatic intraepithelial neoplasia ,UGP2 ,CFL1 ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background This study investigated UGP2 (uridine diphosphate-glucose pyrophosphorylase-2) and CFL1 (cofilin-1) expression in pancreatic ductal carcinoma (PDC), paracancerous tissue (PT), benign lesions (BL), and normal tissue (NT) and their clinicopathological significance. Methods Surgical specimens, which were collected from 106 cases of pancreatic ductal carcinoma, 35 cases of paracancerous tissues, 55 cases of benign lesions and 13 cases of normal pancreatic tissues, were fixed with 4% formaldehyde to prepare conventional paraffin-embedded sections. EnVision immunohistochemical was used to stain for UGP2 and CFL1. Kaplan-Meier survival analysis was performed to assess the correlation of expression pattern with survival. Results We found that positive UGP2 and CFL1 expression in PDC were significantly higher than those in PT, BL, and NT. In PT and BL with positive UGP2 and CFL1 expression, mild to severe atypical hyperplasia or intraepithelial neoplasia of grades II–III was observed in ductal epithelium. Positive UGP2 and CFL1 expression in cases with high differentiation, no lymph node metastasis, no surrounding invasion, and TNM (tumor-node-metastasis) staging I or/and II were significantly lower than those in cases with poor differentiation, lymph node metastasis, surrounding invasion, and TNM stage III and/or IV. Positive UGP2 expression in male patients was significantly lower than that in female patients. UGP2 and CFL1 expression in PDC were positively correlated. Kaplan-Meier survival analysis showed the degree of differentiation, tumor maximal diameter, TNM stage, lymph node metastasis, and surrounding invasion, and UGP2 and CFL1 expression were closely related to the average survival time of patients with PDC. The survival time of patients with positive UGP2 and CFL1 expression was significantly shorter than that of patients with negative expression. Cox multivariate analysis showed that poor differentiation, tumor maximal diameter ≥ 3 cm, TNM stage III or IV, lymph node metastasis, surrounding invasion, and positive UGP2 and CFL1 expression was negatively correlated with the postoperative survival rate and positively correlated with the mortality of patients with PDC. Conclusion Positive expression of UGP2 and CFL1 can serve a valuable prognostic factor in pancreatic cancer.
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- 2018
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9. APEX1 predicts poor prognosis of gallbladder cancer and affects biological properties of CD133+ GBC-SD cells via upregulating Jagged1
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Zhengchun Wu, Ziru Liu, Yi Sun, Yuan Yuan, Qiong Zou, Yun Wen, Jia Luo, and Rushi Liu
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Oncology - Published
- 2023
10. Bufotalin-loaded biomimetic prussian blue nanoparticles for colorectal cancer chemo-photothermal therapy via induce ferroptosis
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Yi Wu, Jiahui Tian, Mengjie Luo, Zhengchun Wu, Ruiyun Cao, Chunyan Fu, Jialu Yang, Qian Peng, Rushi Liu, and Bin Liu
- Abstract
Background: As a ferroptosis-inducing platform, based on its release of iron and ferrous ions to induce production of reactive oxygen species (ROS), it has attracted extensive attention in cancer treatment. Since iron-based nanomaterials such as prussian blue (PB), can only produce limited ROS. Bufotalin (CS-5), a promising ferroptosis inducer, was introduced to improve the efficiency of ferroptosis. However, the clinical application of CS-5 was limited by cardiotoxicity and high renal clearance rate. Therefore, we constructed a biomimetic nanosystem (PB@CS-5@M) with satisfactory tumor enrichment ability and long blood circulation time for the treatment of colorectal cancer induced by ferroptosis. Results: In this work, the PB@CS-5@M has efficient loading of CS-5, which significantly reduces the cardiotoxicity of CS-5. After photothermal therapy, the high temperature caused by laser stimulation triggers the release of CS-5, iron and ferrous ions to kill colorectal cancer cells. Furthermore, the ability of CS-5 to significantly down-regulate the expression of GPX4 and HIF-1a, induce ROS production and damage mitochondria to induce ferroptosis in colorectal cancer was amplified. In addition, the down-regulation of HSP70 expression under laser stimulation effectively improved the chemosensitivity of HCT116 cells to CS-5. Conclusions: The chemotherapeutic effect of CS-5 combined with the photothermal effect of PB can effectively treat colon cancer by inducing ferroptosis.
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- 2023
11. Fructooligosaccharide supplementation alleviated the pathological immune response and prevented the impairment of intestinal barrier in DSS-induced acute colitis mice
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Liao Minjing, Zhengchun Wu, Li Xiong, Xiaoqi Zeng, Zhihong Zhong, Yiliang Zeng, Yuanfang Zhang, Rushi Liu, Yu Wen, and Yilan Qiu
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Male ,medicine.medical_specialty ,Colon ,Oligosaccharides ,Synbiotics ,Inflammation ,Gut flora ,digestive system ,Inflammatory bowel disease ,Mice ,Immune system ,Internal medicine ,Animals ,Medicine ,Intestinal Mucosa ,Acute colitis ,Tight Junction Proteins ,biology ,business.industry ,Probiotics ,Fructooligosaccharide ,Dextran Sulfate ,Immunity ,General Medicine ,Colitis ,Inflammatory Bowel Diseases ,biology.organism_classification ,medicine.disease ,Mucus ,digestive system diseases ,Gastrointestinal Microbiome ,Interleukin-10 ,Mice, Inbred C57BL ,Disease Models, Animal ,Lactobacillus ,Prebiotics ,Endocrinology ,Cytokines ,Dysbiosis ,medicine.symptom ,business ,Food Science - Abstract
The dysbiosis of gut microbiota is closely related to the occurrence and development of inflammatory bowel disease (IBD). The manipulation of intestinal flora through prebiotics or probiotics is expected to induce and maintain the remission of IBD symptoms. 6-week-old C57BL/J mice were daily gavaged with fructooligosaccharides (FOS) or the synbiotic two weeks before the administration of dextran sulfate sodium (DSS). The supplementation of FOS or synbiotic could significantly ameliorate the body weight loss and colon histological damage in DSS-induced acute colitis mice. The altered composition of gut microbiota in acute colitis mice was reversed by FOS or Synbiotic supplementation, with a characteristic of decreased abundance of Mucispirillum. Both FOS and synbiotic mitigated DSS-induced loss of mucus protein (MUC2) and epithelium tight junction proteins (ZO-1, Occluding, Claudin1) in colon mucosa. The expression of pro-inflammatory cytokines (IL-6 and TNF-α) was decreased by FOS or synbiotic treatment, while the expression of Tbx21 and IL-10 was increased. The results suggested that the modulation of gut microbiota by FOS or synbiotic supplementation could decrease the inflammation potential of colonized commensals, which prevented the impairment of the intestinal barrier and induced a regulation of immune response in DSS-induced acute colitis mice.
- Published
- 2021
12. Prognostic and clinicopathological significance of Hapto and Gremlin1 expression in extrahepatic cholangiocarcinoma
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Zhulin Yang, Xiongying Miao, Daiqiang Li, Rushi Liu, Yuan Yuan, Zhengchun Wu, and Qiong Zou
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0301 basic medicine ,medicine.medical_specialty ,Adenoma ,Gremlin1 ,Gastroenterology ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,extrahepatic cholangiocarcinoma ,medicine ,Hapto ,Stage (cooking) ,Pathological ,Survival analysis ,business.industry ,biliary tract adenoma ,medicine.disease ,stomatognathic diseases ,Exact test ,030104 developmental biology ,Oncology ,Biliary tract ,030220 oncology & carcinogenesis ,immunohistochemistry ,Immunohistochemistry ,business ,Research Paper - Abstract
Background: Some studies have demonstrated that Hapto and Gremlin1 play an important biological role in many neoplasms. However, the role of Hapto and Gremlin1 in extrahepatic cholangiocarcinoma (ECC) remains to be revealed. Thus, this study investigated the prognostic and clinicopathological significance of Hapto and Gremlin1 expression in ECC. Methods: We examined Hapto and Gremlin1 expression in 100 ECC, 30 peritumoral tissues, 10 adenoma and 15 normal biliary tract tissues using EnVision immunohistochemistry. The relationship between Hapto and Gremlin 1 expression and clinicopathological parameters was evaluated using the χ2 test or Fisher's exact test. The overall survival of patients was analyzed using Kaplan-Meier univariate survival analysis and log-rank tests. Results: Hapto and Gremlin1 proteins were overexpressed in ECC compared to peritumoral tissues, adenoma, and normal biliary tract (P
- Published
- 2020
13. Prognostic and Clinicopathological Significance of EphB3 and Dysadherin Expression in Extrahepatic Cholangiocarcinoma
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Qiong Zou, Xiong-Ying Miao, Zhengchun Wu, Zhulin Yang, Yuan Yuan, Li Xiong, Rushi Liu, and Daiqiang Li
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,Receiver operating characteristic ,Adenoma ,business.industry ,medicine.disease_cause ,medicine.disease ,Log-rank test ,stomatognathic diseases ,03 medical and health sciences ,Exact test ,030104 developmental biology ,0302 clinical medicine ,Biliary tract ,030220 oncology & carcinogenesis ,Internal medicine ,Medicine ,Immunohistochemistry ,business ,Carcinogenesis ,Survival analysis - Abstract
Aim EphB3 and dysadherin are involved in tumorigenesis and progression of many neoplasms. However, the roles of EphB3 and dysadherin in extrahepatic cholangiocarcinoma (ECC) remain to be revealed. In this study, we aimed to evaluate the expression of EphB3 and dysadherin, and investigate their clinicopathological significance in ECC. Methods We examined EphB3 and dysadherin expression in 100 ECC, 30 peritumoral tissues, 10 adenoma and 15 normal biliary tract tissues using EnVision immunohistochemistry. The relationship between EphB3 or dysadherin expression and clinicopathological features was evaluated using the χ2 test or Fisher's exact test. The overall survival of ECC patients was analyzed using Kaplan-Meier univariate survival analysis and Log rank tests. Results We found that EphB3 expression was significantly down-regulated and dysadherin expression was significantly up-regulated in ECC tissues compared with normal tissues (P < 0.01). EphB3 expression was negatively correlated with dysadherin expression in ECC (P < 0.01). The positive rate of EphB3 expression and negative rate of dysadherin expression was significantly higher in patients with well-differentiated type, no lymph node metastasis, no surrounding tissues and organs invasion, early TNM stages (I + II) and radical resection (P < 0.01). The survival of ECC patients with positive EphB3 or negative dysadherin expression was significantly longer than patients with negative EphB3 or positive dysadherin expression (P < 0.01). Cox multivariate analysis demonstrated that negative EphB3 or positive dysadherin expression were independent poor prognostic factors in ECC patients. The ROC curves suggested that EphB3 and dysadherin combined diagnostic efficacy (AUC=0.688, 95%CI: 0.603-0.772) was significantly higher EphB3 diagnostic efficacy (AUC=0.654, 95%CI: 0.564-0.743) or dysadherin diagnostic efficacy (AUC=0.648, 95%CI: 0.558-0.737) alone. Conclusion EphB3 and dysadherin are involved in the carcinogenesis and progression of ECC, and ECC patients with negative EphB3 or positive dysadherin expression have a poor prognosis.
- Published
- 2020
14. Supplementing a Specific Synbiotic Can Suppress the Incidence of AOM/DSS-Induced Colorectal Cancer in Mice
- Author
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Huixia Wu, Zhengchun Wu, Yilan Qiu, Fangjian Zhao, Minjing Liao, Zhihong Zhong, Jian Chen, Yiliang Zeng, and Rushi Liu
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
15. Expression of FOXP1 and FOXO3a in extrahepatic cholangiocarcinoma and the implications in clinicopathological significance and prognosis
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Qiong Zou, Shu Xu, Zhengchun Wu, Daiqiang Li, Zhulin Yang, Yuan Yuan, Jun He, and Lingxiang Wang
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0301 basic medicine ,medicine.medical_specialty ,Adenoma ,business.industry ,medicine.disease ,Malignancy ,Gastroenterology ,Atypical hyperplasia ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Biliary tract ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Immunohistochemistry ,Pharmacology (medical) ,business ,Lymph node ,Survival analysis - Abstract
Aims: Extrahepatic cholangiocarcinoma (EHCC) is a highly malignant tumor with poor prognosis and intrinsic resistance to cytotoxic agents. The molecular mechanisms associated with high malignancy and resistance to chemotherapy and radiotherapy have not been fully elucidated. This study investigated the clinicopathological significances of FOXP1 and FOXO3a expression in EHCC. Methods: We assayed FOXP1 and FOXO3a expressions in 100 EHCC, 30 peritumoral tissues, 10 adenoma and 15 normal biliary tract tissues using EnVision immunohistochemistry. Results: The positive rates of FOXP1 and FOXO3a proteins were significantly lower in EHCC tumors than in peritumoral tissues, adenoma, and normal bile tract tissues (P
- Published
- 2019
16. More insights into bamboo shoot degeneration
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Ali Chen, Guang-yao Yang, Zhengchun Wu, Wanqi Zhao, Jiao Xiao, and Fen Yu
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Horticulture ,Plant Science ,Degeneration (medical) ,Biology ,Bamboo shoot - Published
- 2020
17. Fructooligosaccharides supplementation mitigated chronic stress-induced intestinal barrier impairment and neuroinflammation in mice
- Author
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Liao Minjing, Zhihong Zhong, Yiliang Zeng, Hongrong Liu, Zhengchun Wu, Yuanfang Zhang, Rushi Liu, Ping Wang, Ye Xu, and Chuan Xia
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0301 basic medicine ,Serum corticosterone ,medicine.medical_specialty ,Elevated level ,Medicine (miscellaneous) ,Prebiotic ,Gut microbiota ,Gut flora ,Occludin ,digestive system ,03 medical and health sciences ,0404 agricultural biotechnology ,Neuroinflammation ,Internal medicine ,Fructooligosaccharides ,Medicine ,Chronic stress ,TX341-641 ,Brain function ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Tight junction ,biology ,business.industry ,Depression ,Nutrition. Foods and food supply ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,Endocrinology ,business ,Food Science - Abstract
Recently, accumulating evidence has suggested that gut microbiota is involved in modulating brain function and behavior through the gut-brain axis. Manipulation of gut microbiota by functional foods, such as prebiotics and probiotics, might improve mental health in stress-related psychiatric disorders. In present study, we found that supplementation of fructooligosaccharides could improve the depression-like behaviors and had a significant effect on the composition of gut flora in chronic stress mice. FOS treatment exhibited no effects on the elevated level of serum corticosterone in chronic stress mice, but significantly reversed the alterations of colonic tight junction proteins (Claudin1, Occludin, ZO-1) and reduced the pro-inflammatory cytokines (TNF-α, IL-6) in colon and brain. Thus, FOS might exert an anti-depressant effect via the prevention of intestinal barrier impairment and neuroinflammation induced by chronic stress.
- Published
- 2020
18. Increased expression of cystathionine beta-synthase and chemokine ligand 21 is closely associated with poor prognosis in extrahepatic cholangiocarcinoma
- Author
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Jun He, Daiqiang Li, Shu Xu, Yuan Yuan, Zhengchun Wu, Zhu-Lin Yang, Qiong Zou, and Lingxiang Wang
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Adult ,Male ,inorganic chemicals ,Chemokine ,congenital, hereditary, and neonatal diseases and abnormalities ,endocrine system ,Observational Study ,Kaplan-Meier Estimate ,medicine.disease_cause ,Cholangiocarcinoma ,Pathogenesis ,cystathionine beta-synthase ,03 medical and health sciences ,0302 clinical medicine ,extrahepatic cholangiocarcinoma ,medicine ,chemokine ligand 21 ,Humans ,Neoplasm Invasiveness ,030212 general & internal medicine ,Stage (cooking) ,Pathological ,Survival analysis ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Chemokine CCL21 ,biology ,business.industry ,organic chemicals ,nutritional and metabolic diseases ,General Medicine ,Middle Aged ,Cystathionine beta synthase ,Gene Expression Regulation, Neoplastic ,Bile Duct Neoplasms ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,immunohistochemistry ,Cancer research ,biology.protein ,Female ,prognosis ,Carcinogenesis ,business ,CCL21 ,Research Article - Abstract
The expression of Cystathionine beta-synthase (CBS) and Chemokine ligand 21 (CCL21) is associated with the tumorigenesis and progression of a variety of tumors, but whether alterations in their expression levels correlates with the carcinogenesis and progression of EHCC is still unknown. This study investigated the clinicopathological significance of CBS and CCL21 expression in EHCC. We investigated the correlations between the expression of CBS and CCL21 and clinicopathological characteristics in EHCC using EnVision immunohistochemistry. The expression of CBS and CCL21 was significantly higher in EHCC tumors than in nontumor tissues (P
- Published
- 2020
19. Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer
- Author
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Yuanfang Zhang, Zhulin Yang, Rushi Liu, Daiqiang Li, Xiongying Miao, Qiong Zou, Yuan Yuan, and Zhengchun Wu
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,medicine.disease_cause ,N-acetyltransferase 1 ,lcsh:RC254-282 ,gallbladder cancer ,03 medical and health sciences ,0302 clinical medicine ,medicine ,clinicopathological significance ,Gallbladder cancer ,Survival analysis ,Original Research ,business.industry ,Proportional hazards model ,Gallbladder ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,X-box-binding protein 1 ,Exact test ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Immunohistochemistry ,prognosis ,Carcinogenesis ,business - Abstract
Background: X-box-binding protein 1 (XBP1) and N-acetyltransferase 1 (NAT1) are involved in oncogenesis and progression of many human cancer types. However, the roles of XBP1 and NAT1 in gallbladder cancer (GBC) are never reported.Methods: We examined XBP1 and NAT1 expression in GBC and matched adjacent non-tumor tissues via Western blotting. Then, we assayed XBP1 and NAT1 expression in 215 GBCs, including 69 squamous cell/adenosquamous carcinomas (SC/ASCs) and 146 adenocarcinomas (ACs) with immunohistochemistry. Their prognostic and clinicopathological significance was further evaluated using the χ2 test or Fisher's exact test, Kaplan–Meier univariate survival analysis, and log-rank tests.Results: XBP1 expression was upregulated, and NAT1 expression was downregulated in GBC. Immunohistochemical results showed that XBP1 expression was negatively associated with NAT1 expression in GBC, including SC/ASC and AC. The rate of patients with an age of more than 45 years, positivity of lymph node metastasis, and invasion were significantly higher in SC/ASC than those in AC (all P < 0.05). The percentage of XBP1-positive and NAT1-negative expression was significantly higher in the cases with poor differentiation, advanced tumor, nodes, and metastases (TNM) stage, lymph node metastasis, invasion, and only receiving biopsy in GBC, SC/ASC, and AC (all P < 0.05). XBP1-positive and NAT1-negative expression was positively related to larger tumor size (>3 cm) in GBC and AC. There was a negative association between XBP1 and NAT1 expression in GBC, SC/ASC, and AC (all P < 0.05). Positive XBP1 and negative NAT1 expression was closely associated with decreased overall survival in GBC, SC/ASC, and AC patients (all P < 0.05). The multivariate Cox regression analysis showed that positive XBP1 or negative NAT1 expression was an independent factor for poor prognosis in gallbladder SC/ASC and AC patients.Conclusions: This study indicates that positive XBP1 and negative NAT1 expression are closely associated with the clinicopathological and biological behaviors and poor prognosis in GBC.
- Published
- 2020
20. Expression of UGP2 and CFL1 expression levels in benign and malignant pancreatic lesions and their clinicopathological significance
- Author
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Ziru Liu, Zhengchun Wu, Qiong Zou, Zhulin Yang, Lingxiang Wang, Daiqiang Li, Li Xiong, and Xiongying Miao
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0301 basic medicine ,Cofilin 1 ,Male ,Pathology ,medicine.medical_specialty ,UTP-Glucose-1-Phosphate Uridylyltransferase ,UGP2 ,Pancreatic Intraepithelial Neoplasia ,lcsh:Surgery ,Adenocarcinoma ,lcsh:RC254-282 ,Atypical hyperplasia ,Pancreatic intraepithelial neoplasia ,Pancreatic ductal adenocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,Pancreatic cancer ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasm Invasiveness ,Survival rate ,Pancreas ,Survival analysis ,CFL1 ,Intraepithelial neoplasia ,business.industry ,Research ,lcsh:RD1-811 ,Middle Aged ,medicine.disease ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Pancreatic Neoplasms ,Survival Rate ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Case-Control Studies ,Lymphatic Metastasis ,Surgery ,Female ,business ,Chronic pancreatitis ,Carcinoma, Pancreatic Ductal ,Follow-Up Studies - Abstract
Background This study investigated UGP2 (uridine diphosphate-glucose pyrophosphorylase-2) and CFL1 (cofilin-1) expression in pancreatic ductal carcinoma (PDC), paracancerous tissue (PT), benign lesions (BL), and normal tissue (NT) and their clinicopathological significance. Methods Surgical specimens, which were collected from 106 cases of pancreatic ductal carcinoma, 35 cases of paracancerous tissues, 55 cases of benign lesions and 13 cases of normal pancreatic tissues, were fixed with 4% formaldehyde to prepare conventional paraffin-embedded sections. EnVision immunohistochemical was used to stain for UGP2 and CFL1. Kaplan-Meier survival analysis was performed to assess the correlation of expression pattern with survival. Results We found that positive UGP2 and CFL1 expression in PDC were significantly higher than those in PT, BL, and NT. In PT and BL with positive UGP2 and CFL1 expression, mild to severe atypical hyperplasia or intraepithelial neoplasia of grades II–III was observed in ductal epithelium. Positive UGP2 and CFL1 expression in cases with high differentiation, no lymph node metastasis, no surrounding invasion, and TNM (tumor-node-metastasis) staging I or/and II were significantly lower than those in cases with poor differentiation, lymph node metastasis, surrounding invasion, and TNM stage III and/or IV. Positive UGP2 expression in male patients was significantly lower than that in female patients. UGP2 and CFL1 expression in PDC were positively correlated. Kaplan-Meier survival analysis showed the degree of differentiation, tumor maximal diameter, TNM stage, lymph node metastasis, and surrounding invasion, and UGP2 and CFL1 expression were closely related to the average survival time of patients with PDC. The survival time of patients with positive UGP2 and CFL1 expression was significantly shorter than that of patients with negative expression. Cox multivariate analysis showed that poor differentiation, tumor maximal diameter ≥ 3 cm, TNM stage III or IV, lymph node metastasis, surrounding invasion, and positive UGP2 and CFL1 expression was negatively correlated with the postoperative survival rate and positively correlated with the mortality of patients with PDC. Conclusion Positive expression of UGP2 and CFL1 can serve a valuable prognostic factor in pancreatic cancer.
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- 2018
21. Increased expression of cystathionine beta-synthase and chemokine ligand 21 is closely associated with poor prognosis in extrahepatic cholangiocarcinoma.
- Author
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Lingxiang Wang, Zhulin Yang, Zhengchun Wu, Jun He, Shu Xu, Daiqiang Li, Qiong Zou, Yuan Yuan, Wang, Lingxiang, Yang, Zhulin, Wu, Zhengchun, He, Jun, Xu, Shu, Li, Daiqiang, Zou, Qiong, and Yuan, Yuan
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- 2020
- Full Text
- View/download PDF
22. Comparative study of ROR2 and WNT5a expression in squamous/adenosquamous carcinoma and adenocarcinoma of the gallbladder
- Author
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Lingxiang Wang, Ziru Liu, Qiong Zou, Zhulin Yang, Li Xiong, Zhengchun Wu, Hua Zhao, Xiongying Miao, Kuijie Liu, and Daiqiang Li
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Male ,0301 basic medicine ,Oncology ,Pathology ,Time Factors ,Clinical Trials Study ,Kaplan-Meier Estimate ,0302 clinical medicine ,Risk Factors ,Gallbladder adenosquamous carcinoma ,WNT5a ,ROR2 ,Aged, 80 and over ,Gallbladder Adenosquamous Carcinoma ,Gallbladder adenocarcinoma ,Gastroenterology ,Gallbladder squamous carcinoma ,Cell Differentiation ,General Medicine ,Middle Aged ,Immunohistochemistry ,Up-Regulation ,WNT5A ,Treatment Outcome ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,embryonic structures ,Adenocarcinoma ,Female ,Gallbladder Neoplasms ,Adult ,China ,medicine.medical_specialty ,Adenosquamous carcinoma ,Receptor Tyrosine Kinase-like Orphan Receptors ,Wnt-5a Protein ,Carcinoma, Adenosquamous ,03 medical and health sciences ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Chi-Square Distribution ,business.industry ,Gallbladder ,medicine.disease ,digestive system diseases ,body regions ,stomatognathic diseases ,030104 developmental biology ,Multivariate Analysis ,sense organs ,Gallbladder Neoplasm ,business - Abstract
AIM To investigate the expression and clinical pathological significance of ROR2 and WNT5a in gallbladder squamous/adenosquamous carcinoma (SC/ASC) and adenocarcinoma (AC). METHODS EnVision immunohistochemistry was used to stain for ROR2 and WNT5a in 46 SC/ASC patients and 80 AC patients. RESULTS Poorly differentiated AC among AC patients aged > 45 years were significantly more frequent compared with SC/ASC patients, while tumors with a maximal diameter > 3 cm in the SC/ASC group were significantly more frequent compared with the AC group. Positive ROR2 and WNT5a expression was significantly lower in SC/ASC or AC with a maximal mass diameter ≤ 3 cm, a TNM stage of I + II, no lymph node metastasis, no surrounding invasion, and radical resection than in patients with a maximal mass diameter > 3 cm, TNM stage IV, lymph node metastasis, surrounding invasion, and no resection. Positive ROR2 expression in patients with highly differentiated SC/ASC was significantly lower than in patients with poorly differentiated SC/ASC. Positive ROR2 and WNT5a expression levels in highly differentiated AC were significantly lower than in poorly differentiated AC. Kaplan-Meier survival analysis showed that differentiation degree, maximal mass diameter, TNM stage, lymph node metastasis, surrounding invasion, surgical procedure and the ROR2 and WNT5a expression levels were closely related to average survival of SC/ASC or AC. The survival of SC/ASC or AC patients with positive expression of ROR2 and WNT5a was significantly shorter than that of patients with negative expression results. Cox multivariate analysis revealed that poor differentiation, a maximal diameter of the mass ≥ 3 cm, TNM stage III or IV, lymph node metastasis, surrounding invasion, unresected surgery and positive ROR2 or WNT5a expression in the SC/ASC or AC patients were negatively correlated with the postoperative survival rate and positively correlated with mortality, which are risk factors and independent prognostic predictors. CONCLUSION SC/ASC or AC patients with positive ROR2 or WNT5a expression generally have a poor prognosis.
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- 2017
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