34 results on '"Zhexun Lian"'
Search Results
2. Interfacing exogenous stents with human coronary artery by self-assembled coating: designs, functionalities and applications
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Feng Zhao, Feng Liu, Chenglong Gao, Guoqing Wang, Yinfeng Zhang, Fei Yu, Jiawei Tian, Kai Tan, Runhao Zhang, Kang Liang, Zhexun Lian, Junjie Guo, Biao Kong, Junbo Ge, and Hui Xin
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Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Drug-eluting stents are a commonly used treatment for coronary artery disease. However, the coatings used in drug-eluting stents have some limitations such as poor biocompatibility and drug loading capacity. In recent years, self-assembly methods have emerged as a promising alternative for stent coatings. Self-assembled coatings employ biomaterials and offer several advantages over traditional coatings, including thinner thickness, stronger binding capacity, and better biocompatibility. This review discusses the latest research on self-assembled biomaterial-based coatings for drug-eluting stents. We explore how layer-by-layer coatings and composite coating films have been utilized to load and release drugs with high drug loading capacity and biocompatibility, as well as how they promote endothelial adhesion and growth. Additionally, we examine how self-assembled coatings have been used to release active molecules for anti-coagulation and deliver gene therapy. Moreover, we discuss the potential of self-assembled coatings for future development, including intelligent targeted drug delivery, bionic stent coatings, and 3D printed stent coatings. These advancements have the potential to further improve the effectiveness of drug-eluting stents in treating coronary artery disease.
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- 2024
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3. Comparison of electrocardiogram parameters and echocardiographic response between distinct left bundle branch area pacing modes in heart failure patients
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Yao Li, Wei Zhang, Keping Chen, and Zhexun Lian
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left bundle branch area pacing ,branch pacing ,electrocardiogram ,echocardiographic response ,heart failure ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundLeft bundle branch area pacing (LBBAP) has become an alternative method for cardiac resynchronization therapy. Various modes of LBBAP have been determined, including left bundle trunk pacing (LBTP), left anterior branch pacing (LAFP) and left posterior branch pacing (LPFP). However, whether the outcomes of various pacing modes differ in heart failure (HF) patients is still unclear. This study aimed to compare the electrophysiological characteristics and echocardiographic response rate among those distinct modes of LBBAP.MethodsHF patients undergoing successful LBBAP were retrospectively included. Distinct modes of pacing were determined based on paced QRS morphology. The fluoroscopic images were collected to compare the lead tip position between the groups. The electrocardiograms (ECG) before and after LBBAP were used to measure the depolarization (QRS duration [QRSd] and the interventricular delay [IVD]), and the repolarization parameters [QTc, TpeakTend(TpTe), and TpTe/QTc]. The left ventricular ejection fraction (LVEF) and left ventricular end-diastolic diameter (LVEDD) of patients were also recorded. In addition, the lead parameters and certain complications were compared.ResultsA total of 64 HF patients were finally included, consisting of 16 (25.0%) patients in the LBTP group, 22 (34.4%) patients in the LAFP group, and 26 (40.6%) patients in the LPFP group. The distribution features of LBBAP lead tips were significantly related to pacing modes: LBTP was more likely to be in zone 4 while LAFP or LPFP was prone to locate in zone 5. After LBBAP, the ventricular ECG parameters were significantly improved, regardless of pacing modes. Besides, the LVEF of the patients was significantly increased (P 0.05). In addition, the lead parameters remained stable and no significant difference was observed among groups.ConclusionLPFP was the main pacing mode among HF patients after LBBAP. The paced QRS morphology was significantly related to the position of lead tips. After LBBAP, the ventricular depolarization synchronization and repolarization stability were both significantly improved, regardless of pacing modes. There was no significant difference in the echocardiographic response rate among distinct LBBAP modes.
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- 2024
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4. Prevalence and mortality prognosis of steatotic liver disease phenotypes
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Zhiyu Sun, Chi Zhou, Yiwen Zhang, Pengfei Li, Junjie Guo, Zhexun Lian, and Hongwei Ji
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Specialties of internal medicine ,RC581-951 - Published
- 2024
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5. The TFPI2–PPARγ axis induces M2 polarization and inhibits fibroblast activation to promote recovery from post-myocardial infarction in diabetic mice
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Mengqi Guo, Zongyi Xia, Yefeng Hong, Hongwei Ji, Fuhai Li, Wenheng Liu, Shaohua Li, Hui Xin, Kai Tan, and Zhexun Lian
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Tissue factor pathway inhibitor-2 ,Diabetes mellitus ,Myocardial infarction ,Fibroblast migration ,MMPs ,Collagen ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract Background Diabetes mellitus is one of the causes of poor ventricular remodelling and poor cardiac recovery after myocardial infarction (MI). We previously reported that tissue factor pathway inhibitor-2 (TFPI2) was downregulated in response to hyperglycaemia and that it played a pivotal role in extracellular matrix (ECM) degradation and cell migration. Nonetheless, the function and mechanism of TFPI2 in post-MI remodelling under diabetic conditions remain unclear. Therefore, in the present study, we investigated the role of TFPI2 in post-MI effects in a diabetic mouse model. Results TFPI2 expression was markedly decreased in the infarcted myocardium of diabetic MI mice compared with that in non-diabetic mice. TFPI2 knockdown in the MI mouse model promoted fibroblast activation and migration as well as matrix metalloproteinase (MMP) expression, leading to disproportionate fibrosis remodelling and poor cardiac recovery. TFPI2 silencing promoted pro-inflammatory M1 macrophage polarization, which is consistent with the results of TFPI2 downregulation and M1 polarization under diabetic conditions. In contrast, TFPI2 overexpression in diabetic MI mice protected against adverse cardiac remodelling and functional deterioration. TFPI2 overexpression also inhibited MMP2 and MMP9 expression and attenuated fibroblast activation and migration, as well as excessive collagen production, in the infarcted myocardium of diabetic mice. TFPI2 promoted an earlier phenotype transition of pro-inflammatory M1 macrophages to reparative M2 macrophages via activation of peroxisome proliferator-activated receptor gamma. Conclusions This study highlights TFPI2 as a promising therapeutic target for early resolution of post-MI inflammation and disproportionate ECM remodelling under diabetic conditions.
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- 2023
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6. Protective effect of ischaemic postconditioning combined with nicorandil on myocardial ischaemia‒reperfusion injury in diabetic rats
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Zongyi Xia, Bing Chen, Chi Zhou, Yitian Wang, Jinyang Ren, Xujin Yao, Yifan Yang, Qi Wan, and Zhexun Lian
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Ischaemia postconditioning ,Myocardial ischaemia‒reperfusion injury ,Nicorandil ,PI3K/Akt signalling pathway ,Type 2 diabetes mellitus ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background The diabetic heart exhibits a high sensitivity to ischaemia/reperfusion (I/R) injury. Diabetes mellitus (DM) can affect the efficacy of cardioprotective interventions and reduce the therapeutic potential of existing treatment options. This study aimed to investigate the feasibility of shifting from monotherapy to combination therapy in diabetic myocardial I/R injury. Methods 6–8 week rats were randomized into 10 groups: sham, I/R, ischaemia postconditioning (I-Post), nicorandil (Nic), combination therapy (I-Post + Nic), DM sham, DM I/R, DM I-Post, DM Nic and DM I-Post + Nic. The extent of myocardial injury was clarified by measuring CK-MB and NO levels in plasma, ROS content in myocardial tissues, and TTC/Evans Blue staining to assess the area of myocardial infarction. Pathological staining of cardiac tissue sections were performed to clarify the structural changes in myocardial histopathology. Finally, Western blotting was performed to detect the phosphorylation levels of some key proteins in the PI3K/Akt signalling pathway in myocardial tissues. Results We confirms that myocardial injury in diabetic I/R rats remained at a high level after treatment with I-Post or nicorandil alone. I-Post combined with nicorandil showed better therapeutic effects in diabetic I/R rats, and the combined treatment further reduced the area of myocardial injury in diabetic I/R rats compared with I-Post or nicorandil treatment alone (P
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- 2022
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7. Transcatheter versus surgical aortic valve replacement in patients with aortic regurgitation: A propensity-matched analysis
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Chi Zhou, Zongyi Xia, Yanxu Song, and Zhexun Lian
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Aortic regurgitation ,Transcatheter aortic valve replacement ,Surgical aortic valve replacement ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
This study aimed to analyze in-hospital and early-to-interim outcomes of pure aortic regurgitation (AR) using transcatheter aortic valve replacement (TAVR) vs. surgical aortic valve replacement (SAVR). Background: Few studies have discussed and compared the safety and short-term prognosis of TAVR and SAVR in pure AR patients. As such, we looked to the National Readmissions Database (NRD) for records between 2016 and 2019 in order to identify patients diagnosed with pure AR who underwent SAVR or TAVR. We used the propensity score matching to minimize disparities between two groups. We included 23,276 pure AR patients: 1983 (8.5%) who underwent TAVR and 21,293 (91.5%) who underwent SAVR. We found 1820 matched pairs using propensity score matching. In the matching cohort, TAVR was associated with a low risk of in-hospital mortality. Although TAVR had lower incidences of 30-day all-cause readmission (hazard ratio (HR):0.73, 95% confidence interval (CI): 0.61–0.87; P
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- 2023
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8. Association of age at menarche with valvular heart disease: An analysis based on electronic health record (CREAT2109)
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Zhiyu Sun, Yongjie Zhu, Xiaoyan Sun, Zhexun Lian, Mengqi Guo, Xiaohong Lu, Ting Song, Luxin Feng, Yi Zhang, Yawei Xu, Hongwei Ji, and Junjie Guo
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age at menarche ,reproductive history ,heart valve diseases ,electronic health records ,risk factors ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundThe association between age at menarche and coronary heart disease has been reported, but the association between age at menarche and valvular heart disease (VHD) has not been described. We aimed to examine the association between age at menarche and VHD.MethodsBy collecting data from four medical centers of the Affiliated Hospital of Qingdao University (QUAH) from January 1, 2016, to December 31, 2020, we sampled 105,707 inpatients. The main outcome of this study was newly diagnosed VHD, which was diagnosed based on ICD-10 coding, and the exposure factor was age at menarche, which was accessed through the electronic health records. We used logistic regression model to investigate the association between age at menarche and VHD.ResultsIn this sample (mean age 55.31 ± 13.63 years), the mean age at menarche was 15. Compared with women with age at menarche 14–15 years, the odds ratio of VHD in women with age at menarche ≤13, 16–17, and ≥18 years was 0.68 (95% CI 0.57–0.81), 1.22 (95% CI 1.08–1.38), and 1.31 (95% CI 1.13–1.52), respectively (P for all
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- 2023
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9. Temporal Trends and Early Outcomes of Transcatheter versus Surgical Mitral Valve Repair in Atrial Fibrillation Patients
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Chi Zhou, Kai Tan, Weili Liu, Shaohua Li, Zongyi Xia, Yanxu Song, and Zhexun Lian
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Objectives. To study trends of utilization, in-hospital outcomes, and short outcomes in patients undergoing transcatheter mitral valve repair (TMVR) vs. surgical mitral valve repair (SMVR) in atrial fibrillation (AF). Background. TMVR is a treatment option in inoperable or high-risk patients with mitral regurgitation (MR). AF is a common comorbidity of MR. Data comparing between TMVR and SMVR in MR patients with AF is lacking. Methods. The National Readmission Database from 2016 to 2019 was utilized to identify hospitalizations undergoing TMVR or SMVR with AF. Outcomes of interest included mortality, postoperative complications, length of stay, and 30-day readmission rate. Results. A total of 9,195 patients underwent TMVR and 16,972 patients underwent SMVR with AF; the number of AF undergoing TMVR was increasing from 1,342 in 2016 to 4,215 in 2019 and SMVR. The incidence of in-hospital mortality decreased from 2.6% in 2016 to 1.8% in 2019. We identified length of stay>5 days, dyslipidemia, cerebrovascular disease, heart failure with reduced ejection fraction, and urgent/emergent admissions as independent risk factors for in-hospital mortality. After matching, we included 4,680 patients in each group; the in-hospital death, transfusion, acute kidney injury, sepsis, stroke, and mechanical ventilation were lower in TMVR compared with SMVR. TMVR was associated with a similar rate of all-cause readmission at 30 days compared with SMVR. Conclusion. Patients with AF receiving TMVR have been increasing along with progressive improvement in in-hospital death and length of stay. Compared to SMVR, AF patients receiving TMVR had a lower rate of in-hospital death and postoperative complications.
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- 2023
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10. Increased ratio of sST2/LVMI predicted cardiovascular mortality and heart failure rehospitalization in heart failure with reduced ejection fraction patients: a prospective cohort study
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Fuhai Li, Mengying Xu, Mingqiang Fu, Xiaotong Cui, Zhexun Lian, Hui Xin, Jingmin Zhou, and Junbo Ge
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Soluble suppression of tumorigenicity 2 ,Left ventricular mass index ,Heart failure ,Cardiac remodeling ,Heart failure with reduced ejection fraction ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Inflammation is one of the principal triggering mechanisms for left ventricular fibrosis and remodeling in heart failure, leading to adverse clinical outcomes. Soluble suppression of tumorigenicity 2 (sST2), a member of the interleukin-1 receptor family, is assumed to play a significant role in the fibrotic response to inflammation. Left ventricular mass index (LVMI) is a parameter of the prefibrotic inflammatory phase of heart failure preceding remodeling. The present study aimed to investigate the prognostic value of the sST2/LVMI ratio in heart failure with reduced ejection fraction. Methods This was a prospective cohort study. A total of 45 consecutive patients with heart failure with reduced ejection fraction, treated between September 2015 and December 2016, were enrolled. The sST2/LVMI ratio was measured at baseline. The primary endpoint was a composite of cardiovascular mortality and readmission for heart failure. The prognostic impact of the sST2/LVMI ratio was evaluated using a multivariable Cox proportional hazards regression model. Results Forty-five patients were enrolled in this study. Their average age was 48 ± 14 years, and approximately 20% of them were men. Patients were followed for 9 months, during which the primary outcome occurred in 15 patients. Kaplan–Meier analysis showed that patients with a high sST2/LVMI ratio (≥ 0.39) had shorter event-free survival than those with intermediate (between 0.39 and 0.24) and low ratios (
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- 2021
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11. Long Non-coding RNA PEBP1P2 Suppresses Proliferative VSMCs Phenotypic Switching and Proliferation in Atherosclerosis
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Xingqiang He, Zhexun Lian, Yanyan Yang, Zhibin Wang, Xiuxiu Fu, Yan Liu, Min Li, Jiawei Tian, Tao Yu, and Hui Xin
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cardiovascular diseases ,vascular smooth muscle cells ,long non-coding RNAs ,phenotypic switching ,CDK9 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Long non-coding RNAs (lncRNAs) play a crucial role in the growth of vascular smooth muscle cells (VSMCs), the dysfunction of which is closely associated with the initiation and progression of cardiovascular diseases (CVDs). Abnormal phenotypic switching and proliferation of VSMCs constitute a significant event in the progression of atherosclerosis. The present study identified a novel lncRNA, PEBP1P2, which serves as a valuable regulator of VSMCs in phenotypic transformation and proliferation. The expression of PEBP1P2 was remarkably decreased in proliferating VSMCs and pathological arteries when using a balloon injury model of rats. Furthermore, we found that PEBP1P2 represses proliferation, migration, and dedifferentiation during phenotype switching in VSMCs induced by platelet-derived growth factor BB (PDGF-BB). Mechanistically, cyclin-dependent kinase 9 (CDK9) was confirmed to be the direct target of PEBP1P2, which was proven to mediate phenotypic switching and proliferation of VSMCs and was rescued by PEBP1P2. Then, we explored the clinical significance, as we observed the decreased expression of PEBP1P2 in the serum of coronary heart disease (CHD) patients and human advanced carotid atherosclerotic plaques. Finally, PEBP1P2 overexpression distinctly suppressed neointima formation and VSMC phenotypic switching in vivo. Taken together, PEBP1P2 inhibits proliferation and migration in VSMCs by directly binding to CDK9, implying that it may be a promising therapeutic target for the treatment of proliferative vascular diseases.
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- 2020
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12. Prediction model of in-hospital mortality in intensive care unit patients with heart failure: machine learning-based, retrospective analysis of the MIMIC-III database
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Mingqiang Fu, Jingmin Zhou, Jidong Zhang, Fuhai Li, Hui Xin, and Zhexun Lian
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Medicine - Abstract
Objective The predictors of in-hospital mortality for intensive care units (ICUs)-admitted heart failure (HF) patients remain poorly characterised. We aimed to develop and validate a prediction model for all-cause in-hospital mortality among ICU-admitted HF patients.Design A retrospective cohort study.Setting and participants Data were extracted from the Medical Information Mart for Intensive Care (MIMIC-III) database. Data on 1177 heart failure patients were analysed.Methods Patients meeting the inclusion criteria were identified from the MIMIC-III database and randomly divided into derivation (n=825, 70%) and a validation (n=352, 30%) group. Independent risk factors for in-hospital mortality were screened using the extreme gradient boosting (XGBoost) and the least absolute shrinkage and selection operator (LASSO) regression models in the derivation sample. Multivariate logistic regression analysis was used to build prediction models in derivation group, and then validated in validation cohort. Discrimination, calibration and clinical usefulness of the predicting model were assessed using the C-index, calibration plot and decision curve analysis. After pairwise comparison, the best performing model was chosen to build a nomogram according to the regression coefficients.Results Among the 1177 admissions, in-hospital mortality was 13.52%. In both groups, the XGBoost, LASSO regression and Get With the Guidelines-Heart Failure (GWTG-HF) risk score models showed acceptable discrimination. The XGBoost and LASSO regression models also showed good calibration. In pairwise comparison, the prediction effectiveness was higher with the XGBoost and LASSO regression models than with the GWTG-HF risk score model (p
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- 2021
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13. Gender Differences in Age-Stratified Early Outcomes in Patients With Transcatheter Aortic Valve Implantation
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Chi, Zhou, Zongyi, Xia, Bing, Chen, Yanxu, Song, and Zhexun, Lian
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Adult ,Male ,Aged, 80 and over ,Heart Valve Prosthesis Implantation ,Adolescent ,Aortic Valve Stenosis ,Middle Aged ,Transcatheter Aortic Valve Replacement ,Young Adult ,Sex Factors ,Treatment Outcome ,Risk Factors ,Aortic Valve ,Humans ,Female ,Hospital Mortality ,Cardiology and Cardiovascular Medicine ,Aged - Abstract
Few researchers have discussed the differences in gender between the age groups of patients who underwent transcatheter aortic valve implantation (TAVI). We searched the National Readmissions Database from 2012 to 2019 to identify adults who underwent TAVI. We studied hospital outcomes and short- to medium-term outcomes by age stratification (18 to 59, 60 to 69, 70 to 79, and 80 to 90 years) after TAVI and categorized by gender. We included 147,481 patients who underwent TAVI, and 54,802 pairs were matched using propensity score matching separately for each age group. Compared with men, women in all age groups had a similar rate of hospital death. Except the 18- to 59-year-old groups, female patients were less likely to undergo permanent pacemaker implantation and transfusion. Records of readmission at 30 days and 6 months were used as the follow-up outcome according to the presence or absence of readmission. Major adverse cardiovascular events (MACEs) were a composite of cardiovascular readmission, all-cause mortality during readmission, and stroke readmission. At the 30-day follow-up visit, there was no difference in the all-cause readmission and MACE between women and men in any group. At the 6-month follow-up visit, women in the 70- to 79-year-old and 80- to 90-year-old groups had a high risk of all-cause readmission. In conclusion, we reported that female patients have similar in-hospital death rates to male patients who underwent TAVI. During the 30-day follow-up visit, the all-cause readmission and MACE were not different in all age groups between men and women. At 6 months, women in the 70- to 79-year-old and 80- to 90-year-old groups had a higher risk of all-cause readmission.
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- 2023
14. Increased ratio of sST2/LVMI predicted cardiovascular mortality and heart failure rehospitalization in heart failure with reduced ejection fraction patients: a prospective cohort study
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Zhexun Lian, Mingqiang Fu, Jingmin Zhou, Xiaotong Cui, Junbo Ge, Mengying Xu, Fuhai Li, and Hui Xin
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,Diastole ,Heart failure ,Patient Readmission ,Risk Assessment ,Ventricular Function, Left ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,medicine ,Humans ,Diseases of the circulatory (Cardiovascular) system ,Prospective Studies ,Interventricular septum ,Prospective cohort study ,Soluble suppression of tumorigenicity 2 ,Aged ,Cardiac remodeling ,Ejection fraction ,Ventricular Remodeling ,Proportional hazards model ,business.industry ,Hazard ratio ,Stroke Volume ,Middle Aged ,medicine.disease ,Heart failure with reduced ejection fraction ,Interleukin-1 Receptor-Like 1 Protein ,Magnetic Resonance Imaging ,Progression-Free Survival ,Cardiac surgery ,medicine.anatomical_structure ,Echocardiography ,RC666-701 ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers ,Left ventricular mass index ,Research Article ,Heart Failure, Systolic - Abstract
Background Inflammation is one of the principal triggering mechanisms for left ventricular fibrosis and remodeling in heart failure, leading to adverse clinical outcomes. Soluble suppression of tumorigenicity 2 (sST2), a member of the interleukin-1 receptor family, is assumed to play a significant role in the fibrotic response to inflammation. Left ventricular mass index (LVMI) is a parameter of the prefibrotic inflammatory phase of heart failure preceding remodeling. The present study aimed to investigate the prognostic value of the sST2/LVMI ratio in heart failure with reduced ejection fraction. Methods This was a prospective cohort study. A total of 45 consecutive patients with heart failure with reduced ejection fraction, treated between September 2015 and December 2016, were enrolled. The sST2/LVMI ratio was measured at baseline. The primary endpoint was a composite of cardiovascular mortality and readmission for heart failure. The prognostic impact of the sST2/LVMI ratio was evaluated using a multivariable Cox proportional hazards regression model. Results Forty-five patients were enrolled in this study. Their average age was 48 ± 14 years, and approximately 20% of them were men. Patients were followed for 9 months, during which the primary outcome occurred in 15 patients. Kaplan–Meier analysis showed that patients with a high sST2/LVMI ratio (≥ 0.39) had shorter event-free survival than those with intermediate (between 0.39 and 0.24) and low ratios (P = 0.022). The fully adjusted multivariable Cox regression analysis showed that the sST2/LVMI ratio was positively associated with the composite outcome in patients with heart failure with reduced ejection fraction after adjusting for confounders (hazard ratio 1.64, 95% confidence interval 1.06 to 2.54). By subgroup analysis, a stronger association was found with age between 40 and 55 years, systolic blood pressure Conclusion In patients with heart failure with reduced ejection fraction, the relationship between the sST2/LVMI ratio and the composite outcome was linear. A higher baseline ratio of sST2/LVMI was associated with an increased risk of cardiovascular mortality and heart failure rehospitalization in the short-term follow-up.
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- 2021
15. Front Cover: Superassembled Hierarchical Asymmetric Magnetic Mesoporous Nanorobots Driven by Smart Confined Catalytic Degradation (Chem. Eur. J. 29/2022)
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Miao Yan, Ding Ma, Beilei Qiu, Tianyi Liu, Lei Xie, Jie Zeng, Kang Liang, Hui Xin, Zhexun Lian, Lei Jiang, and Biao Kong
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Organic Chemistry ,General Chemistry ,Catalysis - Published
- 2022
16. Effect of home exercise rehabilitation on cardiopulmonary function in patients after PCI: a retrospective cohort study
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Jidong Zhang, LI Fuhai, Yan Li, Lihua Cao, Chunli Cui, Wenzhong Zhang, and Zhexun Lian
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Objectives This study aimed to compare the effects of different durations of home exercise rehabilitation on left ventricular remodeling and cardiopulmonary function in patients with acute coronary syndrome (ACS) treated with percutaneous coronary intervention (PCI). Methods All patients received individualized home exercise rehabilitation guidance and treatment after discharge from the hospital. Echocardiography and CPET were performed before and after the rehabilitation intervention. The echocardiographic parameters and CPET results of the two groups were compared before and after rehabilitation. Differences between the groups were compared for each parameter. Results Forty patients were enrolled. Patients were divided into long-term home rehabilitation group (6–12 months, n = 18) and short-term home rehabilitation group (3 to n = 22).The CPET parameters ATVO2, peak VO2/kg, and peak METs in the long-term home rehabilitation group increased after rehabilitation compared with those before rehabilitation (P 2 slope, which was not statistically significant (P > 0.05), ATVO2, peak VO2/HR, peak VO2/kg, peak METs, and peak power all increased in the long-term home rehabilitation group compared with the short-term home rehabilitation group(P P 2 and peak power (r = 0.7156828,P = 0.009 and r = 0.457907,P = 0.034). Conclusion Long-term home rehabilitation of patients with ACS after PCI improved the cardiopulmonary function and exercise capacity.
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- 2022
17. Superassembled Hierarchical Asymmetric Magnetic Mesoporous Nanorobots Driven by Smart Confined Catalytic Degradation
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Miao Yan, Ding Ma, Beilei Qiu, Tianyi Liu, Lei Xie, Jie Zeng, Kang Liang, Hui Xin, Zhexun Lian, Lei Jiang, and Biao Kong
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Physical Phenomena ,Magnetic Phenomena ,Organic Chemistry ,Humans ,Metal Nanoparticles ,General Chemistry ,Gold ,Silicon Dioxide ,Catalysis - Abstract
Micro/nanoscale robotics has received great attention in many important fields. However, it is still a great challenge to construct nanorobots simultaneously possessing multifunctionality, well-controlled directionality, and fast and durable motion as well as fully compatible and biodegradable components. Here, a hierarchical, asymmetric, hollow, catalytic, magnetic, and mesoporous nanorobot has been fabricated through a multistep interfacial superassembly strategy. The multilayer composites consist of hollow silica nanoflasks sequentially coated with a highly magnetic responsive Fe
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- 2022
18. Biointerface topography regulates phenotypic switching and cell apoptosis in vascular smooth muscle cells
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Qihui Zhou, Yan Liu, Tao Yu, Yanyan Yang, Zhexun Lian, Mingzhe Yan, Qingde Yin, Liangliang Yang, Lu Han, Patrick van Rijn, Min Li, Photophysics and OptoElectronics, Nanotechnology and Biophysics in Medicine (NANOBIOMED), and Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
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0301 basic medicine ,In-stent restenosis ,Phenotypic switching ,Vascular smooth muscle ,Phalloidin ,Surface Properties ,Biophysics ,BIOLOGY ,Caspase 3 ,Apoptosis ,Microscopy, Atomic Force ,Biochemistry ,STENT RESTENOSIS ,Muscle, Smooth, Vascular ,Cell Line ,Major Histocompatibility Complex ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Vascular smooth muscle cells ,Humans ,Dimethylpolysiloxanes ,OXIDATIVE STRESS ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,Polydimethylsiloxane ,Tissue Scaffolds ,Cell growth ,Calcium-Binding Proteins ,Microfilament Proteins ,Cell Biology ,Cell sorting ,Actins ,Cell biology ,Biomechanical Phenomena ,Blot ,Calponin 1 ,Wrinkled topography ,030104 developmental biology ,Cross-Linking Reagents ,Phenotype ,chemistry ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Tumor Suppressor Protein p53 - Abstract
Background In-stent restenosis (ISR) is a complex disease that occurs after coronary stenting procedures. The development of quality materials and improvement of our understanding on significant factors regulating ISR are essential for enhancing prognosis. Vascular smooth muscle cells (VSMCs) are the main constituent cells of blood vessel walls, and dysfunction of VMSCs can exacerbate ISR. Accordingly, in this study, we explored the influence of wrinkled material topography on the biological functions of VSMCs. Methods Polydimethylsiloxane with a wrinkled topography was synthesized using elastomer base and crosslinking and observed by atomic force microscopy. VSMC proliferation, apoptosis, and morphology were determined by Cell Counting Kit-8 assays, fluorescence-assisted cell sorting, and phalloidin staining. α-Smooth muscle actin (α-SMA), major histocompatibility complex (MHC), and calponin 1 (CNN-1) expression levels were measured by quantitative real-time polymerase chain reaction and western blotting. Moreover, p53 and cleaved caspase-3 expression levels were evaluated by western blotting in VSMCs to assess apoptotic induction. Results Surface topographies were not associated with a clear orientation or elongation of VSMCs. The number of cells was increased on wrinkled surfaces (0.7 μm in amplitude, and 3 μm in wavelength [W3]) compared with that on other surfaces, contributing to continuously increased cell proliferation. Moreover, interactions of VSMCs with the W3 surface suppressed phenotypic switching, resulting in ISR via regulation of α-SMA, calponin-1, and SM-MHC expression. The surface with an amplitude of 0.05 μm and a wavelength of 0.5 μm (W0.5) promoted apoptosis by inducing caspase 3 and p53 activities. Conclusion Introduction of aligned topographies on biomaterial scaffolds could provide physical cues to modulate VSMC responses for engineering vascular constructs. Materials with wrinkled topographies could have applications in the development of stents to reduce ISR.
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- 2020
19. TRIM25 Rescues Against Doxorubicin-Induced Pyroptosis Through Promoting NLRP1 Ubiquitination
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Xiaxia Wang, Kai Tan, Zhexun Lian, Shan Li, Dongqiang Yu, and Yiping Ge
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TRIM25 ,Time Factors ,Heart Diseases ,Nerve Tissue Proteins ,macromolecular substances ,Toxicology ,Cell Line ,Ubiquitin ,polycyclic compounds ,medicine ,Pyroptosis ,Animals ,Doxorubicin ,Myocytes, Cardiac ,Rats, Wistar ,Molecular Biology ,Cardiotoxicity ,TUNEL assay ,Antibiotics, Antineoplastic ,biology ,Chemistry ,organic chemicals ,technology, industry, and agriculture ,Ubiquitination ,In vitro ,Cell biology ,Ubiquitin ligase ,Rats ,carbohydrates (lipids) ,DNA-Binding Proteins ,Disease Models, Animal ,biology.protein ,Cardiology and Cardiovascular Medicine ,medicine.drug ,Transcription Factors - Abstract
Doxorubicin (DOX) is an antineoplastic agent that is widely employed in carcinomas, but it can cause cardiotoxicity in clinic. TRIM25 has E3 ubiquitin ligase activities and can ubiquitinate its target proteins. The role of TRIM25 in DOX-induced cardiotoxicity remains unknown. In this study, our results showed that DOX induced pyroptosis of H9c2 cells by TUNEL staining and Western blot assay. Interestingly, TRIM25 was downregulated in DOX-treated H9c2 cells in a time- and dose-dependent manner. TRIM25 attenuated DOX-induced pyroptosis of H9c2 cells. Furthermore, in vitro ubiquitination assay proved that TRIM25 decreased the stability of NLRP1 via promoting the ubiquitination of NLRP1. The rescue experiments confirmed that TRIM25 inhibited DOX-induced H9c2 cells pyroptosis by regulating NLRP1 stability. Animal experiments demonstrated that overexpression of TRIM25 attenuated DOX-induced cardiomyocyte pyroptosis in rats. In summary, TRIM25 exerts its cardioprotective effects by promoting the ubiquitination of NLRP1 in DOX-induced cardiomyocyte pyroptosis, which provides a novel therapeutic strategy for DOX-induced cardiotoxicity.
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- 2021
20. The effect of CYP2C19 genotype-guided antiplatelet therapy on outcomes of selective percutaneous coronary intervention patients: an observational study
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Jianhui Tian, Mengwan Li, Yan Shi, Zhexun Lian, Haichu Yu, Xiaxia Wang, Kai Tan, and Yiping Ge
- Subjects
0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Percutaneous coronary intervention ,General Medicine ,CYP2C19 ,030105 genetics & heredity ,medicine.disease ,Clopidogrel ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Conventional PCI ,medicine ,Molecular Medicine ,Observational study ,cardiovascular diseases ,Myocardial infarction ,business ,Ticagrelor ,Mace ,medicine.drug - Abstract
Aim: To observe if personalized antiplatelet therapy according to the CYP2C19 phenotype can improve the outcomes of patients receiving selective percutaneous coronary intervention (PCI). Methods: In this observational study, 677 Chinese patients undergoing selective PCI were divided into gene group (n = 369) and conventional group (n = 308), and given antiplatelet therapy according to the CYP2C19 genotype or clinical features, respectively. Incidence of MACE (death, non-fatal myocardial infarction, and unplanned repeat revascularization) and bleeding was compared between the two groups after 18 months. Results: Diabetes, heart dysfunction and SYNTAX score (>15), but not routinely CYP2C19 genotype test-guided antiplatelet therapy, were associated with MACE. The incidence of bleeding showed no difference. Conclusion: CYP2C19 phenotype-guided antiplatelet therapy may have no influence on the outcomes of selective PCI patients. Clinical features-guided antiplatelet therapy may be reasonable.
- Published
- 2019
21. Association between interleukin-8 gene -251 A/T polymorphism and the risk of coronary artery disease: A meta-analysis
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Jun Wu, Zuo-Yuan Chen, Quan-Fang Zhang, Wenzhong Zhang, Wei Wang, Wugang Wang, Yan Cui, and Zhexun Lian
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,China ,Heterozygote ,Genotype ,Coronary Artery Disease ,polymorphism ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Internal medicine ,medicine ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,030212 general & internal medicine ,Allele ,Acute Coronary Syndrome ,Genotyping ,Alleles ,Aged ,business.industry ,Homozygote ,Interleukin-8 ,Case-control study ,Heterozygote advantage ,General Medicine ,Odds ratio ,Middle Aged ,meta-analysis ,030220 oncology & carcinogenesis ,Meta-analysis ,Case-Control Studies ,Female ,Gene polymorphism ,business ,Polymorphism, Restriction Fragment Length ,Systematic Review and Meta-Analysis ,Research Article - Abstract
Background: The association between interleukin-8 (IL-8) gene polymorphism −251 A>T and susceptibility to coronary artery disease (CAD) has been investigated previously; however, results remain controversial. Thus, a meta-analysis was conducted to reassess the effects of this polymorphism on CAD risks. Methods: The PubMed, Cochrane Library, China National Knowledge Infrastructure, and Wanfang databases were searched for relevant studies published up to December, 2018. The pooled odds ratios (OR) were calculated using STATA 13.0 software for allelic (A vs T) as well as homozygote (AA vs TT), heterozygote (AT vs TT), recessive (AA vs AT + TT), and dominant (AA + AT vs TT) genotype models, respectively. Results: Ten case-control studies (3744 cases and 3660 controls) were included. Overall, a significant association of IL-8 gene −251 A > T polymorphism with an increased risk of CAD was only observed in the dominant genotype model (OR = 1.48), but not others. In the subgroup analysis, significantly increased risks were also found for Chinese (OR = 1.64), polymerase chain reaction-restriction fragment length polymorphism genotyping (OR = 1.61), acute coronary syndrome (ACS) type (OR = 1.92 for 3 datasets; OR = 1.88 for 4 datasets), high quality (OR = 1.64), and age/gender matching status (OR = 1.55) under the dominant model. Furthermore, significantly increased risks were also found for ACS type under allelic (OR = 1.32 for 3 datasets; OR = 127 for 4 datasets), homozygote (OR = 1.64 for 3 datasets; OR = 1.50 for 4 datasets), heterozygote (OR = 1.32 for 3 datasets; OR = 1.30 for 4 datasets), and recessive (OR = 1.40 for 3 datasets; OR = 1.28 for 4 datasets) models. Conclusion: This meta-analysis suggests that Chinese patients carrying −251A allele of IL-8 may have an increased risk for the development of CAD, especially ACS.
- Published
- 2019
22. Understanding the role of non-coding RNA (ncRNA) in stent restenosis
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Muhammad Tariq, Murugavel Ponnusamy, Jiawei Tian, Ningning Tang, Tao Yu, Zhexun Lian, Shaoyan Liu, Shaoyan Jiang, Hui Xin, and Yanyan Yang
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0301 basic medicine ,Neointima ,RNA, Untranslated ,Vascular smooth muscle ,medicine.medical_treatment ,Coronary Disease ,Inflammation ,Coronary Artery Disease ,Bioinformatics ,Muscle, Smooth, Vascular ,Coronary Restenosis ,Coronary artery disease ,03 medical and health sciences ,Percutaneous Coronary Intervention ,Restenosis ,Animals ,Humans ,Medicine ,cardiovascular diseases ,RNA, Small Interfering ,business.industry ,Endothelial Cells ,Percutaneous coronary intervention ,Stent ,Drug-Eluting Stents ,medicine.disease ,Thrombosis ,MicroRNAs ,030104 developmental biology ,Stents ,Endothelium, Vascular ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Proto-Oncogene Proteins c-akt - Abstract
Coronary heart disease (CHD) is one of the leading disorders with the highest mortality rate. Percutaneous angioplasty and stent implantation are the currently available standard methods for the treatment of obstructive coronary artery disease. However, the stent being an exogenous substance causes several complications by promoting the proliferation of vascular smooth muscle cells, immune responses and neointima formation after implantation, leading to post-stent restenosis (ISR) and late thrombosis. The prevention of these adverse vascular events is important to achieve long-term proper functioning of the heart after stent implantation. Non-coding ribonucleic acids (ncRNAs) are RNA molecules not translated into proteins, theyhave a great potential in regulating endothelial cell and vascular smooth muscle function as well as inflammatory reactions. In this review, we outline the regulatory functions of different classes of ncRNA in cardiovascular disease and propose ncRNAs as new targets for stent restonosis treatment.
- Published
- 2018
23. Prediction model of in-hospital mortality in intensive care unit patients with heart failure: machine learning-based, retrospective analysis of the MIMIC-III database
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Hui Xin, Mingqiang Fu, Fuhai Li, Zhexun Lian, Jidong Zhang, and Jingmin Zhou
- Subjects
intensive & critical care ,0301 basic medicine ,Critical Care ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,Logistic regression ,computer.software_genre ,Machine Learning ,03 medical and health sciences ,0302 clinical medicine ,Lasso (statistics) ,Intensive care ,Linear regression ,Humans ,Medicine ,Hospital Mortality ,adult intensive & critical care ,Retrospective Studies ,Heart Failure ,Framingham Risk Score ,Database ,business.industry ,clinical audit ,Regression analysis ,Retrospective cohort study ,General Medicine ,Nomogram ,Intensive Care Units ,030104 developmental biology ,business ,computer - Abstract
ObjectiveThe predictors of in-hospital mortality for intensive care units (ICUs)-admitted heart failure (HF) patients remain poorly characterised. We aimed to develop and validate a prediction model for all-cause in-hospital mortality among ICU-admitted HF patients.DesignA retrospective cohort study.Setting and participantsData were extracted from the Medical Information Mart for Intensive Care (MIMIC-III) database. Data on 1177 heart failure patients were analysed.MethodsPatients meeting the inclusion criteria were identified from the MIMIC-III database and randomly divided into derivation (n=825, 70%) and a validation (n=352, 30%) group. Independent risk factors for in-hospital mortality were screened using the extreme gradient boosting (XGBoost) and the least absolute shrinkage and selection operator (LASSO) regression models in the derivation sample. Multivariate logistic regression analysis was used to build prediction models in derivation group, and then validated in validation cohort. Discrimination, calibration and clinical usefulness of the predicting model were assessed using the C-index, calibration plot and decision curve analysis. After pairwise comparison, the best performing model was chosen to build a nomogram according to the regression coefficients.ResultsAmong the 1177 admissions, in-hospital mortality was 13.52%. In both groups, the XGBoost, LASSO regression and Get With the Guidelines-Heart Failure (GWTG-HF) risk score models showed acceptable discrimination. The XGBoost and LASSO regression models also showed good calibration. In pairwise comparison, the prediction effectiveness was higher with the XGBoost and LASSO regression models than with the GWTG-HF risk score model (pConclusionsOur nomogram enabled good prediction of in-hospital mortality in ICU-admitted HF patients, which may help clinical decision-making for such patients.
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- 2021
24. Assessment of aflatoxin B1 myocardial toxicity in rats: mitochondrial damage and cellular apoptosis in cardiomyocytes induced by aflatoxin B1
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Zhexun Lian, Jian Li, Hao Fang, Lusha Qian, Hongjing Zhang, Haichu Yu, and Junhua Ge
- Subjects
0301 basic medicine ,caspase-3 ,Aflatoxin ,Aflatoxin B1 ,Apoptosis ,Caspase 3 ,Mitochondrion ,Pharmacology ,medicine.disease_cause ,Biochemistry ,Mitochondria, Heart ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Myocytes, Cardiac ,Bcl-2 ,Inner mitochondrial membrane ,Carcinogen ,cell apoptosis ,biology ,Toxin ,Biochemistry (medical) ,myocardial toxicity ,Heart ,Research Reports ,Cell Biology ,General Medicine ,biology.organism_classification ,Molecular biology ,Aspergillus parasiticus ,Rats ,mitochondria ,030104 developmental biology ,Proto-Oncogene Proteins c-bcl-2 ,030220 oncology & carcinogenesis ,Microscopy, Electron, Scanning ,Female - Abstract
Objective The number of deaths from heart disease is increasing worldwide. Aflatoxin B1 (AFB1), a toxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus, is frequently detected in improperly processed/stored human food products. While AFB1 hepatotoxicity and carcinogenic properties have been well addressed, its myocardial toxicity is poorly documented. This study aimed to investigate myocardial toxic activity of AFB1. Methods Ten rats were fed with AFB1 at a dose that did not result in acute toxic reactions for 30 days and 10 vehicle-fed rats served as controls. Transmission electron microscopy was used to assess mitochondrial damage in cardiomyocytes. The terminal deoxynucleotidyl transferase-mediated UTP nick-end labelling assay was performed to detect apoptosis of cardiomyocytes. Western blotting was performed to measure apoptotic proteins (i.e., active caspase-3, Bax, and Bcl-2) in heart tissue. Results AFB1 treatment resulted in mitochondrial membrane disruption and disorganization of cristae, which are indicators of mitochondrial damage. Myocardial cell apoptosis was significantly higher after AFB1 treatment (22.07% ± 3.29%) compared with controls (6.27% ± 2.78%, P Conclusion Various adverse effects are exerted by AFB1 on the heart, indicating AFB1 myocardial toxicity.
- Published
- 2017
25. The effect of
- Author
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Kai, Tan, Zhexun, Lian, Yan, Shi, Xiaxia, Wang, Haichu, Yu, Mengwan, Li, Jianhui, Tian, and Yiping, Ge
- Subjects
Cytochrome P-450 CYP2C19 ,China ,Percutaneous Coronary Intervention ,Genotype ,Heart Diseases ,Pharmacogenomic Variants ,Incidence ,Humans ,Postoperative Hemorrhage ,Precision Medicine ,Prognosis ,Platelet Aggregation Inhibitors - Published
- 2019
26. ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway
- Author
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Jun-Hua Fu, Ru-Yong Yao, Fang Wang, Zhexun Lian, Zuo-Yuan Chen, and Hui Xin
- Subjects
0301 basic medicine ,Cancer Research ,Ischemia ,030204 cardiovascular system & hematology ,Pharmacology ,Wortmannin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,myocardial reperfusion injury ,medicine ,Protein kinase B ,Cardioprotection ,adenosine-5′-triphosphate-dependent potassium channels ,Kinase ,business.industry ,adenosine-5′-triphosphate ,General Medicine ,Articles ,medicine.disease ,Molecular biology ,Potassium channel ,030104 developmental biology ,chemistry ,Apoptosis ,reperfusion injury salvage kinase ,business ,Reperfusion injury - Abstract
The aim of the present study was to examine the post-infarct acute effect of adenosine-5'-triphosphate (ATP) on myocardial infarction (MI) size as well as its precise molecular mechanism. Sixty New Zealand white male rabbits were exposed to 40 min of ischemia followed by 180 min of reperfusion. The rabbits were intravenously administered 3 mg/kg of ATP (ATP group) or saline (control group) immediately after reperfusion and maintained throughout the first 30 min. The wortmannin+ATP, PD-98059+ATP, and 5-hydroxydecanoic acid (5-HD) sodium salt+ATP groups were separately injected with wortmannin (0.6 mg/kg), PD-98059 (0.3 mg/kg), and 5-HD (5 mg/kg) 5 min prior to ATP administration. MI size was calculated as the percentage of the risk area in the left ventricle. Myocardial apoptosis was determined using a TUNEL assay. Western blot analysis was performed to examine the levels of protein kinase B (Akt)/p-Akt and extracellular signal-regulated kinase (ERK)/p-ERK in the ischemic myocardium, 180 min after reperfusion. The infarct size was significantly smaller in the ATP group than in the control group (p
- Published
- 2016
27. Association between interleukin-8 gene -251 A/T polymorphism and the risk of coronary artery disease: A meta-analysis.
- Author
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Quanfang Zhang, Zhexun Lian, Wenzhong Zhang, Yan Cui, Wugang Wang, Jun Wu, Zuoyuan Chen, Wei Wang, Zhang, Quanfang, Lian, Zhexun, Zhang, Wenzhong, Cui, Yan, Wang, Wugang, Wu, Jun, Chen, Zuoyuan, and Wang, Wei
- Published
- 2019
- Full Text
- View/download PDF
28. Elevated Lipoprotein-Associated Phospholipase A2 Is Associated with Progression of Nonculprit Lesions after Percutaneous Coronary Intervention
- Author
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Wei-Qiang Kang, Rui Zhang, Hui-Ping Gong, Shanglang Cai, Song Liu, Hui Xin, Quan-Fang Zhang, Zhexun Lian, Zuo-Yuan Chen, Xian-feng Ning, and Zhi-Ming Ge
- Subjects
Male ,Acute coronary syndrome ,medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,Coronary Angiography ,General Biochemistry, Genetics and Molecular Biology ,Body Mass Index ,Pathogenesis ,Percutaneous Coronary Intervention ,Internal medicine ,medicine ,Humans ,Acute Coronary Syndrome ,Phospholipids ,Aged ,Anthropometry ,business.industry ,Hydrolysis ,Lipoprotein-associated phospholipase A2 ,Percutaneous coronary intervention ,General Medicine ,Middle Aged ,medicine.disease ,Oxygen ,Stenosis ,Cholesterol ,1-Alkyl-2-acetylglycerophosphocholine Esterase ,Multivariate Analysis ,Conventional PCI ,Disease Progression ,Cardiology ,Female ,business ,Body mass index ,Biomarkers ,Follow-Up Studies ,Blood sampling - Abstract
Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an enzyme that hydrolyzes oxidized phospholipids to generate bioactive proatherogenic products. Nonculprit lesions have been assumed to contribute to the pathogenesis of recurrent acute coronary syndrome (ACS). The role of LP-PLA2 in the progression of nonculprit coronary lesions after successful percutaneous coronary intervention (PCI) remains unclear. Our study included 123 patients with ACS who underwent initial PCI and a long-term follow-up (mean interval, one year) with coronary angiography. Among them, 19 patients were diagnosed as the progression of nonculprit lesions, based on the presence of at least one of the following factors: (1) ≥ 10% reduction in the diameter of a preexisting ≥ 50% stenosis; (2) ≥ 30% reduction in the diameter of a < 50% stenosis; and (3) early-onset stenosis with ≥ 30% reduction in the diameter of a segment that was normal on the primary angiogram. Blood sampling was drawn from all patients at 12-14 hours after PCI. The ACS patients with progression had higher total cholesterol (4.47 ± 1.02 mmol/L vs. 3.59 ± 0.57 mmol/L, P < 0.05), higher levels of Lp-PLA2 activity (14.39 ± 6.13 nmol/min/ml vs. 8.86 ± 3.14 nmol/min/ml, P < 0.001) and a higher proportion of multi-vessel disease than those without progression. Multivariate logistic regression analysis showed that Lp-PLA2 activity (β = 0.024, P = 0.005) was an independent predictor for rapid progression of nonculprit coronary lesions. In conclusion, elevated Lp-PLA2 activity is associated with rapid progression of nonculprit coronary lesions in ACS patients who underwent PCI.
- Published
- 2013
29. miRNA-9 inhibits apoptosis and promotes proliferation in angiotensin II-induced human umbilical vein endothelial cells by targeting MDGA2.
- Author
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Kai Tan, Yiping Ge, Jianhui Tian, Shaohua Li, Zhexun Lian, Tan, Kai, Ge, Yiping, Tian, Jianhui, Li, Shaohua, and Lian, Zhexun
- Abstract
Hypertension is a universal risk factor for a variety of cardiovascular diseases. Investigation of the mechanism for hypertension will benefit around 40% of the world's adult population. MicroRNA is crucial for the initiation and progression of cardiovascular diseases. In this study, angiotensin II-treated human umbilical vein endothelial cells were used as a model to imitate the pathological changes in endothelial cells under hypertensive conditions. We demonstrated that microRNA-9 (miR-9) suppressed angiotensin II-induced apoptosis and enhanced proliferation in human umbilical vein endothelial cells. Direct interaction between miR-9 and mitochondria associated membrance domain containing glycosylphosphatidylinositol anchor 2 (MDGA2) was determined. Moreover, miR-9 suppressed MDGA2 levels by binding to the 3' UTR site of the MDGA2 gene. This negative regulation of MDGA2 by miR-9 significantly increased proliferation and decreased apoptosis. Re-introduction of MDGA2 in the miR-9 overexpressed human umbilical vein endothelial cells and normalized proliferation, apoptosis, and the cell cycle. In summary, the present study demonstrated miR-9 inhibited expression of MDGA2 leading to the inhibition of apoptosis and promotion of proliferation in angiotensin II-treated human umbilical vein endothelial cells. [ABSTRACT FROM AUTHOR]
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- 2019
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30. Detection of P16 and APC gene methylation in peripheral blood with liver cancer patients
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Jin-Mei Piao, Fu-Guo Yang, Zhexun Lian, Hee Nam Kim, Cheng-Cheng Li, Meng Liu, Yang Song, Min-Ho Shin, and Lianhua Cui
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Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,DNA methylation ,medicine ,Cancer research ,Liver cancer ,medicine.disease ,business ,Peripheral blood - Published
- 2014
31. Transcatheter Treatment of Large Aortopulmonary Window: A Case Report
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Quansheng Xing, Zuo-Yuan Chen, Daxin Zhou, Qin Wu, Pin Sun, Zhexun Lian, Xiaxia Wang, Hui Xin, and Jidong Zhang
- Subjects
Cardiac Catheterization ,medicine.medical_specialty ,Aortopulmonary septal defect ,Septal Occluder Device ,business.industry ,Hypertension, Pulmonary ,medicine.medical_treatment ,medicine.disease ,Pulmonary hypertension ,Aortopulmonary Septal Defect ,Aortopulmonary window ,Surgery ,Shunt (medical) ,Young Adult ,Internal medicine ,MUSCULAR VENTRICULAR SEPTAL DEFECT ,medicine ,Cardiology ,Humans ,Female ,Cardiology and Cardiovascular Medicine ,business ,Cardiac catheterization - Abstract
Introduction: Aortopulmonary window (APW), a large aortopulmonary septal defect (APSD), is a serious and rare defect within congenital heart diseases. Case report: In this study, we reported an APW case with severe pulmonary arterial hypertension. This patient was successfully treated by transcatheter closure with a muscular ventricular septal defect (VSD) occluder. Conclusion: We had a successful experience with transcatheter closure of a large APW using a muscular VSD occluder. There was no residual shunt or complications during the 6-month follow-up.
- Published
- 2015
32. Assessment of aflatoxin B1 myocardial toxicity in rats: mitochondrial damage and cellular apoptosis in cardiomyocytes induced by aflatoxin B1.
- Author
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Junhua Ge, Haichu Yu, Jian Li, Zhexun Lian, Hongjing Zhang, Hao Fang, and Lusha Qian
- Published
- 2017
- Full Text
- View/download PDF
33. Atp-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through risk pathway
- Author
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F. Wang, Zuo-Yuan Chen, Zhexun Lian, Ru-Yong Yao, Jidong Zhang, Shanglang Cai, and Hui Xin
- Subjects
Cardioprotection ,TUNEL assay ,Kinase ,business.industry ,Ischemia ,Pharmacology ,medicine.disease ,Wortmannin ,chemistry.chemical_compound ,chemistry ,Apoptosis ,Anesthesia ,medicine ,Cardiology and Cardiovascular Medicine ,business ,Protein kinase B ,Reperfusion injury - Abstract
Objective: Adenosine-5'-triphosphate (ATP) has been reported to protect the heart from myocardial ischemia/reperfusion injury when administered at the onset of reperfusion and is as effective as mechanical postconditioning. However, whether the protective effect of postconditioning with ATP is mediated through the same signaling pathways as those of ischemic postconditioning is still unclear. We examined the postinfarct acute effect of ATP on myocardial infarct size and its precise molecular mechanism. Methods: Forty-eight New Zealand white male rabbits were exposed to 40 min of ischemia followed by 180 min of reperfusion. Rabbits were intravenously injected 3mg/kg of ATP (ATP group) or saline (control group) immediately after reperfusion within 30min. The wortmannin+ATP, PD-98059+ATP, and 5-hydroxydecanoic acid sodium salt (5-HD)+ATP groups were respectively injected with wortmannin [a phosphatidyl-inositol 3-kinase (PI3-kinase) inhibitor, 0.6 mg/kg], PD-98059 [an extracellular signal regulated protein kinase (ERK) inhibitor, 0.3 mg/kg], and 5-HD [a mitochondrial ATP-dependent K+ (KATP) channel blocker, 5 mg/kg] 5 min before ATP administration. Myocardial infarct size was calculated as a percentage of the risk area of the left ventricle. Myocardial apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) methods. Western blot analysis was performed to examine the signals such as protein kinase B (Akt) and extracellular signal-regulated protein kinase (ERK) in the ischemic myocardium after 180min of reperfusion. Results: The infarct size was significantly smaller in the ATP group (12.8±1.9%) than in the control group (29.1±2.9%). The infarct size-reducing effect of ATP was completely blocked by wortmannin (26.5±2.7%), PD-98059 (27.9±3.2%), and 5-HD (26.1±4.0%). Compared with control group, ATP group significantly reduced cardiomyocytes apoptosis (apoptotic index: 27.0±5.8% vs 10.3±6.0%, P
- Published
- 2013
34. Transcatheter Treatment of Large Aortopulmonary Window: A Case Report.
- Author
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Zuoyuan Chen, Jidong Zhang, Xiaxia Wang, Quansheng Xing, Hui Xin, \, Zhexun Lian, Pin Sun, Qin Wu, and Daxin Zhou
- Published
- 2015
- Full Text
- View/download PDF
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