126 results on '"Zhihe Qing"'
Search Results
2. Platinum Nanoparticles Loaded Graphitic Carbon Nitride Nanosheets with Enhanced Peroxidase-like Activity for H2O2 and Oxidase-Based Sensing
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Gege Yang, Ying Chen, Rui Shi, Rongrong Chen, Shanshan Gao, Xin Zhang, Yuan Rao, Ying Lu, Yuancheng Peng, Zhihe Qing, and Chunxia Song
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PtNP@g–C3N4 nanosheets ,peroxidase-mimic ,H2O2 ,oxidase-based sensing ,Organic chemistry ,QD241-441 - Abstract
Platinum nanoparticles (PtNPs) are classical peroxidase-like nanozyme; self-agglomeration of nanoparticles leads to the undesirable reduction in stability and catalytic activity. Herein, a hybrid peroxidase-like nanocatalyst consisting of PtNPs in situ growing on g–C3N4 nanosheets with enhanced peroxidase-mimic catalytic activity (PtNP@g–C3N4 nanosheets) was prepared for H2O2 and oxidase-based colorimetric assay. g–C3N4 nanosheets can be used as carriers to solve the problem of poor stability of PtNPs. We observed that the catalytic ability could be maintained for more than 90 days. PtNP@g–C3N4 nanosheets could quickly catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), and the absorbance of blue color oxidized TMB (oxTMB) showed a robust linear relationship with the concentration of H2O2 (the detection limit (LOD): 3.33 μM). By utilizing H2O2 as a mediator, this strategy can be applied to oxidase-based biomolecules (glucose, organophosphorus, and so on, that generate or consume hydrogen peroxide) sensing. As a proof of concept, a sensitive assay of cholesterol that combined PtNP@g–C3N4 nanosheets with cholesterol oxidase (ChOx) cascade catalytic reaction was constructed with an LOD of 9.35 μM in a widespread range from 10 to 800 μM (R2 = 0.9981). In addition, we also verified its ability to detect cholesterol in fetal bovine serum. These results showed application prospect of PtNP@g–C3N4 nanosheets-based colorimetry in sensing and clinical medical detection.
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- 2023
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3. Editorial: Advances in Nucleic Acid-Based Biosensors and Imaging
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Jane Ru Choi, Mithran Somasundrum, Muhammad J.A. Shiddiky, Werasak Surareungchai, Yufang Hu, and Zhihe Qing
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nucleic acid ,DNA/RNA ,sensing ,diagnostics ,imaging ,detection ,Chemistry ,QD1-999 - Published
- 2022
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4. MIL/Aptamer as a Nanosensor Capable of Resisting Nonspecific Displacement for ATP Imaging in Living Cells
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Jun Li, Yuedong Zhang, Zhen Zou, Zhihe Qing, Sheng Yang, Jianxiao Yang, Lihua Zhang, Feng Feng, and Ronghua Yang
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Chemistry ,QD1-999 - Published
- 2019
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5. Natural Peptide Probe Screened for High-Performance Fluorescent Sensing of Copper Ion: Especially Sensitivity, Rapidity, and Environment-Friendliness
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Xiaoxuan Li, Zhihe Qing, Younan Li, Zhen Zou, Sheng Yang, and Ronghua Yang
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Chemistry ,QD1-999 - Published
- 2019
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6. DNA-Templated Fluorescent Nanoclusters for Metal Ions Detection
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Chunxia Song, Jingyuan Xu, Ying Chen, Liangliang Zhang, Ying Lu, and Zhihe Qing
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dna-template ,fluorescence ,nanoclusters ,metal ions ,detection ,Organic chemistry ,QD241-441 - Abstract
DNA-templated fluorescent nanoclusters (NCs) have attracted increasing research interest on account of their prominent features, such as DNA sequence-dependent fluorescence, easy functionalization, wide availability, water solubility, and excellent biocompatibility. Coupling DNA templates with complementary DNA, aptamers, G-quadruplex, and so on has generated a large number of sensors. Additionally, the preparation and applications of DNA-templated fluorescent NCs in these sensing have been widely studied. This review firstly focuses on the properties of DNA-templated fluorescent NCs, and the synthesis of DNA-templated fluorescent NCs with different metals is then discussed. In the third part, we mainly introduce the applications of DNA-templated fluorescent NCs for sensing metal ions. At last, we further discuss the future perspectives of DNA-templated fluorescent NCs in the synthesis and sensing metal ions in the environmental and biological fields.
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- 2019
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7. A Reaction-Based Ratiometric Bioluminescent Platform for Point-of-Care and Quantitative Detection Using a Smartphone
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Junbin Li, Na Wang, Mengyi Xiong, Min Dai, Cheng Xie, Qianqian Wang, Ke Quan, Yibo Zhou, and Zhihe Qing
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Analytical Chemistry - Published
- 2023
8. Brain-targeted Near-Infrared Nanobeacon for In Situ Monitoring H2S Fluctuation during Epileptic Seizures
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Junbin Li, Ying Zhou, Lifei Song, Sheng Yang, Qianqian Wang, Yibo Zhou, Xiao-Bing Zhang, Zhihe Qing, and Ronghua Yang
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Analytical Chemistry - Published
- 2022
9. Rigidity-Dependent Emission: Inspired Selection of an ATP-Specific Polyvalent Hydrogen Binding-Lighted Fluorophore for Intracellular Amplified Imaging
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Yibo Zhou, Xiaofei Ma, Shan Hu, Sheng Yang, Jingru Guo, Junbin Li, Yue-Fei Zhang, Juewen Liu, Zhihe Qing, and Ronghua Yang
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Analytical Chemistry - Published
- 2023
10. Target-triggered hairpin-free chain-branching growth of DNA dendrimers for contrast-enhanced imaging in living cells by avoiding signal dispersion
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Jingyuan Xu, Minzhi Ouyang, Yang Ronghua, Yanli Lei, Zhen Zou, Junbin Li, Zhihe Qing, and Jiaqi Deng
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In situ ,chemistry.chemical_compound ,chemistry ,Dendrimer ,Dispersion (optics) ,Biophysics ,General Chemistry ,Transfection ,Branching (polymer chemistry) ,Fluorescence ,Signal ,DNA - Abstract
The development of amplification strategies is one of the central challenges for detection of low-abundance targets. One-to-many (1:M) amplification strategies in which one target lights many signal probes, has improved the detection sensitivity in bulk solution, but with discounted contrast in cell imaging, because the lighted probes are dissociative and dispersible. In this work, a one-to-large (1:L) signaling mechanism, in which the lighted probes were orderly connected to each other, was conceptually proposed to enhance the contrast in cell imaging by avoiding signal dispersion in amplification. Accordingly, target-triggered hairpin-free chain-branching assembly (HFCBA) holds great potential to implement the 1:L mechanism, but using it in cell imaging has yet to be demonstrated. As a proof of concept, a group of probes were first programmed to implement miRNA-21-triggered HFCBA. After transfection of probes, gradually-growing signal flares in cells were monitored along with the growth of DNA dendrimers; and the in situ fluorescence accumulation in HFCBA resulted in highly-enhanced contrast to the surrounding by avoiding signal dispersion in amplification. The contrast-enhanced imaging with signal amplification is significant for biological analysis and molecular medicine. We expect the 1:L mechanism will provide a new thought for high-performance imaging of biomarkers in cells.
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- 2022
11. Zn 2+ ‐Coordination‐Driven RNA Assembly with Retained Integrity and Biological Functions
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Zhen Zou, Libei He, Xiangxi Deng, Huangxiang Wang, Ziyun Huang, Qian Xue, Zhihe Qing, Yanli Lei, Ronghua Yang, and Juewen Liu
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General Medicine - Published
- 2021
12. Zn 2+ ‐Coordination‐Driven RNA Assembly with Retained Integrity and Biological Functions
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Libei He, Ziyun Huang, Qian Xue, Zhihe Qing, Yanli Lei, Xiangxi Deng, Ronghua Yang, Zhen Zou, Juewen Liu, and Huangxiang Wang
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chemistry.chemical_classification ,Messenger RNA ,Biomolecule ,RNA ,General Chemistry ,Gene delivery ,Catalysis ,chemistry.chemical_compound ,chemistry ,microRNA ,Biophysics ,Nanomedicine ,Nanobiotechnology ,DNA - Abstract
Metal-coordination-directed biomolecule crosslinking in nature has been used for synthesizing various biopolymers, including DNA, peptides, proteins, and polysaccharides. However, the RNA biopolymer has been avoided so far, as due to the poor stability of the RNA molecules, the formation of a biopolymer may alter the biological function of the molecules. Herein, for the first time, we report Zn2+ -driven RNA self-assembly forming spherical nanoparticles while retaining the integrity and biological function of RNA. Various functional RNAs of different compositions, shapes, and lengths from 20 to nearly 1000 nucleotides were used, highlighting the versatility of this approach. The assembled nanospheres possess a superior RNA-loading efficiency, pharmacokinetics, and bioavailability. In-vitro and in-vivo evaluation demonstrated mRNA delivery for expressing GFP proteins, and microRNA delivery to triple-negative breast cancer. This coordination-directed self-assembly behavior amplifies the horizons of RNA coordination chemistry and the application scope of RNA-based therapeutics.
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- 2021
13. A Glucose‐Powered Activatable Nanozyme Breaking pH and H 2 O 2 Limitations for Treating Diabetic Infections
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Qiaoshu Chen, Shuohui Xing, Ke Quan, Lifang Chen, Zhihe Qing, Juewen Liu, Yanli Lei, Yang Ronghua, and Zhen Zou
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biology ,Aptamer ,General Medicine ,General Chemistry ,Antibacterial effect ,biology.organism_classification ,Catalysis ,In vitro ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Hyaluronidase ,Hyaluronic acid ,medicine ,biology.protein ,Glucose oxidase ,Bacteria ,medicine.drug - Abstract
The peroxidase-like activity of nanozymes is promising for chemodynamic therapy by catalyzing H2 O2 into . OH. However, for most nanozymes, this activity is optimal just in acidic solutions, while the pH of most physiological systems is beyond 7.0 (even >8.0 in chronic wounds) with inadequate H2 O2 . We herein communicate an activatable nanozyme with targeting capability to simultaneously break the local pH and H2 O2 limitations under physiological conditions. As a proof of concept, aptamer-functionalized nanozymes, glucose oxidase, and hyaluronic acid constitute an activatable nanocapsule "APGH", which can be activated by bacteria-secreted hyaluronidase in infected wounds. Nanozymes bind onto bacteria through aptamer recognition, and glucose oxidation tunes the local pH down and supplements H2 O2 for the in-situ generation of . OH on bacteria surfaces. The activity switching and enhanced antibacterial effect of the nanocapsule were verified in vitro and in diabetic wounds. This strategy for directly regulating local microenvironment is generally accessible for nanozymes, and significant for facilitating biological applications of nanozymes.
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- 2021
14. An Activatable Nanoenzyme Reactor for Coenhanced Chemodynamic and Starving Therapy Against Tumor Hypoxia and Antioxidant Defense System
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Juewen Liu, Zhihe Qing, JunBin Li, Yanli Lei, Lifang Chen, Ailing Bai, Shuohui Xing, Ronghua Yang, and Zhen Zou
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Antioxidant ,Tumor hypoxia ,business.industry ,medicine.medical_treatment ,medicine ,Cancer research ,Cancer therapy ,General Chemistry ,business ,Therapeutic strategy - Abstract
It is critical to improve the efficiency of cancer therapy with minimized side effects. Chemodynamic therapy (CDT) is a tumor therapeutic strategy designed to generate abundant reactive oxygen spec...
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- 2021
15. Bidirectional modulation of microRNA with a clamp-like triplex switch for enhanced and programmed gene therapy
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Yu Zeng, Zhihe Qing, Lihua Zhang, Zhen Zou, Ronghua Yang, Yanli Lei, Feng Feng, and Haiyan Sun
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Chemistry ,Genetic enhancement ,Metals and Alloys ,Genetic Therapy ,General Chemistry ,Catalysis ,In vitro ,nervous system diseases ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Cell biology ,MicroRNAs ,Clamp ,Downregulation and upregulation ,Modulation ,Neoplasms ,microRNA ,Materials Chemistry ,Ceramics and Composites ,Humans - Abstract
A clamp-like triplex switch (CTS) able to simultaneously downregulate an overexpressed onco-miRNA and replenish the lost tumor-suppressive miRNA in a controllable manner was developed for enhanced gene therapy. Compared to the "unidirectional" regulation approach, the CTS displayed improved anti-tumor efficacy in vitro and was harmless to healthy cells.
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- 2021
16. Synthesis and Characterization of DNA-Mediated Fluorescent Copper Nanoclusters: Recommending A Research-oriented Comprehensive Chemical Experiment
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Junbin Li, Chenglin Li, Jiaqi Luo, Hui Chen, and Zhihe Qing
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General Medicine - Published
- 2023
17. A Polymeric Nanobeacon for Monitoring the Fluctuation of Hydrogen Polysulfides during Fertilization and Embryonic Development
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Yibo Zhou, Zhengxuan Gu, Changhui Liu, Sheng Yang, Xiaofei Ma, Qiaoshu Chen, Yanli Lei, Ke Quan, Juewen Liu, Zhihe Qing, and Ronghua Yang
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Polymers ,Fertilization ,Animals ,Embryonic Development ,General Medicine ,General Chemistry ,Sulfides ,Zebrafish ,Catalysis ,Hydrogen - Abstract
Fertilization and early embryonic development as the beginning of a new life are key biological events. Hydrogen polysulfide (H
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- 2022
18. Pt–S Bond‐Mediated Nanoflares for High‐Fidelity Intracellular Applications by Avoiding Thiol Cleavage
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Zhihe Qing, Guoyan Luo, Shuohui Xing, Zhen Zou, Yanli Lei, Juewen Liu, and Ronghua Yang
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General Medicine - Published
- 2020
19. Visualization of Long Noncoding RNA MEG3 in Living Cells by a Triple-Helix-Powered 3D Catcher
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Ronghua Yang, Ziling Luo, Xuan Xu, Xiangxi Deng, Yufei Zhang, Zhen Zou, Qian Xue, Huanxiang Wang, Ziyun Huang, Zhihe Qing, and Hua Yang
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Biomaterials ,MEG3 ,Transactivation ,Fluorescence-lifetime imaging microscopy ,Chemistry ,Biochemistry (medical) ,Biomedical Engineering ,Regulator ,Tumor growth ,General Chemistry ,Long non-coding RNA ,Triple helix ,Cell biology - Abstract
Visual imaging of long noncoding RNA (lncRNA) MEG3, a newfound regulator of transactivation and tumor growth suppression, is conducive to unlock the secrets of MEG3 in some important biological processes. Here, for the first time, we designed a DNA tetrahedron-based three-dimensional (3D) catcher for imaging cytoplasmic lncRNA MEG3 in living cells. The 3D catcher is composed of a triple-helix-forming dsDNA with capacity to bind the 5'-end GA-rich domain of the lncRNA MEG3 and four hairpin-shaped antisense sequences toward contiguous domain on MEG3. Once ingested by the cell, the 3D catcher quickly captures lncRNA MEG3 via forming a DNA-RNA triple-helix structure and triggering the hybridization-based string disassembly of the catcher. Concomitantly, the quenched hairpin is opened and the fluorescent signal undergoes lighting on conversion. Ascribed to the triple-helix-induced "domino effect," the disassembly reaction time is greatly shorter than the reaction with the inability to form a triple helix. The 3D catcher allows detection of long-chain targets as long as 129 nucleotide (129 nt) with a detection limit of 0.36 nM and distinguishes endogenous lncRNA MEG3 fragments in living cells between hepatoma cells and normal hepatocytes, which provides a reliable strategy for monitoring endogenous long fragment nucleic acid biomarkers in early clinical lesion diagnoses.
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- 2020
20. Human Serum Albumin-Occupying-Based Fluorescence Turn-On Analysis of Antiepileptic Drug Tiagabine Hydrochloride
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Zhihe Qing, Lihua Zhang, Liao Xiaodou, Feng Feng, Hua Yang, Le Yang, Qi Yan, Ziyun Huang, Yufei Zhang, Zhen Zou, and Ronghua Yang
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Models, Molecular ,Drug ,Protein Conformation ,media_common.quotation_subject ,Serum albumin ,Serum Albumin, Human ,Biosensing Techniques ,Buffers ,Analytical Chemistry ,chemistry.chemical_compound ,Molecular recognition ,medicine ,Humans ,Tiagabine ,Imide ,media_common ,Chromatography ,Quenching (fluorescence) ,biology ,Human serum albumin ,Fluorescence ,Spectrometry, Fluorescence ,chemistry ,biology.protein ,Anticonvulsants ,Perylene ,medicine.drug - Abstract
Tiagabine hydrochloride (TGB) is a clinically frequently used drug for anticonvulsion and reducing epileptic frequency. Over administration of TGB could bring about adverse effects, such as speech disorder, depression, and even suicidal tendencies. Therefore, accessible and sensitive assay for analysis of TGB becomes an urgent need toward guiding clinical medication. Here, we present the first report on fluorescence turn-on detection of TGB in urine testing. In this protocol, a fluorescent dye, perylene tetracarboxylic acid imide derivative (PTAI), is found specifically occupying the Sudlow site II of human serum albumin (HSA) and displays a new phenomenon of binding-induced quenching (BIQ). In presence of TGB, competitive binding of the TGB to the site II of HSA will trigger release of PTAI, thus successfully lighting up the fluorescence of PTAI. This label-free assay enjoys a broader working range (1-350 μM) and lower detection limit (0.218 μM) than the traditional liquid chromatography method and is uninterfered by the miscellaneous in the artificial urine. The BIQ probe highlights the merits of HSA as a quencher and a molecular recognition unit, and it opens up a way for studying drug-HSA interaction mechanism and noninvasive pharmaceutical testing.
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- 2020
21. Human serum albumin as an intrinsic signal amplification amplifier for ultrasensitive assays of the prostate-specific antigen in human plasma
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Ronghua Yang, Zhihe Qing, Weihong Tan, Zhen Zou, Liping Qiu, Jing Zheng, Qi Yan, Haiyan Cai, Le Yang, and Peng Qi
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Models, Molecular ,Serum Albumin, Human ,Endogeny ,010402 general chemistry ,01 natural sciences ,Catalysis ,Antigen ,Materials Chemistry ,medicine ,Humans ,Fluorescent Dyes ,010405 organic chemistry ,Chemistry ,Amplifier ,Metals and Alloys ,General Chemistry ,Prostate-Specific Antigen ,Human serum albumin ,Molecular biology ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,body regions ,Butyrates ,Prostate-specific antigen ,Signal amplifier ,Spectrometry, Fluorescence ,Human plasma ,embryonic structures ,Ceramics and Composites ,Signal amplification ,medicine.drug - Abstract
As the most abundant protein in blood, human serum albumin (HSA) is usually regarded as an interferent in clinical molecular diagnosis. Herein, we report that HSA is an endogenous signal amplifier for the detection of the prostate-specific antigen (PSA) in human plasma. This is the first study to utilize intrinsic biological components as the signal amplifier in blood tests.
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- 2020
22. Al centre-powered graphitic nanozyme with high catalytic efficiency for pH-independent chemodynamic therapy of cancer
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Ke Yi, Jun Li, Zhihe Qing, Ronghua Yang, Yanli Lei, Zhen Zou, Haiyan Sun, and Yuedong Zhang
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Cell Survival ,Iron ,Mice, Nude ,Catalysis ,In vivo ,Cell Line, Tumor ,Neoplasms ,Nitriles ,Tumor Microenvironment ,Materials Chemistry ,medicine ,Animals ,Humans ,Catalytic efficiency ,Ph independent ,Tumor microenvironment ,Hydroxyl Radical ,Chemistry ,Metals and Alloys ,Tumor therapy ,Cancer ,Hydrogen Peroxide ,General Chemistry ,Hydrogen-Ion Concentration ,medicine.disease ,Combinatorial chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Methylene Blue ,Ceramics and Composites ,Graphite ,Aluminum - Abstract
An Al centre-powered graphitic nanozyme derived from a metal organic framework was first developed for a chemodynamic tumor treatment. By virtue of the rapid and efficient generation of ˙OH in the slightly acidic tumor microenvironment, this nanozyme afforded high anti-tumor efficacy both in living cells and in vivo.
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- 2020
23. Cytoplasmic Protein-Powered In Situ Fluorescence Amplification for Intracellular Assay of Low-Abundance Analyte
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Ronghua Yang, Yibo Zhou, Zhihe Qing, Sheng Yang, Yue Xiao, Zhen Zou, and Juewen Liu
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In situ ,Cytoplasm ,Analyte ,Fluorophore ,Chemical biology ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Analytical Chemistry ,Mice ,chemistry.chemical_compound ,Animals ,Humans ,Molecular Structure ,Optical Imaging ,010401 analytical chemistry ,RNA-Binding Proteins ,Nucleic acid amplification technique ,Flow Cytometry ,0104 chemical sciences ,Molecular Docking Simulation ,RAW 264.7 Cells ,chemistry ,Spectrophotometry ,MCF-7 Cells ,Biophysics ,Nucleic Acid Amplification Techniques ,Intracellular ,HeLa Cells - Abstract
Fluorescence amplification is critical for in situ and real-time detection of intracellular low abundance biological species. However, current intracellular amplification techniques mainly rely on synthetic nucleic acid-based nanodevices, manipulating them in living cells remains challenging. To solve this problem, herein, a new signal amplification concept named cytoplasmic protein-powered in situ fluorescence amplification (CPFA) is proposed. CPFA takes cytoplasmic protein as cell-self-power for signal amplification enabling it to operate in living cells. To establish a prototype of CPFA, an amplifiable sensor for hydroxyl radicals (•OH) was designed by entrapping the screened cytoplasmic protein-enhanced fluorophore (PBF1) inside mesoporous silica (MSN) nanocontainer with ssDNA/PTAD-based signal switch. When encountered with •OH in living cells, the ssDNA was cleaved to separate PTAD from MSN, liberating multiple copies of the loaded PBF1 to light up the fluorescence. Furthermore, these released PBF1 molecules can instantly bind with cytoplasmic proteins to amplify their fluorescence signals. Take advantage of this two-stage amplification mode, the sensor in response to •OH exhibited remarkable fluorescence enhancement (near 400-fold) in cell lysates, and the •OH was linearly determined from 0 to 800 nM with a detection limit of 6.4 pM. Moreover, this sensor can track basal level and fluctuation of •OH in living cells on account of its high sensitivity. To our knowledge, this is the first effort to use cytoplasmic protein for amplifying detection signals, which will provide a new dimension to current methodologies for low-abundance biomarkers discovery and regulation for chemical biology and medical diagnostics.
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- 2019
24. Frontispiz: A Glucose‐Powered Activatable Nanozyme Breaking pH and H 2 O 2 Limitations for Treating Diabetic Infections
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Lifang Chen, Shuohui Xing, Yanli Lei, Qiaoshu Chen, Zhen Zou, Ke Quan, Zhihe Qing, Juewen Liu, and Ronghua Yang
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General Medicine - Published
- 2021
25. Carbon dots for all-in-one detection and degradation: The role of photoinduced electron transfer
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Jiaoyan Huang, Caicheng Long, Lei Zhang, Taiping Qing, Peng Zhang, Zhihe Qing, and Bo Feng
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Process Chemistry and Technology ,Chemical Engineering (miscellaneous) ,Pollution ,Waste Management and Disposal - Published
- 2022
26. Zn
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Zhen, Zou, Libei, He, Xiangxi, Deng, Huangxiang, Wang, Ziyun, Huang, Qian, Xue, Zhihe, Qing, Yanli, Lei, Ronghua, Yang, and Juewen, Liu
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Microscopy, Confocal ,Cell Survival ,Gene Transfer Techniques ,Carbocyanines ,MicroRNAs ,Zinc ,Nanomedicine ,Microscopy, Electron, Transmission ,Coordination Complexes ,Cell Line, Tumor ,Humans ,Nanoparticles ,RNA ,RNA, Messenger ,Particle Size - Abstract
Metal-coordination-directed biomolecule crosslinking in nature has been used for synthesizing various biopolymers, including DNA, peptides, proteins, and polysaccharides. However, the RNA biopolymer has been avoided so far, as due to the poor stability of the RNA molecules, the formation of a biopolymer may alter the biological function of the molecules. Herein, for the first time, we report Zn
- Published
- 2021
27. A Glucose-Powered Activatable Nanozyme Breaking pH and H
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Lifang, Chen, Shuohui, Xing, Yanli, Lei, Qiaoshu, Chen, Zhen, Zou, Ke, Quan, Zhihe, Qing, Juewen, Liu, and Ronghua, Yang
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Glucose Oxidase ,Mice ,Glucose ,Hydroxyl Radical ,Diabetes Mellitus ,Animals ,Hydrogen Peroxide ,Hydrogen-Ion Concentration ,Staphylococcal Infections ,Anti-Bacterial Agents - Abstract
The peroxidase-like activity of nanozymes is promising for chemodynamic therapy by catalyzing H
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- 2021
28. Protonation-induced DNA conformational-change dominated electrochemical platform for glucose oxidase and urease analysis
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Dandan Hu, Di Wu, Yanmei Lu, Jiayue Liu, Zhiyong Guo, Sui Wang, Chunyang Zhai, Zhihe Qing, and Yufang Hu
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Cytosine ,Glucose Oxidase ,Glucose ,Environmental Chemistry ,Biosensing Techniques ,Electrochemical Techniques ,Urease ,Biochemistry ,Spectroscopy ,Analytical Chemistry - Abstract
Cytosine and protonated cytosine base pairs (C·CH
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- 2022
29. An intramolecular catalytic hairpin assembly on a DNA tetrahedron for mRNA imaging in living cells: improving reaction kinetics and signal stability
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Taiping Qing, Zhihe Qing, Jinlei Hu, Yanli Lei, Jingyuan Xu, Zhen Zou, and Ronghua Yang
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Chemical kinetics ,Reaction rate ,chemistry.chemical_compound ,Chemistry ,Förster resonance energy transfer ,Intramolecular force ,Kinetics ,Biophysics ,A-DNA ,General Chemistry ,Transfection ,DNA - Abstract
Enzyme-free amplification techniques based on dynamic DNA self-assembly (DDSA) have recently been developed for the in situ detection of mRNA in living cells. However, signal generation in traditional DDSA amplifiers is mainly dependent on the random diffusion of dissociative probes in a bulk solution, which is generally accompanied by poor kinetics and interference from complex biological systems. In this work, a new amplifier based on the design of an intramolecular catalytic hairpin assembly (intra-CHA) is proposed for the FRET imaging of mRNA in living cells. Compared with that in the free catalytic hairpin assembly (free-CHA), probes H1 and H2 in intra-CHA were simultaneously fixed on a DNA tetrahedron. The distance between them was closer, the local concentration of H1 and H2 in intra-CHA was theoretically approximately 808-times higher than that in free-CHA, and the initial reaction rate was enhanced 15.6 fold. Due to the spatial confinement effect, the reaction kinetics for target-catalyzed signal generation were significantly improved. By virtue of the three-dimensional nanostructure, H1 and H2 in the intra-CHA amplifier entered cells without any transfection or nanocarrier, and the probes and their products were free from biological interference, providing much higher signal stability for the reliable imaging of mRNA in living cells., An intramolecular catalytic hairpin assembly is implemented on a DNA tetrahedron for mRNA imaging in living cells. The spatial confinement effect enables the acceleration of target-triggered signal generation, with excellent cell permeability and FRET signal stability.
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- 2021
30. In Situ Amplification‐Based Imaging of RNA in Living Cells
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Jingyuan Xu, Sheng Yang, Zhen Zou, Weihong Tan, Jinlei Hu, Lei He, Ronghua Yang, Zhihe Qing, and Jing Zheng
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In situ ,010405 organic chemistry ,business.industry ,Loop-mediated isothermal amplification ,RNA ,General Chemistry ,Biology ,010402 general chemistry ,01 natural sciences ,Catalysis ,Molecular Imaging ,0104 chemical sciences ,Cell biology ,In vivo ,RNA analysis ,Nucleic acid ,Humans ,business ,Nucleic Acid Amplification Techniques ,Biomedicine ,Preclinical imaging - Abstract
Owing to its important physiological functions, especially as molecular biomarkers of diseases, RNA is an important focus of biomedicine and biochemical sensing. Signal amplification detection has been put forward because of the need for accurate identification of RNA at low expression levels, which is significant for the early diagnosis and therapy of malignant diseases. However, conventional amplification methods for RNA analysis depend on the use of enzymes, fixation of cells, and thermal cycling, which confine their performance to cell lysates or dead cells, thus the imaging of RNA in living cells remained until recently little explored. In recent years, the advance of isothermal amplification of nucleic acids has opened paths for meeting this need in living cells. This minireview tracks the development of in situ amplification assays for RNAs in living cells, and highlights the potential challenges facing this field, aiming to improve the development of in vivo isothermal amplification as well as usher in new frontiers in this fertile research area.
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- 2019
31. In Situ Amplification‐Based Imaging of RNA in Living Cells
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Zhihe Qing, Jingyuan Xu, Jinlei Hu, Jing Zheng, Lei He, Zhen Zou, Sheng Yang, Weihong Tan, and Ronghua Yang
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General Medicine - Published
- 2019
32. MIL/Aptamer as a Nanosensor Capable of Resisting Nonspecific Displacement for ATP Imaging in Living Cells
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Yuedong Zhang, Zhihe Qing, Jianxiao Yang, Feng Feng, Lihua Zhang, Zhen Zou, Ronghua Yang, Sheng Yang, and Jun Li
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Quenching (fluorescence) ,Chemistry ,General Chemical Engineering ,Aptamer ,High selectivity ,Nanotechnology ,General Chemistry ,Fluorescence ,Article ,Nanomaterials ,lcsh:Chemistry ,lcsh:QD1-999 ,Nanosensor ,Drug delivery ,Intracellular - Abstract
Fluorescent probes physisorbed on nanomaterials have emerged as a kind of useful and facile sensing platform for biological important molecules. However, nonspecific displacement in the physisorption systems is a non-negligible problem for the intracellular analysis. MIL (Materials of Institut Lavoisier), a subclass of metal–organic frameworks (MOFs), has high porosity, large surface area, and intriguing three-dimensional (3D) nanostructure with promising biological and biomedical applications such as molecular detection and drug delivery. Herein, we report MIL/aptamer-FAM as a nanosensor capable of resisting nonspecific displacement for intracellular adenosinetriphosphate (ATP) sensing and imaging. In this approach, by virtue of the remarkable quenching capability, high affinity of aptamers, and dramatic capability of resisting nonspecific displacement of 3D MIL-100, the assay and imaging of ATP in living cells were realized. Our results demonstrated that the MIL/aptamer-FAM nanosensor not only shows high selectivity for the detection of ATP in buffer but also is able to act as a “signal-on” nanosensor for specific imaging of ATP in living cells. The strategy reported here opens up a new way to develop MOF-based nanosensors for intracellular delivery and metabolite detection.
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- 2019
33. Synchronous screening of multiplexed biomarkers of Alzheimer's disease by a length-encoded aerolysin nanopore-integrated triple-helix molecular switch
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Ronghua Yang, Peng Qi, Lihua Zhang, Zhihe Qing, Xuan Xu, Feng Feng, Hua Yang, Jing Zheng, Zhen Zou, and Qi Yan
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Pore Forming Cytotoxic Proteins ,Molecular switch ,010405 organic chemistry ,Oligonucleotide ,Chemistry ,Bacterial Toxins ,Metals and Alloys ,Aerolysin ,General Chemistry ,Computational biology ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Nanopores ,Nanopore ,Alzheimer Disease ,Materials Chemistry ,Ceramics and Composites ,Humans ,Signal transduction ,Biomarkers ,Triple helix - Abstract
Herein, we developed a versatile strategy for the synchronous screening of biomarkers of Alzheimer's disease (AD) by incorporating the high-resolution discrimination of an aerolysin nanopore to length-encoded oligonucleotides and the signal transduction of a triple-helix molecular switch (TMS). This assay is adapted to quantitatively compare multiplexed targets of very different native contents without tedious chemical labelling.
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- 2019
34. A dsDNA-lighted fluorophore for monitoring protein-ligand interaction through binding-mediated DNA protection
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Ronghua Yang, Zhen Zou, Lina Hou, Zhihe Qing, Lixuan Zhu, and Sheng Yang
- Subjects
Exonuclease III ,DNA protection ,Fluorophore ,biology ,Hybridization probe ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,biology.protein ,Biophysics ,Target protein ,0210 nano-technology ,DNA ,Protein ligand - Abstract
Because of their important roles in cellular functions, life activities, drug screening, and disease treatment, the development of efficient methods for monitoring protein-ligand interaction is essential. In this study, inspired by our previous studies on DNA conformation-selective fluorescent indicators, we developed a new sensing platform for monitoring protein-ligand interaction and detecting protein activity based on binding-mediated DNA protection and the dsDNA-lighted fluorophore, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9 H -carbazol-9-yl)] butanoate (EBCB). The ligand was purposefully linked to the 3ʹ-terminal of a hairpin DNA probe to selectively bind with the target protein and protect the DNA from cleavage by exonuclease III. By virtue of EBCB’s outstanding capacity to discriminate DNA conformation, the protein-ligand interaction could be effectively monitored through a fluorescence change in EBCB. A high fluorescence signal was detected when the hairpin DNA was protected in the presence of the target protein, whereas a much lower signal was observed in the presence of nontarget proteins. Our results demonstrated that the proposed strategy had high potential, such as high selectivity and relatively high sensitivity, for monitoring protein-ligand interaction and detecting protein activity. We believe these results will pave the way for applying dsDNA-lighted fluorophore EBCB as an effective signal transducer for DNA conformation transformation-mediated biochemical sensing.
- Published
- 2018
35. Adsorption-improved MoSe
- Author
-
Caicheng, Long, Xiao, Li, Zixin, Jiang, Peng, Zhang, Zhihe, Qing, Taiping, Qing, and Bo, Feng
- Abstract
Water pollution arising from heavy metal ions continues to be a major environmental problem, which represents a serious threat to human beings and animals worldwide. New materials that can simultaneously detect and remove these toxic ions are urgently required. Herein, nitrogen and sulfur co-doped molybdenum selenide nanosheets (N, S-MoSe
- Published
- 2020
36. Thiol-suppressed I2-etching of AuNRs: acetylcholinesterase-mediated colorimetric detection of organophosphorus pesticides
- Author
-
Zhen Zou, Yacheng Li, Ronghua Yang, Guoyan Luo, Jinlei Hu, Younan Li, Zhihe Qing, Yanli Lei, and Juewen Liu
- Subjects
Detection limit ,chemistry.chemical_classification ,010401 analytical chemistry ,Nanochemistry ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Combinatorial chemistry ,Acetylcholinesterase ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Thiocholine ,chemistry ,Etching (microfabrication) ,Thiol ,Nanorod ,0210 nano-technology ,Organophosphorus pesticides - Abstract
For the first time it is demonstrated that sulfhydryl compounds can suppress longitudinal etching of gold nanorods via consuming oxidizers, which provides a new signaling mechanism for colorimetric sensing. As a proof of concept, a colorimetric assay is developed for detecting organophosphorus pesticides, which are most widely used in modern agriculture to improve food production but with high toxicity to animals and the ecological environment. Triazophos was selected as a model organophosphorus pesticide. In the absence of triazophos, the active acetylcholinesterase can catalyze the conversion of acetylthiocholine iodide to thiocholine whose thiol group can suppress the I2-induced etching of gold nanorods. When triazophos is present, the activity of AchE is inhibited, and I2-induced etching of gold nanorods results in triazophos concentration-dependent color change from brown to blue, pink, and red. The aspect ratio of gold nanorods reduced with gradually blue-shifted longitudinal absorption. There was a linear detection range from 0 to 117 nM (R2 = 0.9908), the detection limit was 4.69 nM, and a good application potential was demonstrated by the assay of real water samples. This method will not only contribute to public monitoring of organophosphorus pesticides but also has verified a new signaling mechanism which will open up a new path to develop colorimetric detection methods.
- Published
- 2020
37. Pt-S Bond-Mediated Nanoflares for High-Fidelity Intracellular Applications by Avoiding Thiol Cleavage
- Author
-
Ronghua Yang, Zhen Zou, Yanli Lei, Guoyan Luo, Zhihe Qing, Shuohui Xing, and Juewen Liu
- Subjects
Aptamer ,chemistry.chemical_element ,Metal Nanoparticles ,010402 general chemistry ,Cleavage (embryo) ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Microscopy, Electron, Transmission ,Humans ,Sulfhydryl Compounds ,Fluorescent Dyes ,Platinum ,chemistry.chemical_classification ,010405 organic chemistry ,Ligand ,Selenol ,General Chemistry ,Combinatorial chemistry ,0104 chemical sciences ,Nanostructures ,chemistry ,Microscopy, Fluorescence ,Colloidal gold ,Thiol ,MCF-7 Cells ,Gold ,Biosensor ,Sulfur - Abstract
The Au-S bond is the classic way to functionalize gold nanoparticles (AuNPs). However, cleavage of the bond by biothiols and other chemicals is a long-standing problem hindering practical applications, especially in cells. Instead of replacing the thiol by a carbene or selenol for stronger adsorption, it is now shown that the Pt-S bond is much more stable, fully avoiding cleavage by biothiols. AuNPs were deposited with a thin layer of platinum, and an AuNP@Pt-S nanoflare was constructed to detect the miRNA-21 microRNA in living cells. This design retained the optical and cellular uptake properties of DNA-functionalized AuNPs, while showing high-fidelity signaling. It discriminated target cancer cells even in a mixed-cell culture system, where the Au-S based nanoflare was less sensitive. Compared to previous methods of changing the ligand chemistry, coating a Pt shell is more accessible, and previously developed methods for AuNPs can be directly adapted.
- Published
- 2020
38. Thiol-suppressed I
- Author
-
Zhihe, Qing, Yacheng, Li, Younan, Li, Guoyan, Luo, Jinlei, Hu, Zhen, Zou, Yanli, Lei, Juewen, Liu, and Ronghua, Yang
- Subjects
Nanotubes ,Drinking Water ,Organothiophosphates ,Triazoles ,Proof of Concept Study ,Lakes ,Acetylthiocholine ,Limit of Detection ,Acetylcholinesterase ,Colorimetry ,Cholinesterase Inhibitors ,Gold ,Sulfhydryl Compounds ,Pesticides ,Water Pollutants, Chemical ,Iodine - Abstract
For the first time it is demonstrated that sulfhydryl compounds can suppress longitudinal etching of gold nanorods via consuming oxidizers, which provides a new signaling mechanism for colorimetric sensing. As a proof of concept, a colorimetric assay is developed for detecting organophosphorus pesticides, which are most widely used in modern agriculture to improve food production but with high toxicity to animals and the ecological environment. Triazophos was selected as a model organophosphorus pesticide. In the absence of triazophos, the active acetylcholinesterase can catalyze the conversion of acetylthiocholine iodide to thiocholine whose thiol group can suppress the I
- Published
- 2020
39. DNA-Templated Fluorescent Nanoclusters for Metal Ions Detection
- Author
-
Ying Chen, Liangliang Zhang, Ying Lu, Zhihe Qing, Chunxia Song, and Jingyuan Xu
- Subjects
DNA Replication ,Materials science ,Biocompatibility ,Aptamer ,Metal ions in aqueous solution ,nanoclusters ,detection ,Pharmaceutical Science ,Nanotechnology ,Biosensing Techniques ,Review ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,behavioral disciplines and activities ,Analytical Chemistry ,Nanoclusters ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Drug Discovery ,mental disorders ,Physical and Theoretical Chemistry ,Ions ,Molecular Structure ,Organic Chemistry ,metal ions ,DNA ,dna-template ,021001 nanoscience & nanotechnology ,Fluorescence ,Nanostructures ,0104 chemical sciences ,Template ,chemistry ,Metals ,Chemistry (miscellaneous) ,Molecular Medicine ,Surface modification ,fluorescence ,0210 nano-technology - Abstract
DNA-templated fluorescent nanoclusters (NCs) have attracted increasing research interest on account of their prominent features, such as DNA sequence-dependent fluorescence, easy functionalization, wide availability, water solubility, and excellent biocompatibility. Coupling DNA templates with complementary DNA, aptamers, G-quadruplex, and so on has generated a large number of sensors. Additionally, the preparation and applications of DNA-templated fluorescent NCs in these sensing have been widely studied. This review firstly focuses on the properties of DNA-templated fluorescent NCs, and the synthesis of DNA-templated fluorescent NCs with different metals is then discussed. In the third part, we mainly introduce the applications of DNA-templated fluorescent NCs for sensing metal ions. At last, we further discuss the future perspectives of DNA-templated fluorescent NCs in the synthesis and sensing metal ions in the environmental and biological fields.
- Published
- 2019
40. Technologies for analysis of circulating tumour DNA: Progress and promise
- Author
-
Weiju Chen, Sheng Yang, Jing Zheng, Ronghua Yang, Zhihe Qing, Zhen Zou, and Peng Qi
- Subjects
0301 basic medicine ,business.industry ,Cancer ,Nanotechnology ,Computational biology ,Gene mutation ,medicine.disease ,Peripheral blood ,Analytical Chemistry ,Biomarker (cell) ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Molecular level ,030220 oncology & carcinogenesis ,Medicine ,Liquid biopsy ,business ,Spectroscopy - Abstract
Circulating tumour DNA (ctDNA) isolated from peripheral blood has recently been shown to be a biomarker to detect gene mutations for the diagnosis, treatment, and prognosis of cancer. Utilizing ctDNA as the liquid biopsy has significant potential to pave the way toward a better understanding of cancer at the molecular level and improve patient outcomes in the future. Over the past decades, a large number of efforts have been devoted to the development of valid methods for analysing ctDNA, which provide researchers and clinicians a variety of tools to detect and monitor tumours. In this review, we displayed an overview of current representative technologies for the detection of ctDNA and discuss recent technical advancements. Then, the challenges and outlooks in this promising field are featured on the basis of its current development.
- Published
- 2017
41. A Target-Lighted dsDNA-Indicator for High-Performance Monitoring of Mercury Pollution and Its Antagonists Screening
- Author
-
Zhen Zou, Jingru Guo, Zhihe Qing, Sheng Yang, Zhong Cao, Lixuan Zhu, Xiaoxuan Li, and Ronghua Yang
- Subjects
010405 organic chemistry ,Drinking Water ,Metal ions in aqueous solution ,Mercury pollution ,Hybridization probe ,chemistry.chemical_element ,Biosensing Techniques ,DNA ,Mercury ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Mercury (element) ,chemistry.chemical_compound ,chemistry ,Bioaccumulation ,Environmental chemistry ,Nucleic acid ,Humans ,Environmental Chemistry ,DNA Probes - Abstract
As well-known, the excessive discharge of heavy-metal mercury not only destroys the ecological environment, bust also leads to severe damage of human health after ingestion via drinking and bioaccumulation of food chains, and mercury ion (Hg2+) is designated as one of most prevalent toxic metal ions in drinking water. Thus, the high-performance monitoring of mercury pollution is necessary. Functional nucleic acids have been widely used as recognition probes in biochemical sensing. In this work, a carbazole derivative, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9H-carbazol −9-yl)] butanoate (EBCB), has been synthesized and found as a target-lighted DNA fluorescent indicator. As a proof-of-concept, Hg2+ detection was carried out based on EBCB and Hg2+-mediated conformation transformation of a designed DNA probe. By comparison with conventional nucleic acid indicators, EBCB held excellent advantages, such as minimal background interference and maximal sensitivity. Outstanding detection capabilities we...
- Published
- 2017
42. Adsorption-improved MoSe2 nanosheet by heteroatom doping and its application for simultaneous detection and removal of mercury (II)
- Author
-
Xiao Li, Zhihe Qing, Bo Feng, Zixin Jiang, Peng Zhang, Taiping Qing, and Caicheng Long
- Subjects
021110 strategic, defence & security studies ,Environmental Engineering ,Aqueous solution ,Health, Toxicology and Mutagenesis ,Metal ions in aqueous solution ,Inorganic chemistry ,Heteroatom ,0211 other engineering and technologies ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Pollution ,Mercury (element) ,chemistry.chemical_compound ,Adsorption ,chemistry ,Molybdenum ,Selenide ,Environmental Chemistry ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Nanosheet - Abstract
Water pollution arising from heavy metal ions continues to be a major environmental problem, which represents a serious threat to human beings and animals worldwide. New materials that can simultaneously detect and remove these toxic ions are urgently required. Herein, nitrogen and sulfur co-doped molybdenum selenide nanosheets (N, S-MoSe2) were prepared and found to be fluorescently responsive to mercury (II) with an improved adsorption capacity (208.33 mg g−1), thereby providing the possibility for the simultaneous detection and removal of mercury (II) in water samples. The great affinity was the result of the complexation of mercury (II) with Se and S atoms in N, S-MoSe2 as well as the electrostatic adsorption of cation mercury (II) on negatively charged N, S-MoSe2. Besides good sensitivity and selectivity toward mercury (II), N, S-MoSe2 displayed a relatively consistent performance under a wide pH range from 3 to 10. The removal efficiency reached 87.5% with fast adsorption kinetics, and N, S-MoSe2 could be reused after simple treatment. Thus, this work is expected to provide new material for the detection and removal of mercury (II) in an aqueous solution and offer an insight into the interaction between heavy metal ions and inorganic nanomaterials.
- Published
- 2021
43. Ratiometric Visualization of NO/H2S Cross-Talk in Living Cells and Tissues Using a Nitroxyl-Responsive Two-Photon Fluorescence Probe
- Author
-
Zhihe Qing, Ronghua Yang, Jing Zheng, Yibo Zhou, Sheng Yang, Xiufang Zhang, Jishan Li, and Yuan Li
- Subjects
010405 organic chemistry ,Chemistry ,Nitroxyl ,010402 general chemistry ,Two photon fluorescence ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Analytical Chemistry ,Crosstalk ,chemistry.chemical_compound ,Förster resonance energy transfer ,Biochemistry ,Biophysics ,Moiety ,Molecular imaging ,Gasotransmitters - Abstract
It is of scientific significance to explore the intricate relationship between two crucial gasotransmitters nitric oxide (NO) and hydrogen sulfide (H2S) because they exert similar and interdependent biological actions within the living organisms. Nevertheless, visualization of the NO/H2S crosstalk using effective molecular imaging tools remains challenging. To address this issue, and given that nitroxyl (HNO) has been implicated as the interdependent production of NO and H2S via a network of cascading chemical reactions, we herein design a ratiometric two-photon fluorescent probe for HNO, termed TP-Rho-HNO, which consists of benzo[h]chromene-rhodol scaffold as two-photon energy transfer cassette with phosphine moiety as specific HNO recognition unit. The newly proposed probe has been successfully applied in ratiometric two-photon bioimaging of endogenous HNO derived from NO and H2S interaction in the human umbilical vein cells (HUVECs) and as well as in rat brain tissues. Intriguingly, the imaging results...
- Published
- 2017
44. Oligonucleotide-templated rapid formation of fluorescent gold nanoclusters and its application for Hg2+ ions sensing
- Author
-
Zhihe Qing, Kemin Wang, Yanli Lei, Yue Li, Peng Tang, Xiaoxiao He, Dinggeng He, Ting Liu, and Taiping Qing
- Subjects
Detection limit ,Oligonucleotide ,Metal ions in aqueous solution ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Combinatorial chemistry ,Fluorescence ,0104 chemical sciences ,Analytical Chemistry ,Nanoclusters ,chemistry.chemical_compound ,Template ,chemistry ,0210 nano-technology ,Selectivity ,DNA - Abstract
In this work, we developed a simple, rapid and mild strategy for synthesis of DNA-templated fluorescent gold nanoclusters (AuNCs) through association of gold ions to DNA templates and reduction with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). After systematical investigation on the formation of fluorescent AuNCs by using different DNA templates, C5 DNA was found as the best template for the formation of fluorescent AuNCs in this system. This process could be completed within 5min after the reaction beginning under ambient conditions. The prepared C5-AuNCs displayed good blue emission and photostability. Furthermore, this C5-AuNCs could be applied to detecting Hg2+ ions specifically based on the specific and strong interaction between Hg2+ and Au+. The C5-AuNCs provided excellent selectivity for Hg2+ ions over other metal ions, and also high sensitivity, with a detection limit of 50nM. As biocompatible, environmentally-friendly, and synthesis-rapid, this C5-AuNCs probe appears to be promising candidate for biochemical sensing via simple modification of template DNA.
- Published
- 2016
45. Recent progress in copper nanocluster-based fluorescent probing: a review
- Author
-
Taiping Qing, Xuan Wang, Peng Zhang, Bo Feng, Caicheng Long, Zhihe Qing, Haizhi Hu, and Kaiwu Zhang
- Subjects
chemistry.chemical_classification ,Chemistry ,Biomolecule ,Metal ions in aqueous solution ,Nanoprobe ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Small molecule ,Work related ,Fluorescence spectroscopy ,0104 chemical sciences ,Analytical Chemistry ,Nanoclusters ,Molecule ,0210 nano-technology - Abstract
Copper nanoclusters (CuNCs) are an attractive alternative to other metal nanoclusters. The synthesis of CuNCs is highly efficient and fast, with low-cost and without any complicated manipulation. Because of their tunable fluorescence and low toxicity, CuNCs have been highly exploited for biochemical sensing. This review (with 172 refs.) summarizes the progress that has been made in the field in the past years. Following an introduction into the fundamentals of CuNCs, the review first focuses on synthetic methods and the fluorescence properties of CuNCs (with subsections on the use of proteins, peptides, DNA and other molecules as templates). This is followed by a section on the use of CuNCs in fluorometric assays, with subsections on the detection of small molecules, proteins, nucleic acids, various other biomolecules including drugs, and of pH values. A further large chapter summarizes the work related to environmental analyses, specifically on determination of metal ions, anions and pollutants. Graphical abstract Schematic representation of the synthesis and potential applications of copper nanocluster (CuNCs) in biochemical analysis, emphatically reflected in some vital areas such as small molecule analysis, biomacromolecule monitoring, cell imaging, ions detection, toxic pollutant, etc.
- Published
- 2019
46. Real-Time Visualizing Mitophagy-Specific Viscosity Dynamic by Mitochondria-Anchored Molecular Rotor
- Author
-
Zhen Zou, Peng Qi, Feng Feng, Sixin Ai, Zhihe Qing, Yufei Zhang, Lihua Zhang, Qi Yan, Hua Yang, and Ronghua Yang
- Subjects
Intrinsic viscosity ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Analytical Chemistry ,law.invention ,Viscosity ,law ,Mitophagy ,Humans ,Fluorescent Dyes ,Cellular process ,Chemistry ,Rotor (electric) ,010401 analytical chemistry ,Optical Imaging ,Viscometer ,0104 chemical sciences ,Mitochondria ,HEK293 Cells ,Spectrometry, Fluorescence ,Biophysics ,MCF-7 Cells ,HeLa Cells - Abstract
Mitophagy, as an evolutionarily conserved cellular process, plays a crucial role in preserving cellular metabolism and physiology. Various microenvironment alterations assigned to mitophagy including pH, polarity, and deregulated biomarkers are increasingly understood. However, mitophagy-specific viscosity dynamic in live cells remains a mystery and needs to be explored. Here, a water-soluble mitochondria-targetable molecular rotor, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9 H-carbazol-9-yl)] butanoate (BMVC), was exploited as a fluorescent viscosimeter for imaging viscosity variation during mitophagy. This probe contains two positively charged 1-methyl-4-vinylpyridium components as the rotors, whose rotation will be hindered with the increase of environmental viscosity, resulting in enhancement of fluorescence emission. The results demonstrated that this probe operates well in a mitochondrial microenvironment and displays an off-on fluorescence response to viscosity. By virtue of this probe, new discoveries such as the mitochondrial viscosity will increase during mitophagy are elaborated. The real-time visualization of the mitophagy process under nutrient starvation conditions was also proposed and actualized. We expect this probe would be a robust tool in the pathogenic mechanism research of mitochondrial diseases.
- Published
- 2019
47. Cell-Surface-Anchored Ratiometric DNA Nanoswitch for Extracellular ATP Imaging
- Author
-
Shian Zhong, Xiufang Li, Yanjing Yang, Jianbing Li, Hui Liu, Jing Yuan, Yao He, Zhihe Qing, and Zhiwei Deng
- Subjects
Fluid Flow and Transfer Processes ,Chemistry ,Process Chemistry and Technology ,Aptamer ,010401 analytical chemistry ,Cell ,Bioengineering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Cell membrane ,Closed state ,chemistry.chemical_compound ,medicine.anatomical_structure ,Förster resonance energy transfer ,medicine ,Biophysics ,Extracellular ,A-DNA ,0210 nano-technology ,Instrumentation ,DNA - Abstract
The precise detection of extracellular ATP, although a challenging task, is of great significance for understanding the related cell processes. Herein, we developed a ratiometric DNA nanoswitch by employing a DNA tweezer and split aptamer. The nanoswitch is composed of three specially designed ssDNA strands, namely, the central strands O1, O2, and O3. This nanoswitch can be anchored on the cell membrane by cholesterol labeled at the 3' end of O3. Initially, the DNA tweezer adopts an open state, separating the dual fluorophores and giving rise to a low FRET (fluorescence resonance energy transfer) ratio. The presence of ATP induces the binding of the two split aptamers to alter the structure of the nanoswitch from the open state to the closed state, bringing the donor and the acceptor closer together and generating high FRET efficiency. The results demonstrated that the ratiometric DNA nanoswitch can be applied for quantitative analysis and real-time monitoring of the changes in extracellular ATP. We believe that the cell surface-anchored DNA nanoswitch has promising prospects for use as a powerful tool for the understanding of different ATP-related physiological activities.
- Published
- 2019
48. Progress in biosensor based on DNA-templated copper nanoparticles
- Author
-
Ailing Bai, Shuohui Xing, Kemin Wang, Zhihe Qing, Zhen Zou, Ronghua Yang, and Xiaoxiao He
- Subjects
Materials science ,Biomedical Engineering ,Biophysics ,Nanoparticle ,Metal Nanoparticles ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Fluorescence ,Nanomaterials ,Molecular recognition ,Electrochemistry ,Microelectronics ,Surface plasmon resonance ,Inductively coupled plasma mass spectrometry ,Fluorescent Dyes ,business.industry ,010401 analytical chemistry ,General Medicine ,DNA ,Surface-enhanced Raman spectroscopy ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Spectrometry, Fluorescence ,0210 nano-technology ,business ,Biosensor ,Copper ,Biotechnology - Abstract
During the last decades, by virtue of their unique physicochemical properties and potential application in microelectronics, biosensing and biomedicine, metal nanomaterials (MNs) have attracted great research interest and been highly developed. Deoxyribonucleic acid (DNA) is a particularly interesting ligand for templating bottom-up nanopreparation, by virtue of its excellent properties including nanosized geometry structure, programmable and artificial synthesis, DNA-metal ion interaction and powerful molecular recognition. DNA-templated copper nanoparticles (DNA-CuNPs) has been developed in recent years. Because of its advantages including simple and rapid preparation, high efficiency, MegaStokes shifting and low biological toxicity, DNA-CuNPs has been highly exploited for biochemical sensing from 2010, especially as a label-free detection manner, holding advantages in multiple analytical technologies including fluorescence, electrochemistry, surface plasmon resonance, inductively coupled plasma mass spectrometry and surface enhanced Raman spectroscopy. This review comprehensively tracks the preparation of DNA-CuNPs and its application in biosensing, and highlights the potential development and challenges regarding this field, aiming to promote the advance of this fertile research area.
- Published
- 2019
49. Colorimetric aminotriazole assay based on catalase deactivation-dependent longitudinal etching of gold nanorods
- Author
-
Guoyan Luo, Ronghua Yang, Xiaoxuan Li, Zhen Zou, Zhihe Qing, and Younan Li
- Subjects
genetic structures ,Iodide ,chemistry.chemical_element ,Nanochemistry ,Color ,Food Contamination ,02 engineering and technology ,010402 general chemistry ,Iodine ,01 natural sciences ,Analytical Chemistry ,Etching (microfabrication) ,Limit of Detection ,Amitrole ,Detection limit ,chemistry.chemical_classification ,Nanotubes ,biology ,Herbicides ,Hydrogen Peroxide ,021001 nanoscience & nanotechnology ,Catalase ,0104 chemical sciences ,chemistry ,Color changes ,biology.protein ,Nanorod ,Colorimetry ,sense organs ,Gold ,0210 nano-technology ,Water Pollutants, Chemical ,Nuclear chemistry - Abstract
A colorimetric and visual assay is described for the herbicide aminotriazole (ATZ). It is based on the etching of gold nanorods (AuNRs) by iodine which is formed on oxidation of iodide via H2O2. Longitudinal etching of the AuNRs occurs quickly and is accompanied by a color change from dark blue to red. In the absence of ATZ and the presence of active catalase (CAT), H2O2 is quickly decomposed into water, and the AuNRs will not be etched. In the presence of ATZ, CAT is partially deactivated, and this affects the amount of available H2O2 and, consequently, of the iodine. Hence, the color is significantly changed. The color changes can be easily detected with bare eyes. The assay has a linear response in the 5 to 70 μM concentration range, with a detection limit of 1.3 μM and high selectivity for ATZ. It was applied to the determination of ATZ in water and food samples.
- Published
- 2019
50. A Ratiometric Two-Photon Fluorescent Cysteine Probe with Well-Resolved Dual Emissions Based on Intramolecular Charge Transfer-Mediated Two-Photon-FRET Integration Mechanism
- Author
-
Jingru Guo, Sheng Yang, Yuan Li, Zhihe Qing, Ronghua Yang, Jiangsheng Li, Jie Xiao, and Chongchong Guo
- Subjects
Fluorescence-lifetime imaging microscopy ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Rats, Sprague-Dawley ,Two-photon excitation microscopy ,Coumarins ,Fluorescence Resonance Energy Transfer ,Animals ,Humans ,Emission spectrum ,Cysteine ,Absorption (electromagnetic radiation) ,Instrumentation ,Fluorescent Dyes ,Fluid Flow and Transfer Processes ,Oxadiazoles ,Photons ,Chemistry ,Process Chemistry and Technology ,021001 nanoscience & nanotechnology ,Fluorescence ,0104 chemical sciences ,Förster resonance energy transfer ,Liver ,Excited state ,Intramolecular force ,0210 nano-technology ,HeLa Cells - Abstract
The development of an efficient ratiometric two-photon fluorescence imaging probe is crucial for in situ monitoring of biothiol cysteine (Cys) in biosystems, but the current reported intramolecular charge transfer (ICT)-based one suffers from serious overlap between the shifted emission bands. To address this issue, we herein for the first time constructed an ICT-mediated two-photon excited fluorescence resonance energy transfer (TP-FRET) system consisting of a two-photon fluorogen benzo[ h]chromene and a Cys-responsive benzoxadiazole-analogue dye. Different from a previous mechanism that utilized single two-photon fluorogen to acquire a ratiometric signal, ICT was used to switch on the TP-FRET process of the energy transfer dyad by eliciting an absorption shift of benzoxadiazole with Cys to modulate the spectral overlap level between benzo[ h]chromene emission and benzoxadiazole absorption, resulting in two well-separated emission signal changes with large emission wavelength shift (120 nm), fixed two-photon excitation maximum (750 nm), and significant variation in fluorescence ratio (over 36-fold). Therefore, it can be successfully employed to ratiometrically visualize Cys in HeLa cells and liver tissues. Importantly, this new ICT-mediated TP-FRET integration mechanism would be convenient for designing ratiometric two-photon fluorescent probes with two well-resolved emission spectra suitable for high resolution two-photon fluorescence bioimaging.
- Published
- 2018
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