1. Elevated expression of thyroid hormone receptor α 2 (c-erb A- α 2) in nasopharyngeal carcinoma
- Author
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Bi-Tzen Juang, C. S. Yang, Jun-Wei Chen, S. L. Doong, J. W. Lee, and C. L. Chen
- Subjects
Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,c-erb A-α2 (TR-α2) ,Molecular Sequence Data ,Biology ,Complementary DNA ,Sequence Homology, Nucleic Acid ,Gene expression ,Carcinoma ,medicine ,otorhinolaryngologic diseases ,Tumor Cells, Cultured ,Humans ,Northern blot ,RNA, Neoplasm ,In Situ Hybridization ,Aged ,Differential display ,Thyroid hormone receptor ,Receptors, Thyroid Hormone ,Base Sequence ,nasopharyngeal carcinoma ,Regular Article ,Nasopharyngeal Neoplasms ,DNA, Neoplasm ,Middle Aged ,medicine.disease ,Blotting, Northern ,Molecular biology ,Blot ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,Blotting, Southern ,Oncology ,Nasopharyngeal carcinoma ,Female ,differential display ,RNA in situ hybridization - Abstract
Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor α2 (TR-α2 or c-erb A-α2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-α2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-α2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-α2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-α2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-α2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P
- Published
- 2000