1. Thermopriming triggers splicing memory in Arabidopsis
- Author
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Yong H. Woo, Ge Gao, Natalia Serrano, Anireddy S. N. Reddy, Martin Crespi, Magdy M. Mahfouz, Morad M. Mokhtar, Moussa Benhamed, Alaguraj Veluchamy, Mohamed A. M. Atia, Christoph A Gehring, Jérémie Bazin, Yu Ling, cgcad, Thss, Tsinghua University [Beijing] (THU), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), King Abdullah University of Science and Technology (KAUST), Tsinghua University [Beijing], and Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,0301 basic medicine ,Hot Temperature ,Arabidopsis thaliana ,Physiology ,RNA Splicing ,[SDV]Life Sciences [q-bio] ,heat-stress priming ,Arabidopsis ,Plant Science ,Biology ,heat responses ,eXtra Botany ,intron retention ,01 natural sciences ,heat stress ,alternative splicing ,03 medical and health sciences ,Gene Expression Regulation, Plant ,heat-stress memory ,Gene expression ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Adaptation ,Heat shock ,stress memory ,ComputingMilieux_MISCELLANEOUS ,2. Zero hunger ,Regulation of gene expression ,heat priming ,fungi ,Alternative splicing ,food and beverages ,stress response ,biology.organism_classification ,Research Papers ,Cell biology ,030104 developmental biology ,Plant—Environment Interactions ,RNA splicing ,Acquired tolerance ,Transcriptome ,Insight ,Priming (psychology) ,Heat-Shock Response ,010606 plant biology & botany - Abstract
Thermopriming induces genome-wide differential gene expression and alternative splicing patterns, and establishes a ‘splicing memory’ that helps plants to survive subsequent and otherwise lethal heat stress., Abiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred to as priming, can allow plants to survive subsequent and otherwise lethal conditions; the priming effect persists even after a prolonged stress-free period. However, the molecular mechanisms underlying priming are not fully understood. Here, we investigated the molecular basis of heat-shock memory and the role of priming in Arabidopsis thaliana. Comprehensive analysis of transcriptome-wide changes in gene expression and alternative splicing in primed and non-primed plants revealed that alternative splicing functions as a novel component of heat-shock memory. We show that priming of plants with a non-lethal heat stress results in de-repression of splicing after a second exposure to heat stress. By contrast, non-primed plants showed significant repression of splicing. These observations link ‘splicing memory’ to the ability of plants to survive subsequent and otherwise lethal heat stress. This newly discovered priming-induced splicing memory may represent a general feature of heat-stress responses in plants and other organisms as many of the key components are conserved among eukaryotes. Furthermore, this finding could facilitate the development of novel approaches to improve plant survival under extreme heat stress.
- Published
- 2018
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