1. Timeline Kinetics of Systemic and Airway Immune Mediator Storm for Comprehensive Analysis of Disease Outcome in Critically Ill COVID-19 Patients.
- Author
-
Gonçalves JJ, da Mata CPSM, Lourenço AA, Ribeiro ÁL, Ferreira GM, Fraga-Silva TFC, de Souza FM, Almeida VES, Batista IA, D Avila-Mesquita C, Couto AES, Campos LCB, Paim AAO, Ferreira LL, de Melo Oliveira P, de Almeida Teixeira L, Priscila de Almeida Marques D, Retes de Moraes H, Pereira SH, Brito-de-Sousa JP, Campi-Azevedo AC, Peruhype-Magalhães V, Araújo MSS, Teixeira-Carvalho A, da Fonseca FG, Bonato VLD, Becari C, Ferro D, Menegueti MG, Mazzoni AAS, Auxiliadora-Martins M, Coelho-Dos-Reis JG, and Martins-Filho OA
- Subjects
- Critical Illness, Cytokines metabolism, Humans, Kinetics, SARS-CoV-2, COVID-19
- Abstract
In the present study, the levels of serum and airway soluble chemokines, pro-inflammatory/regulatory cytokines, and growth factors were quantified in critically ill COVID-19 patients (total n=286) at distinct time points (D0, D2-6, D7, D8-13 and D>14-36) upon Intensive Care Unit (ICU) admission. Augmented levels of soluble mediators were observed in serum from COVID-19 patients who progress to death. An opposite profile was observed in tracheal aspirate samples, indicating that systemic and airway microenvironment diverge in their inflammatory milieu. While a bimodal distribution was observed in the serum samples, a unimodal peak around D7 was found for most soluble mediators in tracheal aspirate samples. Systems biology tools further demonstrated that COVID-19 display distinct eccentric soluble mediator networks as compared to controls, with opposite profiles in serum and tracheal aspirates. Regardless the systemic-compartmentalized microenvironment, networks from patients progressing to death were linked to a pro-inflammatory/growth factor-rich, highly integrated center. Conversely, patients evolving to discharge exhibited networks of weak central architecture, with lower number of neighborhood connections and clusters of pro-inflammatory and regulatory cytokines. All in all, this investigation with robust sample size landed a comprehensive snapshot of the systemic and local divergencies composed of distinct immune responses driven by SARS-CoV-2 early on severe COVID-19., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Gonçalves, da Mata, Lourenço, Ribeiro, Ferreira, Fraga-Silva, de Souza, Almeida, Batista, D`Avila-Mesquita, Couto, Campos, Paim, Ferreira, de Melo Oliveira, de Almeida Teixeira, Priscila de Almeida Marques, Retes de Moraes, Pereira, Brito-de-Sousa, Campi-Azevedo, Peruhype-Magalhães, Araújo, Teixeira-Carvalho, da Fonseca, Bonato, Becari, Ferro, Menegueti, Mazzoni, Auxiliadora-Martins, Coelho-dos-Reis and Martins-Filho.)
- Published
- 2022
- Full Text
- View/download PDF