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The objective of this work was to evaluate the in vitro and ex vitro combination of P. chlamydosporia (IMI SD 187) and Trichoderma asperellum (Ta. 13) in the acclimatization of banana vitroplants Bluggoe type (AABB) ���FHIA-03���. Firstly, the in vitro compatibility, through space competence (radial growth) and growth inhibition percentage at 24, 48, and 120 h, was assessed by the dual culture technique. In second place, it was evaluated the application of these fungi separately or in combination during the ex vitro adaptation phase of the vitroplants. In total, 12 treatments were included, the treaments with each fungus and the control without fungus. The vitroplants were transplanted into polypropylene trays of 70 alveolus containing red ferralitic lixiviated soil and organic amendment (1:3 v/v) as substrate. P. chlamydosporia strain IMI SD 187 (KlamiC�� Bionematicide) was applied with T. asperellum strain Ta.13 (SevetriC Biofungicide) by submerging the root before transplanting or by spraying the plant at 3 and 20 days post planting. The trays were randomly arranged in the adaptation area. At 30 days, the plant vegetative growth and the substrate and root colonization by P. chlamydosporia were assessed. In vitro, T. asperellum grew faster than P. chlamydosporia, but there was no direct hyphal contact between them. P. chlamydosporia colonized the root system and the rhizosphere of banana vitroplants when this fungus was applied separately or in combination with T. asperellum; both fungi promoted vitroplant growth. The results show that P. chlamydosporia and T. asperellum are good candidates for banana biomanagement strategies., biomanagement, biological control, endophytic fungi, plant growth promotion

The objective of this study was to validate the application of P. chlamydosporia strain IMI SD 187 (a.i. of bionematicide KlamiC®) in the agronomic management strategy for plantain vitroplants (Musa paradisiaca L.) during the ex vitro adaptation phase. In a first assay, the in vitro compatibility of P. chlamydosporia, with the products FitoMas, Urea, Cuproflow, Bayfolan, and Dimethoate added to the culture medium, was evaluated, taking into account the fungal radial mycelial growth and sporulation. In a second assay, these products were combined with the strain IMI SD 187, during the acclimatization phase of vitroplants. P. chlamydosporia was applied at 5,6 x 105 clamidospore.vitroplant-1 by sprinkling the vitroplants at 3 and 20 days after transplanting, alone or in combination with agrochemicals and bioestimulant, on vitroplants of different size, cultivar ‘CEMSA – ¾’. The vitroplants were transplanted into polypropylene trays of 70 alveolus containing substrate: ferralitic red lixiviade soil and organic amendment (1:3 v/v); and they were distributed in the acclimatization area under randomize block design. The plant growth and fungal colonization on the substrate and roots were evaluated. Cuproflow was considered high toxic and the rest of the products evaluated were compatibles with the fungus. P. chlamydosporia colonized the vitroplants rhizosphere, and plant growth parameters were increased: plant length, pseudo- stem diameter and foliate and root fresh masses. The major growth stimulation was achieved on smaller size vitroplants.