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This document is a report from the journal Antipode, written on the eve of Peer Review Week. The report discusses the importance of technology and innovation in the peer review process, acknowledging that while these systems can be frustrating, they are necessary in a world with increasing numbers of submissions and publications. The report emphasizes the belief that strong peer reviewing is crucial for ensuring the quality and relevance of articles in the journal. The report also expresses gratitude to the almost 600 dedicated reviewers who have contributed to the journal between July 2023 and June 2024. [Extracted from the article]

The document titled "Author index" provides a list of authors and their corresponding articles published in the journal "Ultrasound in Obstetrics & Gynecology." The index includes a wide range of authors and topics, covering various aspects of obstetrics and gynecology. This resource can be useful for library patrons conducting research in these fields, as it provides a comprehensive list of authors and their contributions to the journal. [Extracted from the article]

Kale (Brassica oleracea var. Achepala) is a leaf vegetable plants from the Brassica family that has high potential to develop. The use of nutrients in hydroponic cultivation has important role to fulfil the nutrient needs of curly kale plants. The presence of nitrogen is important for kale in growth and crop result. Cultivation with floating raft system needs to focus into the ideal population to get an optimal growth rate. This study aimed to obtain the correct amount of nitrogen concentration and plant population on the growth and yield of curly kale with floating raft hydroponic system. This research conducted in the Green house experimental field of Agriculture Faculty, University of Brawijaya on August 2022 to November 2022. The research using Factorial Randomized Block Design with 2 factors and 3 replication. The first factor is nitrogen concentration of hydroponic nutrients, and the second factor is plant population. The result implies that Nitrogen concentration 2M with 4 plants population given higher EC value. 1,5M nitrogen concentration on 4 population plants and 2M nitrogen concentration on 6 plants population given the higher leaf area. Combination of nitrogen concentration of 1M and 2M and 8 plant population gives higher fresh total weight per container. 2 M nitrogen concentration given higher number of leaves and EC value in the media, but given shorter result the root length of curly kale than nitrogen concentration of 0,5M. 8 plants population gives lower number of leaves and root length than 4 curly kale plants population. [ABSTRACT FROM AUTHOR]

Microgreens are vegetable greens that are harvested early while they are still immature and have just developed cotyledons. One of the disadvantages and a challenge in production is that they exhibit a short shelf life and may be damaged easily. In seeking to prolong the shelf life, some pre- and postharvest interventions have been investigated. Here, kale and mustard microgreens were grown in a controlled-environment walk-in chamber at +21/17 °C, with ~65% relative air humidity, while maintaining the spectral composition of deep red 61%, blue 20%, white 15%, and far red 4% (150, 200, and 250 µmol m−2 s−1 photosynthetic photon flux density (PPFD)). Both microgreens seemed to exhibit specific and species-dependent responses. Higher PPFD during growth and storage in light conditions resulted in increased contents of TPC in both microgreens on D5. Additionally, 150 and 250 PPFD irradiation affected the α-tocopherol content by increasing it during postharvest storage in kale. On D0 150 for kale and 200 PPFD for mustard microgreens, β-carotene content increased. D5 for kale showed insignificant differences, while mustard responded with the highest β-carotene content, under 150 PPFD. Our findings suggest that both microgreens show beneficial outcomes when stored in light compared to dark and that mild photostress is a promising tool for nutritional value improvement and shelf-life prolongation. [ABSTRACT FROM AUTHOR]

Glucosinolates are compounds found in edible parts of Brassica crops and has been investigated comprenhensivley in last decades as have beneficial effect on human nutrition. Due to a lack of information, fifteen Croatian leafy Brassica landraces were evaluated for leaf glucosinolate content and morphological traits, and to find possible relation between them after field cultivation. Regarding morphological traits we found that most of the studied landraces belong to the collard morphotype with low curling density and leaf blistering and landraces showed high variability in leaf color. Regarding glucosinolates, we found a significant difference in these phytonutrients among landraces. Total glucosinolate content differed 10-fold between landraces with a mean value of 46,9 μmol/g leaf dry weight. Thirteen glucosinolates belonging to the three principal chemical groups were found, including seven aliphatic, four indolic and two aromatic glucosinolates. Seven compounds were found in all landraces. Correlation and multivariate analysis between morphological properties and glucosinolates content gave us limited conclusions, but obtained results provide a profile of kale and collard landraces phytonutrients useful for future research on nutritional value or breeding.

Kale is becoming an important vegetable worldwide, mainly due to its nutritional properties. Kale leaves can be marketed whole, although minimal processing is also in demand. In this article, it was analyzed the effect of packaging in a modified atmosphere of fresh-cut kale leaves stored at 20 °C and 4 °C. Kale leaves were cut into 4 × 4 cm strips and stored in low-density polyethylene bags. Samples processed in the same way but stored in PVC were used as controls. Leaves kept in a modified atmosphere showed a delay in color change with Hue values from about 130 to 120 under PMA against 130 to 100 in control group (CTR) leaves. Chlorophyll degradation was also delayed in both storage temperatures. Samples stored under PMA showed about two times the levels of total chlorophylls with respect to CTR samples at the end of the storage. No changes in total sugar content were detected during storage and no differences were detected between control and modified atmospheres stored samples. Samples maintained in a modified atmosphere showed a lower decrement in soluble proteins and a lower rate of RUBISCO degradation at both temperatures. The relation of RUBISCO content PMA/CTR ranged from 1 to about 3 toward the end of storage No changes in phenols content were found when comparing control and treated samples. However, flavonoid and the antioxidant contents increased in samples stored in modified atmospheres with respect to their controls. We demonstrated that storage in modified atmospheres could be an adequate and simple methodology to extend postharvest life of this minimally processed product at both ambient and refrigerated storage. [ABSTRACT FROM AUTHOR]

Abstract Background Kale, a versatile cruciferous crop, valued for its pro-health benefits, stress resistance, and potential applications in forage and cosmetics, holds promise for further enhancement of its bioactive compounds through in vitro cultivation methods. Micropropagation techniques use cytokinins (CKs) which are characterized by various proliferative efficiency. Despite the extensive knowledge regarding CKs, there remains a gap in understanding their role in the physiological mechanisms. That is why, here we investigated the effects of three CKs – kinetin (Kin), 6-benzylaminopurine (BAP), and 2-isopentenyladenine (2iP) – on kale physiology, antioxidant status, steroidal metabolism, and membrane integrity under in vitro cultivation. Results Our study revealed that while BAP and 2iP stimulated shoot proliferation, they concurrently diminished pigment levels and photosynthetic efficiency. Heightened metabolic activity in response to all CKs was reflected by increased respiratory rate. Despite the differential burst of ROS, the antioxidant properties of kale were associated with the upregulation of guaiacol peroxidase and the scavenging properties of ascorbate rather than glutathione. Notably, CKs fostered the synthesis of sterols, particularly sitosterol, pivotal for cell proliferation and structure of membranes which are strongly disrupted under the action of BAP and 2iP possibly via pathway related to phospholipase D and lipoxygenase which were upregulated. Intriguingly, both CKs treatment spurred the accumulation of sitostenone, known for its ROS scavenging and therapeutic potential. The differential effects of CKs on brassicasterol levels and brassinosteroid (BRs) receptor suggest potential interactions between CKs and BRs. Conclusion Based on the presented results we conclude that the effect evoked by BAP and 2iP in vitro can improve the industrial significance of kale because this treatment makes possible to control proliferation and/or biosynthesis routes of valuable beneficial compounds. Our work offers significant insights into the nuanced effects of CKs on kale physiology and metabolism, illuminating potential avenues for their application in plant biotechnology and medicinal research.

Relevance. Cabbage Kale (Brassica oleracea L. var. acephala) is a green, red and purple leafy vegetable cultivated in Northern and Central Europe, as well as North America. Kale deservedly occupies a high place in the list of functional products due to its high content of biologically active substances. Kale contains vitamins, minerals, antioxidant compounds, carbohydrates, unsaturated fatty acids, proteins and dietary fiber. Despite its high attractiveness as a functional food product, it is not cultivated on a massive scale in Russia.Methodology. The purpose of this work is to study the content of phenolic compounds in two varieties of kale cabbage to substantiate recommendations for its cultivation in Russia. The variety and hybrid of kale cabbage – Dwarf Blue Scotch with green leaves and the hybrid Redbor F1 with red leaves – were used as the object of the study. The content of soluble phenolic compounds and flavonoids, as well as the qualitative composition of phenolic compounds in kale cabbage, was studied. Determination was carried out according to generally accepted methods. Grown plants at the age of 16 weeks were used as samples for analysis. The experiment was carried out in laboratory conditions in 5 replicates.Results. The results of the studies indicate a fairly high content of phenolic compounds and flavonoids in the studied varieties of kale cabbage. It has been demonstrated that the Redbor F1 hybrid is characterized by a higher content of soluble phenolic compounds compared to the Dwarf Blue Scotch variety by an average of ~20%. It was concluded that this Redbor F1 hybrid can be used to develop methods for increasing the efficiency of the synthesis of secondary metabolites that are beneficial to human health, and is recommended to farmers for cultivation in the Russian Federation.

Incorporating Internet of Things (IoT) technology into indoor kale cultivation holds significant promise for revolutionizing organic farming methodologies. While numerous studies have investigated the impact of environmental factors on kale growth in IoT-based smart agricultural systems, such as temperature, humidity, and nutrient levels, indoor ultraviolet (UV) LED light’s operational efficiencies and advantages in organic farming still need to be explored. This study assessed the efficacy of 15 UV light-controlling indoor experiments in three distinct lighting groups: kale cultivated using conventional household LED lights, kale cultivated using specialized indoor UV lights designed for plant cultivation, and kale cultivated using hybrid household and LED grow lights. The real-time IoT-based monitoring of light, soil, humidity, and air conditions, as well as automated irrigation using a water droplet system, was employed throughout the experiment. The experimental setup for air conditioning maintained temperatures at a constant 26 degrees Celsius over the 45-day study period. The results revealed that a combination of daylight household lights and indoor 4000 K grow lights scored the highest, indicating optimal growth conditions. The second group exposed to warm white household and indoor grow red light exhibited slightly lower scores but larger leaf size than the third group grown under indoor grow red light, likely attributable to reduced light intensity or suboptimal nutrient levels. This study highlights the potential of indoor UV LED light farming to address challenges posed by urbanization and climate change, thereby contributing to efforts to mitigate agricultural carbon emissions and enhance food security in urban environments. This research contributes to positioning kale as a sustainable organic superfood by optimizing kale cultivation.

Microgreens and baby leaves are increasingly appreciated and consumed due to the increased concentrations of antioxidant compounds, vitamins, nutrients, and minerals than adult plants. The objective was to evaluate the effect of different substrates and protected environments on the growth of microgreen and baby leaf kale (Brassica oleracea var. sabellica L.) and their contents of carotenoids and chlorophylls. The experiments were carried out in a completely randomized design in a factorial arrangement of 4 × 2 (four environments × two substrates) for the microgreens and 4 × 5 (four environments × five substrates) for the baby leaf, both with six replications. For microgreens, the Carolina Soil® commercial substrate promoted greater height, fresh matter, dry matter, productivity, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in all evaluated environments. In the greenhouse with a screen of 35% shading under the film, greater height, fresh matter, dry matter, and productivity were obtained, and in the greenhouse with a screen of 42/50% shading under the film, higher contents of microgreen chlorophylls and carotenoids were obtained. For baby leaves, the greenhouse with 42/50% shading screen under the film promoted plants with greater height, fresh matter, dry matter, and productivity in all substrates. The baby leaf grown in the greenhouse with a screen of 35% shading under the film showed higher chlorophyll and carotenoid contents in the pure Carolina Soil® substrate. For greater productivity, with higher contents of pigments, greenhouses with screens under the film and the use of organomineral commercial substrate are the most suitable for microgreen and baby leaf kale. [ABSTRACT FROM AUTHOR]

SUMMARY: Anthocyanin is an important pigment responsible for plant coloration and beneficial to human health. Kale (Brassica oleracea var. acephala), a primary cool‐season flowers and vegetables, is an ideal material to study anthocyanin biosynthesis and regulation mechanisms due to its anthocyanin‐rich leaves. However, the underlying molecular mechanism of anthocyanin accumulation in kale remains poorly understood. Previously, we demonstrated that BoDFR1 is a key gene controlling anthocyanin biosynthesis in kale. Here, we discovered a 369‐bp InDel variation in the BoDFR1 promoter between the two kale inbred lines with different pink coloration, which resulted in reduced transcriptional activity of the BoDFR1 gene in the light‐pink line. With the 369‐bp insertion as a bait, an R2R3‐MYB repressor BoMYB4b was identified using the yeast one‐hybrid screening. Knockdown of the BoMYB4b gene led to increased BoDFR1 expression and anthocyanin accumulation. An E3 ubiquitin ligase, BoMIEL1, was found to mediate the degradation of BoMYB4b, thereby promoting anthocyanin biosynthesis. Furthermore, the expression level of BoMYB4b was significantly reduced by light signals, which was attributed to the direct repression of the light‐signaling factor BoMYB1R1 on the BoMYB4b promoter. Our study revealed that a novel regulatory module comprising BoMYB1R1, BoMIEL1, BoMYB4b, and BoDFR1 finely regulates anthocyanin accumulation in kale. The findings aim to establish a scientific foundation for genetic improvement of leaf color traits in kale, meanwhile, providing a reference for plant coloration studies. [ABSTRACT FROM AUTHOR]

The properties of kale as a functional food are well established. We sought to determine how fermentation further enhances these properties. We tested different fermentation conditions: (i) spontaneous fermentation with naturally occurring bacteria, (ii) spontaneous fermentation with 2% salt, (iii) Lactococcus lactis, (iv) Lactobacillus acidophilus, (v) mixture of L. lactis and L. acidophilus, (vi) mixture of L. lactis, L. acidophilus, and Clostridium butyricum. We quantified selected bioactive components using high‐performance liquid chromatography (HPLC) and antinutritional factors using a gravimetric method and spectrophotometry. We then determined (i) the antioxidant capacity of the vegetable, (ii) anti‐inflammation capacity, and (iii) the surface microbiota composition by 16S sequencing. All fermentation methods imparted some benefits. However, fermentation with mixed culture of L. lactis and L. acidophilus was most effective in increasing polyphenols and sulforaphane accessibility, increasing antioxidant activity, and reducing antinutritional factors. Specifically, fermentation with L. lactis and L. acidophilus increased total polyphenols from 8.5 to 10.7 mgGAE/g (milligrams of gallium acid equivalent per gram) and sulforaphane from 960.8 to 1777 μg/g (microgram per gram) but decreased the antinutritional factors oxalate and tannin. Total oxalate was reduced by 49%, while tannin was reduced by 55%–65%. The antioxidant capacity was enhanced but not the anti‐inflammation potential. Both unfermented and fermented kale protected equally against lipopolysaccharide (LPS)‐induced inflammation in RAW 264.7 macrophages and prevented increases in inducible nitric oxide synthase (iNOS), tumor necrosis factor‐alpha (TNF‐α), interleukin‐1 beta (IL‐1β), and interleukin‐6 messenger RNA (IL‐6 mRNA) expression by 84.3%, 62%, 68%, and 85.5%, respectively. Unfermented and naturally fermented kale had high proportions of sulfur reducing Desulfubrio and Proteobacteria usually associated with inflammation. Fermenting with L. lactis and/or L. acidophilus changed the bacterial proportions, reducing the Proteobacteria while increasing the genera Lactobacilli and Lactococcus. In summary, fermentation enhances the well‐known beneficial impacts of kale. Fermentation with mixed cultures of L. lactis and L. acidophilus imparts higher benefits compared to the single cultures or fermentation with native bacteria present in the vegetable. [ABSTRACT FROM AUTHOR]

Hyperkalemia is common among patients with end stage kidney disease. Management involves diet modification. Hot water blanching is recommended to leach potassium in vegetables which results in losses of water‐soluble and heat labile vitamins. Evidence on the effect of blanching in reducing potassium level of locally consumed vegetables in Kenya is limited. This study sought to establish effect of hot water blanching time‐temperature on level of potassium, vitamin B1, B3 and C in kales (Brassica oleracea var. acephala) and spinach (Spinach oleracea) on potassium and vitamins B1, B3 and C retention/loss. The study adopted a full factorial experimental design. Vitamins were determined using high performance liquid chromatography. Potassium was quantified using atomic absorption spectrophotometry. To compare nutrient content between samples, independent t‐test and Analysis of Variance were used at 95% confidence level. Nutrient content of fresh kales and spinach were potassium (102 mg/100 g and 615 mg/100 g), vitamin B1 (124 μg/100 g and 51 μg/100 g), vitamin B3 (1165 μg/100 g and 812 μg/100 g) and vitamin C (102 mg/100 g and 116 mg/100 g) respectively. In kales, blanching for 20 min at 1000°C resulted to retention of 86.9%, 55.6%, 27.6% and 12.9% of vitamin B1, B3, C and potassium respectively. In spinach, blanching for 20 min at 1000°C resulted in retention of 79.9%, 88.6%, 12.2% and 40.6% retention of vitamin B1, B3, C and potassium respectively. Vitamin C and Potassium were the most sensitive to heat and leaching. Time had a greater effect than temperature in this study. This study recommends blanching of kale at 15.2 min at 800°C, spinach at 17.7 min at 840°C. Further research on optimal blanching time‐temperature for potassium and vitamin retention/loss is recommended. [ABSTRACT FROM AUTHOR]

Background: Kale, a versatile cruciferous crop, valued for its pro-health benefits, stress resistance, and potential applications in forage and cosmetics, holds promise for further enhancement of its bioactive compounds through in vitro cultivation methods. Micropropagation techniques use cytokinins (CKs) which are characterized by various proliferative efficiency. Despite the extensive knowledge regarding CKs, there remains a gap in understanding their role in the physiological mechanisms. That is why, here we investigated the effects of three CKs – kinetin (Kin), 6-benzylaminopurine (BAP), and 2-isopentenyladenine (2iP) – on kale physiology, antioxidant status, steroidal metabolism, and membrane integrity under in vitro cultivation. Results: Our study revealed that while BAP and 2iP stimulated shoot proliferation, they concurrently diminished pigment levels and photosynthetic efficiency. Heightened metabolic activity in response to all CKs was reflected by increased respiratory rate. Despite the differential burst of ROS, the antioxidant properties of kale were associated with the upregulation of guaiacol peroxidase and the scavenging properties of ascorbate rather than glutathione. Notably, CKs fostered the synthesis of sterols, particularly sitosterol, pivotal for cell proliferation and structure of membranes which are strongly disrupted under the action of BAP and 2iP possibly via pathway related to phospholipase D and lipoxygenase which were upregulated. Intriguingly, both CKs treatment spurred the accumulation of sitostenone, known for its ROS scavenging and therapeutic potential. The differential effects of CKs on brassicasterol levels and brassinosteroid (BRs) receptor suggest potential interactions between CKs and BRs. Conclusion: Based on the presented results we conclude that the effect evoked by BAP and 2iP in vitro can improve the industrial significance of kale because this treatment makes possible to control proliferation and/or biosynthesis routes of valuable beneficial compounds. Our work offers significant insights into the nuanced effects of CKs on kale physiology and metabolism, illuminating potential avenues for their application in plant biotechnology and medicinal research. [ABSTRACT FROM AUTHOR]

This study, which aims to draw attention to the historical features of Maraş Castle and the importance of preserving it, examines the changes Maraş Castle has undergone, through visual documents. Maraş Castle, located in the central district of the Kahramanmaraş Province, is a cultural heritage that has witnessed the five-thousand-year historical course of the city. The first settlements in the castle can be traced back to the beginning of the early Bronze Age. In addition to being surrounded by Roman era fortifications and harbouring the Late Hittite period archaeological remains, Maraş Castle, referred to as the traditional city centre since the Dulkadiroğlu era, has had an important place in the urban memory. In the data collection process, first, old photographs and engravings of the castle were obtained. Then, the literature review results related to the castle's architecture and history were evaluated together with field observations. It was found out that Maraş Caste has served different functions and undergone numerous changes in its history. [ABSTRACT FROM AUTHOR]

Cruciferous sprout is a new form of vegetable product rich in bioactive compounds, especially glucosinolates. Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators, with a particular focus on their contribution to nutritional quality and health benefits. Nevertheless, the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear. In this study, it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates. The 5 μmol.L-1 melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts. Notably, the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation. Furthermore, our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51, which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively, were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation. Additionally, the expression of glucosinolate biosynthetic genes, including BoaCYP79F1, BoaCYP83A1, BoaSUR1, BoaUGT74B1, BoaCYP79B2, BoaCYP79B3, and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5, BoaAOP2, BoaCYP81F2, and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate, was regulated by melatonin or UV-B irradiation. Taken together, these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts. [ABSTRACT FROM AUTHOR]

UV-A (315–400 nm) is a component of solar radiation, which impacts plant physiology. Consequently, the impact was investigated of UV-A supplementation on various aspects of kale cultivation in a plant factory with artificial lighting. Three UV-A intensity treatments (5, 10, or 15 W/m2) were applied to kale plants for 5 days before harvesting. The results revealed that 5 or 10 W/m2 UV-A supplementation provided significant advantages for kale growth. The fresh weight of the kale shoots increased by over 35–50% after UV-A exposure. In particular, the 10 W/m2 UV-A exposure significantly improved the photosynthetic rate, water-use efficiency, and quantum efficiency of photosystem II (Y(II)). In addition, the treatment with UV-A positively impacted changes in leaf spectral indices, resulting in decreases in the simple ratio index (SR), the normalized phaeophytinization index (NPQI), and the normalized difference red edge index (NDRE). Furthermore, the phytonutrients in kale (Vitamin C, soluble protein, and total phenolics) were enhanced using the 10 W/m2 UV-A treatment. One notable outcome was the consistent reduction in nitrate contents across all UV-A treatment intensities. Therefore, supplementing kale cultivation with UV-A radiation was a valuable strategy for enhancing kale yield and quality in plant factory production. [ABSTRACT FROM AUTHOR]

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Supramolecular solvent-based extraction (SUPRAS) stands out as a promising approach, particularly due to its environmentally friendly and efficient characteristics. This research explores the optimization of SUPRAS extraction for sango radish and kale microgreens, focusing on enhancing the extraction efficiency. The Taguchi experimental design and artificial neural network (ANN) modeling were utilized to systematically optimize extraction parameters (ethanol content, SUPRAS: equilibrium ratio, centrifugation rate, centrifugation time, and solid-liquid ratio). The extraction efficiency was evaluated by measuring the antioxidant activity (DPPH assay) and contents of chlorophylls, carotenoids, phenolics, and anthocyanidins. The obtained results demonstrated variability in phytochemical contents and antioxidant activities across microgreen samples, with the possibility of achieving high extraction yields using the prediction of optimized parameters. The optimal result for sango radish can be achieved at an ethanol content of 35.7%; SUPRAS: equilibrium ratio of 1 v/v, centrifugation rate of 4020 rpm, centrifugation time of 19.84 min, and solid-liquid ratio of 30.2 mg/mL. The following parameters are predicted for maximal extraction efficiency for kale: ethanol content of 35.64%; SUPRAS: equilibrium ratio of 1 v/v; centrifugation rate of 3927 rpm; centrifugation time of 19.83 min; and solid-liquid ratio of 30.4 mg/mL. Additionally, laboratory verification of predicted SUPRAS parameters showed very low divergency degrees for both microgreens (–3.09 to 2.36% for sango radish, and −2.57 to 3.58% for kale). This potential of SUPRAS extraction, coupled with statistical and computational optimization techniques, can enhance the recovery of valuable bioactive compounds from microgreens and contribute to green extraction applications. [ABSTRACT FROM AUTHOR]

Iodine deficiency in the diet globally continues to be a cause of many diseases and disabilities. Kale is a vegetable that has health-promoting potential because of many nutrients and bioactive compounds (ascorbic acid, carotenoids, glucosinolates and phenolic compounds). Brassica vegetables, including kale, have been strongly recommended as dietary adjuvants for improving health. The nutrient and health-promoting compounds in kale are significantly affected by thermal treatments. Changes in phytochemicals upon such activities may result from two contrary phenomena: breakdown of nutrients and bioactive compounds and a matrix softening effect, which increases the extractability of phytochemicals, which may be especially significant in the case of iodine-fortified kale. This study investigated changes of basic composition, iodine, vitamin C, total carotenoids and polyphenols contents as well as antioxidant activity caused by steaming, blanching and boiling processes in the levels of two cultivars of kale (green and red) non-biofortified and biofortified via the application to nutrient solutions in hydroponic of two iodoquinolines [8-hydroxy-7-iodo-5-quinolinesulfonic acid (8-OH-7-I-5QSA) and 5-chloro-7-iodo-8-quinoline (5-Cl-7-I-8-Q)] and KIO3. Thermal processes generally significantly reduced the content of the components in question and the antioxidant activity of kale, regardless of cultivar and enrichment. It was observed that the red cultivar of kale had a greater ability to accumulate and reduce iodine losses during the culinary processes. 8-hydroxy-7-iodo-5-quinolinesulfonic acid showed a protective effect against the treatments used, compared to other enrichments, thus contributing to the preservation of high iodine content. [ABSTRACT FROM AUTHOR]

Unpleasant flavor is a commonly stated reason for avoiding vegetables. However, repeated exposure to flavors, even unpleasant vegetable flavors, improves acceptability. Yet, increasing exposure to vegetables is difficult, as vegetables can be less convenient, available, and affordable than other foods. This study describes a method to circumvent these barriers to repeated flavor exposure. We designed a game with vegetable‐ or control‐flavored gummies, where players (N = 34) were challenged to identify the flavors over 2 weeks. One round was played per day, and the questions and gummies were designed to make it harder to identify the flavors as the game progressed. At screening, qualified subjects needed to consume <2.5 servings of nonstarchy vegetables per day as well as dislike at least one “target” and one “non‐target” vegetable. The “target” vegetables (kale and broccoli) were included in the game and the “non‐target” vegetables (asparagus and spinach) were included in sensory tests but not the game. Vegetable liking ratings were measured at baseline (before gameplay), after 1 week of gameplay, and after 2 weeks of gameplay. Pilot data indicate one target vegetable (kale) increased in liking after 1 and 2 weeks of gameplay among the vegetable group, but not the control group. Liking for broccoli (other target vegetable), as well as asparagus and spinach (nontarget vegetables), did not significantly change for either group. Thus, our “gamified” approach to vegetable flavor exposure may be useful in increasing acceptance of some vegetables, but additional work to identify why the game improved liking for kale but not broccoli is needed. [ABSTRACT FROM AUTHOR]

Summary: The study investigated the effect of different spices–cardamom, chilli, ginger, and turmeric on the bioactive compounds, antioxidant activity, colour, and sensory attributes of kale crisps. Specifically, the antioxidant capacity was ranked as follows: kale crisps with turmeric > chilli ≈ ginger > cardamom ≈ control crisps. The analysis using HPLC‐MS/MS focused on 15 specific compounds. Kale crisps contained predominantly chlorogenic and isochlorogenic acids, with turmeric crisps having the highest levels. Turmeric kale crisps exhibited the best sensory properties for overall appearance and crispness. The study found that kale crisps have a high folate content, with ginger crisps containing the highest amounts. Cardamom kale crisps preserved high folate content but had a strong aroma and bitter flavour. The study found lightness (L*) positively correlated with aroma desirability, while redness (a*) negatively correlated with flavour desirability. The chilli kale crisps had a strong flavour and lost their green colour the most, resulting in a lower panel ranking despite high antioxidant potential. This study indicates that kale crisps, especially those with turmeric, may represent a valuable source of bioactive compounds and functional properties. Sensory evaluation indicated their high levels of acceptability. [ABSTRACT FROM AUTHOR]

The increasing demand for soilless media, sustainability issues with peatmoss, and increasing cost of peatmoss have prompted studies of more environmentally friendly and less expensive substitutes. Biochar, a lightweight black carbon material produced by the pyrolysis of biomass, has gained popularity as a soilless media supplement. The objective of this study was to evaluate Eastern red cedar (ERC) biochar as a supplement to soilless media for the production of chrysanthemum and ornamental kale. Treatments included ERC biochar produced at three different temperature ranges of 300 to 350 °C, 400 to 450 °C, and 500 to 550 °C that were applied at 25%, 50%, and 75% v/v plus a control (100% v/v of standard commercial mix). Additionally, ERC bark was applied at the same rate as biochar. The 300 to 350 °C and 400 to 450°C temperature ranges increased the bulk density of the media, whereas total porosity was greatest with just bark. Regarding the physical properties of the media, in general, the 75% v/v supplementation rate of ERC bark or biochar at any temperature increased air porosity but decreased the water holding capacity, except for the water holding capacity at 500 to 550 °C. As the biochar production temperature increased, so did the pH and electrical conductivity (EC), whereas volatile matter decreased. Plant height, width, shoot dry weight, root dry weight, number of flowers (chrysanthemum only), flower diameter, and water use efficiency were greatest with the 100% v/v soilless media for both species. In general, chrysanthemum plants grown with 25% v/v biochar supplementation or bark had similar height, width, and shoot dry weight at any temperature compared with those grown with the 100% v/v soilless media. For ornamental kale, the 25% v/v 400 to 450 °C biochar supplementation showed plant height and water use efficiency similar to those of the 100% v/v soilless media. In general, 25% ERC bark performed similar to 25% v/v and 50% v/v biochar at any temperature for plant width, shoot dry weight, root dry weight, water use efficiency, and root-to-shoot ratio. The media nutrient content and EC were greater with 100% v/v soilless media and a lower rate (25% v/v) of ERC bark and biochar than with higher rates. The higher levels of biochar were harmful and reduced the ornamental kale growth and quality. These results suggest that supplemented soilless media with lower rates (25% v/v) of ERC biochar could be recommended for chrysanthemum, but that less than 25% v/v may be necessary for ornamental kale. [ABSTRACT FROM AUTHOR]

Seventy-eight Rhizoctonia isolates were obtained from brassicas in five Brazilian states between 2015 and 2017. The isolates were identified by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS) and tested for pathogenicity and aggressiveness. Sixty-eight isolates were identified as R. solani and 10 isolates as binucleate Rhizoctonia. The most prevalent anastomosis group (AG) was AG-4 HG-I (83.3% of isolates), followed by AG-A (7.7%), AG-1 IB (2.6%), AG-G (2.6%), AG-R (2.6%) and AG-2-2 IIIB (1.3%). AG-4 HG-I isolates were widely distributed and recovered from all Brazilian states sampled, including 12 brassicas-growing fields. All isolates were pathogenic to broccoli, cauliflower and kale, causing necrotic lesions on subterraneous stems. There was no significant difference in aggressiveness among the different AGs in broccoli, but AG-1 IB isolates were more aggressive to cauliflower and AG-4 HG-I isolates were more aggressive to kale. This study is the first report of AG-4 HG-I, AG-2-2 IIIB, AG-A, AG-G and AG-R infecting brassica plants in Brazil. To our knowledge, this is the first detailed research of the AG composition of Rhizoctonia populations causing brassica diseases in Brazil. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the effect of two distances: close (0–10 m) and far (60 m) from the heavy traffic roadside, at three different cultivation sites (MS: Mevlanakapi–Silivrikapi, SB: Silivrikapi–Belgradkapi, and BY: Belgradkapi–Yedikule kapi) along the road line. First, the phenolic compounds, antioxidant activity, and physicochemical properties in kale and arugula vegetables were examined. Second, heavy metal concentrations in vegetables, soil, and irrigated water were investigated. In both vegetables, the highest total phenolic content was detected in samples obtained from far distance in SB site (3880.3 mg/kg) for kale and in BY site (1459.9 mg/kg) for arugula, whereas the lowest content was found at the close distance in MS site for both kale (448.5 mg/kg) and arugula (586.4 mg/kg). The antioxidant activity values [mg Trolox/kg (dw)] ranged from 366.74 to 586.10 and 2349.00 to 3757.4 for kale and from 520.00 to 945.60 and 3323.00 to 5814.70 for arugula in 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) and 2,2‐diphenyl‐1‐picrylhydrazyl methods, respectively. The levels of Cd and Hg in kale and arugula and Fe content in arugula exceeded FAO/WHO permissible limits, making them unsafe for human consumption. Meanwhile, the Pb content in kale and arugula and Fe content in kale were observed to be within acceptable limits set by FAO/WHO. In the irrigated water, the Pb value was below the permissible limit, whereas the Cd value was above it and no Hg and Fe were detected. In the soil samples, the Pb and Fe values were below the limit, whereas the Cd and Hg values were higher. [ABSTRACT FROM AUTHOR]

Incorporating Internet of Things (IoT) technology into indoor kale cultivation holds significant promise for revolutionizing organic farming methodologies. While numerous studies have investigated the impact of environmental factors on kale growth in IoT-based smart agricultural systems, such as temperature, humidity, and nutrient levels, indoor ultraviolet (UV) LED light's operational efficiencies and advantages in organic farming still need to be explored. This study assessed the efficacy of 15 UV light-controlling indoor experiments in three distinct lighting groups: kale cultivated using conventional household LED lights, kale cultivated using specialized indoor UV lights designed for plant cultivation, and kale cultivated using hybrid household and LED grow lights. The real-time IoT-based monitoring of light, soil, humidity, and air conditions, as well as automated irrigation using a water droplet system, was employed throughout the experiment. The experimental setup for air conditioning maintained temperatures at a constant 26 degrees Celsius over the 45-day study period. The results revealed that a combination of daylight household lights and indoor 4000 K grow lights scored the highest, indicating optimal growth conditions. The second group exposed to warm white household and indoor grow red light exhibited slightly lower scores but larger leaf size than the third group grown under indoor grow red light, likely attributable to reduced light intensity or suboptimal nutrient levels. This study highlights the potential of indoor UV LED light farming to address challenges posed by urbanization and climate change, thereby contributing to efforts to mitigate agricultural carbon emissions and enhance food security in urban environments. This research contributes to positioning kale as a sustainable organic superfood by optimizing kale cultivation. [ABSTRACT FROM AUTHOR]

ObjectiveKale is an important bulk vegetable crop worldwide, its main growth characteristics are outer leaves and leaf bulbs. The traits of leaf bulb kale are crucial for adjusting water and fertilizer parameters in the field to achieve maximum yield. However, various factors such as soil quality, light exposure, leaf overlap, and shading can affect the growth of in practical field conditions. The similarity in color and texture between leaf bulbs and outer leaves complicates the segmentation process for existing recognition models. In this paper, the segmentation of kale outer leaves and leaf bulbs in complex field background was proposed, using pixel values to determine leaf bulb size for intelligent field management. A semantic segmentation algorithm, UperNet-ESA was proposed to efficiently and accurately segment nodular kale outer leaf and leaf bulb in field scenes using the morphological features of the leaf bulbs and outer leaves of nodular kale to realize the intelligent management of nodular kale in the field.MethodsThe UperNet-ESA semantic segmentation algorithm, which uses the unified perceptual parsing network (UperNet) as an efficient semantic segmentation framework, is more suitable for extracting crop features in complex environments by integrating semantic information across different scales. The backbone network was improved using ConvNeXt, which is responsible for feature extraction in the model. The similarity between kale leaf bulbs and outer leaves, along with issues of leaf overlap affecting accurate target contour localization, posed challenges for the baseline network, leading to low accuracy. ConvNeXt effectively combines the strengths of convolutional neural networks (CNN) and Transformers, using design principles from Swin Transformer and building upon ResNet50 to create a highly effective network structure. The simplicity of the ConvNeXt design not only enhances segmentation accuracy with minimal model complexity, but also positions it as a top performer among CNN architectures. In this study, the ConvNeXt-B version was chosen based on considerations of computational complexity and the background characteristics of the knotweed kale image dataset. To enhance the model's perceptual acuity, block ratios for each stage were set at 3:3:27:3, with corresponding channel numbers of 128, 256, 512 and 1 024, respectively. Given the visual similarity between kale leaf bulbs and outer leaves, a high-efficiency channel attention mechanism was integrated into the backbone network to improve feature extraction in the leaf bulb region. By incorporating attention weights into feature mapping through residual inversion, attention parameters were cyclically trained within each block, resulting in feature maps with attentional weights. This iterative process facilitated the repeated training of attentional parameters and enhanced the capture of global feature information. To address challenges arising from direct pixel addition between up-sampling and local features, potentially leading to misaligned context in feature maps and erroneous classifications at kale leaf boundaries, a feature alignment module and feature selection module were introduced into the feature pyramid network to refine target boundary information extraction and enhance model segmentation accuracy.Results and DiscussionsThe UperNet-ESA semantic segmentation model outperforms the current mainstream UNet model, PSPNet model, DeepLabV3+ model in terms of segmentation accuracy, where mIoU and mPA reached 92.45% and 94.32%, respectively, and the inference speed of up to 16.6 frames per second (fps). The mPA values were better than that of the UNet model, PSPNet model, ResNet-50 based, MobilenetV2, and DeepLabV3+ model with Xception as the backbone, showing improvements of 11.52%, 13.56%, 8.68%, 4.31%, and 6.21%, respectively. Similarly, the mIoU exhibited improvements of 12.21%, 13.04%, 10.65%, 3.26% and 7.11% compared to the mIoU of the UNet-based model, PSPNet model, and DeepLabV3+ model based on the ResNet-50, MobilenetV2, and Xception backbones, respectively. This performance enhancement can be attributed to the introduction of the ECA module and the improvement made to the feature pyramid network in this model, which strengthen the judgement of the target features at each stage to obtain effective global contextual information. In addition, although the PSPNet model had the fastest inference speed, the overall accuracy was too low to for developing kale semantic segmentation models. On the contrary, the proposed model exhibited superior inference speed compared to all other network models.ConclusionsThe experimental results showed that the UperNet-ESA semantic segmentation model proposed in this study outperforms the original network in terms of performance. The improved model achieves the best accuracy-speed balance compared to the current mainstream semantic segmentation networks. In the upcoming research, the current model will be further optimized and enhanced, while the kale dataset will be expanded to include a wider range of samples of nodulated kale leaf bulbs. This expansion is intended to provide a more robust and comprehensive theoretical foundation for intelligent kale field management.

Malathion® is a persistent organophosphate pesticide used against biting and chewing insects on vegetables. It is a difficult-to-remove surface contaminant of vegetables and contaminates surface and ground water and soils. Malathion® is only partially water soluble, but use of detergent carriers makes adhering Malathion® residues difficult to subsequently remove. Magnetically treated water (MTW) successfully removed Malathion® from Chinese Kale (Brassica oleracea L.), meeting Maximum Residue Load (MRL) standards. Samples were soaked in MTW for 30 min prior to detection with GC/MS/MS, 98.5±3.02% of Malathion® was removed after washing by MTW. Removal by simple washing was only ≈42±1.2% which was not nearly sufficient to meet MRL criteria. [ABSTRACT FROM AUTHOR]

The kale crop is an important bulk vegetable, and automatic segmentation to recognize kale is fundamental for effective field management. However, complex backgrounds and texture-rich edge details make fine segmentation of kale difficult. To this end, we constructed a kale dataset in a real field scenario and proposed an UperNet semantic segmentation model with a Swin transformer as the backbone network and improved the model according to the growth characteristics of kale. Firstly, a channel attention module (CAM) is introduced into the Swin transformer module to improve the representation ability of the network and enhance the extraction of kale outer leaf and leaf bulb information; secondly, the extraction accuracy of kale target edges is improved in the decoding part by designing an attention refinement module (ARM); lastly, the uneven distribution of classes is solved by modifying the optimizer and loss function to solve the class distribution problem. The experimental results show that the improved model in this paper has excellent performance in feature extraction, and the average intersection and merger ratio (mIOU) of the improved kale segmentation can be up to 91.2%, and the average pixel accuracy (mPA) can be up to 95.2%, which is 2.1 percentage points and 4.7 percentage points higher than the original UperNet model, respectively, and it effectively improves the segmentation recognition of kale. [ABSTRACT FROM AUTHOR]

[Objective] Kale is an important bulk vegetable crop worldwide, its main growth characteristics are outer leaves and leaf bulbs. The traits of leaf bulb kale are crucial for adjusting water and fertilizer parameters in the field to achieve maximum yield. However, various factors such as soil quality, light exposure, leaf overlap, and shading can affect the growth of in practical field conditions. The similarity in color and texture between leaf bulbs and outer leaves complicates the segmentation process for existing recognition models. In this paper, the segmentation of kale outer leaves and leaf bulbs in complex field background was proposed, using pixel values to determine leaf bulb size for intelligent field management. A semantic segmentation algorithm, UperNet-ESA was proposed to efficiently and accurately segment nodular kale outer leaf and leaf bulb in field scenes using the morphological features of the leaf bulbs and outer leaves of nodular kale to realize the intelligent management of nodular kale in the field. [Methods] The UperNet-ESA semantic segmentation algorithm, which uses the unified perceptual parsing network (UperNet) as an efficient semantic segmentation framework, is more suitable for extracting crop features in complex environments by integrating semantic information across different scales. The backbone network was improved using ConvNeXt, which is responsible for feature extraction in the model. The similarity between kale leaf bulbs and outer leaves, along with issues of leaf overlap affecting accurate target contour localization, posed challenges for the baseline network, leading to low accuracy. ConvNeXt effectively combines the strengths of convolutional neural networks (CNN) and Transformers, using design principles from Swin Transformer and building upon ResNet50 to create a highly effective network structure. The simplicity of the ConvNeXt design not only enhances segmentation accuracy with minimal model complexity, but also positions it as a top performer among CNN architectures. In this study, the ConvNeXtB version was chosen based on considerations of computational complexity and the background characteristics of the knotweed kale image dataset. To enhance the model's perceptual acuity, block ratios for each stage were set at 3:3:27:3, with corresponding channel numbers of 128, 256, 512 and 1 024, respectively. Given the visual similarity between kale leaf bulbs and outer leaves, a high-efficiency channel attention mechanism was integrated into the backbone network to improve feature extraction in the leaf bulb region. By incorporating attention weights into feature mapping through residual inversion, attention parameters were cyclically trained within each block, resulting in feature maps with attentional weights. This iterative process facilitated the repeated training of attentional parameters and enhanced the capture of global feature information. To address challenges arising from direct pixel addition between up-sampling and local features, potentially leading to misaligned context in feature maps and erroneous classifications at kale leaf boundaries, a feature alignment module and feature selection module were introduced into the feature pyramid network to refine target boundary information extraction and enhance model segmentation accuracy. [Results and Discussions] The UperNet-ESA semantic segmentation model outperforms the current mainstream UNet model, PSPNet model, DeepLabV3+ model in terms of segmentation accuracy, where mIoU and mPA reached 92.45% and 94.32%, respectively, and the inference speed of up to 16.6 frames per second (fps). The mPA values were better than that of the UNet model, PSPNet model, ResNet-50 based, MobilenetV2, and DeepLabV3+ model with Xception as the backbone, showing improvements of 11.52%, 13.56%, 8.68%, 4.31%, and 6.21%, respectively. Similarly, the mIoU exhibited improvements of 12.21%, 13.04%, 10.65%, 3.26% and 7.11% compared to the mIoU of the UNet-based model, PSPNet model, and DeepLabV3+ model based on the ResNet-50, MobilenetV2, and Xception backbones, respectively. This performance enhancement can be attributed to the introduction of the ECA module and the improvement made to the feature pyramid network in this model, which strengthen the judgement of the target features at each stage to obtain effective global contextual information. In addition, although the PSPNet model had the fastest inference speed, the overall accuracy was too low to for developing kale semantic segmentation models. On the contrary, the proposed model exhibited superior inference speed compared to all other network models. [Conclusions] The experimental results showed that the UperNet-ESA semantic segmentation model proposed in this study outperforms the original network in terms of performance. The improved model achieves the best accuracy-speed balance compared to the current mainstream semantic segmentation networks. In the upcoming research, the current model will be further optimized and enhanced, while the kale dataset will be expanded to include a wider range of samples of nodulated kale leaf bulbs. This expansion is intended to provide a more robust and comprehensive theoretical foundation for intelligent kale field management. [ABSTRACT FROM AUTHOR]

【Objective】The study aimed to investigate the molecular mechanism of phenotype formation in kale cleavage leaves, and to investigate the role of BoLMI gene in split leaf formation.【Method】Using the DH line of split leaf kale as the test material, the complete cDNA sequence of the gene was successfully obtained using homologous cloning technology, and it was named BoLMI.【Result】The sequence analysis showed that the full-length of BoLMI was 531 base pairs and encoded 176 amino acids. The protein had a molecular weight of 20 789.35 Da and a theoretical isoelectric point of 7.24.According to Pfam conserved domain analysis, BoLMI protein contained a homeobox conserved domain. Phylogenetic tree analysis showed that the BoLMI of kale belonged to the same branch as that of Brassica napus and B.oleracea, and the genetic relationship was relatively close. BoLMI protein was a soluble protein located in the nucleus, containing one transmembrane domain and no signal peptide sequence. The qRT-PCR analysis showed that the expression level of BoLMI was higher that in the seedling stage of split leaf kale and lower that in the rosette stage. During the lotus stage of kale, the expression levels in different parts of the leaves of cracked leaf varieties were higher than those of round leaf varieties, with the highest expression level in the first leaf. There was a significant difference in expression levels between cracked leaf varieties and round leaf varieties.【Conclusion】It is speculated that the BoLMI gene plays an important role in the formation of split leaves in kale, providing a theoretical basis for accelerating kale leaf shape breeding. [ABSTRACT FROM AUTHOR]

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Natural amendments from agricultural waste to improve soil physicochemical properties continuously attract research interest in promoting eco-friendly plant production. The present study evaluated the proper use of sawdust, biochar, and compost made from farm waste for kale production from seedling propagation to field conditions. From the seedling propagation process, the results demonstrate that the most suitable growing medium for kale seedings was 0.5:1:1 v/v of sawdust + biochar + compost, which gave the fastest mean germination times (2.71 days) and the highest seed germination percentage (78.33%). In addition to investigating the selected growing media as the soil amendments at five different rates (0, 6.25, 12.50, 18.75, 25.00, and 31.25 t ha−1), the result reveals that the fresh weight of marketable leaves was significantly highest under the 31.25 t ha−1 treatment. The application rate that yielded the highest gross profit margins was eight times higher than the control. Moreover, in some harvesting periods, the kale leaf yields under the treatment of 31.25 t ha−1 showed higher total chlorophyll and carotenoid contents. [ABSTRACT FROM AUTHOR]

Microgreens are innovative vegetable products whose production and consumption are gaining popularity globally thanks to their recognized nutraceutical properties. To date, the effects of lighting conditions and growing substrate on the performances of Brassica carinata microgreens (indigenous to Africa) remain underexplored. The present study aimed at providing insights into the influence of different lighting treatments provided by LEDs, namely monochromatic blue (B), red (R), cool white (W) and a combination of three color diodes (B + R + W), and substrates (cocopeat, sand and cocopeat–sand mix (v/v) (1:1)) on the growth, yield and bioactive compounds of B. carinata microgreens. Seeds were germinated in dark chambers and cultivated in growth chambers equipped with LED lighting systems for 14 days under a fixed light intensity of 160 ± 2.5 µmol m−2 s−1 and photoperiod of 12 h d−1. The best performances were associated with the spectrum that combined B + R + W LEDs and with substrate resulting from the cocopeat–sand mix, including the highest yield (19.19 g plant−1), plant height (9.94 cm), leaf area (68.11 mm2) and canopy cover (55.9%). Enhanced carotenoid and flavonoid contents were obtained with B + R + W LEDs, while the B LED increased the total amount of chlorophyll (11,880 mg kg−1). For plants grown under B + R + W LEDs in cocopeat, high nitrate levels were observed. Our results demonstrate that substrate and light environment interact to influence the growth, yield and concentration of bioactive compounds of B. carinata microgreens. [ABSTRACT FROM AUTHOR]

In response to the current diversity of vegetables and the limitations of storage and preservation techniques caused by their propensity for spoilage and deterioration during transportation due to vigorous tissue metabolic activity, microbial activity, and moisture loss, this study focuses on the impact of High Voltage Electrostatic Field (HVEF) on the storage quality and metabolism of Chinese kale. Experimental data reveal that HVEF significantly inhibits respiration and reduces peak respiration during the storage of Chinese kale. It also effectively maintains storage quality attributes such as color, moisture, and chlorophyll content, thereby extending storage time. Further metabolomic studies show significant differences in the metabolite spectra between the HVEF-treated group and the control group, involving metabolites of vitamins, flavonoids, and alkaloids. Key metabolites such as valine, leucine, vitamin B6, ergonovine, thiamine, and β-lactose are significantly upregulated. In addition, enrichment pathway analysis indicates that HVEF significantly regulates the central carbon metabolism as well as the metabolism of alanine, aspartic acid, and glutamic acid. This study confirms the effect of HVEF in inhibiting the vitality of Chinese kale at the metabolic level. In conclusion, high-voltage electrostatic field treatment is considered a strategy that can effectively extend the storage period of Chinese kale and improve storage quality. This research provides a theoretical basis for the development of new vegetable storage preservation technologies. [ABSTRACT FROM AUTHOR]

Background: The vivid red, purple, and blue hues that are observed in a variety of plant fruits, flowers, and leaves are produced by anthocyanins, which are naturally occurring pigments produced by a series of biochemical processes occurring inside the plant cells. The purple-stalked Chinese kale, a popular vegetable that contains anthocyanins, has many health benefits but needs to be investigated further to identify the genes involved in the anthocyanin biosynthesis and translocation in this vegetable. Results: In this study, the purple- and green-stalked Chinese kale were examined using integrative transcriptome and metabolome analyses. The content of anthocyanins such as cyanidin-3-O-(6″-O-feruloyl) sophoroside-5-O-glucoside, cyanidin-3,5-O-diglucoside (cyanin), and cyanidin-3-O-(6″-O-p-hydroxybenzoyl) sophoroside-5-O-glucoside were considerably higher in purple-stalked Chinese kale than in its green-stalked relative. RNA-seq analysis indicated that 23 important anthocyanin biosynthesis genes, including 3 PAL, 2 C4H, 3 4CL, 3 CHS, 1 CHI, 1 F3H, 2 FLS, 2 F3'H, 1 DFR, 3 ANS, and 2 UFGT, along with the transcription factor BoMYB114, were significantly differentially expressed between the purple- and green-stalked varieties. Results of analyzing the expression levels of 11 genes involved in anthocyanin production using qRT-PCR further supported our findings. Association analysis between genes and metabolites revealed a strong correlation between BoGSTF12 and anthocyanin. We overexpressed BoGSTF12 in Arabidopsis thaliana tt19, an anthocyanin transport mutant, and this rescued the anthocyanin-loss phenotype in the stem and rosette leaves, indicating BoGSTF12 encodes an anthocyanin transporter that affects the accumulation of anthocyanins. Conclusion: This work represents a key step forward in our understanding of the molecular processes underlying anthocyanin production in Chinese kale. Our comprehensive metabolomic and transcriptome analyses provide important insights into the regulatory system that controls anthocyanin production and transport, while providing a foundation for further research to elucidate the physiological importance of the metabolites found in this nutritionally significant vegetable. [ABSTRACT FROM AUTHOR]

Kale is a nutrient-dense leafy vegetable associated with wide-ranging health benefits. It is tolerant of drought and temperature fluctuations, and could thus serve an increasingly important role in providing a safe and nutritious food supply during the climate crisis, while kale's ease of cultivation and ability to be grown in a wide range of soils make it a good fit for urban agriculture. In this pilot study we explored potential differences between kale grown at urban versus rural farms. We planted kale seedlings (Darkibor variety) at three urban and four rural farms in and around Baltimore City, Maryland, instructed farmers to cultivate them using their usual growing practices, harvested the kale from fields and points of distribution, and analyzed it for concentrations of carotenoids, vitamins C and K1, ten nutritional elements, and eight non-essential metals. Although sample sizes for some analyses were in some cases too small to produce statistically significant results, we identified potentially meaningful differences in concentrations of several components between urban and rural kale samples. Compared to urban samples, mean concentrations of carotenoids and vitamins were 22–38% higher in rural field samples. By contrast, mean concentrations for eight nutritional elements were higher in urban field samples by as much as 413% for iron. Compared to rural field samples, mean concentrations of nine non-essential metals were higher in urban samples, although lead and cadmium concentrations for all samples were below public health guidelines. Some urban-rural differences were more pronounced than those identified in prior research. For six elements, variance within urban and rural farms was greater than variance between urban and rural farms, suggesting urbanicity may not be the primary driver of some observed differences. For some nutrients, mean concentrations were higher than upper ranges reported in prior estimates, suggesting kale may have the potential to be more nutrient-dense than previously estimated. The nutritive and metals composition of this important crop, and the factors that influence it, merit continued investigation given its growing popularity. [ABSTRACT FROM AUTHOR]

Fermentation is used not only to preserve food but also to enhance its beneficial effects on human health and achieve functional foods. This study aimed to investigate how different treatments (spontaneous fermentation or fermentation with the use of starter culture) affect phenolic content, antioxidant potential, and cholinesterase inhibitory activity in different kale cultivars: 'Halbhoner Grüner Krauser', 'Scarlet', and 'Nero di Toscana'. Chosen samples were further tested for their protective potential against the Caco-2 cell line. HPLC-MS analysis revealed that the fermentation affected the composition of polyphenolic compounds, leading to an increase in the content of rutin, kaempferol, sinapinic, and protocatechuic acids. In general, kale cultivars demonstrated various antioxidant activities, and fermentation led to an increase in total phenolic content and antioxidant activity. Fermentation boosted anti-cholinesterase activity most profoundly in 'Nero di Toscana'. Extracts of spontaneously fermented 'Scarlet' (SS) and 'Nero di Toscana' (NTS) showed cytoprotective properties, as revealed by the malondialdehyde (MDA), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) assays. Additionally, strong anti-inflammatory activity of NTS was shown by decreased release of cytokines IL-1β and TNF-α. Collectively, the conducted studies suggest fermented kale cultivars as a potential source for functional foods. [ABSTRACT FROM AUTHOR]

Wheat stem saw fly (WSS) (Cephus pygmaeus L.) is a notorious pest causing significant yield reductions in wheat crop. The use of tolerant cultivars is the most important management tactic against WSS. However, the reactions of frequently cultivated hollow-stemmed bread (Triticum aestivum L.) and durum (Triticum durum Desf.) wheat cultivars to WSS in southeastern Anatolia region of Türkiye are unknown. This study investigated the reactions of hollow-stemmed bread and durum wheat cultivars (twelve each) to WSS in Diyarbakir and Adiyaman provinces in southeastern Anatolia region during 2021-22. The cultivars were tested under field conditions and data relating to number of infested stalks (NIS), infestation ratio (IR), stalk wall thickness (SWT), spike length (SL), number of grains spike-1 (NGS), grain weight spike-1 (GWS), grain yield (GY) and grain yield loss were recorded. The tested wheat cultivars significantly differed for the NIS and GY at both locations. Similarly, SWT, NGS, SL, and GWS significantly differed among cultivars and higher values of these traits were recorded for healthy stalks than infested stalks. Overall, bread wheat cultivars i.e., 'Kale' (7127 and 5642 kg ha-1), 'Dinç' (6788 and 5628 kg ha-1 and 'Hazar' (6249 and 5821 kg ha-1) and durum wheat cultivars i.e., 'Zühre' (6261 and 5272 kg ha-1) and 'Hasanbey' (6038 and 5141 kg ha-1) with higher SWT recorded the highest grain yield at Diyarbakir and Adiyaman locations. Yield losses varied from 2.5% to 5.3% for bread wheat and 1.57% to 8.39% for durum wheat cultivars at Adiyaman and Diyarbakir, respectively. It is recommended that wheat cultivars with high GY and SWT should be planted to lower WSS infestation and yield losses in the region and breeding solid stem cultivars. [ABSTRACT FROM AUTHOR]

The ornamental kale, scientifically known as Brassica oleracea var. acephala, stands out as an attractive plant admired for its vibrant leaves that grace the winter season. Notably, the hybrid KTOK-28-1 × KTOK-40-1 demonstrated the highest embryogenesis rate, yielding 56.12 embryos per Petri dish. This efficiency largely hinges on the characteristics of the donor plants used. Through flow cytometry analysis, approximately 61% of spontaneous doubled haploids (DH) were identified. These individuals exhibited normal flower development and successfully produced seeds when subjected to bud pollination. The compatibility index values exhibited variation among different DH lines, ranging from 1.0 to 13.12. This diversity is promising for the development of superior hybrids. Noteworthy among the DHs were KTDH-52, KTDH-56, and KTDH-57, exhibiting suitability for various horticultural traits. The emergence of these novel DH lines suggests their potential contribution to future breeding programs aimed at producing superior-quality hybrids. [ABSTRACT FROM AUTHOR]

Background: Alternaria brassicicola (Schw.) causes black spot disease, which is one of the major diseases limiting the production of vegetable crops, especially Chinese kale in Southeast Asia and Thailand. Previous pathogen management based on synthetic fungicides is expensive, toxic for humans and harmful to the environment. The current study investigated the efficiency of Talaromyces flavus (Klöcker) Stolk and Samson Bodhi001, Talaromyces trachyspermus (Shear) Stolk and Samson Bodhi002, Talaromyces flavus (Klöcker) Stolk and Samson Bodhi003, Neosartorya fischeri (Wehmer) Malloch and Cain Bodhi004 and Neosartorya fischeri (Wehmer) Malloch and Cain in controlling Chinese kale black spot disease caused by A. brassicicola under field conditions. Methods: A. brassicicola and four antagonistic fungal strains were cultured separately on a PDA plate and incubated at room temperature for 14 days. Ten mL of sterile water was poured into a culture plate and the spores were gently scraped from the mycelium with a sterile loop to obtain a spore suspension and afterwards adjusted to a final concentration of 106 spores mL-1. Result: The results showed that spore suspensions of 106 spores mL-1 of T. flavus Bodhi001, T. trachyspermus Bodhi002, T. flavus Bodhi003, N. fischeri Bodhi004 and N. fischeri effectively controlled black spot disease in field trials and resulted in a significant reduction in black spot incidence compared with the unprotected control. Meanwhile, the spore suspension of T. flavus Bodhi001 revealed the greatest suppression of black spot incidence, causing 10.23% and 42.93% disease reduction, compared with the negative control, indicating promising preventive activity against A. brassicicola. Based on our results, T. favus Bodhi001 is a promising biological control agent (BCA) in controlling A. brassicicola causing Chinese kale black spot disease. [ABSTRACT FROM AUTHOR]

Microgreens are vegetable or edible herb shoots harvested in the early stages of development. They have an important number of bioactive compounds and add color, texture, and flavor to dishes and salads. Given their benefits, small size, and high market prices, they can grow in indoor systems, where light is determinant. This study aimed to evaluate the effect of different light intensities on agronomic characteristics, color, chlorophylls and carotenoids content, and antioxidant activity represented by total phenolic content (TPC), eliminate, and antioxidant capacity (AC) in four Brassicaceae species in two colors (green and red). The experiment was conducted in a controlled light-emitting diode (LED) environment growth chamber (day/night temperatures of 25/20  ±  1.2°C, 16  h photoperiod, and 79  ±  2% relative humidity). Three light intensities were used for microgreen growth with the same LED light spectrum: low (120  ±  5.1  μmol  m-2 s-1 ), medium (160  ±  3.6  μmol  m-2 s-1 ), and high (210  ±  5.9  μmol  m-2 s-1 ). Eight  g of the seeds of green and red cultivars of cabbage, kale, mizuna, and mustard were sown in a plastic tray (64  cm  x  35  cm  x  6  cm) with a mixture of peat and perlite (1:2  =  v: v). Overall, the high intensity increased dry matter percentage and dry weight, except in green and red kale and green cabbage cultivars. In contrast, low intensity promoted a larger hypocotyl in all species than with high intensity; moreover, it enhanced the cotyledon area in green and red mizuna. Cabbage, kale, and mustard green cultivars were greener under medium intensity, whereas the low intensity enhanced the purple color of mizuna. In addition, chlorophyll a and b increased under low intensity in most species except the red kale and mustard cultivars. The high intensity raises the antioxidant activity, promoting a higher TPC and AC. The findings revealed that the light intensity generated variations in agronomic characteristics, color, chlorophyll content, and antioxidant activity of Brassicaceae microgreens, and the changes were based on the specific species and cultivars. [ABSTRACT FROM AUTHOR]

Simple Summary: Cabbage whitefly (Aleyrodes proletella) is a destructive sucking insect pest of brassica crops, particularly white cabbage and kale. Its importance has been increased over the last decade in many geographic regions, particularly in European countries. The control of cabbage whiteflies largely relies on the application of synthetic insecticides to protect yield if populations reach economic damage thresholds. One class of insecticides to control this pest are cyclic ketoenols targeting acetyl-CoA carboxylase (ACC), an enzyme involved in fatty acid biosynthesis. In 2019, reduced efficacy of ketoenol insecticides at recommended label rates were reported. Subsequently, we collected field samples of A. proletella in different European countries and confirmed the presence of ketoenol resistance in laboratory bioassays. The resistance allele was shown to be an autosomal dominant trait in crossing experiments between susceptible and resistant individuals. RNA sequencing and subsequent analysis revealed a mutation, an amino acid substitution, at the ketoenol binding site in ACC. The mutation has been previously functionally validated to confer high levels of ketoenol insecticide resistance in cotton whiteflies, too. A molecular screening of 49 populations revealed the presence of the mutations in several countries. We recommend the implementation of resistance management strategies for sustainable cabbage whitefly control. Cabbage whitefly, Aleyrodes proletella L., is an invasive hemipteran pest of cruciferous plants, particularly field brassica crops. Its importance has been increased over the last decade, particularly in European countries. The control of cabbage whiteflies largely relies on the application of synthetic insecticides, including tetronic and tetramic acid derivatives such as spiromesifen and spirotetramat (cyclic ketoenol insecticides), acting as insect growth regulators targeting acetyl-CoA carboxylase (ACC). In 2019, reduced efficacy against cabbage whiteflies of ketoenol insecticides at recommended label rates has been reported. Subsequently we collected field samples of A. proletella in different European countries and confirmed the presence of ketoenol resistance in laboratory bioassays. Reciprocal crossing experiments revealed an autosomal dominant trait, i.e., heterozygotes express a fully resistant phenotype. Transcriptome sequencing and assembly of ACC variants from resistant strains revealed the presence of an ACC target-site mutation, A2083V, as previously described and functionally validated in Bemisia tabaci (A2084V in A. proletella). Next, we employed a molecular genotyping assay to investigate the geographic spread of resistance and analyzed 49 populations collected in eight European countries. Resistance allele frequency was highest in the Netherlands, followed by Germany. Finally, we provide a proposal for the implementation of appropriate resistance management strategies. [ABSTRACT FROM AUTHOR]

The kefir‐based smoothies with kale and spinach were designed as a ready‐to‐drink and innovative functional snack. Microbiological, physicochemical, as well as pre‐ and postgastrointestinal total antioxidant capacity (TAC; CUPRAC, DPPH, and FRAP) analyses were conducted. It was determined that the kefir‐based smoothies with vegetables had higher ash, carbohydrate, and dietary fiber values. Fructose and glucose contents of smoothy with kale were high, while smoothy with spinach included high sucrose and maltose. The microbiology results revealed that kefir‐based vegetable smoothies had minimum Lactobacillaceae viability (>log 7 cfu g−1) for the required functional effect after 14‐day storage. Moreover, the addition of kale significantly increased (p <.01) the level of initial TAC (CUPRAC, DPPH, and FRAP) and total phenolic content (TPC) values. After in vitro gastric digestion analysis, smoothie with spinach demonstrated higher TAC and TPC values and the control sample had higher TAC and TPC values compared with a predigestion step. It was found that in vitro intestinal DPPH values were higher for the sample with spinach samples, while the sample with kale had the highest FRAP values. It was also found that the bioaccessibility indexes of plain kefir were determined to be the highest in both in vitro gastric and intestinal procedures. The present study provided novel insights into the in vitro digestion properties of kefir fortified with vegetables. Nevertheless, further studies are needed to identify the functional properties of the milk and plant matrices mixture using in vitro and in vivo trials. [ABSTRACT FROM AUTHOR]

Biochar and arbuscular mycorrhizal fungi (AMF), a promising environmentally friendly soil enhancer and biostimulant, play a crucial role in sustainable agriculture by influencing soil properties and plant growth. This research investigates the chemical properties of three biochar types [bamboo (BB-char), corn cob (CC-char), and coffee grounds (CG-char)] derived from different biomass sources and their impact on soil quality and Chinese kale growth. The results reveal significant differences in chemical properties among different types of biochar. Particularly, CG-char showed the greatest pH value and phosphorus content, with an average of 10.05 and 0.44%, respectively. On the other hand, CC-char had the highest potassium content, with an average of 2.16%. Incorporating biochar into degraded soil enhances soil structure, promoting porosity and improved texture, as evidenced by scanning electron microscope images revealing distinct porous structures. Soil chemistry analyses in treatment T2–T14 after a 42-day cultivation demonstrate the impact of biochar on pH, electrical conductivity, organic matter, and organic carbon levels in comparison to the control treatment (T1). Furthermore, the research assesses the impact of biochar on Chinese kale growth and photosynthetic pigments. Biochar additions, especially 5% BB-char with AMF, positively influence plant growth, chlorophyll content, and photosynthetic pigment levels. Notably, lower biochar concentrations (5%) exhibit superior effects compared to higher concentrations (10%), emphasizing the importance of optimal biochar application rates. The study also delves into the total phenolic content in Chinese kale leaves, revealing that the synergistic effect of biochar and AMF enhances phenolic compound accumulation. The combination positively influences plant health, soil quality, and nutrient cycling mechanisms. Overall, the research indicates the multifaceted impact of biochar on soil and plant dynamics, emphasizing the need for tailored application strategies to optimize benefits in sustainable agriculture. [ABSTRACT FROM AUTHOR]

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