1. Plant autophagy is responsible for peroxisomal transition and plays an important role in the maintenance of peroxisomal quality
- Author
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Michitaro Shibata, Kazusato Oikawa, Kenji Yamada, Kohki Yoshimoto, Maki Kondo, Yoshinori Ohsumi, Makoto Hayashi, Shino Goto-Yamada, Wataru Sakamoto, Shoji Mano, Mikio Nishimura, Department of Cell Biology, National Institute for Basic Biology [Okazaki], Department of Basic Biology, School of Life Science, The Graduate University for Advanced Studies, Department of Applied Biological Chemistry, Niigata University, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Bioscience, Nagahama Institute of Bio-Science and Technology, Institute of Plant Science and Resources, Okayama University, Frontier Research Center, and Tokyo Institute of Technology [Tokyo] (TITECH)
- Subjects
0106 biological sciences ,Arabidopsis thaliana ,Membrane lipids ,[SDV]Life Sciences [q-bio] ,Arabidopsis ,hydrogen peroxide ,01 natural sciences ,03 medical and health sciences ,Glyoxysome ,Autophagy ,Peroxisomes ,peroxisome ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,biology ,glyoxysome leaf peroxisome ,Cell Biology ,Peroxisome ,Plants, Genetically Modified ,biology.organism_classification ,pexophagy ,Autophagic Punctum ,Cell biology ,Plant Leaves ,Metabolic pathway ,Biochemistry ,chemistry ,Photorespiration ,peroxisome transition ,Reactive Oxygen Species ,Oxidation-Reduction ,Metabolic Networks and Pathways ,010606 plant biology & botany - Abstract
In photosynthetic cells, a large amount of hydrogen peroxide is produced in peroxisomes through photorespiration, which is a metabolic pathway related to photosynthesis. Hydrogen peroxide, a reactive oxygen species, oxidizes peroxisomal proteins and membrane lipids, resulting in a decrease in peroxisomal quality. We demonstrate that the autophagic system is responsible for the elimination of oxidized peroxisomes in plant. We isolated Arabidopsis mutants that accumulated oxidized peroxisomes, which formed large aggregates. We revealed that these mutants were defective in autophagy-related (ATG) genes and that the aggregated peroxisomes were selectively targeted by the autophagic machinery. These findings suggest that autophagy plays an important role in the quality control of peroxisomes by the selective degradation of oxidized peroxisomes. In addition, the results suggest that autophagy is also responsible for the functional transition of glyoxysomes to leaf peroxisomes.
- Published
- 2014