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Aim: Laparoscopic cholecystectomy is one of the treatment methods employed in symptomatic cholelithiasis cases, and pneumoperitoneum has been shown to increase oxidative stress. Our aim is to compare propofol and thiopental in terms of their effects on oxidative stress parameters in laparoscopic cholecystectomy cases. Materials and Methods: After obtaining Ethics Committee permission, patients who underwent laparoscopic cholecystectomy were divided into thiopental and propofol groups according to the agent used for anesthesia induction in the randomized and prospective study. Serum oxidative stress parameters levels were measured in all patients twice before induction of general anesthesia and at the 30th minute postoperatively. Results: When comparing the preoperative period with the postoperative period, total antioxidant status (TAS) levels increased statistically significantly in the propofol group (preoperatively 1.21±0.21 mmolH2O2/L, postoperatively 1.31±0.18 mmolH2O2/L, p<0.001) compared to the thiopental group (preoperatively 1.23±0.14 mmolH2O2/L, postoperatively 1.27±0.14 mmolH2O2/L, p=0.055). arylesterase (ARES), serum native thiol, total thiol, and disulfide levels decreased statistically significantly in both groups. While Paraoxonase-1 (PON1) level did not change in the propofol group, it decreased in the thiopental group. Conclusion: We concluded that propofol had a significantly greater oxidative stress reduction effect than thiopental. By increasing the TAS levels, propofol may have a more positive effect on oxidative stress than thiopental. However, neither propofol nor thiopental have oxidative stressreducing effects on other oxidative stress parameters. [ABSTRACT FROM AUTHOR]

Purpose: Due to the diverse biological activities attributed to its antioxidant, antidiabetic, anti-inflammatory, and cytoprotective properties, cyanidin-3-glucoside (Cy3G) has been widely studied. The present study was conducted to investigate the effects of Cy3G supplementation on redox balance disruption during acute exercise. Materials and Methods: To this end, 28 male Balb-C mice were divided into four groups: control, exercise, Cy3G, and exercise+Cy3G. During the experimental period, the mice in the Cy3G and exercise+Cy3G groups were administered 5 mg of Cy3G per kg of body weight, while the control and exercise groups were fed standard chow. The mice were trained on treadmill for 10 min every day at speed of 10 m/min for 2 weeks. On the 15th day, an acute exhaustion exercise was applied. Then, all groups were sacrificed, and serum samples were taken to analyze the native thiol, total thiol, disulfide, and thiol-disulfide indices. Results: The findings showed that the combination of Cy3G and exercise significantly increased native thiol levels and decreased disulfide levels compared to the other groups. However, it did not cause any change in total thiol levels. Native thiol were measured as 114.6±64.1, 106.4 ±57.9, 200.1±84.2, 262.1±105.9; Disulfide were determined as 253.2±71.6, 257.4±31.5, 213.8±44.8 and 188.4±32.2; Total thiol were 621.1±116.1, 621.2±100.1, 627.7±125.8 and 639.0±105.3 (respectively; control, exercise, Cy3G, and exercise+Cy3G). Conclusion: Cy3G supplementation can potentially maintain redox balance during acute exercise by supporting antioxidant defense systems and reducing oxidative stress. The use of Cy3G may have a positive effect on preventing exercise-induced oxidative stress and acute fatigue. [ABSTRACT FROM AUTHOR]

Objective: The main aim of this study was to investigate the differences in maternal serum thiol/disulfide homeostasis among women with abortion imminens (AI), missed abortion (MA), and healthy pregnancies during the first trimester. Materials and Methods: This was a prospective case-control study. This study was conducted on pregnant women who visited the Obstetrics Clinic at University of Health Sciences Turkey, Etlik Zübeyde Hanım Gynecology Training and Research Hospital and were diagnosed with either AI or MA during the 6th to 14th weeks of pregnancy. The participants had a normal pregnancy follow-up, no chronic illnesses, and did not take any multivitamin or antioxidant supplements except for folic acid. The study incorporated 33 pregnant women with AI, 36 with MA, and 40 with normal pregnancies. Age, and body mass index were matched across the three groups. This study used a recently developed automated spectrophotometric technique to quantify thiol/disulfide concentrations. Results: The AI group had considerably elevated levels of total thiol and native thiol (SH) compared with the MA group. Nevertheless, there was no notable disparity observed between the group of healthy pregnancies and the other two groups. Serum disulfide (SS) levels did not exhibit any significant variations among the three groups. Similarly, the ratios of SS/SH, SS/total thiol, and SH/total thiol did not show any significant differences between the groups (p>0.05). Conclusion: Patients with MA had decreased levels of total thiol and SH, which possess antioxidant capabilities, compared to the AI group. A decrease in antioxidant levels in the body may contribute to the etiology of MA. When considering our findings alongside existing literature, it remains inconclusive whether the serum thiol-disulfide ratio can predict a healthy pregnancy or MA following AI. Therefore, it is not yet seen as a promising diagnostic tool for assessing pregnancy viability. Additional investigation is required to establish the influence of dynamic thiol/disulfide homeostasis on early pregnancy loss. Amaç: Bu çalışmanın temel amacı, ilk trimester abortus imminens (AI), düşük ve sağlıklı gebeliği olan kadınlarda anne serumu tiyol/disülfit dengesindeki farklılıkları incelemektir. Gereç ve Yöntemler: Bu çalışma, Sağlık Bilimleri Üniversitesi, Etlik Zübeyde Hanım Kadın Hastalıkları Eğitim ve Araştırma Hastanesi Kadın Doğum Kliniği'ne başvuran, 6 ile 14. haftalar arasında AI veya düşük tanısı almış, normal gebelik süreci geçiren, kronik hastalığı bulunmayan ve folik asit dışında herhangi bir multivitamin veya antioksidan takviyesi kullanmayan hamile kadınları içeren prospektif bir çalışmadır. Çalışma, 33 AI, 36 düşük tanısı konmuş ve 40 normal gebelik olgusunu kapsamaktadır. Katılımcıların yaş, ve vücut kitle indeksi her üç grup arasında eşitlenmiştir. Tiyol/disülfit seviyeleri, yeni geliştirilmiş bir otomatik spektrofotometrik yöntemle ölçülmüştür. Bulgular: AI grubunda, düşük grubuna göre anlamlı derecede yüksek olan toplam tiyol ve doğal tiyol (SH) seviyeleri saptanmıştır. Ancak, sağlıklı gebelik gösteren grup ile diğer iki grup arasında belirgin bir fark bulunmamıştır. Serum disülfit (SS) düzeyleri üç grup arasında istatistiksel olarak anlamlı bir farklılık göstermemiştir. Ayrıca, SS/SH, SS/toplam tiyol ve SH/toplam tiyol oranları gruplar arasında önemli bir fark göstermemiştir (p>0,05). Sonuç: Düşük olgularında AI grubuna kıyasla düşük toplam tiyol ve SH seviyeleri gözlemlenmiştir; her ikisi de antioksidan özelliklere sahiptir. Vücuttaki antioksidan seviyelerindeki azalma, düşüğün etiyolojisine katkıda bulunabilir. Bulgularımızı mevcut literatürle birleştirdiğimizde, serum tiyol-disülfit oranının AI sonrasında sağlıklı bir gebeliği ya da düşüğü öngörüp öngöremeyeceği konusunda kesin bir sonuca varılamamıştır. Dolayısıyla, gebeliğin sağlıklı ilerleyip ilerlemeyeceğini değerlendirmede umut verici bir tanı aracı olarak görülmemektedir. Erken gebelik kaybında dinamik tiyol/disülfit homeostazının etkisini göstermek için ek araştırmalara ihtiyaç vardır. [ABSTRACT FROM AUTHOR]

Copyright of Ahi Evran Medical Journal is the property of Ahi Evran University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: To evaluate role of thiol and disulfide homeostasis, a novel marker of oxidative stress, in the follow-up acute abdomen. Material and Method: This prospective study included 107 patients (62 men and 45 women) with a diagnosis of acute abdomen (AA). In all patients, age, gender and cause of acute abdomen were recorded. In addition, native thiol (-SH), total thiol (tSH) and disulfide (-S-S-) levels at baseline, admission and on postoperative day 3 were prospectively recorded and -SS-/-SH, -S-S-/tH and -SH/tSH ratios were calculated. Results: When the causes of acute abdomen were assessed, it was seen that 72 patients (67.29%) underwent surgery due to appendicitis while 5 patients (4.67%) due to lower GIS perforation, 7 patients (6.54%) due to perforated peptic ulcer, 5 patients (4.67%) due to sigmoid volvulus, 4 patients (3.74%) due to strangulated hernia and 14 patients (13.08%) due to miscellaneous reasons. When thiol and disulfide levels were assessed as a single parameter, mean thiol level was 316.71±78.16 (327.5) at preoperative period and 264.00±72.85 (278.30) at postoperative period. The mean thiol level was significantly decreased at postoperative period (p

Backraund; Thiols are important elements for oxidation reactions and under oxidative stress. The aim of this study was to determine thiole levels, an antioxidative marker in CAD patients with stable and acute coronary syndrome. Methods; 210 of the patients included in the study were diagnosed with acute coronary syndrome (ACS), 205 consisted of patients with stable angina pectoris (SAP). Thiol groups levels and thiol/disulphide homeostasis was measured by spectrophotometrically. Results: Native thiol and total thiol levels, disulfide/natural thiol and disulfide/total thiol ratios were decreased in the ACS groups compared to the SAP groups Conclusions: Thiol levels and thiol / disulfide ratios can be used as markers to evaluate acute coronary syndrome.

Copyright of Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi is the property of Oguz KARABAY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tepecik Education & Research Hospital / İzmir Tepecik Eğitim ve Araştırma Hastanesi Dergisi is the property of Logos Medical Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This article aims to explain the altered oxidative status and thiol/disulfide homeostasis before and after surgery in children with congenital heart disease (CHD).

Aim: Polycythemia vera is a chronic myeloproliferative disease characterized by increased red cell mass and JAK2 mutation positivity. Transformation to myelofibrosis and acute leukemia is possible in patients with polycythemia vera. Oxidative stress causes DNA damage and might be a reason for malignant transformation. Thiol molecules can prevent the harmful effects of oxidative stress. Therefore, in this study, we aimed to analyze the state of thiol homeostasis in patients with polycythemia vera. Material and Methods: Thirty-one patients with polycythemia vera and 80 healthy volunteers were included in this study. Serum samples of the cases were stored until the end of the study. Native thiol, total thiol, disulfide, and ischemia modified albumin levels were determined. Results: The mean ischemia modified albumin (1.09±0.21 vs 0.67±0.08; p

Copyright of Turkish Journal of Trauma & Emergency Surgery / Ulusal Travma ve Acil Cerrahi Dergisi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Corona virus disease (COVID-19) is a global public health problem that has killed more than 4.5 million people. In this study, it was aimed to evaluate the relationship between the thiol and ischemia modified albumin (İMA) levels of COVID-19 patients treated in the inpatient and intensive care unit with their current clinical status. Materials and Methods: In this study, 300 patients who were confirmed to be positive by PCR test and diagnosed with COVID-19 infection were included. The patients were divided into two groups based on the unit they were treated. 160 of these patients were treated in the inpatient service (group 1) and 140 in the intensive care units (group 2). Thiol, İMA, CRP and ferritin levels were measured from the serum samples of the patients. Results: When the groups were compared, the thiol levels of group 2 were found to be significantly lower (p<0.001). In addition, it was observed that the İMA levels of group 2 were significantly higher than group 1 (p<0.001). When other laboratory tests were evaluated, the CRP and ferritin levels of group 2 were high (p<0.001) and the lymphocyte count was low (p<0.001). A significant correlation was found between thiol levels and CRP, ferritin, lymphocyte and İMA levels ( r=-0,58, p<0,001; r=-0,41, p<0,001; r=0,32, p<0,001; r=-0,39, p<0,001, respectively). In addition, a significant correlation was observed between İMA levels and CRP, ferritin, lymphocyte and thiol levels (r=0,19, p=0,02; r=0,18, p=0,043; r=-0,15, p=0,047; r=-0,39, p<0,001, respectively). Conclusions: The decrease in the level of thiol, which is a powerful antioxidant, in COVID-19 patients suggests that although it is due to the existing inflammatory process that causes oxidative stress, it may also be caused by the effect of oxidative stress due to ischemic conditions developing secondary to thrombosis. Therefore, the increase in İMA levels in intensive care patients in whom the inflammatory process and thrombotic events are observed more severely supports the decrease in thiol levels. As a result, the determination of thiol and İMA levels of COVID-19 patients treated in the inpatient and intensive care unit suggests that it has the potential to help with the current clinical status of the patients and the course of the disease. [ABSTRACT FROM AUTHOR]

Copyright of Archives of the Turkish Society of Cardiology / Türk Kardiyoloji Derneği Arşivi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi is the property of Oguz KARABAY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Archives of Clinical & Experimental Medicine is the property of Archives of Clinical & Experimental Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: Idiopathic Parkinson's Disease (PD) is a neurodegenerative disease caused by progressive neuronal degeneration in specific locations of the central nervous system. The organisms have developed complex antioxidant systems to regulate the effects and eliminate the damage of free radicals, which plays a role in the etiology of many neurodegenerative diseases. Oxidative stress is thought to play a pathophysiological role in PD. In this study, it was aimed to explore the role of dynamic thiol-disulfide homeostasis as an oxidative stress marker in PD pathophysiology as well as its relation with the characteristics of the disease. Material and Method: The study was conducted with 36 PD patients and 22 healthy individuals. Total Thiol (TT) (μmol/L) and Native Thiol (NT) (μmol/L) were measured, total thiols were mea- sured, and thiol/disulfide balance was detected as a new oxidative marker. Results: NT values were found lower in PD group (p<0.05). NT and TT levels were found in (-) relation with UPDRS scores similar to TT level relation with H-Y stage (p<0.05). Conclusion: Based on these findings indicating that thiol-disulfide homeostasis is disrupted in favor of the oxidative process, the importance of this mechanism in both PD pathophysiology and progress can be emphasized. [ABSTRACT FROM AUTHOR]

Copyright of Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi is the property of Oguz KARABAY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: Healthy life is sustained by maintaining the balance between oxidative and antioxidant homeostasis in the living organism. During oxidation process free radical products lead to cellular damage and death. It is well known that oxidative stress plays role in the etiology of many diseases. We aimed to study the relationship between oxidative stress and short term fasting by measuring the serum levels of ischemia modified albumin, thiol, and disulphide in pediatrics patients admitted for radiological imaging. Material and Method: Our study was carried out between April and November 2018. We included children between 6 months and 6 years. Children who were fasting for more than 5 hours were assigned as the study group. Children who were not fasting were assigned as the control group. Serum ischemia modified albumin, thiol and disulfide were calculated using the colorimetric method of Erel's. Results: In the study group, the mean± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.72± 0.12 ABSU, 559.15± 58.98 μmol/L, 606.83± 61.35 μmol/L and 23.83± 5.58 μmol/L respectively. In the control group, the mean ± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.77± 0.15 ABSU, 528.80± 54.45 μmol/L, 580.17± 63.41 μmol/L and 25.68± 8.07 μmol/L respectively. The disulphide /native thiol and disulphide / total thiol ratios were lower in the study group than the controls, but there were no significant differences between the two groups. Conclusion: Short term fasting does not change serum levels of oxidative stress biomarkers and dose not lead to inbalance in the oxidative stress hemeostasis in pediatric patients. [ABSTRACT FROM AUTHOR]

Purpose: We aimed to determine the alterations in thiol/disulphide homeostasis and ischemia-modified albumin (IMA) levels in patients with different subtypes of age-related cataract by comparing these values with control cases. Material and Method: Totally 198 eyes of 99 consecutive patients diagnosed with age-related cataract and 188 eyes of 94 control cases were included in the study. In cataract group disease subtypes were recorded as nuclear cataract, cortical cataract, posterior subcapsular cataract or mixed cataract. Results: Native thiol and total thiol levels were significantly lower in cataract group while disulphide, disulphide/native thiol and disulphide/total thiol ratios were significantly higher. IMA and adjusted IMA levels were also significantly higher in cataract group. When patients with different subtypes of cataract were analyzed; there was not any significant difference between patients with different subtypes of cataract regarding the thiol/disulphide homeostasis parameters or adjusted IMA levels. Conclusion: Serum dynamic thiol/disulphide homeostasis is altered in age-related cataract patients in the favor of oxidative stress and there was a significant increase in serum IMA and adjusted IMA levels in cataract patients. However, we did not determine any significant difference in dynamic thiol/disulphide homeostasis parameters or IMA levels between patients with different subtypes of cataract. Since age-related cataract is a common disease that can only be treated with surgery; to prevent or slow down its development is critical. The parameters of dynamic thiol/disulphide homeostasis or IMA may be regarded as new targets for the new treatment modalities in cataract. [ABSTRACT FROM AUTHOR]

Background: Thiol-disulphide homeostasis has vital role in cell signalling mechanisms, regulation of transcription factors and enzymatic activities, signal transduction and regulation of proliferation rate, apoptosis and detoxification and antioxidant protective mechanism. Objective: This study aims to demonstrate in Polycythemia Vera (PV) patients the thioldisulphide homeostasis which is known to play a role in cell proliferation, apoptosis and various steps of cell cycle. Design: Descriptive prospective cross-sectional study. Settings: Yildirim Beyazıt University Hospital Ankara, Turkey between 2016-2018. Method: Forty-two PV patients and 43 healthy controls were included in the study. Serum total (-SH + -S-S-) and native (-SH) thiol levels were measured in all subjects. The amount of dynamic disulphide bonds and, the ratio of (-S-S-) and (-S-S-) × 100/(- SH), (-S-S-) × 100/(-SH + -S-S-), and -SH ×100/(-SH + -S-S-) were calculated with automatic method. The data obtained from the patient group were compared with the control group. Main outcome measures: The amount of dynamic disulphide bonds and, the ratio of (-S-S-) and (-S-S-) × 100/(-SH), (-S-S-) × 100/(-SH + -S-S-), and -SH ×100/(-SH + -S-S-) were calculated with automatic method in PV patieint and healthy control group. Results: Both groups were similar in terms of age and gender distribution. Compared with the control group, PV group had significantly higher native thiol, total thiol and nativ/total thiol levels. Limitation: The generalizability of the study's findings were limited by the small sample size. Conclusions: In accordance with the nature of the disease, thiol balance in PV patients was in favor of proliferation. Increased total thiol (-SH + -S-S), native thiol (-SH) levels and native thiol/total thiol ratio might be associated with uncontrolled proliferation. The balance of the thiol-disulphide homeostasis shifted to reductive thiol side in the PV. This change can provocate proliferation status of the disease and/or may be secondary to the disease. Conflict of Interest: No conflict of interest was declared by the authors. [ABSTRACT FROM AUTHOR]

Aim: Oxidative stress is defined as the imbalance in the detoxification of antioxidants and reactive oxygen products produced during cellular metabolism. The deficiency of antioxidant leads to errors in oxidative hemostasis. Reactive oxygen products make damage to the macromolecules of the cell like lipid, protein and DNA and this leads to cellular death. Oxidative stress plays role in the pathogenesis of many chronic diseases. We aimed to investigate the relationship between short period sleep deprivation and thiol disulfide parameters of oxidative stress. Material and Method: Children between 6 months and 6 years were included in our study. Children with high fever, upper respiratory tract infection, diarrhea, vomiting, or who had any chronic infection or who use any drugs were excluded from the study. Children who were sleep deprived for more than 4 hours are assigned as the study group. Children who were not sleep deprived are assigned as the control group. Serum thiol and disulfide were calculated using the colorimetric method of Erel's. Results: Ninety five patients were included in the study. Fifty six (58.9%) were male and 39 (41.1%) were females. The median (IQR) age was 2.0 (2.0) year, the median (IQR) weight was 13.0 (7.0) kg. The median (IQR) period of sleeplessness was 8.0 (4.0) hours. There were no significant differences between the two groups according to serum levels of native thiol, total thiol, disülfide and disulfide/native thiol and disulfide/total thiol and native thiol/total thiol ratios. There was mild correlation between serum disulfide level and the sleep deprivation periods, but no statistical correlation between serum thiol levels and sleep deprivation periods. Conclusion: We conclude that the oxidative stres hemostasis was not affected by sleeplessnes in pediatric pateints. [ABSTRACT FROM AUTHOR]

Aim: This study was conducted to investigate thiol-disulphide homeostasis, a novel oxidative stress marker in primary restless legs syndrome (RLS) patients. Material and Method: Thirty-eight patients and 43 healthy subjects were included in the study. Serum native thiol, total thiol, disulphide and disulphide / native thiol percent ratio levels were measured by using a novel automated method. Diagnosis of RLS was based on the criteria proposed by the International Restless Legs Syndrome Study Group (IRLSSG), and RLS severity was assessed using the IRLSSG Severity Scale. Results: Serum native thiol and total thiol levels were significantly lower (p< 0.001; p= 0.007, respectively) while disulphide and disulphide/native thiol percent ratio levels significantly higher (p= 0.017; p< 0.001, respectively), in patients with RLS compared with control group. Significant correlations were found between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters (r= -0.56, p< 0.001 for native thiol; r= -0.51, p< 0.001 for total thiol; r= 0.37, p= 0.020 for disulphide and r= 0.60, p< 0.001 for disulphide/native thiol percent ratio). Conclusions: Decreased native thiol, increased disulphide levels and increased disulphide/native thiol percent ratio show that thiol-disulphide balance shifted to the oxidation side. Strong correlations between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters indicate that homeostasis may have a role in the pathogenesis of the Primary RLS. [ABSTRACT FROM AUTHOR]

Copyright of Archives of the Turkish Society of Cardiology / Türk Kardiyoloji Derneği Arşivi is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: We aimed to investigate the oxidative stress status in a population of women with untreated major depressive disorder. Methods: Fifty-four female patients with untreated major depressive disorder and 68 female healthy controls were included in the study. A Sociodemographic Form, the Hamilton Anxiety Rating Scale, and the Hamilton Depression Rating Scale (HAM-D) were applied to all the participants. Fasting blood samples were collected from all participants to assess serum thiol/disulphide levels and their pairwise ratios. Results: Native thiol levels were significantly higher and disulphide levels were lower in patients as compared to controls, while total thiol levels were not significantly different between the groups. Disulphide/native thiol and disulphide/total thiol ratios were significantly lower, while the native thiol/total thiol ratio was significantly higher, in the patient group than the control group. There was a negative correlation between HAM-D score and disulphide level, disulphide/native thiol ratio and disulphide/total ratio, while there was a positive correlation between HAM-D score and native/total thiol ratio, in the patient group. Discussion: This is the first study to investigate dynamic thiol/disulphide homeostasis in women with untreated major depressive disorder. Our results showed dynamic thiol/disulphide homeostasis shifts towards thiol formation which implies an antioxidant reaction in women with untreated major depressive disorder. [ABSTRACT FROM AUTHOR]

Metallothioneins (MT), participating in the stress response, and heavy metalbinding cysteine-rich small proteins. They protect cells the effects from oxidant and electrophilic molecules through sulfur group contents. For the discovery of functions of essential roles undefined MT proteins, studies are ongoing. They were firstly isolated and identified from horse kidney cortex proteins. Today they are present in all living organisms and have been determined to have common characteristics. The vital role of these proteins, such cadmium and mercury connect as the environmental toxic metals and zinc (Zn) and copper as well as the physiological significance of the metals are regulation of ionized forms. In the absorption of Zn, intracellular transport, mobilization, storage and transfer of Zn, which acts as a major regulator of MTs, have a major impact. The MTs have also important role in scavenge of free oxygen radicals. [ABSTRACT FROM AUTHOR]

Thiol is an organic compound that contain sulphhydryl group that have a critical role in preventing any involvement of oxidative stress in the cell. Among the protein defensive mechanism of the body, cysteine plays an important role in preventing an oxidative damage through its thiol functional group. These defensive functions are generally considered to be carried out by the low molecular weight thiol glutathione and by cysteine residues in the active sites of proteins such as thioredoxin and peroxiredoxin. In addition, there are thiols exposed on protein surfaces that are not directly involved with protein function, although they can interact with the intracellular environment. The process of protection of the cell against an oxidative damage occur by thiol and cystein residue that has a low molecular weight. These residue are present in the active sites of a protein like, peroxiredoxin and thioredoxin. Apart from intracellular antioxidant defense mechanism by protein thiol, there are presence of thiol in outer surface of protein that are not involved with the function of protein, even though they can interact with intracellular part of the cell. [ABSTRACT FROM AUTHOR]

Objective: The prevalence of Adult attention-deficit/ hyperactivity disorder (A-ADHD) has been estimated as to be around 5%. There are several hypotheses regarding the etiology of ADHD. Although numerous researches have been conducted regarding neurobiology of pediatric ADHD, A-ADHD studies were relatively few. Many studies have indicated that oxidant mediated neuronal damage may play a role in the pathophysiology of various psychiatric disorders. Recently, oxidative stress has been studied in A-ADHD and there is an accumulating evidence to support its' role. In the present study, we aimed to evaluate catalase (CAT) and thiol levels in A-ADHD patients and compare with healthy controls. Materials-Methods: Twenty-five A-ADHD patients from Gaziantep University, diagnosed according to Turgay's Turkish version of Adult ADD/ADHD DSM IV-Based Diagnostic Screening and Rating Scale by two psychiatrists, and 25 healthy volunteer controls were included. The subjects strictly refrained from any substance intake and physical exercise after 08:00 p.m. on the day before collection. CAT and Thiol levels were measured in plasma samples of study groups. Results: Age, gender, and body mass indexes of patients and controls have shown homogeneity and there were no differences between the groups. Total score and subscores were not correlated with any of the mentioned biochemical parameters. The mean CAT levels in patients with ADHD were significantly higher and Thiol levels were lower than those of controls (<0.001, <0.001, respectively). When the comorbid patient group compared with pure A-ADHD patients in terms of CAT (11.49±0.28,11.51 ±0.40, respectively) and thiol (0.22±0.05, 0.25±0.03, respectively) levels there were not found any significant differences (p=0.865, p=0.174, respectively). Conclusions: The results of the present study may indicate that thiol levels may be decreased with in reaction of increased CAT levels and thiol act like a pro-oxidant. This study may be considered as one of the initial phase studies that lighten the relationship between oxidative stress and A-ADHD. There is a need for further studies that will prove this relationship exactly. [ABSTRACT FROM AUTHOR]

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Amaç: Yüksek serum lipit düzeyleri ateroskleroz ve kardiyovasküler hastalıkların gelişimi için bağımsız risk faktörleridir. Hiperlipidemide oksidatif stres, endotel hasarı hızlandırır. Albumin, büyük plazma proteini plazmada bir antioksidan olarak çalışır. Oksidatif stres sırasında, plazma proteininin tiyol gruplarını ve albümin içindeki değişiklikleri iskemi modifiye albümin (IMA) olarak değerlendirebiliriz. Ürik asit, koroner arter hastalığının güçlü bir göstergesidir. Hiperlipidemi hastalarında ürik asit, IMA düzeyleri ve tiyol / disülfür evreni değişip değişmediği hipotezini öne sürüyoruz. Gereç ve Yöntem: Sağlıklı deneklerin ve hiperlipidemik hastaların serum örnekleri Ankara Atatürk Eğitim ve Araştırma Hastanesi'nde biyokimya laboratuarından alınmıştır. Sağlıklı bireylerde ve hiperlipidemik olgularda IMA, ürik asit ve tiyol / disülfit homeostazisi parametrelerini ölçtük. Bulgular: Kontrol grubu (n = 31), yaş ortalaması 47,7 ± 13,09 olan 17 kadın ve 18 erkek olup, hasta grubu (n = 37), 16 kadın ve 17'si ortalama yaş 49,8'dir. ± 11,04 yıl. Hiperlipidemik hastalarda nativ tiyoller azalır ve disülfit düzeyleri anlamlı olarak artar (sırasıyla p = 0.03 ve p = 0.02). HDL-kolesterol ve disülfür arasında anlamlı negatif korelasyon vardır (p = 0.001); HDL-kolesterol ve ürik asit (p = 0.007). Trigliserit ve disülfit düzeyleri arasında pozitif korelasyon bulundu (p <0.001). Sonuç: HDL-kolesterol, kardiyovasküler hastalıkta mortalitenin bir göstergesi olan ürik asit ile ters ilişkilidir. Ayrıca tiyol / disülfid homeostazında değişiklik ve trigliserit ve disülfür düzeyleri arasındaki pozitif korelasyon ve HDL-kolesterol ile disülfür ve HDL-kolesterol ve ürik asit arasındaki negatif korelasyon, hiperlipidemide kan proteininin antioksidan durumundaki bozulmayı göstermektedir. [ABSTRACT FROM AUTHOR]