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Antioxidant activity and anti-proliferative effect of a bioactive peptide from chickpea (Cicer arietinum L.).
- Source :
-
Food Research International . Nov2015, Vol. 77 Issue Part 2, p75-81. 7p. - Publication Year :
- 2015
-
Abstract
- Our previous study identified a chickpea peptide (CPe-III) derived from chickpea albumin hydrolysate which exhibited a high antioxidant activity. CPe-III-S peptide corresponding to amino acid sequences of CPe-III (RQSHFANAQP) was synthesized chemically to determine the bioactive activity. The reducing power, hydroxyl radical, DPPH radical, ABTS radical scavenging assay and DCFH-DA assay showed significant dose-dependent activities. The cell viability assay indicated a high inhibition activity on MCF-7 and MDA-MB-231 cells with EC 50 values of 2.38 and 1.50 μmol/mL. The p53 protein increased with increasing concentration of CPe-III-S tested by ELISA Kit. The specific binding of peptide and p53 protein was determined by means of molecular docking. These results indicated that CPe-III-S effectively inhibited the proliferation of breast cancer cells by increasing the p53. This is the first report of the synthetic peptide which based on CPe-III derived from the enzymatic hydrolysate of chickpea to exhibit the antioxidant activity and antiproliferative effect. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09639969
- Volume :
- 77
- Issue :
- Part 2
- Database :
- Academic Search Index
- Journal :
- Food Research International
- Publication Type :
- Academic Journal
- Accession number :
- 110702668
- Full Text :
- https://doi.org/10.1016/j.foodres.2015.09.027