ANALYSIS OF GARTANIN IN EXTRACT OF MANGOSTEEN PERICARP FRUIT(Garcinia mangostana L.)USING SPECTROPHOTOMETRIC FOURIER TRANSFORM INFRARED(FTIR) METHOD.

Gartanin is one of derivated xanthone compound in the pericarp of the mangosteen fruit. Gartanin has several pharmacological activities such as antifungal, antioxidant, anticancer bladder, and anticancer lung. The aims of this research are to analyze gartanin in the extract of mangosteen pericarp that combines with partial least squares multivariate calibration to determine the correlation between the concentration of gartanin in the extract of mangosteen pericarp that analyzes by HPLC and by FTIR spectroscopy. The wavenumbers region of 4000-650 cm-1 was selected for prediction of gartanin in the extracts. The correlation between actual values of gartanin determined by HPLC and FTIR predicted values using FTIR spectroscopy combined with PLS. The results showR2 values for each region are 0.988 and 0.981 for Subang and Tasikmalaya, respectively and for cross-validation values R2 = 0.838, PRESS = 0.0068898, and RMSEC = 0.0003192 for Subang and R2 = 0.969, PRESS = 0.0007422, dan RMSEC = 0.0002151 for Tasikmalaya. The high value of R2 and low value of RMSEC indicated the high accuracy and precision of FTIR spectroscopy for quantification of gartanin in the extracts. These results indicated that FTIR spectroscopy combined with PLS is an alternative technique for determination of gartanin in the extract of mangosteen pericarp (Garcinia mangostana L.). [ABSTRACT FROM AUTHOR]