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Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.
- Source :
-
Virology . Jun2019, Vol. 532, p22-29. 8p. - Publication Year :
- 2019
-
Abstract
- Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated entry of retroviral-, rhabdoviral and filoviral particles depends on CatB/CatL activity and that this effect is cell line-independent. Moreover, we show that the human cell line Calu-3, which expresses low amounts of CatL, is largely resistant to entry driven by diverse filovirus GPs. Finally, we demonstrate that Calu-3 cell entry mediated by certain filovirus GPs can be rescued upon directed expression of CatL or DC-SIGN. Our results identify Calu-3 cells as largely resistant to filovirus GP-driven entry and demonstrate that entry is limited at the stage of virion attachment and GP priming. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GLYCOPROTEINS
*CATHEPSIN B
*MARBURG virus
*CELL lines
*EBOLA virus
*ELASTASES
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 532
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 139233871
- Full Text :
- https://doi.org/10.1016/j.virol.2019.03.020