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Purification of stevia extract by chitosan precipitation and reversed‐phase chromatography.
- Source :
-
International Journal of Food Science & Technology . Jul2021, Vol. 56 Issue 7, p3409-3420. 12p. - Publication Year :
- 2021
-
Abstract
- Summary: To improve the shortness of the current purification technology of steviol glycosides (SG) extract, a two‐step purification procedure consisting of chitosan precipitation and reversed‐phase chromatography (RPC) was investigated. The impurity‐to‐SG ratio in the SG extract obtained from non‐destructive soaking was 5:1 ~ 6:1. It was found that 80.90% of the impurities could be removed by RPC using 20% (v/v) ethanol solution as the eluent with a high SG recovery of 96.98%. When 25% ethanol was applied, the SG recovery was reduced to 71.23%. An adsorption test using D280 anionic resin suggested that the impurities were negatively charged; thus, chitosan was used to co‐precipitate the impurities. An optimal decolourisation result of ~ 70% was obtained at 0.06 g·g−1 (w/w, to solids in extract) chitosan addition, shaking at 28 °C for 1 h. Afterwards, the extract was further purified by RPC. The total removal rate of impurities was increased to 91.20%, and the SG recovery was 85.15%. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEVIA
*CHROMATOGRAPHIC analysis
*STEVIOSIDE
*GLYCOSIDES
*ETHANOL
*CHITOSAN
Subjects
Details
- Language :
- English
- ISSN :
- 09505423
- Volume :
- 56
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- International Journal of Food Science & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 151314214
- Full Text :
- https://doi.org/10.1111/ijfs.14965