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Enzymatic synthesis of hydrophilic phytosterol polyol esters and assessment of their bioaccessibility and uptake using an in vitro digestion/Caco-2 cell model.
- Source :
-
Food Chemistry . Feb2022, Vol. 370, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • A novel enzymatic method for preparing phytosterol polyol esters was developed. • Phytosterol polyol esters possessed enhanced water solubility and thermal stability. • Bioaccessibility in vitro and cellular uptake of phytosterols were improved. A novel enzyme-catalyzed method was developed for the synthesis of phytosterol polyol esters from β -sitosterol and polyols (sorbitol, mannitol and xylitol) by two-step transesterification using divinyl adipate (DVA) as a link. A high conversion (exceeding 94%) of β -sitosterol with a vinyl group was achieved, in the presence of Candida rugosa lipase (CRL), at low temperature (35 °C) within 30 min. Subsequently, the maximum conversion of phytosterol polyol esters (>94%) was obtained using alkaline protease from Bacillus subtilis at 65 °C. Phytosterol polyol esters had enhanced thermal stability (up to an above 355 °C) and excellent water solubility (4.6–7.9 mM at 35 °C). Moreover, obvious increases in the bioaccessibility (41.5–63.6%) and intestinal uptake (5.2–6.5%) were observed using a simulated gastrointestinal digestion/Caco-2 cell model. These results highlighted the key role of hydrophilic structural modifications on physicochemical properties and absorption of phytosterols. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 370
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 153496335
- Full Text :
- https://doi.org/10.1016/j.foodchem.2021.131324