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A Sensitive and Selective Fluorimetric Method of Quick Determination of Sialic Acids in Egg Products by Lectin-CdTe Quantum Dots as Nanoprobe.
- Source :
-
Journal of Food Science (John Wiley & Sons, Inc.) . Dec2014, Vol. 79 Issue 12, pC2434-C2440. 7p. 1 Diagram, 5 Charts, 6 Graphs. - Publication Year :
- 2014
-
Abstract
- Sialic acids (SA) are widely found as components of oligosaccharide units in mucins, glycoproteins and other microbial polymers in nature food. The aim of this study is to create a new fluorimetric detection method applied for determinating SA in egg products by using a sensitive lectin-CdTe quantum dots (QDs) nanoprobe. Water-soluble and high luminescent CdTe QDs were conjugated with sambucus nigra bark lectin (SNA) as probe for SA detection. As a result of specific interaction between SA and SNA-CdTe QDs, the conjugations finally lead to the change of a fluorescent signal. Under optimal conditions, fluorescence intensity increase linearly with the increase of the concentration of SA ranging from 12 to 680 ng/mL. The low detection limit is 0.67 ng/mL. This quick and selective analysis method for SA detection has been used in synthetic samples and egg products with recovery between 97.92% and 110.42%, which demonstrates the application of this assay is feasible and practical. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00221147
- Volume :
- 79
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Food Science (John Wiley & Sons, Inc.)
- Publication Type :
- Academic Journal
- Accession number :
- 99973420
- Full Text :
- https://doi.org/10.1111/1750-3841.12706