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Natural hybrids from Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces kudriavzevii in wine fermentations.
- Source :
-
FEMS yeast research [FEMS Yeast Res] 2006 Dec; Vol. 6 (8), pp. 1221-34. - Publication Year :
- 2006
-
Abstract
- Several wine isolates of Saccharomyces were analysed for six molecular markers, five nuclear and one mitochondrial, and new natural interspecific hybrids were identified. The molecular characterization of these Saccharomyces hybrids was performed based on the restriction analysis of five nuclear genes (CAT8, CYR1, GSY1, MET6 and OPY1, located in different chromosomes), the ribosomal region encompassing the 5.8S rRNA gene and the two internal transcribed spacers, and sequence analysis of the mitochondrial gene COX2. This method allowed us to identify and characterize new hybrids between Saccharomyces cerevisiae and Saccharomyces kudriavzevii, between S. cerevisiae and Saccharomyces bayanus, as well as a triple hybrid S. bayanusxS. cerevisiaexS. kudriavzevii. This is the first time that S. cerevisiaexS. kudriavzevii hybrids have been described which have been involved in wine fermentation.
- Subjects :
- DNA, Fungal
DNA, Mitochondrial genetics
DNA, Ribosomal Spacer genetics
Electron Transport Complex IV genetics
Electrophoresis, Gel, Pulsed-Field
Fermentation
Genes, Fungal
Hybridization, Genetic
Karyotyping
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 5.8S genetics
Saccharomyces classification
Saccharomyces genetics
Saccharomyces cerevisiae classification
Saccharomyces cerevisiae genetics
Saccharomyces metabolism
Saccharomyces cerevisiae metabolism
Wine microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1567-1356
- Volume :
- 6
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- FEMS yeast research
- Publication Type :
- Academic Journal
- Accession number :
- 17156019
- Full Text :
- https://doi.org/10.1111/j.1567-1364.2006.00126.x