Method development and validation for analysis of microencapsulated cyanidin-3-O-rutinoside in dairy samples containing juçara palm fruit by high-performance liquid chromatography.

Euterpe edulis is a plant native of Brazil and popularly known as juçara. The aim of this study was to develop and validate an analytical method using high-performance liquid chromatography (HPLC) of juçara crude extracts (JCEs) and to evaluate dairy products containing free and microencapsulated extracts. The validated analytical method for JCE was performed using cyanidin-3-O-rutinoside (C3R - isolated and identified as major component from JCE) as a reference: linearity was obtained using ten concentrations between 25 and 250 μg mL ^-1 (R ²  = 0.994); limits of detection and quantification were 19.04 μg mL ^-1 and 57.7 μg mL ^-1 respectively; accuracy (relative standard deviation) was <5%; recovery was 104.85 μg mL ^-1 ; and the method is robust. Physicochemical analyses of the dairy products were evaluated using colour, pH, and concentration of anthocyanins using HPLC calculated as C3R over 21 days of storage. The pH remained stable until the end of the test. The colour and the concentration of anthocyanins remained stable for 9 days. C3R was isolated, the HPLC method was developed and validated, and the colour stability was not altered until 9 days. © 2023 Society of Chemical Industry.
(© 2023 Society of Chemical Industry.)