Effects of elevated CO2 concentration and temperature on the mixed-culture grown wild mustard (Sinapis arvensis L.) response to auxin herbicide.

Recently, there has been growing concern over the potential impact of CO₂ concentration and temperature on herbicide efficacy. The aim of the study was to examine the influence of single elevated CO₂ (400 vs. 800 ppm) and elevated CO₂ in combination with temperature (21 °C vs. 25 °C) on the effects of auxin herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) (0.5–2 × field recommended rate) to wild mustard (Sinapis arvensis L.) grown in mixed-culture with spring barley (Hordeum vulgare L.). MCPA had a detrimental effect on aboveground and belowground biomass, content of chlorophylls, enzymatic and non-enzymatic antioxidants and induced oxidative stress. The significant decline in photosynthetic rate, stomatal conductance and transpiration with MCPA dose was detected. Elevated CO₂ reinforced MCPA efficacy on S. arvensis: sharper decline in biomass, photosynthetic rate and antioxidant enzymes and more pronounced lipid peroxidation were detected. Under elevated CO₂ and temperature, MCPA efficacy to control S. arvensis dropped due to herbicide dilution because of increased root:shoot ratio, higher activity of antioxidants and less pronounced oxidative damage. Reinforced MCPA impact on weeds under elevated CO₂ resulted in higher H. vulgare biomass, while decreased MCPA efficacy under elevated CO₂ and temperature reduced H. vulgare biomass. [ABSTRACT FROM AUTHOR]