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Rapid Detection of Staphylococcus aureus in Food Using a Recombinase Polymerase Amplification-Based Assay
Rapid Detection of Staphylococcus aureus in Food Using a Recombinase Polymerase Amplification-Based Assay
- Source :
- Food Analytical Methods. 11:2847-2856
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Staphylococcus aureus (S. aureus) is of great importance and is a leading cause of food poisoning, which is a public health concern in terms of the frequency and seriousness of the disease. In the present study, RPA and real-time RPA assays were developed and validated to detect S. aureus with high sensitivity and specificity by targeting the nuc gene for the first time. The analytical sensitivity of real-time RPA was 102 copies/reaction, which was higher than the sensitivity of the real-time PCR method. The analysis time was reduced to 10 min, but this method was as reliable as real-time PCR. Furthermore, the potential use of RPA to detect S. aureus was validated with five different artificially contaminated foods. In conclusion, the RPA and real-time RPA assays developed here, similar to real-time PCR, are rapid and simple and exhibit with high sensitivity and specificity. These assays serve as efficient tools for the detection of S. aureus in less advanced laboratories and are suitable for point-of-care detection.
- Subjects :
- 0301 basic medicine
030106 microbiology
010401 analytical chemistry
Recombinase Polymerase Amplification
Biology
medicine.disease_cause
complex mixtures
01 natural sciences
Applied Microbiology and Biotechnology
Rapid detection
0104 chemical sciences
Analytical Chemistry
Microbiology
enzymes and coenzymes (carbohydrates)
03 medical and health sciences
Staphylococcus aureus
medicine
Pcr method
Safety, Risk, Reliability and Quality
Safety Research
Food Science
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi...........0052882d270f9dcec1ab5c712a25b3b6
- Full Text :
- https://doi.org/10.1007/s12161-018-1267-1