Azospirillum brasilense Exopolysaccharide Complexes, their Possible Involvement in Bacteria-Wheat Roots Interactions and the Suggested Nature of these Interactions

Two exopolysaccharide complexes hereafter termed lipopolysaccharide-protein complex and polysaccharide-lipid complex were isolated by means of washing-off with physiological solution from the cell surface of Azospirillum brasilense Spl07, Sp245, S17, 8, 15, 55, 75 and 80 of a complex biopolymer mixture followed by separation. On exposure to wheat seedling root hairs (Triticum aestivum cv. Saratovskaya 29) LPPCs and PSLCs were shown to cause root hair deformations similar to morphological changes of root hairs under the effect of bacterial cells. The data indicating the ability of complexes to bind to WGA, localized on wheat seedling roots were obtained by means of studying LPPCs and PSLCs inhibition in agglutination test under the effect of WGA. PSs containing GlcN, specifically interacting with WGA, were isolated from PSLCs.