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Fractional Extraction of Compounds from Grape Seeds by Supercritical Fluid Extraction and Analysis for Antimicrobial and Agrochemical Activities
- Source :
- Journal of Agricultural and Food Chemistry. 47:5044-5048
- Publication Year :
- 1999
- Publisher :
- American Chemical Society (ACS), 1999.
-
Abstract
- White grape seeds were subjected to sequential supercritical fluid extraction. By increasing the polarity of the supercritical fluid using methanol as a modifier of CO2, it was possible to fractionate the extracted compounds. Two fractions were obtained; the first, which was obtained with pure CO2, contained mainly fatty acids, aliphatic aldehydes, and sterols. The second fraction, obtained with methanol-modified CO2, had phenolic compounds, mainly catechin, epicatechin, and gallic acid. The fractions were bioassayed. Antimicrobial activities were checked on human pathogens, and a high degree of activity was obtained with the lipophilic fraction. Agrochemical activities on phytopathogenic fungi and activities on the etiolated wheat coleoptile bioassay were also checked. The more polar fraction was active in the latter bioassay. Keywords: Supercritical fluids; seeds; antimicrobial; extraction; grapes
- Subjects :
- Chromatography, Gas
Chromatography
Bacteria
Plant Extracts
Extraction (chemistry)
Fungi
Supercritical fluid extraction
food and beverages
Fraction (chemistry)
Microbial Sensitivity Tests
General Chemistry
Antimicrobial
Supercritical fluid
chemistry.chemical_compound
chemistry
Seeds
Humans
Bioassay
Biological Assay
Gallic acid
Phenols
Rosales
General Agricultural and Biological Sciences
Chromatography, High Pressure Liquid
Triticum
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....4f9d575dfd0189645f5bd5377dc579a5
- Full Text :
- https://doi.org/10.1021/jf990019p