Back to Search Start Over

Fractional Extraction of Compounds from Grape Seeds by Supercritical Fluid Extraction and Analysis for Antimicrobial and Agrochemical Activities

Authors :
Stephen J. Cutler
Larry T. Taylor
Rosa M. Varela
Horace G. Cutler
Miguel Palma
Source :
Journal of Agricultural and Food Chemistry. 47:5044-5048
Publication Year :
1999
Publisher :
American Chemical Society (ACS), 1999.

Abstract

White grape seeds were subjected to sequential supercritical fluid extraction. By increasing the polarity of the supercritical fluid using methanol as a modifier of CO2, it was possible to fractionate the extracted compounds. Two fractions were obtained; the first, which was obtained with pure CO2, contained mainly fatty acids, aliphatic aldehydes, and sterols. The second fraction, obtained with methanol-modified CO2, had phenolic compounds, mainly catechin, epicatechin, and gallic acid. The fractions were bioassayed. Antimicrobial activities were checked on human pathogens, and a high degree of activity was obtained with the lipophilic fraction. Agrochemical activities on phytopathogenic fungi and activities on the etiolated wheat coleoptile bioassay were also checked. The more polar fraction was active in the latter bioassay. Keywords: Supercritical fluids; seeds; antimicrobial; extraction; grapes

Details

ISSN :
15205118 and 00218561
Volume :
47
Database :
OpenAIRE
Journal :
Journal of Agricultural and Food Chemistry
Accession number :
edsair.doi.dedup.....4f9d575dfd0189645f5bd5377dc579a5
Full Text :
https://doi.org/10.1021/jf990019p