Polyphenols associated to pectic polysaccharides account for most of the antiproliferative and antioxidant activities in olive extracts

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Extracts rich in pectic polysaccharides associated to polyphenols were obtained from alperujo, the by-product of olive oil extraction, by different thermal treatments and subsequent chemical treatments. The resulting “Pectoliv” extracts exhibited strong antioxidant activities and a high antiproliferative capacities in vitro against colon carcinoma Caco-2 and leukemia monocytic THP-1 cell lines. Bleaching of the extracts with sodium chlorite removed the phenolic compounds and significantly reduced antiproliferative and antioxidant activities, confirming that these were due mainly to the associated polyphenols present in Pectoliv. However, even after bleaching, these activities remained very similar to that of a commercial citrus modified pectin obtained by chemical treatment from citrus pectin, which has been implicated in cancer treatment and prevention. Thus Pectolivs have a high potential as antiproliferative or antioxidant agents.
This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness and co-funded by a European Social Fund (ESF) [Ramon y Cajal Programme RyC 2012-10456; project AGL2013-48291-R and project AGL2016-79088-R]. A. Bermúdez-Oria received funding from the Spanish FPI program (MEIC) [BES-2014-068508]. The funding sources had no involvement in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.