1. A protective vaccine against the toxic activities following Brown spider accidents based on recombinant mutated phospholipases D as antigens
- Author
-
João Carlos Minozzo, Andrea Senff-Ribeiro, Luiza Helena Gremski, Giovana Scuissiatto de Souza, Ricardo Barros Mariutti, Ana Carolina Martins Wille, Rosangela Locatelli Dittrich, Nayanne Louise Costacurta Polli, Thaís Pereira da Silva, Fernando Hitomi Matsubara, Bruno Cesar Antunes, Hanna Camara da Justa, Silvio Sanches Veiga, Raghuvir K. Arni, Universidade Federal do Paraná (UFPR), State Department of Health, Universidade Estadual de Ponta Grossa (UEPG), and Universidade Estadual Paulista (UNESP) more...
- Subjects
Models, Molecular ,Gene isoform ,Necrosis ,Brown spider ,Spider Venoms ,Venom ,Phospholipase ,Biology ,Biochemistry ,Microbiology ,law.invention ,Leukocyte Count ,Mice ,Structure-Activity Relationship ,Immunogenicity, Vaccine ,Antigen ,Neutralization Tests ,Structural Biology ,law ,Brown Recluse Spider ,Spider Bites ,Phospholipase D ,medicine ,Animals ,Molecular Biology ,Loxoscelism ,Vaccines ,Antivenins ,Vaccination ,General Medicine ,Mutated phospholipases D ,medicine.disease ,Antibodies, Neutralizing ,Disease Models, Animal ,Treatment Outcome ,Accidents ,Recombinant DNA ,Mutant Proteins ,Rabbits ,medicine.symptom ,Biomarkers - Abstract
Made available in DSpace on 2022-05-01T09:47:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Universidade Federal do Paraná Fundação Araucária Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Accidents involving Brown spiders are reported throughout the world. In the venom, the major toxins involved in the deleterious effects are phospholipases D (PLDs). In this work, recombinant mutated phospholipases D from three endemic species medically relevant in South America (Loxosceles intermedia, L. laeta and L. gaucho) were tested as antigens in a vaccination protocol. In such isoforms, key amino acid residues involved in catalysis, magnesium-ion coordination, and binding to substrates were replaced by Alanine (H12A-H47A, E32A-D34A and W230A). These mutations eliminated the phospholipase activity and reduced the generation of skin necrosis and edema to residual levels. Molecular modeling of mutated isoforms indicated that the three-dimensional structures, topologies, and surface charges did not undergo significant changes. Mutated isoforms were recognized by sera against the crude venoms. Vaccination protocols in rabbits using mutated isoforms generated a serum that recognized the native PLDs of crude venoms and neutralized dermonecrosis and edema induced by L. intermedia venom. Vaccination of mice prevented the lethal effects of L. intermedia crude venom. Furthermore, vaccination of rabbits prevented the cutaneous lesion triggered by the three venoms. These results indicate a great potential for mutated recombinant PLDs to be employed as antigens in developing protective vaccines for Loxoscelism. Department of Cell Biology Federal University of Paraná (UFPR) Production and Research Center of Immunobiological Products (CPPI) State Department of Health Veterinary Hospital Federal University of Paraná (UFPR) Department of Structural Molecular Biology and Genetics State University of Ponta Grossa (UEPG) Multiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP) Multiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP) Universidade Federal do Paraná: 02/2020 Universidade Federal do Paraná: 04/2019 Fundação Araucária: 057/2017 CNPq: 303868/2016-3 CNPq: 408633/2018-2 more...
- Published
- 2021
- Full Text
- View/download PDF