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A study conducted at Auburn University focused on high-efficiency genome editing in naturally isolated strains of Aeromonas hydrophila and Edwardsiella piscicida using the CRISPR-Cas9 system. These bacteria are significant pathogens in aquaculture and can also cause infections in humans. The research aimed to develop tools for understanding pathogenic mechanisms and developing targeted strategies for control. The study successfully generated deletion mutants and insertion mutants in these pathogens, providing valuable tools for studying bacterial pathogenesis and developing effective control strategies. [Extracted from the article]

The article investigates carbapenemase-encoding genes in Burkholderia cepacia and Aeromonas sobria nosocomial infections among Iraqi patients. The study conducted in Ramadi Teaching Hospitals in 2024 found high antimicrobial resistance in isolates of B. cepacia and A. sobria, with a notable prevalence of blaKPC and blaGES carbapenemase producers. The research concluded that recA gene identification was a sensitive and specific technique for detecting B. cepacia complex isolates, and no isolates tested positive for the blaIMP gene. [Extracted from the article]

Researchers at the University of Guilan in Iran have developed a new in-silico approach to design a multiepitope peptide as a potential vaccine candidate for Aeromonas hydrophila, a Gram-negative bacterium commonly found in aquatic environments. The study aimed to address the challenges posed by antibiotic resistance in combating A. hydrophila infections by creating a vaccine candidate with high immunogenicity and safety profiles. The designed vaccine, consisting of 15 epitopes for B and T cells, showed promising results in virtual tests, indicating its potential effectiveness in combating A. hydrophila infections. Further research is needed to validate the safety, efficacy, and long-term impacts of this vaccine candidate. [Extracted from the article]

Research from Jilin Agricultural University in Changchun, People's Republic of China, focused on developing a phage lysate vaccine to combat infections caused by the Gram-Negative Bacteria Aeromonas hydrophila in crucian carp. The study optimized phage lysis conditions and compared immune responses of fish receiving the phage lysate vaccine to those receiving formaldehyde-inactivated vaccines. Results showed enhanced immunity in fish receiving the phage lysate vaccine, with a high protection rate of 88.89% in challenge tests post-immunization. This research contributes to the development of phage-based aquatic vaccines and was peer-reviewed. [Extracted from the article]

Researchers from Federation University Australia have highlighted the importance of monitoring the spread of resistance to polymyxin antibiotics, particularly through the mobilized colistin resistance gene mcr-3 found in the Aeromonas genus. This gene has been shown to increase resistance in naive E. coli and Aeromonas spp., posing a significant risk for the dissemination of resistance to polymyxins. The study delves into the genetic background of the mcr gene, the epidemiology of mcr-positive isolates, and the relationship between intrinsic and transferable mcr resistance genes, with a focus on mcr-3 and related genes. [Extracted from the article]

The article discusses the potential use of cyclic dipeptides derived from Aeromonas veronii V03 as antivirulence agents against Candida albicans infection. These dipeptides showed antibacterial activity against various pathogens and inhibited the virulence traits of C. albicans, such as yeast-to-hyphae transition, hydrolase secretion, and biofilm formation. The study suggests that these cyclic dipeptides could be developed into novel antifungal drugs to combat C. albicans infections. [Extracted from the article]

A recent study conducted at University Hospital Marques Valdecilla in Santander, Spain, focused on analyzing the virulence genes of Aeromonas species isolated from feces. The research aimed to compare the distribution of these genes between different species of Aeromonas and determine their presence in enterotoxigenic strains versus co-pathogen strains. The study found no significant differences in the distribution of virulence genes based on the co-existence of co-pathogens. A. hydrophila was identified as the potentially most virulent species among the strains studied. [Extracted from the article]

A study conducted at Hasanuddin University in Makassar, Indonesia, evaluated the use of CpG-ODN as an immunostimulant and protective agent against Aeromonas hydrophila in freshwater lobsters. The research found that CpG-ODN 2006 showed significant immunostimulant effects, while CpG-ODN 2133 exhibited potential as a protecting agent against the bacteria. These findings contribute to understanding immunostimulant strategies and protective mechanisms in freshwater lobsters, with potential implications for disease management in aquaculture. The study was published in the Bulletin of the European Association of Fish Pathologists and can be accessed online. [Extracted from the article]

Researchers from Southwest University in Chongqing, China have conducted a study on attenuated vaccines for Aeromonas veronii, an opportunistic pathogen that poses a threat to aquaculture and human health. The researchers constructed single and double gene deletion strains of A. veronii and found that the mutant strains had lower toxicity and decreased pathogenicity compared to the wild-type strain. They also evaluated the efficacy of one of the mutant strains as a live attenuated vaccine and found that it provided improved protection compared to an inactivated vaccine. These findings contribute to our understanding of A. veronii pathogenicity and provide a potential candidate for a live attenuated vaccine. [Extracted from the article]

A study conducted by researchers from the University of Barcelona and the Marine and Coastal Research Institute in Colombia has found potentially pathogenic bacteria in microplastics extracted from the Cienaga Grande de Santa Marta, the largest coastal lagoon system in the Colombian Caribbean. These bacteria form biofilms on the surface of microplastics, creating ecosystems known as plastispheres that are resistant to environmental changes. The presence of microplastics in fish consumed by the local population poses a risk to both the natural ecosystem and human health. The study highlights the need for integrated environmental management and public health strategies to address this issue. [Extracted from the article]

A study conducted by researchers at the University of Niigata in Japan explores the carbon storage regulator (Csr) system in Aeromonas salmonicida, a Gram-negative bacterium. The study found that the Csr system in A. salmonicida affects various phenotypes, including glycogen biosynthesis, biofilm formation, motility, and stability of small RNA molecules. The research highlights the conservation and functionality of the Csr system in A. salmonicida and raises questions about its regulatory targets and mechanisms. The study was supported by the Ministry of Education, Culture, Sports, Science and Technology, Japan. [Extracted from the article]

A new report from Liaquat National Hospital and Medical College in Karachi, Pakistan, discusses a case of necrotizing fasciitis (NF) caused by Aeromonas Hydrophila (AH) following laparoscopic surgery. The patient, a 72-year-old female with a history of hypertension, developed NF in the lower abdomen after laparoscopic cholecystectomy. Despite extensive surgical debridement and antibiotic treatment, the patient died from septic complications. This case highlights the need for further analysis of the behavior of such microbes in high-risk patients through collective case studies. This research has been peer-reviewed and published in the Journal of the Pakistan Medical Association. [Extracted from the article]

Researchers at the Chinese Academy of Agricultural Sciences have conducted a study on the use of an oral vaccine to prevent the spread of Aeromonas veronii in aquaculture. The study used a mutant form of the virulence factor aerolysin as a target antigen. The results showed that the vaccine effectively activated the immune response in zebrafish, increasing the expression of immune-related genes and antibodies. Additionally, the vaccine altered the structure of the gut microbiota. The study concludes that the vaccine could be an effective method for preventing A. veronii infection in fish aquaculture. [Extracted from the article]

A recent study conducted by researchers from Shandong University in China has investigated the molecular immune mechanism of macrophage response to Aeromonas veronii infection. Macrophages are innate immunity cells that play a crucial role in infectious immunity. The study found that macrophages infected with A. veronii released large amounts of cytokines and triggered NLRP3-dependent pyroptosis. Through integrated analysis of miRNA-mRNA, the study identified several pathways involved in macrophage response to A. veronii infection. The findings provide potential therapeutic targets for the prevention and treatment of A. veronii infectious diseases. [Extracted from the article]

Researchers from the Chinese Academy of Fishery Sciences have conducted a study on Aeromonas hydrophila, a bacterium that causes disease in frogs. The study found that two dominant bacteria, Aeromonas hydrophila Rd001 and Acinetobacter johnsonii Rd002, were isolated from naturally infected frogs. Experimental infection confirmed that Rd001 was the primary pathogen responsible for the disease. The study also identified the virulence genotype and antimicrobial susceptibility of Rd001, as well as its genetic traits. This research provides valuable information for preventing and controlling A. hydrophila infections in frogs. [Extracted from the article]

A research report from Jeonbuk National University in South Korea discusses the findings on Aeromonas, a type of Gram-Negative Bacteria. The study focuses on the transmission of antibiotic resistance genes (ARGs) in freshwater environments, particularly in relation to Aeromonas spp. The researchers conducted whole-genome sequencing on five environmental strains of Aeromonas spp. and identified conserved intrinsic resistance determinants called Aeromonas Qnr (AQnr) proteins. The study emphasizes the importance of understanding and monitoring these AQnr families to prevent the clinical emergence of antibiotic resistance. The research has been peer-reviewed and published in the Journal of Microbiology and Biotechnology. [Extracted from the article]

A rare case report and review of the literature from the Medical College of Nanchang University in China discusses the first case of super-refractory status epilepticus (SRSE) in a woman with Aeromonas caviae meningitis. The research highlights the lack of knowledge regarding this condition and emphasizes the importance of effective treatment for epilepsy in improving patient prognosis. The study suggests that a combination of one anesthetic drug and three non-anesthetic anti-epileptic drugs may be helpful in managing SRSE caused by Aeromonas caviae meningitis. This information is valuable for researchers and medical professionals studying and treating epilepsy and central nervous system infections. [Extracted from the article]

A study conducted by the University of Texas Medical Branch provides new insights into Aeromonas, a type of Gram-Negative Bacteria. The study analyzed 105 patients with Aeromonas infections and 112 isolates, examining their clinical presentations, outcomes, and antimicrobial resistance patterns. The research found that skin and soft tissue infections, traumatic wound infections, sepsis, burns, and intraabdominal infections were common. The study also identified high rates of antimicrobial resistance to carbapenems and piperacillin-tazobactam. The researchers concluded that tailored empirical therapy based on local antibiograms may lead to favorable outcomes. [Extracted from the article]

Researchers from the University of Stirling have published a new study on Aeromonas dhakensis, a bacterium that is increasingly recognized as an important pathogen in warm-water aquaculture and can also infect humans. The study highlights the need for accurate identification of A. dhakensis, as it may be more virulent than its close relative, Aeromonas hydrophila, and may pose zoonotic risks, including concerns for antibiotic-resistant strains. The researchers emphasize the importance of using reliable molecular tools for identification and call for further research to determine the true prevalence and impact of A. dhakensis in aquaculture, the environment, and healthcare settings. [Extracted from the article]

Researchers from the First Affiliated Hospital of Chongqing Medical University in China have published a case report on a 49-year-old female patient with multiple myeloma and renal failure who developed dysuria. The patient was found to have a urinary tract infection caused by multidrug-resistant Aeromonas caviae. After receiving treatment with piperacillin-tazobactam for seven days, her symptoms improved significantly. This case report aims to contribute to the diagnosis and management of Aeromonas caviae cystitis. [Extracted from the article]

A study conducted by Wenzhou Medical University in China examined the immune response of Procambarus clarkii (a type of crayfish) intestines to Aeromonas hydrophila bacterial infections. The researchers compared immune indexes, histopathological damage, and transcriptome data between infected and control crayfish. The results showed significant changes in intestinal tissue structure and enzyme activity, as well as differential gene expression related to cell processes and metabolic processes. The study provides important insights into the immune response of crayfish to A. hydrophila infection. [Extracted from the article]

Researchers at Emek Medical Center in Israel have conducted a study on Aeromonas species isolated from patients with gastroenteritis. The study aimed to investigate the antimicrobial susceptibility profiles of these isolates, explore the relationship between antimicrobial resistance (AMR) genes and resistance phenotypes, and describe the population structure of the isolates. The study found that A. caviae was the dominant species related to gastroenteritis and had a diverse population structure with limited evidence of common-source transmission. The resistance rates to most antimicrobial agents were low and partially correlated with the presence of resistance genes. [Extracted from the article]

A recent study conducted by researchers at Qingdao Agricultural University in China has focused on Vibrio vulnificus, a pathogen that causes skin ulcers and hemorrhagic septicemia in fish, leading to high mortality rates in aquaculture. The researchers developed a bivalent inactivated vaccine against both Vibrio vulnificus and Aeromonas salmonicida, another pathogen responsible for furunculosis in fish. The vaccine was found to be immunogenic and effectively prevented infection in turbot, a type of fish commonly affected by these diseases. The study suggests that this vaccine has potential applications in aquaculture. [Extracted from the article]

A new report discusses research findings on Yersinia enterocolitica, a bacterium that causes various human diseases, including gastroenteritis. The study focused on the immune response and antimicrobial susceptibility of macrophages infected with a strain of Aeromonas caviae and Yersinia enterocolitica recovered from the same fecal sample of a patient with diarrhea. The results showed that mixed infections caused more cell damage and overexpression of immune-related genes compared to single infections. Additionally, the study found an increase in antimicrobial resistance for certain antibiotics after infection with mixed cultures. This research provides insight into the impact of mixed infections on treatment and infection evolution. [Extracted from the article]

A study conducted by the University of California Los Angeles (UCLA) has found that plumbing issues and heat waves may be associated with nosocomial infections caused by carbapenemase-producing Aeromonas hydrophila. The research involved investigating a cluster of infections in two intensive care units, where patients with underlying comorbidities were infected with extensively drug-resistant (XDR) Aeromonas. The study identified diverse carbapenemase genes in the bacteria, highlighting the need for increased infection prevention measures during periods of plumbing problems, particularly in conjunction with heat waves. The research was published in the American Journal of Infection Control. [Extracted from the article]

Researchers from Fuyang Normal University in China have identified potential polyvalent passive vaccine candidates for Aeromonas hydrophila, a Gram-negative bacteria that affects fish. The study focused on the antibodies of outer membrane proteins (OMPs) of the bacteria, which can be obtained from egg yolk and have the potential to be used in aquaculture. The researchers found that certain IgY antibodies had an effective immune protection rate, activated innate immune responses, reduced inflammation and antioxidant reactions, protected tissue structure, and reduced apoptosis and DNA damage in infected kidney cells. The study suggests that IgY antibodies of specific OMPs can be considered as potential vaccine candidates for passive immunization in aquaculture. [Extracted from the article]

Researchers from the University of Putra Malaysia have conducted a study on the potential use of probiotics isolated from Pangasius nasutus, a type of fish, to combat infections caused by Aeromonas hydrophila and Streptococcus agalactiae. The study found that five probiotic strains inhibited the growth of A. hydrophila, with two strains also inhibiting the growth of S. agalactiae. The potential bacterial strains were identified as Lactococcus lactis, Weissella confusa, Cosenzaea myxofaciens, Lactococcus garvieae, and Plesiomonas shigelloides. Further research is needed to fully understand the mechanisms and potential of these strains as probiotics. The study was supported by a research grant from the Ministry of Education, Malaysia. [Extracted from the article]

Researchers at the Department of Medical Laboratory Technology have conducted a study on Aeromonas, a type of bacteria that can cause human diseases such as diarrhea and gastroenteritis. The researchers used Omphisa fuscidentalis larvae as an alternative model to study the virulence of Aeromonas. They found that injecting the bacteria into the larvae resulted in more severe symptoms at higher bacterial dosages. The study concluded that Omphisa fuscidentalis larvae can be used as an alternative model for studying bacterial infections, with survival percentage and melanization score as evaluation criteria. [Extracted from the article]

A new report from researchers at Srinakharinwirot University in Bangkok, Thailand discusses findings on the bacterium Aeromonas dhakensis, which is known to cause various human diseases. The researchers isolated and characterized four virulent phages that target A. dhakensis and found that bacterial numbers significantly decreased after treatment with these phages. Additionally, combining a phage cocktail with sub-MIC amoxicillin led to complete inactivation of the bacteria. The study suggests that using phages in combination with antibiotics could be an effective therapeutic approach. [Extracted from the article]

A research study conducted by the University of Rovira and Virgili in Reus, Spain, explores the potential use of the antihyperlipidemic drug fenofibrate for the management of Aeromonas infections. Fenofibrate, a fibric acid derivative, has been shown to have a protective effect against bacterial and viral infections in cell lines and in vivo models. In this study, fenofibrate-treated macrophages showed lower levels of cytotoxicity and intracellular bacteria compared to non-treated macrophages when infected with Aeromonas strains. However, further research is needed to determine the potential antibacterial effect of fenofibrate and the mechanism by which it provides protection. [Extracted from the article]

A new report from the University of Wisconsin Milwaukee provides fresh data on Aeromonas hydrophila, a bacterium that is becoming increasingly resistant to antibiotics. The study focused on a pathogenic strain of A. hydrophila that showed resistance to multiple antibiotics, including broad-spectrum and last-resort antimicrobials. Whole genome sequencing revealed the presence of a plasmid encoding 10 antibiotic resistance genes, including the first report of the beta-lactamase SFO-1 within a clinical strain of Aeromonas. The researchers suggest that this finding indicates the potential for antibiotic resistance gene mobility between different species of bacteria. [Extracted from the article]

A new report from researchers at Southwest University in Chongqing, China explores the virulence factors of the opportunistic pathogen Aeromonas veronii, which causes diseases in aquatic animals. The researchers focused on the gene tolC, which showed significantly different expression levels in two isolates of A. veronii with varying virulence. They constructed a mutant strain of A. veronii with a disrupted tolC gene and found that it exhibited reduced biofilm formation and inhibited response to certain antibiotics. Challenge tests showed that the mutant strain had a significantly higher LD50 than the wild-type strain, indicating reduced virulence. The researchers concluded that tolC is an important virulence factor and their findings provide references for the development of live-attenuated vaccines. [Extracted from the article]

Researchers at the University of Veterinary and Animal Sciences in Punjab, Pakistan have developed an oil-based inactivated vaccine for furunculosis caused by Aeromonas salmonicida in rainbow and brown trout. The vaccine was found to be effective in boosting the fishes' immunity, with a relative percentage of survival of 80% after challenge with the pathogen. The study provides evidence for the potential of vaccinations in combating bacterial illnesses in the fisheries and aquaculture industries. The research was supported by the Pakistan Science Foundation and has been peer-reviewed. [Extracted from the article]

Researchers from Imam Mohammad Ibn Saud Islamic University in Riyadh, Saudi Arabia, have developed an in silico multi-epitope vaccine against the bacterium Aeromonas hydrophila. This bacterium can cause various infections in humans and has shown resistance to many antibiotics. The researchers used artificial intelligence and machine learning techniques to design the vaccine, which targets the aerolysin toxin of the bacterium. In silico models predict that the vaccine will stimulate robust immune responses and is safe for administration. This research provides a potential solution to combat the high infection rates caused by A. hydrophila. [Extracted from the article]

Researchers at Diponegoro University have conducted a study on the antibacterial potential of sea cucumber Stichopus monotuberculatus symbiont bacteria against Aeromonas hydrophila and Vibrio harveyi, two pathogens that cause infections in aquaculture. The researchers isolated 16 bacteria, with 12 of them showing potential to inhibit A. hydrophila and 7 showing potential to inhibit V. harveyi. Through identification of 16S rRNA, the researchers found that Bacillus subtilis inhibited A. hydrophila, while Bacillus flexus inhibited V. harveyi. Both bacteria were found to have NRPS gene clusters. This research provides valuable insights into the potential use of sea cucumber symbiont bacteria in combating fish pathogens. [Extracted from the article]

A case report from the Mexican Institute of Social Security discusses a rare case of right-sided endocarditis caused by Aeromonas hydrophila, a type of bacteria. The patient was a 72-year-old female with a history of arterial hypertension and chronic kidney disease. She presented with symptoms of fever and a heart murmur, and blood cultures confirmed the presence of Aeromonas hydrophila. Treatment with intravenous fluoroquinolones led to resolution of the infection. This research provides new insights into the diagnosis and management of this uncommon condition. [Extracted from the article]

A research study conducted at Huzhou University in China has identified a strain of Aeromonas dhakensis as the cause of mass mortality in adult Macrobrachium rosenbergii, a type of freshwater prawn. The strain, named DKQ-1, was found to be highly pathogenic and capable of causing serious tissue damage in the prawns. The study also revealed that DKQ-1 was sensitive to 16 antibiotics, resistant to 9 antibiotics, and had intermediate resistance to spectinomycin and kanamycin. This research provides valuable information for the identification and prevention of bacterial diseases in M. rosenbergii caused by A. dhakensis. [Extracted from the article]

A recent study has identified a plasmid-encoded blaSFO-1 gene in a multi-drug resistant strain of Aeromonas hydrophila, a pathogenic bacterium. This gene confers resistance to a range of antibiotics, including cephalosporins, carbapenems, and fluoroquinolones. The study suggests that this is the first report of the blaSFO-1 gene in a clinical strain of Aeromonas, and highlights the potential for horizontal gene transfer between different bacterial species. However, it is important to note that this study has not yet undergone peer review. [Extracted from the article]

Researchers at Henan Normal University in Xinxiang, People's Republic of China, have conducted a study on the metabolic profiling of Cyprinus carpio (common carp) infected with Aeromonas hydrophila. The study found that myo-inositol, a metabolite with significant changes, may be a candidate biomarker for the host's anti-infection ability. Exogenous myo-inositol was shown to enhance the resistance of C. carpio against A. hydrophila infection, inhibit the inflammatory response, and alleviate oxidative damage to the liver. The study suggests that metabolic modulation could be a powerful strategy to improve therapy for bacterial infections. [Extracted from the article]

Researchers from Henan Normal University in China have conducted a study on the immune protection and safety of a live attenuated vaccine for Cyprinus carpio (common carp) against Aeromonas veronii, a pathogen that causes motile Aeromonas septicemia (MAS) in aquaculture. The study found that the fliD gene deletion strain of A. veronii was able to colonize temporarily in the fish without causing clinical symptoms or affecting growth. Vaccinated fish showed increased levels of IgM antibodies and an inflammatory response, indicating an effective immune response. The survival rates of vaccinated fish were significantly higher compared to the control group after challenge with wild-type A. veronii. The researchers concluded that the attenuated strain could be developed as an effective live attenuated vaccine to control A. veronii infection in Cyprinus carpio. [Extracted from the article]

A study conducted by the Chinese Academy of Fishery Sciences in Wuhan, China, focused on the characteristics of a dominant strain of Aeromonas bacteria found in sick red swamp crayfish. The researchers identified the strain as Aeromonas salmonicida subsp. masoucida, which causes red swamp crayfish head rot. The study found that the infected crayfish exhibited a strong immune response, as evidenced by the upregulation of immune-related genes. The research provides valuable insights into the emerging infectious diseases and immune response in red swamp crayfish. [Extracted from the article]

A study conducted by researchers from the Vellore Institute of Technology in Tamil Nadu, India, focused on the development of a bioactive feed for treating bacterial infections in freshwater prawns. The study found that the ethyl acetate extract of Bacillus licheniformis, a marine-based actinomycete, exhibited antibacterial and antioxidant properties. The researchers conducted an experimental trial with three groups of prawns, and the group that received the encapsulated B. licheniformis feed showed increased survival rates and enhanced antioxidant activities compared to the other groups. The study concluded that B. licheniformis demonstrated improved immune response, disease resistance, and gene expression in prawns challenged with Aeromonas hydrophila. [Extracted from the article]

A study conducted at Chang Gung Memorial Hospital examined the impact of cefuroxime susceptibility on the outcomes of Aeromonas necrotizing fasciitis (NF). The study found that despite aggressive antibiotic therapy and surgical debridement, Aeromonas NF can lead to high amputation and mortality rates. The research compared the antibiotic minimum inhibitory concentrations (MICs) between non-survivors and survivors of Aeromonas NF and found that non-survivors had a higher proportion of bloodstream infections, monomicrobial infections, shock, serous bullae, liver cirrhosis, chronic kidney disease, lower susceptibility to cefuroxime, and ineffective antibiotic prescriptions. The study concluded that experienced microbiologists can help physicians identify specific pathogens and test MIC to improve clinical outcomes and rational antimicrobial usage. [Extracted from the article]

A study conducted by the Chinese Academy of Fishery Sciences has analyzed the miRNA and mRNA transcriptome of the giant freshwater prawn Macrobrachium rosenbergii infected with Aeromonas hydrophila. The researchers identified differentially expressed miRNAs and mRNAs, as well as differentially predicted target genes. The study found that infection with A. hydrophila triggers complex responses in both miRNA and mRNA, significantly impacting immune and autophagy processes in M. rosenbergii. The findings suggest the involvement of various signaling pathways and functional categories related to immunity, apoptosis, and autophagy. [Extracted from the article]

A recent study conducted by researchers from Federal University Santa Maria in Brazil has examined the effects of dietary supplementation with R-(+)-limonene on silver catfish infected with Aeromonas hydrophila. The study found that R-(+)-limonene improved the growth, metabolism, stress, and antioxidant responses of the fish. The researchers concluded that R-(+)-limonene could be considered a beneficial dietary supplement for silver catfish. This research provides valuable insights into the potential use of R-(+)-limonene in aquaculture. [Extracted from the article]