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We investigated the optimal conditions for anthocyanin production in suspension cultures of a Euphorbia millii cell strain obtained by successive cell-aggregate selections. A modified Gamborg’s solution containing 5 to 7% sucrose (w/v), a 1:16 ratio of , 30 mm of nitrogen source, 10 6 m2,4-D, 10 8 m BA, and low concentrations of Fe2+ and greatly increased anthocyanin production. 2,4-D was superior to NAA in increasing anthocyanin production. In the anthocyanin production medium we established, E. millii cells produced 32 mg anthocyanin per liter per day.

A callus tissue culture was established from leaves and stems of Mentha spicata L. Lipid constituents isolated from callus tissues were composed of fatty acids, fatty acid methyl esters, triglycerides, squalene, stigrnasterol, sitosterol, oleanolic acid (1), ursolic acid (2) and pomolic acid (3). These liquid constituents did not contain monoterpenoids.

A computerized system that we designed allowed the fast tracing of the pedigree of cultured Euphorbia millii cells. We obtained two distinct outputs for which the parentage of the cells were printed. These outputs were obtained by two distinct search routines; one traced the pedigree from ancestry, and the other traced the pedigree from progeny. These outputs proved that pedigree tracing is useful for genetic studies of repetitive selection of cultured E. millii cells that produce high levels of anthocyanin.

Production d'acide usnique, bactericide utilise dans les infections de la peau, par des cultures de tissu de 2 especes de lichens