1. Transcription factor YY1 promotes adipogenesis via inhibiting CHOP-10 expression
- Author
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Qi Qun Tang, Hai Yan Huang, Chun Gu Ma, Tanjing Song, Xi Li, Mei Liu, and Qun He
- Subjects
Serum ,Leucine zipper ,medicine.medical_treatment ,Biophysics ,Biology ,Biochemistry ,Mice ,chemistry.chemical_compound ,Transactivation ,3T3-L1 Cells ,Adipocyte ,medicine ,Animals ,Insulin-Like Growth Factor I ,RNA, Small Interfering ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,YY1 Transcription Factor ,Regulation of gene expression ,ATF3 ,Adipogenesis ,Growth factor ,Cell Biology ,Cell biology ,Gene Expression Regulation ,chemistry ,embryonic structures ,Cancer research ,Cattle ,biological phenomena, cell phenomena, and immunity ,Transcription Factor CHOP - Abstract
CHOP-10, a dominant-negative member of the C/EBP family of transcription factors, is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPbeta through heterodimerization with its leucine zipper during 3T3-L1 preadipocyte differentiation. Our previous studies indicated that, FBS leads to the down-regulation of CHOP-10 expression after induction, and releasing C/EBPbeta from inhibitory constraint, allowing the transactivation of C/EBPalpha and PPARgamma genes, transcription factors required for terminal adipocyte differentiation. In the present study, we reported that FBS induced the expression of YY1, which bound to CHOP-10 promoter via two adjacent YY1-binding sites, suppressing its expression. The knock-down of YY1 expression with YY1 siRNA increased the expression of CHOP-10, inhibiting adipocyte differentiation. IGF-1, a growth factor present in greater concentration in FBS, independently induced the expression of YY1, and contributed to the down-regulation of CHOP-10 during the adipocyte differentiation program. Our studies suggested that YY1 can be a new adipocyte differentiation stimulator.
- Published
- 2008