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Your search keyword '"Hickman-Miller, Heather D."' showing total 3 results
Start Over Author "Hickman-Miller, Heather D." Journal biochemistry
3 results on '"Hickman-Miller, Heather D."'

1. Real time measurement of in vitro peptide binding to soluble HLA-A*0201 by fluorescence polarization

Author

Buchli, Rico, VanGundy, Rodney S., Hickman-Miller, Heather D., Giberson, Christopher F., Bardet, Wilfried, and Hildebrand, William H.

Subjects
Protein binding -- Research, Peptides -- Chemical properties, Histocompatibility testing, Biological sciences, Chemistry
Abstract

The development of a sensitive biochemical assay that relies upon fluorescence polarization is reported to indicate peptide interactions with recombinant soluble human leukocyte antigens (HLA) proteins. Testing of the A*0201-specific peptides with 48 additional class I molecules demonstrates that the unique peptide binding behavior of individual HLA molecules is maintained in the assay.

Published
2004

2. Development and validation of a fluorescence polarization-based competitive peptide-binding assay for HLA-A*0201--A new tool for epitope discovery

Author

Buchli, Rico, VanGundy, Rodney S., Hickman-Miller, Heather D., Giberson, Christopher F., Bardet, Wilfried, and Hildebrand, William H.

Subjects
Protein binding -- Observations, Peptides -- Structure, Peptides -- Chemical properties, Assaying -- Observations, Biological sciences, Chemistry
Abstract

A novel state-of-the-art biochemical HLA-A*0201 assay, which is comprised of technologically advanced cutting edge reagents is developed and validated to better facilitate epitope discovery. Results obtained demonstrated significant correlation among assay procedures, suggesting that the application of fluorescence polarization in combination with recombinant sHLA molecules is highly advantageous for the accurate assessment of peptide binding.

Published
2005

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