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Chemoselective and affinity-based probes of steviol were applied to the discovery of glycosyltransferase activity and corresponding UDP-glycosyltransferase in Arabidopsis thaliana, which illustrates a simple strategy for rapidly harnessing minable novel biological parts from plants for synthetic biology. [ABSTRACT FROM AUTHOR]

Gambogic acid has shown significant potential as an anti-cancer and anti-inflammatory compound; however, the molecular mechanism underlying the actions of gambogic acid, particularly its cellular target profiles, has not been investigated. In this study, we report the proteome-wide profiling of potential cellular targets of gambogic acid using quantitative mass spectrometry-based chemical proteomics. The target profiles of gambogic acid in living HeLa and K562 cells were identified, which strengthened our understanding of the mode of actions of the phytochemical. [ABSTRACT FROM AUTHOR]

We report a quantitative chemoproteomic approach that utilizes a clickable photoreactive probe for global profiling of celastrol targets, which may significantly improve the current understanding of celastrol's mode of action. [ABSTRACT FROM AUTHOR]

Selective profiling of steviol-catalyzing UDP-glycosyltransferases in plants was accomplished with a probe metabolically synthesized from two substrate-derived components comprising an alkynylated sugar receptor (steviol) module and a diazirine-modified sugar donor (UDP-glucose) module, thereby illustrating a facile approach for harnessing biosynthetic enzymes of natural glycosides in plants for synthetic biology. [ABSTRACT FROM AUTHOR]

CD147 is a glycosylated transmembrane protein highly expressed on the surface of various tumor cells which plays vital roles in tumor invasion, progression and metastasis. We report the discovery of the natural product pseudolaric acid B (PAB) directly targeting CD147 by chemical proteomics utilizing a PAB-derived photoaffinity probe which could serve as a novel type of anticancer reagent. [ABSTRACT FROM AUTHOR]