1. Combined VEGF and LMP-1 delivery enhances osteoprogenitor cell differentiation and ectopic bone formation.
- Author
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Wang, Xiuli, Cui, Fuai, Madhu, Vedavathi, Dighe, Abhijit S., Balian, Gary, and Cui, Quanjun
- Subjects
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VASCULAR endothelial growth factors , *PROTEINS , *CELL differentiation , *ECTOPIC tissue , *BONE growth , *NEOVASCULARIZATION , *RIBOSOMES - Abstract
A novel strategy to enhance bone repair is to combine angiogenic factors and osteogenic factors. We combined vascular endothelial growth factor (VEGF) and LIM mineralization protein-1 (LMP-1) by using an internal ribosome entry site to link the genes within a single plasmid. We then evaluated the effects on osteoblastic differentiation in vitro and ectopic bone formation in vivo with a subcutaneously placed PLAGA scaffold loaded with a cloned mouse osteoprogenitor cell line, D1, transfected with plasmids containing VEGF and LMP-1 genes. The cells expressing both genes elevated mRNA expression of RunX2 and β-catenin and alkaline phosphatase activity compared to cells from other groups. In vivo, X-ray and micro-CT analysis of the retrieved implants revealed more ectopic bone formation at 2 and 3 weeks but not at 4 weeks compared to other groups. The results indicate that the combination of the therapeutic growth factors potentiates cell differentiation and may promote osteogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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