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Your search keyword '"Kennelly, Edward J."' showing total 3 results
Start Over Author "Kennelly, Edward J." Topic momordica charantia Journal planta medica
3 results on '"Kennelly, Edward J."'

1. Transport in Caco-2 cell monolayers of antidiabetic cucurbitane triterpenoids from Momordica charantia fruits.

Author

Wu SB, Yue GG, To MH, Keller AC, Lau CB, and Kennelly EJ

Subjects
Biological Transport, Caco-2 Cells, Chromatography, High Pressure Liquid, Fruit chemistry, Glycosides chemistry, Glycosides isolation & purification, Humans, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Mass Spectrometry, Plants, Medicinal, Sterols chemistry, Sterols isolation & purification, Sterols metabolism, Triterpenes chemistry, Triterpenes isolation & purification, Diabetes Mellitus drug therapy, Glycosides metabolism, Hypoglycemic Agents metabolism, Momordica charantia chemistry, Triterpenes metabolism
Abstract

Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditional medicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cell monolayers, and were identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the basolateral side with apparent permeability coefficient (P app) values for 3-β-7-β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicines I and II at 9.02 × 10(-6), 8.12 × 10(-6), and 1.68 × 10(-6)cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo., (Georg Thieme Verlag KG Stuttgart · New York.)

Published
2014
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2. Cucurbitane-type triterpenoids from Momordica charantia.

Author

Ma J, Whittaker P, Keller AC, Mazzola EP, Pawar RS, White KD, Callahan JH, Kennelly EJ, Krynitsky AJ, and Rader JI

Subjects
Animals, Cell Line, Glycosides chemistry, Glycosides isolation & purification, Insulin Secretion, Mice, Nuclear Magnetic Resonance, Biomolecular, Saponins chemistry, Saponins isolation & purification, Triterpenes chemistry, Triterpenes isolation & purification, Insulin metabolism, Insulin-Secreting Cells drug effects, Momordica charantia chemistry, Saponins pharmacology, Triterpenes pharmacology
Abstract

One new cucurbitane-type triterpenoid glycoside, momordicoside U (1), together with five known cucurbitane-type triterpenoids and related glycosides, 3β,7 β,25-trihydroxycucurbita-5,23 (E)-dien-19-al (2), momordicine I (3), momordicine II (4), 3-hydroxycucurbita-5,24-dien-19-al-7,23-di-O-β-glucopyranoside (5), and kuguaglycoside G (6), were isolated from the whole plant of Momordica charantia. Their structures were determined by chemical and spectroscopic methods. Momordicoside U (1) was evaluated for insulin secretion activity in an in vitro insulin secretion assay and displayed moderate activity., (© Georg Thieme Verlag KG Stuttgart · New York.)

Published
2010
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3. Transport in Caco-2 Cell Monolayers of Antidiabetic Cucurbitane Triterpenoids from Momordica charantia Fruits.

Author

Shi-Biao Wu, Yue, Grace G. L., Ming-Ho To, Keller, Amy C., Lau, Clara B. S., and Kennelly, Edward J.

Subjects
DIET therapy for diabetes, CELL culture, FRUIT, RESEARCH methodology, MEDICINAL plants
Abstract

Bitter melon, the fruit of Momordica charantia L. (Cucurbitaceae), is a widely-used treatment for diabetes in traditionalmedicine systems throughout the world. Various compounds have been shown to be responsible for this reputed activity, and, in particular, cucurbitane triterpenoids are thought to play a significant role. The objective of this study was to investigate the gastrointestinal transport of a triterpenoid-enriched n-butanol extract of M. charantia using a two-compartment transwell human intestinal epithelial cell Caco-2 monolayer system, simulating the intestinal barrier. Eleven triterpenoids in this extract were transported from the apical to basolateral direction across Caco-2 cellmonolayers, andwere identified or tentatively identified by HPLC-TOF-MS. Cucurbitane triterpenoids permeated to the baso-lateral sidewith apparent permeability coefficient (Papp) values for 3-β-7-β,25-trihydroxycucurbita 5,23(E)-dien-19-al and momordicines I and II at 9.02 x 10-6,8.12 x 10-6, and 1.68 x 10-6 cm/s, respectively. Also, small amounts of these triterpenoids were absorbed inside the Caco-2 cells. This is the first report of the transport of the reputed antidiabetic cucurbitane triterpenoids in human intestinal epithelial cell monolayers. Our findings, therefore, further support the hypothesis that cucurbitane triterpenoids from bitter melon may explain, at least in part, the antidiabetic activity of this plant in vivo. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
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