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Objective: To study the risk factors of recurrence and metastasis in patients with breast cancer after modified radical mastectomy and the predictive value of serum carbohydrate antigen 125 (CA125), cyclo-oxygenase-2 (COX-2), soluble tumor necrosis factor receptor-P55 (sTNFR-P55). Methods: 109 patients with breast cancer who underwent modified radical mastectomy in The First Affiliated Hospital of Xinjiang Medical University from January 2014 to December 2016 were prospectively studied. All patients were followed up for 5 years after operation, including 2 patients who were lost to follow-up, and 107 patients who completed follow-up. According to the recurrence and metastasis within 5 years, the patients were divided into recurrence and metastasis group (n=31) and non-recurrence and metastasis group (n=76). The clinicopathological data of the patients at admission were collected. Preoperative serum CA125 was detected by electrochemiluminescence method, and preoperative serum COX-2 and sTNFR-P55 were detected by enzyme-linked immunosorbent assay. Logistic regression model was used to analyze the influencing factors of postoperative recurrence and metastasis, and receiver operating characteristic (ROC) curve was drawn to evaluate the predictive value of serum CA125, COX-2 and sTNFR-P55 on postoperative recurrence and metastasis. Results: The tumor diameter greater than 5cm, invasive non-specific carcinoma, vascular tumor thrombus, estrogen receptor (ER)/progesterone receptor (PR) negative, the constituent ratio of no endocrine therapy, TNM stage IIIA, and the number of axillary lymph node metastases 4~9 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group (P<0.05). The levels of serum CA125, COX-2 and sTNFR-P55 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group (P<0.05). Multivariate logistic regression analysis showed that tumor diameter greater than 5cm, invasive non-specific carcinoma, TNM stage IIIA, vascular tumor thrombus, number of axillary lymph node metastases 4~9, increased CA125, increased COX-2, and increased sTNFR-P55 were independent risk factors for recurrence and metastasis within 5 years after modified radical mastectomy (OR=1.318, 1.213, 1.223, 1.137, 1.257, 1.241, 1.313, 1.351, P<0.05). Serum CA125, COX-2 and sTNFR-P55 could effectively predict postoperative recurrence and metastasis of breast cancer, and the area under the curve (AUC) was 0.803, 0.749 and 0.761, respectively. The AUC of combined prediction of postoperative recurrence and metastasis of the three indexes was 0.915, and the sensitivity and specificity were 0.94 and 0.83, respectively. Conclusion: Tumor diameter, invasive non-specific carcinoma, TNM stage, vascular tumor thrombus, number of axillary lymph node metastases, and abnormal elevation of preoperative serum CA125, COX-2, sTNFR-P55 are risk factors for recurrence and metastasis within 5 years after modified radical mastectomy. Combined detection of preoperative serum CA125, COX-2 and STNFR-P55 can predict the risk of recurrence and metastasis after modified radical mastectomy. [ABSTRACT FROM AUTHOR]

Objective: To study the risk factors of recurrence and metastasis in patients with breast cancer after modified radical mastectomy and the predictive value of serum carbohydrate antigen 125 (CA125), cyclo-oxygenase-2 (COX-2), soluble tumor necrosis factor receptor-P55 (sTNFR-P55). Methods: 109 patients with breast cancer who underwent modified radical mastectomy in The First Affiliated Hospital of Xinjiang Medical University from January 2014 to December 2016 were prospectively studied. All patients were followed up for 5 years after operation, including 2 patients who were lost to follow-up, and 107 patients who completed follow-up.According to the recurrence and metastasis within 5 years, the patients were divided into recurrence and metastasis group (n=31) and non-recurrence and metastasis group (n=76). The clinicopathological data of the patients at admission were collected. Preoperative serum CA125 was detected by electrochemiluminescence method, and preoperative serum COX-2 and sTNFR-P55 were detected by enzyme-linked immunosorbent assay. Logistic regression model was used to analyze the influencing factors of postoperative recurrence and metastasis, and receiver operating characteristic (ROC) curve was drawn to evaluate the predictive value of serum CA125, COX-2 and sTNFR-P55 on postoperative recurrence and metastasis. Results: The tumor diameter greater than 5cm, invasive non-specific carcinoma, vascular tumor thrombus, estrogen receptor (ER) /progesterone receptor (PR) negative, the constituent ratio of no endocrine therapy,TNM stage ⅢA, and the number of axillary lymph node metastases 4~9 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group (P＜0.05). The levels of serum CA125, COX-2 and sTNFR-P55 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group (P＜0.05). Multivariate logistic regression analysis showed that tumor diameter greater than 5cm, invasive non-specific carcinoma, TNM stage ⅢA, vascular tumor thrombus, number of axillary lymph node metastases 4~9, increased CA125, increased COX-2, and increased sTNFR-P55 were independent risk factors for recurrence and metastasis within 5 years after modified radical mastectomy (OR=1.318, 1.213, 1.223, 1.137, 1.257, 1.241, 1.313, 1.351, P＜0.05). Serum CA125, COX-2 and sTNFR-P55 could effectively predict postoperative recurrence and metastasis of breast cancer, and the area under the curve (AUC) was 0.803, 0.749 and 0.761, respectively. The AUC of combined prediction of postoperative recurrence and metastasis of the three indexes was 0.915, and the sensitivity and specificity were 0.94 and 0.83, respectively. Conclusion: Tumor diameter, invasive non-specific carcinoma, TNM stage, vascular tumor thrombus, number of axillary lymph node metastases, and abnormal elevation of preoperative serum CA125, COX-2, sTNFR-P55 are risk factors for recurrence and metastasis within 5 years after modified radical mastectomy. Combined detection of preoperative serum CA125, COX-2 and STNFR-P55 can predict the risk of recurrence and metastasis after modified radical mastectomy. [ABSTRACT FROM AUTHOR]

Objective: To study the expression of N-myristoyltransferase 1 (NMT1) and 2 (NMT2) in breast cancer tissues and their biological effects on breast cancer cells. Methods: The expression of NMT1 and NMT2 in 40 breast cancer tissues were detected by ELISA, and their expressions were confirmed by immunohistochemistry. Small RNA interference technology knocked down the expression levels of NMT1 and NMT2 in breast cancer cells MCF-7 and BT-474. CCK-8 and Transwell chamber transmembrane assays were used to detect the changes of cell proliferation and metastasis before and after NMT1 and NMT2 knockdown. Results: The expressions of NMT1 and NMT2 in breast cancer tissues of patients with lymph node metastasis and stage III/IV were significantly increased (P<0.01). Using CCK-8 detection, it was found that the proliferation activity of breast cancer cells BT-474 and MCF-7 after NMT1 or NMT2 knockdown for 48 h was significantly lower than that of control cells (P<0.0001). Transwell chamber penetration test showed that the number of transmembrane cells in the NMT1 or NMT2 knockdown group was significantly lower than that in the control group (P<0.01). Conclusion: NMT1 and NMT2 play a key role in the occurrence and development of breast cancer. Knockdown of NMT1 and NMT2 can weaken the proliferation and metastasis of Breast cancer cancer cells. Targeted inhibition of NMT1 and NMT2 is expected to become an important molecular target for intervention in breast cancer. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the value of preoperative detection of carbohydrate antigen 125 (CA125), osteopontin (OPN), chemokine ligand 8 (CXCL8) and neutrophil lymphocyte ratio (NLR) in evaluating recurrence and metastasis risk of breast cancer patients undergoing modified radical mastectomy. Methods: 384 patients with modified radical mastectomy whow ere treated in our hospital from April 2015 to April 2016 were divided into non recurrent metastasis group (n=345) and recurrent metastasis group(n=39) according to postoperative recurrence and metastasis. Compared with CA125, OPN, CXCL8 and NLR in relapse metastasis group and non recurrence metastasis group, multivariate regression analysis was used to analyze the influencing factors of recurrence and metastasis after modified radical mastectomy. The receiver operating characteristic (ROC) curve was used to determine the value of CA125, OPN, CXCL8 and NLR in evaluating the recurrence and metastasis risk of breast cancer patients undergoing modified radical mastectomy. Results: The levels of CA125, OPN, CXCL8 and NLR in recurrence and metastasis group were higher than those in non recurrence and metastasis group(P<0.05). Postoperative recurrence and metastasis of modified radical mastectomy were related to tumor diameter, clinical stage, preoperative neoadjuvant chemotherapy, human epidermal growth factor receptor 2 (HER2), lymph node metastasis, histological type, cell proliferation marker antigen (Ki-67), estrogen receptor(ER) / progesterone receptor (PR), P53, postoperative radiotherapy, postoperative endocrine therapy (P<0.05). Multivariate Logistic regression analysis showed that OPN was high, CXCL8 was high, NLR was high, tumor diameter was greater than 2 cm, lymph node metastasis was positive, ER/PR was double negative, clinical stage was III stage, and preoperative neoadjuvant chemotherapy was not a risk factor for recurrence and metastasis of breast cancer after modified radical mastectomy (P<0.05). The area under the curve (AUC) of preoperative CA125, OPN, CXCL8 and NLR was 0.855, which was higher than that of each index. Conclusion: The recurrence and metastasis of breast cancer after modified radical mastectomy are related to OPN, CXCL8, NLR, tumor diameter, lymph node metastasis, ER/PR, clinical stage and preoperative neoadjuvant chemotherapy. The combined detection of CA125, OPN, CXCL8 and NLR before operation is of high value in evaluating postoperative recurrence and metastasis. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between preoperative MRI signs and vascular endothelial growth factor (VEGF) in breast cancer, and to provide reference for the prediction of VEGF. Methods The preoperative MRI of 73 patients with breast cancer was retrospectively analyzed. The patients were divided into VEGF over expression (28) and VEGF low expression (45) groups. Clinicopathological and preoperative MR data of the two groups were analyzed by univariate analysis, and a multivariate diagnostic model was established by logistic regression equation. Independent predictors of the model for the diagnosis of VEGF in breast cancer were calculated. Results The results of univariate analysis showed that the expression of VEGF in breast cancer was closely related to the molecular subtype, histological grade and lymph node metastasis of breast cancer (P<0.05). The expression of VEGF was not correlated (P>0.05) with the type of tumor, apparent diffusion coefficient value and time signal curve, but was closely related to tumor diameter, ring enhancement, intratumoral hyperintensity and peritumoral edema (P<0.05). Multivariate logistic :regression analysis showed that histological classification (OR=I0.296, P=0.005), peripheral rim enhancement {OR=I0.819, P=0.030), and peritumoral edema (OR= 15.297, P=0.012) were independent predictors of VEGF in breast cancer (Hosmer-Lemeshow X²=S.224, P=0.733). Conclusion The expression of VEGF is closely related to many malignant biological behaviors of breast cancer. Preoperative MRI sign of peripheral rim enhancement and peritumoral edema can be independent predictor of VEGF expression in breast cancer. [ABSTRACT FROM AUTHOR]

Objective: To study Comparison of the diagnostic value of Doppler ultrasonography and 128-slice spiral CT in breast cancer with axillary lymph node metastasis. Methods: A retrospective analysis was performed on 60 cases of breast cancer confirmed by surgery and pathology from March 2017 to March 2019 in our hospital. To compare the detection rate, sonographic characteristics, sensitivity, specificity and accuracy of Doppler ultrasonography and 128-slice spiral CT in axillary lymph node metastasis of breast cancer. Results: Among the 60 patients with breast cancer confirmed by pathology after surgery, 38 had axillary lymph node metastasis and 22 had no axillary lymph node metastasis. In the diagnosis of breast cancer with axillary lymph node metastasis, 36 cases were confirmed by Doppler ultrasound, and 30 cases were confirmed by 128-slice spiral CT. The detection rate of cortical centripetal growth, intra-lymph node calcification, lymph node diameter ratio and lymph node boundary ambiguity by Doppler ultrasound was significantly higher than that by 128-slice spiral CT (P<0.05). Use pathology as the gold standard. The sensitivity, specificity and accuracy of Doppler ultrasound were higher than those of 128-slice spiral CT, and there were significant differences between the two groups (P<0.05). Conclusion: Doppler ultrasonography is of high diagnostic value in breast cancer with axillary lymph node metastasis, which can help to provide correct diagnosis and select appropriate treatment. [ABSTRACT FROM AUTHOR]