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Copyright of Journal of Tea Science is the property of Journal of Tea Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective) The present paper aimed to obtain the complete sequence of SsREMO-la gene, conduct tissue-specific expression, qPCR expression analysis, sub-cellular localization study, and the phenotypic changes of Arabidopsis thaliana with SsREMO-la gene transfer and the expression pattern of CAT-1 gene. (Method) The complete sequence of SsREMO-la gene was obtained by PCR, purification, cloning and sequencing from Hainan sugarcane wild species (Sp-24). Drought stress was applied to the normal growth of the seedlings. Young leaves, roots and stems at 0, 1, 2, 3, 4 and 5 days after the stress treatment were collected for tissue-specific expression analysis and qPCR analysis. The tissue location and function of SsREMO-1a were analyzed by tobacco sub-cellular mapping and gene transformation techniques. PB1221-SSREMO-1a-GFP fusion expression vector was constructed and transformed into tobacco for cell localization analysis. The over-ex- pression vector pCAMBIA 1304-SsREMO-la was constructed, and SsREMO-1a was introduced into A. thaliana by agrobacterium transformation. The phenotypes of plants at 0, 1, 2, 3, 4 and 5 days after drought stress and the expression of CAT-1 gene were observed. [Result] The full length of SsREMO-1a gene was 738 bp, and the open code frame (ORF) was 564 bp, encoding 187 amino acids. Tissue-specific expression showed that SsREMO-1a was expressed in roots, stems and leaves. qPCR results showed that the gene was induced by drought stress and showed significantly up-regulated expression. The results of sub-cellular localization showed that SsREMO-1a protein was localized on the cell membrane. A total of 11 transgenic A. thaliana lines were obtained, and the drought resistance identification showed that the transgenic plants could improve the drought resistance of the plants. The expression of CAT-1 gene in transgenic plants was significantly in- creased. [Conclusion] SsREMO-1a can improve the drought resistance of plants and regulate CAT-1 gene to some extent. [ABSTRACT FROM AUTHOR]

To clarify the codon usage characteristics of CqGAI gene, the sequence of CqGAI gene was cloned. The codon usage preference of CqGAI gene was analyzed using CodonW, SPSS software and EMBOSS online program, and neutral mapping, ENC analysis and parity preference bias analysis were performed with GAI genes of 25 plants. The results showed that the length of coding sequence (CDS) of CqGAI gene was 1 782 bp, which encoded 593 amino acids, and contained the unique structural domains of DELLA gene family such as DELLA, TVHYNP, NLS, VHIID, LHR and RVER. The CqGAI gene could rapidly responded to gibberellines (GA), which played a key role in the GA signaling pathway. Codon bias analysis showed that ENC, CAI and GC content of CqGAI gene were 54.14, 0.21 and 46.18%, respectively, with weak codon bias, preference ending with A/T and 27 high frequency codons. The clustering analyses showed that the CqGAI gene had the closest preference to Caryophyllales. The base composition and correlation analysis revealed that the codon preference of CqGAI gene was influenced by selection effect and base mutation. The frequency of codon usage indicated that both Escherichia coli and yeast were suitable for heterologous expression of CqGAI gene, and Arabidopsis thaliana, Nicotiana tabacum and Beta vulgaris were suitable for genetic transformation recipients for functional analysis of CqGAI gene. Above results provided an important reference for further study the function and heterologous expression of CqGAI gene. [ABSTRACT FROM AUTHOR]

Superoxide dismutase (SOD) is a key enzyme in the antioxidant system of plants and plays a critical role in protecting plants from various biological and abiotic stresses. In this paper, using bioinformatics methods, 12 SOD genes were identified in quinoa by sequence alignment based on Arabidopsis SOD, which localized in the nucleus, microsomes and mitochondria, and distributed heterogeneously on 11 different chromosomes, and the tertiary structure of their encoded proteins showed that Cu/Zn-SODs were homodimers with Fe-SODs and Mn-SODs were homotetramers.The intron/exon distribution patterns of the CqSOD genes were different, with the number of introns ranging from 4 to 7, and the conserved motifs differing significantly. The phylogenetic relationships showed that the SOD proteins could be divided into 3 subfamilies: Cu/Zn-SODs, Fe-SODs and Mn-SODs. In addition, all CqFe- SODs and CqMn-SODs promoter regions contained abscisic acid(ABA)-responsive cis-elements, and CqSOD12 interacted with 11 CqSOD proteins and 4 CqCAT proteins. Expression profiling showed that the 12 CqSOD genes were strongly responsive to both mixed saline-alkali and nitroprusside. Above results laid the foundation for research on the role and molecular mechanism of SOD genes in plant development and stress response. [ABSTRACT FROM AUTHOR]

[Objective] To explore the role of AtlDD4 gene of Arabidopsis thaliana IDD gene family in regulating plant growth and stress resistance, the plant with overexpressing AtlDD4 gene was constructed. [Method] Total RNA was extracted from Arabidopsis leaves and inverted into cDNA. CDS fragments were amplified by PCR using specific primers. A recombinant plasmid was obtained by linking this target fragment to the 35S promoter of cauliflower mosaic virus. And the recombinant plasmid was transformed into Agrobacterium to construct the 35S AtIDD4 CDS Agrobacterium expression vector. Those vectors were transferred into wild type plants (WT) of A. thaliana using inflorescence immersion. After harvest, the collected T0 generation seeds were sown on MS medium containing hygromycin for resistance screening until T3 generation plants, yielding AtIDD4-OE plants which were overexpressing AtlDD4 gene. [Result] The expression level of AtlDD4 gene in AtlDD4-OE line was significantly increased by the semi-quantitative and quantitative RT-PCR examination, approximately twice as the wildtype plants. Compared with the WT plants, the root length and fresh weight of AtIDD4-OE seedlings grown vertically for 14 days in MS medium were significantly lower. AtIDD4-OE lines grown in soil for 14 days, showed weak growth status, short plants and small rosette leaf area. [Conclusion] Overexpression of AtIDD4 gene can inhibit the growth of Arabidopsis plants which function as a negative regulator during plants growth and development. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

β-ionone（β-ionone, ΒI）is an important natural aroma volatile compound widely distributed in plants, which is an apocarotenoid produced by carotenoid cleavage dioxygenases cleaving β-carotene at the 9, 10 and the 9', 10' positions. As a representative of cyclized isoprene, β-ionone has various biological activities such as antibacterial, antiviral and antitumor. Therefore, studies on the biosynthesis and metabolic regulation of β-ionone in plants has gradually become a hot topic. This paper summarizes the progress of β-ionone bioactivity, biosynthesis and regulation in plants, focusing on the β-ionone biosynthesis pathway and its related enzymes, followed by the control of β-ionone biosynthesis by carotenoid cleavage dioxygenase genes at the molecular level, as well as transcription factors such as WRKY, NAC, ERF and MYB regulate the expressions of related structural genes and thus affect β-ionone biosynthesis. And the paper describes the effects of environmental factors such as temperature, light, water and soil salinity on β-ionone biosynthesis in plants. It also introduces the effects of genes involved in the biosynthetic pathway of β-ionone on the color and aroma of plant leaves, flowers and fruits, as well as the improvement of β-ionone biosynthesis in plants using gene overexpression, gene silencing and other genetic engineering techniques, and makes reasonable prospects for the problems to be solved in the research on β-ionone in plants, aiming to provide theoretical assistance for the deeper research on the biosynthesis and regulatory mechanism of β-ionone in plants, and provide a theoretical foundation for improving plant floral aroma substances and obtaining a high yield of natural β-ionone. [ABSTRACT FROM AUTHOR]

Plant height is an important agronomic trait which affects maize architecture and planting density. Breeding plants with dwarf/ semi-dwarf architectures could tolerate high-density planting and contribute to yield increase. However, most dwarf mutants have severely reduced yield and are difficult to be applied in breeding. Therefore, it is particularly important to explore the regulation mechanism of maize plant height and to find out the excellent allele variation in plant height genes. It can improve the plant-type of maize, increase the light use efficiency and enhance the tolerance of population to water and fertilizer. Here we reviewed the quantitative trait locus of plant height excavated so far. We also explained that the cloned genes related to plant height are mainly regulated by plant hormones, microtubule-associated proteins and flower factors. Then we summarized the application and limitations of brachytic2 gene in maize dwarf breeding research, and looked forward to the important value of dwarf favorable alleles and their molecular markers, as well as modern biotechnology in the creation of dwarf germplasm resources. These efforts will promote the research on the genetic mechanism of maize plant height, and provide reference for the molecular breeding of dwarf maize. [ABSTRACT FROM AUTHOR]

Tartary buckwheat［Fagopyrum tataricum（L.）Gaertn］is rich in flavonoid C-glycosides, and it has many health effects, such as anti-oxidation, anti-cancer and anti-inflammation. In this study, in order to explore the flavonoid C-glycosyltransferase gene in tartary buckwheat, a glycosyltransferase gene named FtUGT143 was cloned from tartary buckwheat by RT-PCR（reverse transcription-polymerase chain reaction）, and its bioinformatics, molecular docking, gene expression, correlation between gene expression and metabolites content were also analyzed. The results showed that the full-length CDS sequence of FtUGT143 was 678 bp, encoding a protein of 226 amino acids. The codon preference analysis indicated that FtUGT143 had the codon usage preference of dicotyledons. Multiple sequence alignment and phylogenetic tree analysis suggested that FtUGT143 belonged to the carbon glycosyltransferase subfamily of plant glycosyltransferase gene family. The molecular docking analysis implied that FtUGT143 interacted with apigenin and luteolin, which were the substrates of flavonoid C-glycosides（vitexin, isovitexin, orientin and isoorientin）. RT-qPCR results showed that FtUGT143 gene was expressed in all tissues of tartary buckwheat, but it was significantly expressed in the roots, stems and leaves at seedling stage, and its expression in different tissues had a good correlation with the accumulation of four flavonoid glycosides. The research results showed that FtUGT143 is a C-glycosyltransferase which may participate in the biosynthesis of flavonoid C-glycosides, revealing the important role of FtUGT143 in the synthesis mechanism of flavonoid C-glycosides in tartary buckwheat. [ABSTRACT FROM AUTHOR]

Virus-induced gene silencing（VIGS）technology has been widely used in plant gene function research. The efficiency of gene silencing in soybean mediated by tobacco rattle virus（TRV）-based vector system remains to be clarified. The seamless cloning technique was used to construct a TRV-VIGS system to explore the silencing efficiency in soybean target genes in different tissues with different inoculation methods, providing a basis for gene function studies in soybean. Taken phytoene desaturase（GmPDS）and ubiquitin ligase（GmATL3）as target genes, pTRV1 and the recombinant vectors solution were inoculated into soybean（Glycine max）plants Zhonghuang13 by three methods: injection, agroinoculation and a combination of injection plus agroinoculation. The silencing phenotypes were observed at 28 d after inoculation, and the relative expressions of the genes in the roots and leaves were quantified by RT-qPCR to determine the silencing effectiveness of the different methods. Silencing of GmPDS resulted in yellowing and chlorosis on the edges and inside of the leaves, while chlorosis spots and fold chlorosis phenotypes in the leaves surface occurred. The results of RT-qPCR showed that silencing efficiency of GmPDS of all three inoculation methods was close to 100%. The silencing efficiency of GmATL3 by the injection was 80%-95% in the leaves and 40%-60% in the roots; the silencing efficiency of the root inoculation and injection plus root inoculation was 70%-90% in roots and 15%-50% in leaves. Different inoculation methods caused different levels of silencing phenotypes and silencing efficiencies for different endogenous genes. The silencing efficiency of an inoculation method on different tissues was different, and the highest silencing efficiency on the leaves and roots were injection method and injection plus agroinoculation, respectively. [ABSTRACT FROM AUTHOR]

Plant height and its relative traits are key factors that affect plant type and yield of sorghum. Mapping major QTL for these traits may provide a basis for genetic improvement in sorghum. In this study, a RI population including 205 lines obtained from a cross between American cultivar BTx623 and Guizhou liquor-brewing cultivar Hongyingzi was used to investigate plant height and internode numbers under five environments. The genetic map was constructed based on genotype data from Super-GBS technology, and major QTLs were identified by inclusive composite interval mapping（ICIM）. Totally, eighteen major QTLs were detected on chromosome 1, 3, 4, 8, and 9, of which 7 and 11 QTLs were related to plant height and internode numbers, respectively. Ten major QTLs were repeatedly detected in multiple environments or two traits, of which qPH9.1 was consistent with the known plant high gene Dw1, while qPH1.2, qPH3.2, qIN3.2 and qIN8.1 were colocalized with four homologous genes involving in plant height or internode elongation in rice. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Guangxi Normal University - Natural Science Edition is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To clarify the differences in the codon usage bias of δ8-FAD2 gene and the influencing factors between herbal oil crops, woody oil crops, and non-oil crops, provide the research basis for further improving the quality of oil crops through gene regulation, in this study, the complete gene sequences of δ8-FAD2 gene of 15 different types of plants were selected from GenBank database and their codon usage bias was analyzed. CodonW software was used to analyze the sequences to obtain important parameters such as A3, T3, C3, G3, GC3, effective codon number(ENC), and relative synonymous codon usage (RSCU). Correlation index analysis, optimal codon determination of multiple reference systems, ENC-plot analysis, PR2-plot analysis, and systematic relationship analysis were used to compare the gene sequences of 15 different plants. The results showed that the δ8-FAD2 codon usage bias of these 15 plant species was weak, and they preferred ending with A or T; the δ8-FAD2 gene had 17 dominant codons and 21 optimal codons; the optimal codon of δ8-FAD2 gene in different plants was different. According to the analysis of ENC-plot and PR2-plot, the codon bias of δ8-FAD2 gene in oil crops was stronger than that in non-oil crops, and the codon bias pressure was similar among woody plants, but different among herbal plants. Cluster analysis based on RSCU value and complete gene sequence showed that the genetic relationship between species was not the decisive factor for codon usage bias. [ABSTRACT FROM AUTHOR]

Plant viruses have a great adverse impact on vegetable production. Non-pharmaceutical measures are mainly adopted for vegetable virus prevention and control at present. However, the efficacy of broad-spectrum antiviral agents is limited. The new viral inhibitors are expected to be developed based on novel drug targets. G-quadruplex (G4s) is a non-canonical nucleic acid structure. The replication, transcription, and translation of genes can be regulated by the formation or unfolding of G-quadruplexes in the genome, which affects virus proliferation. Small molecules mediating the stability of G-quadruplex structures exhibit antiviral activity, making G-quadruplexes to be potential antiviral targets. As a widely distributed plant virus, tomato bushy stunt virus (TBSV) is considered as a model virus to study the interaction between the virus and host. The molecular mechanisms of its genome replication, transcription, and translation have been widely studied. Revealing the structure and function of G-quadruplex in TBSV is expected to lay a foundation for the research of G-quadruplex in plant virus genes. In this work, two conserved potential G-quadruplex sequences (putative G-quadruplex sequences, PQS) TBSV-PQS2 and TBSV-PQS4 were identified in TBSV genes through bioinformatics analysis. The G4 ligands interacting with TBSV-PQS2 and TBSV-PQS4 were screened out by UV fluorescence spectrum and circular dichroism (CD) spectrum. Invasive cloning experiments in tobacco found that G-quadruplex ligand N-methyl mesophorphyrin IX (NMM) inhibits TBSV virus proliferation. This work shows that the development of ecological pesticides against plant viruses by targeting the G-quadruplex in plant viruses is expected to become a new strategy for controlling the damage of plant viruses. [ABSTRACT FROM AUTHOR]

To study the difference of cold resistance of four plants of Rosa(R. lasiosepala, R. odorata. var. gigantea, R. weisiensis, R. lichiangensis), and screen out the varieties with strong cold resistance. In this study, a low temperature incubator was used to conduct different gradients of low temperature stress(0, -5, -10, -15 ℃), the soluble sugar content, soluble protein content, malondialdehyde(MDA) content, proline(Pro) content, SOD activity, POD activity, and expression difference of coldresistance-related regulatory gene HSP90 were measured. The results showed that under low temperature stress, the soluble protein content, soluble sugar content, free proline content, malondialdehyde content, SOD activity, and POD activity in the leaves of four plants of Rosa showed a gradually increasing trend with aggravation of low temperature stress. The analysis of the differential expression of HSP90 gene of four plants of Rosa showed that the relative expression of HSP90 gene showed a trend of gradual increase with aggravation of low temperature stress. The expression of HSP90 gene was the highest in R. lasiosepala under 0, - 5, - 10, -15 ℃ low temperature stress, indicating that there were differences in cold resistance of four plants of Rosa, among which R. lasiosepala had better cold resistance. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Late embryogenesis abundant (LEA) is widely present in organisms and closely related to plant resistence, it can protect plant cells and reduce plant damage under drought stress. Selaginella pulvinata is a fern with the ability to survive drought stress, with a strong recovery ability under drought stress. To investigate the molecular mechanisms and expression characteristics of the SpLEA1 gene in drought-tolerant plants, we used the highly drought-tolerant plant S. pulvinata as experimental material and obtained the cDNA sequence of the SpLEA1 gene by RT-PCR based on the transcriptome sequencing results. The promoter sequence was obtained by the HiTail-PCR technique, and the sequence was analyzed by bioinformatics. qRT-PCR was used to analyze the expression pattern of the SpLEA1 gene under drought stress. The results were as follows: (1) The length of SpLEA1 was 476 bp, the open reading frame (ORF) was 279 bp, and it encoded 92 amino acids. The predicted molecular weight of the protein was 9 491.46 Da, and the isoelectric point was 5.45. The predicted protein structure analysis showed that the protein was hydrophilic. The protein contained ten phosphorylation sites, of which six serines, three tyrosines, and one threonine, respectively, and the predicted secondary structures showed that the protein was mainly composed of α-helix and random coil. (2) The conserved structural domain of the SpLEA1 protein was predicted to be Lea-5, derived from the LEA1 family. Based on the phylogenetic tree and genetic distanced matrix, the SpLEA1 was found to have high homology with Lea-5 protein from Cicer arietinum and Trifolium pratense. (3) Predictive analysis of cis-acting elements in promoter sequenced revealed that the SpLEA1 gene promoter contained five classes of hormone response elements and functional elements related to the drought stress response. The SpLEA1 gene was hypothesized to have multiple functions in the plant body and was closely related to drought stress response mechanisms. (4) SpLEA1 gene expression was up-regulated under natural dehydration treatment and peaked in 12 h. After rehydration treatment for 24 h, expression was significantly down-regulated. In summary, the SpLEA1 gene is likely to be involved in the regulation of drought stress response mechanisms in matted curly cypress. This results provide the reference for further studies on the function of the matted cypress SpLEA1 gene under drought stress and its expression regulation mechanism. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives]The aim of this study was to identify members of the growth regulatory factor(GRF)gene family of Prunus mume, and systematically analyze their basic properties, evolutionary relationship and differential expression at different dormancy periods, in order to lay a theoretical foundation for further study of GRF function in P.mume. [Methods]The PmGRF gene family of P.mume was analyzed with the bioinformatics methods, to determine the gene structure, conserved motif analysis, physical and chemical properties of protein, chromosome localization, collinearity analysis, cis-acting elements and evolutionary relationship. The expression patterns of PmGRF gene family at different dormancy stages in P.mume were analyzed by RT-qPCR. [Results]The PmGRF family of P.mume contained 15 members, and the PmGRF gene was unevenly distributed on 6 chromosomes in P.mume. Most of the GRF proteins contained many basic amino acids, and all GRF proteins were hydrophilic proteins. Phylogenetic analysis showed that the GRF families of P.mume, apricot, almond and Arabidopsis thaliana were divided into seven subfamilies, and the homology between P.mume and apricot GRF families was high. The PmGRF promoter of P.mume was rich in elements related to growth and development. The collinearity map reflected that there were segmental repeats in the three pairs of genes PmGRF01 and PmGRF02, PmGRF09 and PmGRF11, PmGRF04 and PmGRF14. There was a linear relationship between AtGRF04 and PmGRF13, AtGRF05 and PmGRF04, indicating that they had homology. The Ka/Ks values of PmGRF gene repeats were far less than 1, indicating that the evolution process was subject to purification selection. PmGRF12, PmGRF06 and PmGRF09 had the highest transcriptional abundance in buds. RT-qPCR analysis showed that there were significant changes in the expression of PmGRF in most P.mume during the four dormancy periods. [Conclusions]PmGRF basically conforms to the typical characteristics of plant GRF gene. According to the analysis of the structure and expression, it can be hypothesized that PmGRF12, PmGRF06 and PmGRF09 may be involved in the dormancy process of buds. [ABSTRACT FROM AUTHOR]

Oryza sativa formin homology 1(OsFH1)plays a crucial role in rice root-hair growth and development, and the short root-hair phenotype of osfh1 was changed by environmental factors. However, the mechanism of how environmental factors interact with OsFH1 to regulate rice root-hair phenotype is still unknown. To determine whether OsFHs function in the process of osfh1 mutant root-hair phenotype recovery. The expression of OsFHs was analyzed by qRT-PCR in the osfh1 mutant treated under 1/2 MS liquid culture and 1/2 MS solid culture. Furthermore, qRT-PCR results were compared with bioinformatics analysis results. The results were as follows:(1)When compared with the wild type, osfh1 primary root showed a no root-hair. The osfh1 mutant showed a shorter shoot and more lateral roots. However, the no root-hair phenotype of osfh1 recovered under 1/2 MS solid culture treatment.(2)The expression of OsFH16 decreased and the expression of OsFH17 increased in the osfh1 mutant from liquid culture treatment to solid culture treatment with signifcant differences.(3)OsFH1, OsFH16 and OsFH17 all belonged to the Type Ⅱ subfamily, and all had cis-acting elements related to environmental stress, such as auxin, gibberellin, and anaerobic. OsFH1, OsFH16, and OsFH17 may be located in the plasma membrane to perform functions.(4)Analysis of the tissue-specific expression pattern of OsFHs showed that OsFH1 was highly expressed in the roots, while OsFH16 and OsFH17 were lowly expressed in the roots. In conclusion, this study suggests that OsFH1, OsFH16, OsFH17 have conservative structures and similar regulatory modes, and all three may function on the cell plasma membrane, therefore, OsFH16, OsFH17 may be involved in the process that environmental factors and osfh1 together alter the root-hair phenotype. Overall, this study lays a theoretical foundation for the mechanism research of environmental factors and osfh1 gene co-regulation of rice root-hair development and proposes a new direction for exploring the function of plant formin genes. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The present paper aimed to study the function of GhUGT76 C1 gene in regulating the hight of upland cotton. 【Method】GhUGT76 C1 gene was cloned for bioinformatics and evolutionary analysis using upland cotton dwarf mutant LA-1 as material. Overexpression vector of GhUGT76 C1 were constructed and the overexpression transformed Arabidopsis plants were created using the transformation method mediated by Agrobacterium infection. The function of GhUGT76 C1 in regulating the dwarfnism of LA-1 were analyzed through phenotypic identification of transgenic lines under different light quality and intensity treatment. 【Result】GhUGT76 C1 gene was cloned successfully. Bioinformatics analysis showed that the gene was 1447 bp in length and the CDS region was 1365 bp which encoded 454 amino acids, the theoretical relative molecular mass of the gene was 51. 31 kD, the theoretical soelectric point was 5. 40 and the protein was a unstable protein. In the secondary structure, there were 10 alpha helix regions, 17 beta lamella regions, 22 beta turn regions and 22 random coil regions in total. Phylogenetic tree analysis showed that this gene was most closely related to Arabidopsis AtUGT76 C1 gene. We successfully constructed GhUGT76 C1 gene overexpressed vector and got Arabidopsis overexpressed mutant through transformation. We analysed the phenotypic of GhUGT76 C1 gene overexpressed mutants under different light quality and intensity, the results showed that the mutants had significantly increased hypocotyl growth phenotype under dark, low light intensity blue and far red light conditions. 【Conclusion】This study laid a foundation for the analysis of the function of GhUGT76 C1 gene in regulating plant height and the molecular mechanism of LA-1 dwarfism, and provided research materials for the in-depth understanding of the regulation mechanism of height in Gossypium hirsutum. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Histone deacetylases（HDACs） can actively respond to a variety of abiotic stresses. To understand whether the HDACs genes of mangrove plants Kandelia candel and Aegiceras corniculatum are involved in abiotic stress responses, whole-genome sequencing and evolutionary analysis of the HDACs of K. candel and Aegiceras corniculatum were conducted. As a result, 13 and 11 HDAC family members were identified in K. candel and Aegiceras corniculatum, respectively; these genes were divided into three subfamilies: RPD3/HDA1, SIR2, and HD2, and each subfamily had a similar gene structure and conserved domain. Chromosome mapping showed that the evolution of HDACs in K. candel and Aegiceras corniculatum occurred mainly through purification selection. Promoter analysis showed that the HDACs of the two species had many types of cis-acting elements among which hormones and light response-related elements were the primary ones. In addition, the HD2 subfamily with irregular curl accounting for 65% found that the N-terminal of the sequence lacks a conserved region of the pentapeptide motif（MEFWG） and is mostly surrounded by acidic or basic amino acids. The results of expression analysis showed that KcHDT2 and AcoHDT1 genes closely clustered with the HD2 subfamily of Arabidopsis thaliana and were highly expressed in the roots and fruits. HD2 genes were mostly involved in plant regulation of salt tolerance, hypoxia, and strong light stress. Our comprehensive analysis showed that the HDAC family members of K. candel and Aegiceras corniculatum are both conservative and specific in structure and function and that the HD2 subfamily members have potential functions in response to abiotic stress. [ABSTRACT FROM AUTHOR]

BACKGROUND: Edible vaccines from transgenic plants is to integrate exogenous genes into the plant genome with plants as carriers, further activating animal or human immune system to obtain specific immunity. However, the continuous low expression of exogenous genes has been unable to achieve satisfactory immune effects. OBJECTIVE: To detect the foreign fused gene pacA-ctxB gene and interest protein expression level in the transgenic tomato by molecular biological technique, providing a research basis for further observation of the anti-caries effect of edible caries vaccines. METHODS: After extracting the total DNA of transgenic tomato leaves, the exogenous fused gene pacA-ctxB was detected by PCR. There were three groups in the experiment: a positive control group (plasmid p2355-EPC10), a blank control group (an ordinary tomato plant, Hongkang 219), and a transgenic group (nine transgenic tomato plants). Total proteins were extracted and quantitatively tested with BCA kit and expression of PAcA/CTB in the transgenic tomato was analyzed by western blot and ELISA. There were two groups for detection of target proteins: a transgenic group (five transgenic tomato plants expressing exogenous chimeric genes (positive PCR test) and a blank control group (one ordinary tomato plant). RESULTS AND CONCLUSION: PCR amplification results showed that there were 5 of the 9 strains of transgenic tomatoes in which the specific amplification bands were about 1.7 kb, accounting for 55% of the total detected transgenic plants. The total protein in the transgenic tomato was 3.15 g/L. Western blot results showed high density bands at 5.8 kD for PCR-positive transgenic tomato protein samples, and no specific bands were found in non-transgenic tomato protein samples. ELISA results showed that the expression of target proteins was about 4.12 mg/L, accounting for 0.13% of the total soluble protein in the transgenic tomato. To conclude, the exogenous fusion gene pacA-ctxB can be expressed in tomato plants and produce target proteins. [ABSTRACT FROM AUTHOR]

[Objectives]This article aimed to identify the trehalose-6-phosphate phosphatase(TPP)family genes of tea plant(Camellia sinensis), and explore their functions in the regulation of tea plants against low temperature stress. [Methods]The evolutionary relationship of the genes, the conserved domains of the coding protein, and the cis-elements of the promoter were analyzed by bioinformatics methods. Then, the expression of CsTPP in different tissues, and the response to low temperature stress were explored. [Results]Twelve CsTPP sequences with Trehalose-PPase conserved domains were identified from the tea plant genome. The phylogenetic analysis showed that CsTPP was divided into three groups according to their homology differences with TPP genes of Arabidopsis and Oryza sativa. The promoter analysis showed that CsTPP contained multiple stress response related and hormone response elements. Gene expression analysis showed that the expression levels of 12 CsTPP genes varied greatly in different tissues and had marked with tissue expression specificity. The analysis of response to cold stress showed that four CsTPP genes might be involved in tea leaves in response to cold stress process. [Conclusions]The CsTPP gene family might participate in low temperature stress of tea plant. [ABSTRACT FROM AUTHOR]

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Plant lysin motif receptor-like kinase(LYKs)are an important type of RLK found in plants. They have a non-negligible role in plant growth and development, resistance to adversity stress, etc. It is a gene function in plants research hotspots. In order to better understand the LYK gene in Physcomitrella patens, we identified and analyzed members of the LysM-type receptor kinase gene family of P. patens, using bioinformatics and preliminarily discussed the relationship between LYK gene structure, evolution and function by analyzing the basic physical information, gene structure, chromosomal location and phylogenetic relationship of the LYK family members. The results were as follows:(1)There were 21 LYK genes in the moss, the amino acid sequence size ranged from 625 to 755 aa, the molecular weight ranges from 69.54 to 82.02 kDa, and the isoelectric point ranged from 5.98 to 7.78.(2)A phylogenetic tree was constructed by combining all the LYK proteins of P. patens with the LYK proteins of three typical model plants(Rice, Arabidopsis, and Medicago truncatula). All LYK proteins were divided into four subgroups(LYK-I, LYK- II, LYR-I and LYR-II). The genetic structure and conserved domain characteristics of the members in each subgroup of P. patens showed relatively similar characteristics, and it was speculated that they might have the same or similar functions.(3)Chromosome mapping found that 21 LYK genes were concentrated on four chromosomes and there were small gene clusters, which may also be related to gene function. This article analyzed the basic information of the LysM receptor-like protein kinase gene family of P. patens, which can lay the foundation for the in-depth study of the physiological and biochemical functions of its LYK gene family members. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] This study aimed to explore the relationship of ANW16F plant heights and its components and partial yield traits and estimate the main gene + polygene quantitative genetic mode of plant heights and its components, which would provide a reference for breeding utilization of Rht16 and locating the major QTL by using molecular markers. Method ] ANW16F ( Triticum durum, 2n = 4X = 28, AABB), LD222 ( high stalk control) ( Triticum durum, 2n =4X =28, AABB) and its F1, F2, generation plants were used as tested materials. The genetic traits of plant heights and its components, partial yield traits were analyzed. (Result) The ANW16F reduced the plant height of 33.9% . The peduncle was the most important factor of plant height, plant height was extremely positively correlated with each stem node, and the highest correlation was found with the second internode from the top, followed by peduncle. Plant height and spike length were significantly positively correlated with spikelet number. Plant height fit into additive-dominant-epigenetic genetic mode (B-1 model) controlled by two major genes and additive effect was the main one. The heritability of major genes of plant height was 86. 07 %, which was less affected by the environment. The peduncle conformed to two pairs of additive-dominant major gene genetic models (B-2 model). The second, third and fourth internode from the top fit into a pair of additive-dominant major gene genetic models ( A-1 model). [Conclusion] Considering the proportion of plant height composition, correlation analysis and heritability, the peduncle and inverted 2 node can be used as important breeding indexes in dwarfing and high yield breeding with Rhtl 6 gene in the future and can be selected in the early stage. [ABSTRACT FROM AUTHOR]

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Phosphorus is the key nutrient factor for limiting the plant growth and production in subtropical evergreen broad-leaved forest. lt is of great significance to study on the molecular mechanism of dominant species response to low phosphorus stress in the forest community. Castanopsis chinensis is the dominant species in 20 hm² lower subtropical evergreen broad-leaved forest plot in Dinghushan and plays very important role in forest community assembly, and maintenance of biodiversity. ln this study, high-throughput sequencing technology combine d with bioinformatics software's were used to deep analyze the genome sequence of C. chinernis. Sequencing quality was assessed by Fast QC and clean data was demultiplexed by Slacks. The assembly of reference sequence of C. chinensis genom was preliminarily completed by dDocenl and the fasla formal file of genomic reference sequence totally contained 1 488 conlig sequences including forward strand and reverse strand. Sequencing alignment was conducted by llowlie2 and the individual sequence information was completely aligned lo reference sequence. the results were. as follows: ( 1) Pearnon condition analysis was performed using the data of soil available phosphorus content in Dinghushan plot with the result of sequence alignment matrix and the result showed that 37 significant phosphorus-responsive genes were detected. the GO function annotation analysis of the phosphorus-responsive genes found that among the 29 GO annotation classifications, there were 13 genes in the molecular functional class and the predicting functions included NAPE-specific phospholipase D activity, cytochrome-c oxidase activity, electron transporter and peroxidase activity. ( 2) The predicting function of 11 genes in the class of biological process class involved in HNA splicing, oxidative phosphorylation, photosynthetic electron transport in photosystem II. Cellular component contained nine genes, including chloroplast, integral component of membrane and photosystem II. Moreover, the result of KEGG analysis showed that the KEGG terns of 37 genes were significantly enriched lo the metallic pathway of photosynthesis and psbD was the major gene participated in the regulatory process. This study reveals that many phosphorus-responsive genes are involved in the regulation of various biological pathways in the growth stage of C. chinensis under low phosphorus stress psbD gene is a major phosphorus-responsive gene adjusting plant growth by regulating photosynthesis in leaves. The specific function of psbD gene in C. chinernis needs lo be fur1her verified. [ABSTRACT FROM AUTHOR]

In order to reveal the effects of Cd stress on the growth of B. napus seedlings, phenotype, physiological responses, and gene expression of B. napus under different concentration of Cd treatment were investigated through pot experiment. Our results showed that B. napus seedlings had a certain degree of tolerance to low concentration of Cd treatment since no significant difference in phenotype was observed between plants under 5 mg/kg and control. However, higher concentration of Cd treatment (30 and 50 mg/kg) could suppress the growth of B. napus seedlings, as the plant height, fresh weight, leaf area and chlorophyll content of B. nupus under higher concentration of Cd decreased significantly as compared with control, while soluble protein content and activities of two antioxidant enzymes (SOD and POD) increased significantly. The Cd content in both roots and leaves of oilseed rape increased sharply with the increased of Cd concentration. Meanwhile, Cd treatment also affected the accumulation of other metals in plants, such as Mn, Mg, Zn and Cu. Comparative transcriptome analysis of B. napus under different concentration of Cd treatments found that these differentially expressed genes were mainly concentrated in the anti-oxidant activity, photosystem, ATPase activity, cell wall organization or biogenesis and so on. Analysis of the expression patterns of HMA gene family showed that HMA3 was up-regulated, HMA2 and HMA4 were down regulated in roots of B. napus under Cd treatments when compared with control. These differentially expressed genes might have contributed to Cd tolerance of B. napus. [ABSTRACT FROM AUTHOR]

HAVOLUTA(PHV)transcription factor is one of the most important members of homeodomain-leucine zipper III(HD-ZIP) family, and plays an important role in the regulation of formation of plant stems apical and germ meristem. In this research, the PHV gene of Fraxinus mandshurica was acquired by gene cloning and was named by FmPHV. Detailed bioinformatics analysis showed that the length of FmPHV gene was 2 112 bp, and contained a complete Open Reading Frame(ORF)which encoded a protein with 703 amino acids. The FmPHV gene encoded a stable hydrophilic protein, the subcellular localization prediction of FmPHV protein showed the FmPHV was mainly present in the chloroplast. Conserved domain and homology analysis of FmPHV protein indicated that, the homology of FmPHV between the homology PHV proteins in other species, such as Olea europaean, Sesamum indicum and Nicotiana tabacum, was 99%. Under 4 °C condition, with the treatment of 50 mg·L-1 3-indolebutyric acid(IBA)solution, we obtained the cambium callus from cambium stem cells of Fraxinus mandshurica. Analysis of the gene expression pattern of FmPHV showed that the FmPHV was the highest expression in June. Furthermore, the expression of FmPHV in buds was stronger than which in any other tissues. Compared with the callus from different sources, which obtained from the bark of F. mandshurica, the expression level of FmPHV gene in callus, derived from meristem formation, was significantly higher than which from other sources. Moreover, overexpression the FmPHV gene in F. mandshurica seedlings will reduce the expression of auxin-related genes and increase the expression of cytokinin-related genes. This phenomenon indicated that the function of FmPHV gene was more beneficial to the process of plant shoot differentiation and regeneration. In summary, this research revealed the expression pattern of FmPHV gene during the growth and development in F. mandshurica. And revealed the expression pattern of key genes in plant shoot regeneration pathway, which were regulated by FmPHV gene. Our research reveals the potential value of the molecular characteristic of FmPHV gene in the response of auxin and cytokinin pathways during the process of the formation of plant callus generation. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

TIR-NBS gene is a class of gene closely related to plant disease resistance regulation and growth and development, and its characteristic analysis contributes to the understanding of plant immunity and developmental regulation. Through bioinformatics analysis method, the TIR-NBS gene family of tobacco was identified and molecularly analyzed from the aspects of gene identification, chromosome distribution, gene structure, protein properties and structure, signal peptide, subcellular localization and cis-acting elements. The results were as follows: The TIR-NBS gene family contained 30 members, distributed on 21 chromosomes, containing 3-8 conserved motifs; The encoded proteins were unstable proteins, no signal peptides, mainly distributed in the nucleus, cytoplasm and chloroplast; The secondary structure was characterized by α-helix and random coiling; It was mainly selected by purification in evolution, and had the closest relationship with TIR-NBS gene of tomato; Analysis of cis-acting elements showed that it may be regulated by light, temperature, and hormones such as auxin, methyl jasmonate, abscisic acid, salicylic acid, and gibberellin. The results provided a basis for further exploring the biological functions of the TIR-NBS gene and improving plant yield and quality. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】Using the exogenous GlgC gene may increase rapeseed starch accumulation, which can provide a potential new way for rapeseed breeding with high oil content. 【Method】The GlgC gene constructed two plant expression vector named pMB-DOF-TG and pMB-Cruc-TG, which was introduced into Agrobacterium tumefaciensLBA4404 and expressed itself into Brassica napus plants. 【Result】The results indicated that the construction of the GlgC gene plant expression vector was successful and useful, which was proved by moleculer detection. 【Conclusion】Positive transgenic GlgC lines were obtained, which laid a foundation for the application of genetic improvement technology in rapeseed breeding. [ABSTRACT FROM AUTHOR]