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For visible and Synthetic Aperture Radar (SAR) image fusion, this paper proposes a visible and SAR image fusion algorithm based on a Transformer and a Convolutional Neural Network (CNN). Firstly, in this paper, the Restormer Block is used to extract cross-modal shallow features. Then, we introduce an improved Transformer–CNN Feature Extractor (TCFE) with a two-branch residual structure. This includes a Transformer branch that introduces the Lite Transformer (LT) and DropKey for extracting global features and a CNN branch that introduces the Convolutional Block Attention Module (CBAM) for extracting local features. Finally, the fused image is output based on global features extracted by the Transformer branch and local features extracted by the CNN branch. The experiments show that the algorithm proposed in this paper can effectively achieve the extraction and fusion of global and local features of visible and SAR images, so that high-quality visible and SAR fusion images can be obtained. [ABSTRACT FROM AUTHOR]

A standardized preparation process is proposed in this study for achieving optimal strength and vegetative properties in vegetated concrete, using Yunnan red soil as a growth substrate for plants. The porosity of vegetated concrete is a crucial factor influencing plant growth, while compressive strength is a significant mechanical property. To assess the strength and porosity of vegetated concrete, different design porosities (22%, 24%, 26%, 28%) and cement-to-aggregate ratios (4, 5, 6, 7) were utilized in the preparation of vegetated concrete samples. The shell-making and static-pressure-molding methods were optimized for specimen preparation. Analyzing the stress–strain full curve characteristics of vegetation-type concrete under different influencing factors, an in-depth investigation into its failure mechanism was conducted. It was determined that the design porosity and cement content significantly impact the concrete's performance, particularly in terms of 30-day compressive strength and effective porosity. Furthermore, an increase in the fly ash ratio led to an increase in porosity and a decrease in compressive strength, providing a certain guidance for optimizing concrete performance. Comparative analysis through vegetation experiments revealed that black rye grass exhibited favorable growth adaptability compared to other grass species. [ABSTRACT FROM AUTHOR]

A digital civilization rising on silicon-based technology is predicted to replace carbon-based spaces with virtual ones, leading to the decline of rural regionalism. Reconciling the benefits of globalization through data-driven methods while preserving the locality of rural areas is crucial. The extensive collection of traditional villages in the Yunnan Province possesses abundant natural heritages and diverse regional cultural resources. However, the existing assessment and identification index system for these traditional villages primarily relies on qualitative measures, which lack a consistent and quantifiable data system that can provide scientific, data-driven analysis. This study focuses on the mainstream types of traditional villages in the Yunnan Province as its research subject. Based on empirical research methods and architectural typology principles, two data acquisition and vectorization procedures are implemented, combining tilt photography and digital mapping technology. This approach addresses the inconsistency problem of the current system's complex information and multiple data. This study aims to establish an efficient, objective, and consistent method of generating a database that comprehensively represents the environmental and architectural characteristics of the villages. Once established, this database could serve as a consistent and objective basis for subsequent quantitative analyses across different types of villages. Hence, setting up a consistent, quantifiable database is the first step to future data-driven policymaking, which is essential for sustainable preservation. In conclusion, the repeatability and reproducibility of these research findings are intrinsically tied to the meticulous methodology employed in the process of data acquisition and vectorization. The valid result was demonstrated after the verifying of the correlation analysis. Finally, a challenge to the universality came from adding different worldwide villages to the database and revealing underlying patterns. [ABSTRACT FROM AUTHOR]

To study the corrosion behavior of cement soil in peat soil, the experiment involves the preparation of peat soil by incorporating humic acid into cohesive soil with a lower organic matter content. Cement soil samples are then prepared by adding cement to the mixture. These samples are subjected to immersion in fulvic acid solution and deionized water to simulate different working environments of cement soil. The experiment considers immersion time as the variable factor. It conducts observations of apparent phenomena, ion leaching tests, and unconfined compression strength tests on the cement soil. The experiment results are as follows: (1) With increasing immersion time, the surface of the cement soil in the peat soil environment experiences the disappearance of Ca(OH)2 and calcium aluminate hydrate. Additionally, large amounts of bird dropping crystals precipitate on the surface and within the specimen. The cement soil undergoes localized disintegration due to extensive erosion caused by swelling forces. (2) In the peat soil environment, fulvic acid reacts with the hydration products of cement, resulting in partial leaching of ions such as Ca2+, Mg2+, Al3+, and Fe3+ into the immersion solution. The lower the pH of the fulvic acid immersion (indicating higher concentration), the more significant the ion leaching. Increasing the ratio of humic acid to cement can slow down the leaching of ions. The cement soil undergoes dissolutive erosion in the peat soil environment. (3) The peat soil environment exerts both strengthening and corrosive effects on the cement soil. Cement soil without humic acid exhibits noticeable corrosion in the peat soil environment, gradually decreasing strength as immersion time increases. The strength decreases by 83% from 28 to 365 days. In contrast, cement soil with humic acid experiences an initial period of strengthening, leading to a significant increase in strength in the short term (34% increase from 28 to 90 days). However, the corrosive effects gradually dominate, resulting in a decrease in strength over time. The strength decreases by 80% from 90 to 365 days. This study also explores the strengthening effects of peat soil on cement soil. It identifies phenomena such as extensive erosion and new substance precipitation in cement soil. [ABSTRACT FROM AUTHOR]

Many peat soils are distributed around plateau lakes, and the reinforcement of peat soils with high organic matter content by ordinary cement cannot meet the actual engineering requirements. In order to obtain better mechanical properties and durability of the reinforcement, this experiment prepared peat soil by mixing humic acid reagent into the alluvial clay soil with low organic matter content. The cement soil samples were prepared by adding cement and ultrafine cement (UFC) by stirring method; the samples were then soaked in fulvic acid solution to simulate the cement soil in the peat soil environment. Using the unconfined compressive strength (UCS) test, scanning electron microscope (SEM) test, and pores and cracks analysis system (PCAS) test, the effect of UFC content change on cement soil's humic acid erosion resistance was explored, and the optimal UFC content range was sought. The results of the UCS test show that with an increase in immersion time, the strength curves of cement soil samples gradually increase to the peak strength and then decrease. Significant differences in the time correspond to the peak strength, and the overall presentation is two processes: the strength enhancement stage and the corrosion stage of the sample. The incorporation of UFC makes the cement soil in the peat soil environment exhibit excellent corrosion resistance, and the optimal UFC content is 10%. The results of the SEM and PCAS tests show that the microstructure of cement soil after immersion time exceeds 90 days, increases with an increase in immersion time, and its structural connectivity gradually weakens. The excellent characteristics of UFC particles, such as small particle size, narrow particle size distribution, fast hydration reaction rate, high hydration degree, and many hydration products, weakened the adverse effects of humic acid on the cement soil structure to a certain extent. Therefore, although the number of macropores increases, they are not connected. It still presents a relatively compact honeycomb overall structure, which correlates well with the UCS results. [ABSTRACT FROM AUTHOR]

Replacing conventional aggregate with steel slag waste can boost the microwave absorption properties of asphalt mixtures and reduce pollution to protect the environment. In order to achieve the best healing in steel slag asphalt mixture, the optimum particle size and content of steel slag are essential. For this purpose, a high-efficiency algorithm for the random growth and placement of convex polyhedron aggregate is proposed in this paper. The limestone aggregate is replaced with an equal volume of steel slag, and a three-dimensional mesoscale random model of steel slag asphalt mixture is developed. The process of microwave heating is simulated by FEM. The numerical simulations are compared with the reported experimental data, which proves that the model is reliable (R2 = 99.40%). Both the volume average temperature and the uniformity of temperature distribution indicate that the steel slag replacement rate of 60% at 4.75–9.5 mm and 60% at 9.5–13.2 mm is optimal, among which the heat transfer of 4.75–9.5 mm steel slag is more uniform, and the temperature gradient is lower. Steel slag can dramatically increase the heating rate of an asphalt mixture, and the peak of the temperature gradient is around the boundary of steel slag. The reflection properties of steel slag may be related to the dielectric constant, permeability, and particle size. Excess steel slag will cause overheating in most zones of the specimen and will also depress the absorption efficiency of microwaves. The coefficient of variance for spherical (0.36) and polyhedral (0.32) aggregate specimen temperatures indicates that the aggregate's shape has a negligible effect on the heat transfer of asphalt mixtures. [ABSTRACT FROM AUTHOR]

The influence of peat soil environment (PSE) on the mechanical properties of cement-soil in the area around Dianchi Lake and Erhai Lake in Yunnan Province has attracted much attention. This study explores the change law of cement-soil UCS in the PSE, and provides guidance for the development and sustained usage of peat soil foundation. The paper discusses the preparation of cement-soil samples by adding humic acid (HA) and cement to cohesive soil with low organic matter content (blending method) and soaking it in fulvic acid (FA) solution and deionized water (steeping method) to simulate the actual working environment of cement-soil. Unconfined compressive strength (UCS), acid consumption, ion leaching, scanning electron microscope (SEM), and X-ray diffraction (XRD) tests are carried out on cement-soil samples soaked for 90 days. The results show that HA can significantly reduce the UCS of cement-soil. FA can reduce the UCS of cement-soil when the content of HA is less than 18%. However, when the amount of HA is more than 18%, the UCS of cement-soil increases slightly. FA makes the deformation and failure type of cement-soil gradually change from brittle shear failure to plastic shear failure. FA reacts with the cement hydration products in the sample so that the cumulative acid consumption of the cement-soil sample continues to increase, and the dissolution of Ca2+, Mg2+, Al3+, and Fe3+ in the sample increases the ion concentration of the soaking solution. In addition, SEM and XRD show that HA can increase the macropores and connectivity of cement-soil, while FA fills part of the pores of the wetting layer. In the PSE, FA can strengthen the inner structure of HA particles and fill and cement the layers of cohesive particles, enhancing the construction of cement-soil with HA content greater than 18%, so that its UCS is relatively improved. However, when the amount of HA is less than 18%, there are more small pores in the cement-soil. The interaction between FA and HA in the cement-soil is weak. The influence of FA on cement-soil is mainly a weakening effect, and its UCS is relatively reduced. [ABSTRACT FROM AUTHOR]

Peat soil is widely distributed in the Dianchi Lake area of Yunnan, and the effect of the cement deep-mixing method on peat soil foundation is mainly affected by humic acid (HA). In this paper, a composite cement curing agent is formed by adding different proportions of ultra-fine cement (UFC) to ordinary Portland cement (OPC) and used to cure the HA-containing cohesive soil. Mercury intrusion porosimetry (MIP), scanning electron microscopy (SEM), and X-ray powder diffraction (XRD) are used to study the influence mechanism of UFC on the micropore structure of HA-containing cemented soil. The unconfined compressive strength test (UCS) is used to verify it. MIP, SEM, and XRD results show that UFC can significantly improve the microscopic pore structure of the samples. The hydration reaction rate of cement increases with the increase in the proportion of UFC, and the generated hydration products can fill the pores of the samples. The filling effect of hydration products on macropores is enhanced, and the pores change from fibrous filling to cemented filling. The enhanced cementation of the hydration products improved the loose and overhead structure inside the sample. Enhancing the cementation of hydration products improves the loose and overhead structure inside the sample and the integrity of cemented soil. UCS verified that the increase in the UFC proportion increases the HA-containing cemented soil strength. It achieves the purpose of reducing the amount of cement when curing peat soil foundations and supports reducing carbon emissions in practical projects. [ABSTRACT FROM AUTHOR]

The organic soil in the Dianchi Lake area contains much humic acid (HA), and the impact of HA on cemented soil has attracted much attention. In this study, test soil is prepared based on the actual HA content of the soil in Dianchi Lake, and cement is added to prepare samples. Unconfined compressive strength (UCS), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), and X-ray diffraction (XRD) tests are conducted on the samples. The strength development and microstructure changes of cemented organic soil are studied. The results show that (1) cemented soil strength decreases with the increase in HA content and increases with the cement mixing ratio; (2) SEM and EDS tests show that with the increase in HA content, the connection between soil particles gradually changed from surface–surface to point–surface, point–edge, or point–point connection. A large overhead structure is inside the cemented soil, and its integrity weakens with increased HA content. However, elements such as Ca, Si, and Al exist in the pores of cemented soil, indicating that cement hydration products are cemented between soil particles. XRD testing shows that with the increase in HA content, the intensity of the diffraction peaks of hydration products in cemented soil gradually decrease. (3) In general, HA will gradually deteriorate the integrity of cemented soil and reduce its strength. When the HA content is more than 15%, the effect of HA on strength is more prominent. However, increasing the cement mixing ratio can effectively weaken HA's negative effect on cemented soil's strength. When the cement mixing ratio is between 15% and 25%, the strength of cemented soil with high HA content can be effectively increased. However, this approach is not conducive to the protection of the environment. [ABSTRACT FROM AUTHOR]

The organic soil is widely distributed around Dianchi Lake in Kunming, which is rich in the humic group (HG). In order to explore the effect of HG on the strength development of organic soil, this paper adopts the method of adding humic acid (HA) reagent into the undisturbed cohesive soil (blending method) and soaking it in fulvic acid (FA) solution (steeping method) to simulate the organic soil. Then, the effect of HG on soil is analyzed by the unconfined compressive strength (UCS) and the scanning electron microscope (SEM) tests. The UCS test shows that HA can significantly reduce the UCS of the samples, and it continues to decrease with the increase of the HA reagent content. FA can substantially enhance the UCS of the samples, which continues to increase with the concentration of FA solution (decreasing the pH value). Under each HG condition, the steeping of FA can significantly increase the UCS of the sample when the content of the HA reagent is less than 10%, and the growth rate of the UCS decreases when it is greater than 10%. With the increase of steeping age, the UCS of the 5% HA samples continued to increase and gradually became stable, and when the content of the HA reagent is more than 5%, the UCS curve first increases and then decreases. The SEM test shows that adding HA reagent can significantly increase the pore size, improve the connectivity between the pores, and weaken the connection of the soil structure. The steeping of FA can reduce the pore size, weaken the connectivity between the pores, and strengthen the connection of the sample structure. [ABSTRACT FROM AUTHOR]

The disposal of soil containing humic acid (HA) poses an increasingly difficult problem for geotechnical environmental engineering. In this study, the unconfined compressive strength (UCS) test of cement-soil with different HA contents is conducted to analyze the effect of HA on the strength of cement-soil. Mercury intrusion porosimetry (MIP), scanning electron microscopy (SEM), and X-ray diffraction (XRD) experiments reveal the effective mechanism of HA on the strength of cement-soil from the microscopic level. The results show that the strength of cement-soil decreases gradually with the increase of HA content. The failure mode of cement-soil slightly changed from brittle failure to plastic failure. The microscopic test shows that the incorporation of HA will lead to the apparent enlargement of the pores in the cement-soil. The large pores in the sample increase, the small micropores decrease, and the structure tends to be loose and overhead. The increased HA content added will significantly reduce the cement hydration products and destroy the cementation of the hydration products. [ABSTRACT FROM AUTHOR]

Simple Summary: Toxoplasmosis is one of the most common parasitic zoonoses in the world. It not only threatens human health and safety but also causes considerable losses to the global animal husbandry industry. Swine, as an intermediate host of Toxoplasma gondii, has important economic and public health significance, and the global prevalence of T. gondii in swine varies between 10% and 60%. Therefore, it is important to establish simple, effective, sensitive and specific diagnostic methods for the detection and prevention of T. gondii infection in swine. In the present study, we developed a colloidal gold immunochromatographic strip based on rTgROP14, recombinant protein A and monoclonal antibody TgROP14-5D5 for serological detection of T. gondii in swine populations. This new ICT achieved good specificity and sensitivity and has potential for routine serological detection of T. gondii in swine. Toxoplasma gondii, a worldwide distributed apicomplexan protozoan, can infect almost all warm-blooded animals and may cause toxoplasmosis. In order to provide a point-of-care detection method for T. gondii infection, an immunochromatographic test (ICT) was established. The proposed test uses recombinant T. gondii rhoptry protein 14 (ROP14) conjugated with 20 nm gold particles, recombinant protein A as the detection line and monoclonal antibody TgROP14-5D5 as the control line. The specificity, sensitivity, positive predictive value, negative predictive value and stability of this new ICT were evaluated. rTgROP14 was specifically recognized by positive serum of T. gondii but not negative serum. mAb TgROP14-5D5 showed higher specific recognition of T. gondii antigens and was therefore selected for subsequent colloidal gold strip construction. The new ICT based on TgROP14 exhibited good diagnostic performance with high specificity (86.9%) and sensitivity (90.9%) using IHA as a "reference standard". Among 436 field porcine sera, ICT and IHA detected 134 (30.7%) and 99 (22.7%) positive samples, respectively. The relative agreement was 87.8%. These data indicate that this new ICT based on TgROP14 is a suitable candidate for routine testing of T. gondii in the field. [ABSTRACT FROM AUTHOR]

The disposal of peat soil poses an increasingly difficult problem for actual engineering projects of Dianchi Lake area. This study obtains peat soil from seven areas around Dianchi Lake, and the content of humic acid (HA) in peat soil is between 2.36% and 28.13%. Then, this study simulates the peat soil by adding HA into the cohesive soil and uses cement to solidify it. The effect of cement and HA on the strength development of samples is examined by the unconfined compressive strength (UCS) test. Additionally, the microstructures of typical mixes are analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), and mercury intrusion porosimetry (MIP). The results showed that HA will significantly reduce the UCS of cement soil. The SEM, XRD, and MIP confirmed that cement hydration reaction increases with cement ratio. In addition to the cementitious soil particles, hydration products gradually fill the pores of the soil and effectively reduce the number of large-size (6000∼40,000 nm) pores in cement soil, which makes the soil particle framework stronger. When the cement ratio increases from 15% to 25%, the diffraction peak of CAH and CSH increases faster. Combined with the results of the UCS test, it could be proved that cement ratio greater than 20% weakens the influence of HA on the strength development of cement soil sample. [ABSTRACT FROM AUTHOR]

The effect and feasibility of peat soil environment (PSE) simulation pose a difficult problem for geotechnical environmental engineering. In this study, the actual content of humic group (HG) in peat soil of Dianchi Lake is determined, and the method of adding humic acid (HA) reagent into cohesive soil and soaking it in fulvic acid (FA) solution is used to simulate PSE of Dianchi Lake. By comparing the HG content of test samples and natural peat soil, the effect and feasibility of simulation test are studied. And the effects of HG on microstructure and material composition of PSE are analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) tests. The test results show that the content of HG and its components of PSE in seven sites of Dianchi Lake are quite different. The simulation method used in this study can simulate the PSE with different HA and FA contents, but the simulation method of soaking samples in FA solution cannot reach the actual effect. The SEM test confirm that the pore size and pore connectivity will increase with the HA reagent. However, FA is wrapped on the skeleton of soil through adsorption and fills some pores, which reduces the pore diameter and weakens pore connectivity. The XRD test shows that both HA and FA can reduce the diffraction peak of main substances in the samples, but not including SiO2. The reason is that HG and cohesive soil particles undergo coordination exchange and ion exchange; free HG combines with cohesive soil particles and transforms into bound HG, forming an organic-inorganic complex PSE. [ABSTRACT FROM AUTHOR]

Toxoplasma gondii (T. gondii) is an obligate intracellular parasitic protozoan that can cause toxoplasmosis in humans and other endotherms. T. gondii can manipulate the host gene expression profile by interfering with miRNA expression, which is closely associated with the molecular mechanisms of T. gondii -induced brain injury. However, it is unclear how T. gondii manipulates the gene expression of central nervous system (CNS) cells through modulation of miRNA expression in vivo during acute and chronic infection. Therefore, high-throughput sequencing was used to investigate expression profiles of brain miRNAs at 10, 25, and 50 days post-infection (DPI) in pigs infected with the Chinese I genotype T. gondii strain in this study. Compared with the control group 87, 68, and 135 differentially expressed miRNAs (DEMs) were identified in the infected porcine brains at 10, 25, and 50 DPI, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that a large number significantly enriched GO terms and KEGG pathways were found, and were mostly associated with stimulus or immune response, signal transduction, cell death or apoptosis, metabolic processes, immune system or diseases, and cancers. miRNA–gene network analysis revealed that the crucial connecting nodes, including DEMs and their target genes, might have key roles in the interactions between porcine brain and T. gondii. These results suggest that the regulatory strategies of T. gondii are involved in the modulation of a variety of host cell signaling pathways and cellular processes, containing unfolded protein response (UPR), oxidative stress (OS), autophagy, apoptosis, tumorigenesis, and inflammatory responses, by interfering with the global miRNA expression profile of CNS cells, allowing parasites to persist in the host CNS cells and contribute to pathological damage of porcine brain. To our knowledge, this is the first report on miRNA expression profile in porcine brains during acute and chronic T. gondii infection in vivo. Our results provide new insights into the mechanisms underlying T. gondii -induced brain injury during different infection stages and novel targets for developing therapeutic agents against T. gondii. [ABSTRACT FROM AUTHOR]

Wolbachia are maternally transmitted intracellular bacteria that can naturally and artificially infect arthropods and nematodes. Recently, they were applied to control the spread of mosquito-borne pathogens by causing cytoplasmic incompatibility (CI) between germ cells of females and males. The ability of Wolbachia to induce CI is based on the prevalence and polymorphism of Wolbachia in natural populations of mosquitoes. In this study, we screened the natural infection level and diversity of Wolbachia in field-collected mosquitoes from 25 provinces of China based on partial sequence of Wolbachia surface protein (wsp) gene and multilocus sequence typing (MLST). Among the samples, 2489 mosquitoes were captured from 24 provinces between July and September, 2014 and the remaining 1025 mosquitoes were collected month-by-month in Yangzhou, Jiangsu province between September 2013 and August 2014. Our results showed that the presence of Wolbachia was observed in mosquitoes of Aedes albopictus (97.1%, 331/341), Armigeres subalbatus (95.8%, 481/502), Culex pipiens (87.0%, 1525/1752), Cx. tritaeniorhynchus (17.1%, 14/82), but not Anopheles sinensis (n = 88). Phylogenetic analysis indicated that high polymorphism of wsp and MLST loci was observed in Ae. albopictus mosquitoes, while no or low polymorphisms were in Ar. subalbatus and Cx. pipiens mosquitoes. A total of 12 unique mutations of deduced amino acid were identified in the wsp sequences obtained in this study, including four mutations in Wolbachia supergroup A and eight mutations in supergroup B. This study revealed the prevalence and polymorphism of Wolbachia in mosquitoes in large-scale regions of China and will provide some useful information when performing Wolbachia-based mosquito biocontrol strategies in China. Author summary: The mosquitoes Aedes albopictus, Anopheles sinensis, Armigeres subalbatus, Culex pipiens and Cx. tritaeniorhynchus are native to China and the major vectors in the transmission of arboviruses, protozoans and nematodes. Recently, an innovative biocontrol strategy has been developed and evaluated based on the ability of Wolbachia to induce cytoplasmic incompatibility (CI), as well as interfere with the infection and replication of pathogens. Since the ability to induce CI largely depends on the density and diversity of Wolbachia, we investigated and characterized the natural infection of Wolbachia in above-mentioned five species of field-collected mosquitoes in 25 provinces of China. The results showed that the positive rates of Wolbachia infection were high in mosquitoes of Ae. albopictus, Ar. subalbatus and Cx. pipiens in large-scale regions of China and low in Cx. tritaeniorhynchus in Guizhou province. Phylogenetic analysis based on Wolbachia surface protein (wsp) gene and five multilocus sequence typing (MLST) loci indicated the high polymorphism of Wolbachia in Ae. albopictus, and low polymorphisms in Ar. subalbatus and Cx. pipiens. This finding contributes to the understanding of the nationwide distribution of Wolbachia and the potential application of this biocontrol strategy in China. [ABSTRACT FROM AUTHOR]

Summary: The obligate intracellular pathogen Chlamydia trachomatis is the most common cause of sexually transmitted bacterial disease. It secretes a protease known as chlamydial protease/proteasome-like activity factor (CPAF) that degrades many host molecules and plays a major role in Chlamydia pathogenesis. Here, we show that mature CPAF is a homodimer of the catalytic domains, each of which comprises two distinct subunits. Dormancy of the CPAF zymogen is maintained by an internal inhibitory segment that binds the CPAF active site and blocks its homodimerization. CPAF activation is initiated by trans-autocatalytic cleavage, which induces homodimerization and conformational changes that assemble the catalytic triad. This assembly leads to two autocatalytic cleavages and removal of the inhibitory segment, enabling full CPAF activity. CPAF is covalently bound and inhibited by the proteasome inhibitor lactacystin. These results reveal the activation mechanism of the CPAF serine protease and suggest new opportunities for anti-Chlamydia drug development. [Copyright &y& Elsevier]

As ubiquitous, persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) have adverse impacts on human health. Phenanthrene (Phe) is one of the most abundant PAHs in the environment. However, the long-term effects of exposure to environmental level of Phe on the kidneys and the potential mechanisms are unclear. T helper (Th) cells, a subtype of CD4 + T cells that play a central role in the renal immune microenvironment. In this study, male mice were chronically exposed to 5, 50, and 500 ng/kg bw Phe every other day for total 210 days. Those results indicated that environmental Phe exposure caused kidney hypertrophy, injury and fibrosis in the mice. Chronic, long-term environmental level of Phe exposure did not significantly alter the innate immune response but induced adaptive immune response changes (Th1/Th2 related cytokines release), causing a type 1 immune response in the 5 ng/kg bw Phe group and a type 2 immune response in the high dose groups (50 and 500 ng/kg bw). This study provides novel insights into the roles of adaptive immune response in long-term PAH exposure-induced chronic kidney injury and fibrosis, which is beneficial for further understanding the potential health hazards of PAHs and providing new avenues for immune intervention strategies to alleviate PAHs toxicity. [Display omitted] • Environmental level of Phe chronic exposure causes kidney hypertrophy and fibrosis in mice. • Adaptive immune response regulates kidney hypertrophy and fibrosis. • Mitochondrial and ER dysfunction contributes to Phe-induced kidney hypertrophy and fibrosis. [ABSTRACT FROM AUTHOR]

The economically significant genus Prunus includes fruit and nut crops that have been domesticated for shared and specific agronomic traits; however, the genomic signals of convergent and divergent selection have not been elucidated. In this study, we aimed to detect genomic signatures of convergent and divergent selection by conducting comparative population genomic analyses of the apricot-peach-plum-mei (APPM) complex, utilizing a haplotype-resolved telomere-to-telomere (T2T) genome assembly and population resequencing data. The haplotype-resolved T2T reference genome for the plum cultivar was assembled through HiFi and Hi-C reads, resulting in two haplotypes 251.25 and 251.29 Mb in size, respectively. Comparative genomics reveals a chromosomal translocation of ~1.17 Mb in the apricot genomes compared with peach, plum, and mei. Notably, the translocation involves the D locus, significantly impacting titratable acidity (TA), pH, and sugar content. Population genetic analysis detected substantial gene flow between plum and apricot, with introgression regions enriched in post-embryonic development and pollen germination processes. Comparative population genetic analyses revealed convergent selection for stress tolerance, flower development, and fruit ripening, along with divergent selection shaping specific crop, such as somatic embryogenesis in plum, pollen germination in mei, and hormone regulation in peach. Notably, selective sweeps on chromosome 7 coincide with a chromosomal collinearity from the comparative genomics, impacting key fruit-softening genes such as PG , regulated by ERF and RMA1H1 . Overall, this study provides insights into the genetic diversity, evolutionary history, and domestication of the APPM complex, offering valuable implications for genetic studies and breeding programs of Prunus crops., Competing Interests: The authors declare that they have no competing interests., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.)

A standardized preparation process is proposed in this study for achieving optimal strength and vegetative properties in vegetated concrete, using Yunnan red soil as a growth substrate for plants. The porosity of vegetated concrete is a crucial factor influencing plant growth, while compressive strength is a significant mechanical property. To assess the strength and porosity of vegetated concrete, different design porosities (22%, 24%, 26%, 28%) and cement-to-aggregate ratios (4, 5, 6, 7) were utilized in the preparation of vegetated concrete samples. The shell-making and static-pressure-molding methods were optimized for specimen preparation. Analyzing the stress-strain full curve characteristics of vegetation-type concrete under different influencing factors, an in-depth investigation into its failure mechanism was conducted. It was determined that the design porosity and cement content significantly impact the concrete's performance, particularly in terms of 30-day compressive strength and effective porosity. Furthermore, an increase in the fly ash ratio led to an increase in porosity and a decrease in compressive strength, providing a certain guidance for optimizing concrete performance. Comparative analysis through vegetation experiments revealed that black rye grass exhibited favorable growth adaptability compared to other grass species.

Grapevine is one of the most economically important crops worldwide. However, the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres, limiting the accessibility of the repetitive sequences, the centromeric and telomeric regions, and the study of inheritance of important agronomic traits in these regions. Here, we assembled a telomere-to-telomere (T2T) gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads. The T2T reference genome (PN_T2T) is 69 Mb longer with 9018 more genes identified than the 12X.v0 version. We annotated 67% repetitive sequences, 19 centromeres and 36 telomeres, and incorporated gene annotations of previous versions into the PN_T2T assembly. We detected a total of 377 gene clusters, which showed associations with complex traits, such as aroma and disease resistance. Even though PN40024 derives from nine generations of selfing, we still found nine genomic hotspots of heterozygous sites associated with biological processes, such as the oxidation-reduction process and protein phosphorylation. The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs., Competing Interests: The authors declare no conflict of interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.)

Trehalose is a disaccharide molecule consisting of two molecules of glucose. Industrially, trehalose is derived from corn starch and utilized as a drug. This study aims to examine whether the integration of nanoparticle-encapsulated trehalose to the Ice-Free Cryopreservation (IFC) method for preserving heart valves has better cell viability, benefits to protect the extracellular matrix (ECM), and reduce immune response after storage. For the experiment to be carried out, we obtained materials, and the procedures were carried out in the following manner. The initial step was the preparation of hydroxyapatite nanoparticles, followed by precipitation to acquire Apatite colloidal suspensions. Animals were obtained, and their tissue isolation and grouping were done ethically. All samples were then divided into four groups, Control group, Conventional Frozen Cryopreservation (CFC) group, IFC group, and IFC + T (IFC with the addition of 0.2 M nanoparticle-encapsulated Trehalose) group. Histological analysis was carried out via H&E staining, ECM components were stained with Modified Weigert staining, and the Gomori Ammonia method was used to stain reticular fibers. Alamar Blue assay was utilized to assess cell viability. Hemocompatibility was evaluated, and samples were processed for immunohistochemistry (TNFα and IL-10). Hemocompatibility was quantified using Terminal Complement Complex (TCC) and Neutrophil elastase (NE) as an indicator. The results of the H&E staining revealed less formation of extracellular ice crystals and intracellular vacuoles in the IFC + T group compared with all other groups. The CFC group's cell viability showed better viability than the IFC group, but the highest viability was exhibited in the IFC + T group (70.96 ± 2.53, P < 0.0001, n = 6). In immunohistochemistry, TNFα levels were lowest in both IFC and IFC + T group, and IL-10 expression had significantly reduced in IFC and IFC + T group. The results suggested that the nanoparticle encapsulated trehalose did not show significant hemocompatibility issues on the cryopreserved heart valves., Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2020.)

Père David's deer ( Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1 -positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1 -positive strains and provide insight into the dissemination of AMR in nature reserve settings. Sixty-seven mcr-1 -positive Escherichia coli isolates from 97 fecal samples were identified by PCR and found resistant to colistin. The prevalence of β-lactam resistance was very high, and there were 64 mcr-1 -positive isolates containing β-lactamase genes. Transconjugants of 66 mcr-1 -positive isolates were acquired through conjugation experiments. PCR-based replicon typing (PBRT) showed that 44 strains harbored IncI2 mcr-1 -bearing plasmids, eight strains harbored IncX4 mcr-1 -carrying plasmids, and 14 strains harbored IncHI2 mcr-1 -positive plasmids. Notably, mcr-1 was located in the chromosome of LD27-1. Clonal dissemination and horizontal dissemination of mcr-1 by plasmids coexist. We first report the prevalence of plasmid-mediated mcr-1 in E. coli from Père David's deer in China. mcr-1 -bearing IncI2 plasmid was the most frequent plasmid type, and the first IncI2 plasmid harboring both bla CTX-M-132 and mcr-1 is characterized here. Our results support the implication of Père David's deer as a potential reservoir for MCR-1-producing E. coli IMPORTANCE The mcr-1 gene is widely reported around the world and has been identified on various plasmids with different replicon types. Resistance to the last-line antibiotic colistin mediated by mcr-1 still represents a threat to global public health. Père David's deer is a highly endangered species originating in China, and many deer are currently being raised in captivity for gradual reintroduction to the wild. If this species carries AMR bacteria, it will pose a potential threat to the environment. Therefore, research on the dissemination of mcr-1 -positive E. coli from Père David's deer is of great significance. This is the first study to investigate the microbiological and genomic surveillance of MCR-1-producing bacteria colonized among Père David's deer in China, and we uncovered a high prevalence of MCR-1-producing E. coli The importance of constant surveillance for AMR bacteria in nature reserve settings is emphasized., (Copyright © 2020 Lu et al.)

Background: Ribosome display technology has provided an alternative platform technology for the development of novel low-cost antibody based on evaluating antibiotics derived residues in food matrixes., Methodology/principal Findings: In our current studies, the single chain variable fragments (scFvs) were selected from hybridoma cell lines against sulfadimidine (SM(2)) by using a ribosome library technology. A DNA library of scFv antibody fragments was constructed for ribosome display, and then mRNA-ribosome-antibody (MRA) complexes were produced by a rabbit reticulocyte lysate system. The synthetic sulfadimidine-ovalbumin (SM(2)-OVA) was used as an antigen to pan MRA complexes and putative scFv-encoding genes were recovered by RT-PCR in situ following each panning. After four rounds of ribosome display, the expression vector pCANTAB5E containing the selected specific scFv DNA was constructed and transformed into Escherichia coli HB2151. Three positive clones (SAS14, SAS68 and SAS71) were screened from 100 clones and had higher antibody activity and specificity to SM(2) by indirect ELISA. The three specific soluble scFvs were identified to be the same molecular weight (approximately 30 kDa) by Western-blotting analysis using anti-E tag antibodies, but they had different amino acids sequence by sequence analysis., Conclusions/significance: The selection of anti-SM(2) specific scFv by in vitro ribosome display technology will have an important significance for the development of novel immunodetection strategies for residual veterinary drugs.