1. Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
- Author
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Qian Zhang, Jing Ma, Wen-Ping Luo, Xiaojie Wang, Jiang Wang, Dongmei Tan, Yi Tan, and Hao Liang
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0301 basic medicine ,medicine.medical_specialty ,Small interfering RNA ,Stromal cell ,Amino Acid Transport System y+ ,Amino Acids, Cyclic ,Down-Regulation ,Embryonic Development ,Gene Expression ,lcsh:TX341-641 ,Gestational Age ,Biology ,Article ,Andrology ,03 medical and health sciences ,Mice ,l-amino acid transporter-1 (lat1) ,In vivo ,decidualization ,Leucine ,Pregnancy ,l-amino+acid+transporter-1+%28lat1%29%22">">l-amino acid transporter-1 (lat1) ,Placenta ,Internal medicine ,medicine ,Decidua ,Animals ,Decidual cells ,Embryo Implantation ,RNA, Messenger ,RNA, Small Interfering ,Cells, Cultured ,, ,l<%2Fspan>-amino+acid+transporter-1+%28lat1%29%22">">l -amino acid transporter-1 (lat1)stromal cell ,pregnant mice ,Messenger RNA ,Nutrition and Dietetics ,Leucine transport ,Uterus ,Decidualization ,Amino Acid Transport System y+L ,Biological Transport ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Female ,Stromal Cells ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
Amino acids have an important role in the pre and post implantation of placenta and embryo development. l-type amino-acid transporter 1 (lat1) is responsible for the transportation of large neutral amino acids and is mainly expressed in human fetal liver, placenta, brain, etc. This study is the first to investigate the expression of lat1 in the early pregnancy of mouse uteri and its role in the process of decidualization. Endometrial stromal cells of a mouse model were used to evaluate decidualization from Day 4โ8 of pregnancy in vitro followed by lat1 knock down by small interfering RNA and by a competitive inhibitor of Leucine transport 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). The effects of lat1 on decidualization in vivo were assessed by injecting BCH into the uterine horns. The mRNA and protein expressions of lat1 in the implantation sites were higher than that in the inter-implantation sites and were localized in the luminal and gland epithelium, stromal and decidual cells. Its increased expression (p < 0.05) was associated with artificial decidualization as well as activation of prl expression. Down-regulation of lat1 expression in these cells by siRNA and BCH inhibited the decidual progression in vitro. Inhibition of lat1 transportation by BCH controlled decidual progression in vivo also accompanied the down-regulation of prl, lat1 expression in the decidual area and embryo size on Day 8 of pregnancy. In conclusion, these results revealed that lat1 might play an important role in the decidual progression both in vitro and in vivo.
- Published
- 2016
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