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Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.

The purpose of this study is to establish a novel, simple and rapid surface-enhanced Raman spectroscopy (SERS) detection technology for fenamidone. Fenamidone is a fungicide widely used with curative activity against oomycete diseases. Gold nanoparticles (AuNPs) were prepared by improved trisodium citrate reduction method of chloroauric acid. As a surface-enhanced Raman scattering (SERS) active colloid, the optimized product had the advantages of high sensitivity, reproducibility and chemical stability. The Results showed that the detection limit of fenamidone adsorbed on AuNPs was 0.01 mg/kg. The linear response between SERS intensity and logarithmic concentration was R2 = 0.9562. The method was preliminary applied in tobacco sample and showed its potential to serve as an alternative rapid quantification method of fenamidone residues with simplicity, efficiency, high accuracy and precision.